RESUMO

The aim of this study was to correlate the profile of insemination, uterine status and the presence ofmucus on the day of insemination, with a pregnancy rate of buffalo cows in lowland area in the state of Amapá.This study was conducted at Fazenda general Moto LTDA., 148 km from the town of Macapa. The animals werekept in extensive grazing system. They used 48 animals received the following protocol: Day 0 (D0) to insert theintravaginal device P4 and 2.0 mg BE. At D9 was taken withdrawal of the device and application of 0.5 mg ofPGF2a and 400 IU eCG. In D11 was applied 0.004 mg GRnH and carried out insemination in D12, 64 hoursafter the withdrawal. The diagnosis of pregnancy was performed 30 days after artificial insemination. The datacollected from individual records were tabulated in spreadsheets. And evaluated in the SAS software (2000) 23buffaloes that had a positive pregnancy, 3 a bad AI profile, 18 a good AI profile and 2 excellent AI profile. 25cows got negative pregnancy, among them 4 demonstrated bad AI profile, 21 a good AI profile and no possessedexcellent AI profile. Regarding uterine Status- 46 animals (97.9%) had contracted uterus, however only 22(47.83%) animals were diagnosed with positive penhez. The variable presence of mucus, of 48 buffalos 21(43.75%) had such characteristics. The pregnancy rate was 22.9% (11) for the cows with mucus during theIATF. The variables profile of AI, mucus, uterine status, no positive influence on the pregnancy rate.

Assuntos

RESUMO

The objective of this work was to evaluate the influence of the association of rbST with a protocol ofFTAI on the concentration of progesterone (P4) in in undefined breed goats. Goats were inseminated followingthe 11-day protocole. In D6, the animals were divided into three experimental groups: GI (n = 42) 250 mg rbST,SC, G-II (n = 39) 125 mg rbST, SC, and G- III (n = 41) ( control). P4 hormonal dosages were performed byELISA on D0, D09, D12 and D21. The results show that treatment with somatotropin at two different dosesassociated with an IATF protocol did not affect the serum progesterone concentration.

Assuntos

RESUMO

The objective of this work was to evaluate the influence of the association of rbST with a protocol ofFTAI on the concentration of progesterone (P4) in in undefined breed goats. Goats were inseminated followingthe 11-day protocole. In D6, the animals were divided into three experimental groups: GI (n = 42) 250 mg rbST,SC, G-II (n = 39) 125 mg rbST, SC, and G- III (n = 41) ( control). P4 hormonal dosages were performed byELISA on D0, D09, D12 and D21. The results show that treatment with somatotropin at two different dosesassociated with an IATF protocol did not affect the serum progesterone concentration.(AU)

Assuntos

RESUMO

The aim of this study was to correlate the profile of insemination, uterine status and the presence ofmucus on the day of insemination, with a pregnancy rate of buffalo cows in lowland area in the state of Amapá.This study was conducted at Fazenda general Moto LTDA., 148 km from the town of Macapa. The animals werekept in extensive grazing system. They used 48 animals received the following protocol: Day 0 (D0) to insert theintravaginal device P4 and 2.0 mg BE. At D9 was taken withdrawal of the device and application of 0.5 mg ofPGF2a and 400 IU eCG. In D11 was applied 0.004 mg GRnH and carried out insemination in D12, 64 hoursafter the withdrawal. The diagnosis of pregnancy was performed 30 days after artificial insemination. The datacollected from individual records were tabulated in spreadsheets. And evaluated in the SAS software (2000) 23buffaloes that had a positive pregnancy, 3 a bad AI profile, 18 a good AI profile and 2 excellent AI profile. 25cows got negative pregnancy, among them 4 demonstrated bad AI profile, 21 a good AI profile and no possessedexcellent AI profile. Regarding uterine Status- 46 animals (97.9%) had contracted uterus, however only 22(47.83%) animals were diagnosed with positive penhez. The variable presence of mucus, of 48 buffalos 21(43.75%) had such characteristics. The pregnancy rate was 22.9% (11) for the cows with mucus during theIATF. The variables profile of AI, mucus, uterine status, no positive influence on the pregnancy rate.(AU)

