RESUMO
PURPOSE: To evaluate the expression of urinary biomarkers of inflammation and tissue remodeling in patients with BPH undergoing surgery and evaluate the association of biomarkers with postoperative urodynamic outcomes MATERIALS AND METHODS: We analyzed urine samples from 71 patients treated with TURP from 2011 to 2017. Urinary levels of epidermal growth factor (EGF), matrix-metalloproteinase-1 (MMP-1), interleukin-6 (IL-6), nerve growth factor (NGF) and monocyte-chemoattractant protein-1 (MCP-1) (by commercial ELISA kit) were measured, adjusted by urinary creatinine (Cr) and analyzed according to patients clinical and urodynamic characteristics (baseline and 12-month postoperative urodynamic) RESULTS: MMP-1/Cr levels were significantly higher among subjects with higher detrusor pressure on preoprative urodynamic. MCP-1/Cr levels were significantly higher amongs subjects with preoperative DO. Preoperative levels of NGF/Cr (0.13 vs 0.08, p = 0.005) and MMP-1/Cr (0.11 vs 0.04, p = 0.021) were predictors of persistent DO 12 months after surgery. The following factors were shown to be useful for predicting the persistence of DO in the postoperative period: NGF/Cr, with an AUC of 0.77 (95% CI 0.62-0.92) (p = 0.006), and MMP-1/Cr, with an AUC of 0.72 (95% CI 0.56-0.88) (p = 0.022). CONCLUSIONS: MMP-1/Cr was associated with higher detrusor pressure and MCP-1/CR with DO. NGF/Cr and MMP-1/Cr were shown to be predictors of persistent postoperative DO.
Assuntos
Complicações Pós-Operatórias/epidemiologia , Hiperplasia Prostática/cirurgia , Hiperplasia Prostática/urina , Ressecção Transuretral da Próstata , Doenças da Bexiga Urinária/epidemiologia , Idoso , Biomarcadores/urina , Humanos , Inflamação/complicações , Inflamação/urina , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/fisiopatologia , Valor Preditivo dos Testes , Hiperplasia Prostática/complicações , Hiperplasia Prostática/patologia , Resultado do Tratamento , Doenças da Bexiga Urinária/fisiopatologia , UrodinâmicaRESUMO
Recognizing patients at early phases of chronic kidney disease (CKD) is difficult, and it is even more challenging to predict acute kidney injury (AKI) and its transition to CKD. The gold standard to timely identify renal fibrosis is the kidney biopsy, an invasive procedure not usually performed for this purpose in clinical practice. SerpinA3 was identified by high-resolution-mass-spectrometry in urines from animals with CKD. An early and progressive elevation of urinary SerpinA3 (uSerpinA3) was observed during the AKI to CKD transition together with SerpinA3 relocation from the cytoplasm to the apical tubular membrane in the rat kidney. uSerpinA3/alpha-1-antichymotrypsin was significantly increased in patients with CKD secondary to focal and segmental glomerulosclerosis (FSGS), ANCA associated vasculitis (AAV) and proliferative class III and IV lupus nephritis (LN). uSerpinA3 levels were independently and positively associated with renal fibrosis. In patients with class V LN, uSerpinA3 levels were not different from healthy volunteers. uSerpinA3 was not found in patients with systemic inflammatory diseases without renal dysfunction. Our observations suggest that uSerpinA3 can detect renal fibrosis and inflammation, with a particular potential for the early detection of AKI to CKD transition and for the differentiation among lupus nephritis classes III/IV and V.
Assuntos
Injúria Renal Aguda/urina , Insuficiência Renal Crônica/urina , Serpinas/urina , alfa 1-Antiquimotripsina/urina , Adulto , Sequência de Aminoácidos , Animais , Vasculite Associada a Anticorpo Anticitoplasma de Neutrófilos/urina , Biomarcadores/urina , Progressão da Doença , Diagnóstico Precoce , Feminino , Glomerulosclerose Segmentar e Focal/urina , Humanos , Inflamação/urina , Isquemia/urina , Rim/irrigação sanguínea , Nefrite Lúpica/classificação , Nefrite Lúpica/urina , Masculino , Espectrometria de Massas , Pessoa de Meia-Idade , Pancreatite/urina , Transporte Proteico , Distribuição Aleatória , Ratos , Ratos Wistar , Insuficiência Renal Crônica/diagnóstico , Adulto Jovem , alfa 1-Antitripsina/urinaRESUMO
OBJECTIVE: To evaluate the relationship between the inflammatory profile and mood states in the different phases of the menstrual cycle in soccer players with and without premenstrual syndrome (PMS). METHODS: Data on the menstrual cycle and mood states were collected using the Daily Symptom Report and the Brunel Mood Scale. Cytokine and stress hormone concentrations were measured in urine by flow cytometry before and after a game in the luteal phase and in the follicular phase of one menstrual cycle. RESULTS: In all, 59.6% of the athletes had PMS. The PMS group showed higher concentrations of interleukin (IL)-1ß, IL-6, and IL-8 than the athletes without PMS. After the game, IL-6 decreased in the follicular phase and the luteal phase. The tumor necrosis factor-α levels were higher in the group without PMS during the post-game follicular phase than before the game. In the PMS group, tension was higher in the follicular phase before the game and depression was higher in the pre-game luteal phase than in the group without PMS. The PMS group also presented a negative correlation between depression and IL-10 levels in the pre-game follicular phase. Finally, in the pre-game luteal phase were found positive correlations between growth hormone and IL-10. CONCLUSION: PMS influences the inflammatory condition related to mood states and stress hormones in female soccer players.
Assuntos