Assuntos

RESUMO

O objetivo deste trabalho foi comparar o resultado de diferentes protocolos de inseminação artificial emtempo fixo (IATF), utilizando-se sêmen sexado, sobre a eficiência reprodutiva em novilhas Girolando. Foramutilizadas 62 novilhas clinicamente hígidas e com escore corporal entre 2,5 e 3,5, divididas aleatoriamente emtrês grupos: controle (n = 21), FSH/LH (n = 21) e eCG (n = 20). Todos os animais receberam o mesmotratamento hormonal para sincronização do estro, consistindo na introdução de dispositivo intravaginal com750mg de progesterona (P4) no dia zero às 17-h, e aplicação de 2-mg de benzoato de estradiol (BE). No dia oitoàs 17-h, foram retirados os implantes e aplicados ml de prostaglandina (0,500-mg cloprostenol). No dia nove,todos os animais receberam 1-mg de benzoato de estradiol (BE) às 17-h. As novilhas foram inseminadas no dia11, às cinco horas, isto é 60-h após a retirada do implante. No dia oito, as novilhas foram distribuídasaleatoriamente em três grupos: grupo controle sem FSH/LH e eCG: grupo FSH/LH 25-UI de FSH e LHaplicados na retirada do implante: grupo eCG 300-UI de eCG aplicados na retirada do implante. As novilhasforam examinadas por ultrassonografia 35 dias após a IA para diagnóstico de gestação e aos 45 dias paraavaliação de perda embrionária. O percentual de prenhez para os grupos controle, FSH/LH e eCG foramrespectivamente 19, 28 e 35%. Os dados foram avaliados pelo teste do qui-quadrado, com nível de significânciade 5%. Não houve diferença significativa entre os grupos. Ao se avaliar a taxa de prenhez entre o grupo controlee o grupo eCG em novilhas que não estavam ciclando, houve uma diferença significativa. Conclui-se que, emanimais cíclicos, a eCG e o FSH/LH não interferiram nas taxas de prenhez. Entretanto, quando comparados comos animais acíclicos ou que tinham folículos menores que 8-mm, a eCG teve um efeito favorável, melhorandosubstancialmente os resultados.

The aim of this study was to compare results of different protocols of timed artificial insemination (TAI)using sexed semen on reproductive efficiency in Girolando cattle. Sixty two heifers were used, being clinicallyhealthy with a body score condition between 2,5 and 3,5, divided randomly into three groups: control (n = 21),FSH/LH (n = 21) and eCG (n = 20). All animals received the same hormonal treatment to synchronize estrus,consisting in inserting na intravaginal device with 750 mg of progesterone (P4) plus 2 mg of estradiol benzoate(EB) on day 0 (5:00 PM). On day 8 (5:00 PM), the device was removed, and 2 ml of prostaglandin (0,5 mg ofcloprostenol) were administered. On day 9 all animals received 1 mg of estradiol benzoate (EB; 5:00 PM), andwere inseminated on day 11 at 5:00 AM, 60 h after device removal. On day 8, heifers were randomly assigned intothree groups. Control group without FSH/LH and eCG; group FSH/LH - 25 IU FSH and LH administered afterdevice removal, group eCG - 300 IU eCG administered after device removal. Heifers were examined byultrasonography 35 days after AI for pregnancy diagnosis and 45 days for evaluation of embryonic death.Pregnancy rates for control, FSH/LH, and eCG groups were, respectively, 19, 28 and 35%. Data were analyzed bychi-square, with 5% significance level. No significant difference was seen between-groups. When control and eCGgroups were, compared in heifers not cycling, a difference in pregnancy rate was observed. It is concluded that incycling animals, eCG and FSH/LH does not interfere with pregnancy rates, however in those heifers that were notcycling or had follicles smaller than 8 mm, eCG had a favorable effect thereby increasing substantially the results.

Assuntos

RESUMO

O objetivo deste trabalho foi comparar o resultado de diferentes protocolos de inseminação artificial emtempo fixo (IATF), utilizando-se sêmen sexado, sobre a eficiência reprodutiva em novilhas Girolando. Foramutilizadas 62 novilhas clinicamente hígidas e com escore corporal entre 2,5 e 3,5, divididas aleatoriamente emtrês grupos: controle (n = 21), FSH/LH (n = 21) e eCG (n = 20). Todos os animais receberam o mesmotratamento hormonal para sincronização do estro, consistindo na introdução de dispositivo intravaginal com750mg de progesterona (P4) no dia zero às 17-h, e aplicação de 2-mg de benzoato de estradiol (BE). No dia oitoàs 17-h, foram retirados os implantes e aplicados ml de prostaglandina (0,500-mg cloprostenol). No dia nove,todos os animais receberam 1-mg de benzoato de estradiol (BE) às 17-h. As novilhas foram inseminadas no dia11, às cinco horas, isto é 60-h após a retirada do implante. No dia oito, as novilhas foram distribuídasaleatoriamente em três grupos: grupo controle sem FSH/LH e eCG: grupo FSH/LH 25-UI de FSH e LHaplicados na retirada do implante: grupo eCG 300-UI de eCG aplicados na retirada do implante. As novilhasforam examinadas por ultrassonografia 35 dias após a IA para diagnóstico de gestação e aos 45 dias paraavaliação de perda embrionária. O percentual de prenhez para os grupos controle, FSH/LH e eCG foramrespectivamente 19, 28 e 35%. Os dados foram avaliados pelo teste do qui-quadrado, com nível de significânciade 5%. Não houve diferença significativa entre os grupos. Ao se avaliar a taxa de prenhez entre o grupo controlee o grupo eCG em novilhas que não estavam ciclando, houve uma diferença significativa. Conclui-se que, emanimais cíclicos, a eCG e o FSH/LH não interferiram nas taxas de prenhez. Entretanto, quando comparados comos animais acíclicos ou que tinham folículos menores que 8-mm, a eCG teve um efeito favorável, melhorandosubstancialmente os resultados.(AU)

The aim of this study was to compare results of different protocols of timed artificial insemination (TAI)using sexed semen on reproductive efficiency in Girolando cattle. Sixty two heifers were used, being clinicallyhealthy with a body score condition between 2,5 and 3,5, divided randomly into three groups: control (n = 21),FSH/LH (n = 21) and eCG (n = 20). All animals received the same hormonal treatment to synchronize estrus,consisting in inserting na intravaginal device with 750 mg of progesterone (P4) plus 2 mg of estradiol benzoate(EB) on day 0 (5:00 PM). On day 8 (5:00 PM), the device was removed, and 2 ml of prostaglandin (0,5 mg ofcloprostenol) were administered. On day 9 all animals received 1 mg of estradiol benzoate (EB; 5:00 PM), andwere inseminated on day 11 at 5:00 AM, 60 h after device removal. On day 8, heifers were randomly assigned intothree groups. Control group without FSH/LH and eCG; group FSH/LH - 25 IU FSH and LH administered afterdevice removal, group eCG - 300 IU eCG administered after device removal. Heifers were examined byultrasonography 35 days after AI for pregnancy diagnosis and 45 days for evaluation of embryonic death.Pregnancy rates for control, FSH/LH, and eCG groups were, respectively, 19, 28 and 35%. Data were analyzed bychi-square, with 5% significance level. No significant difference was seen between-groups. When control and eCGgroups were, compared in heifers not cycling, a difference in pregnancy rate was observed. It is concluded that incycling animals, eCG and FSH/LH does not interfere with pregnancy rates, however in those heifers that were notcycling or had follicles smaller than 8 mm, eCG had a favorable effect thereby increasing substantially the results.(AU)

Assuntos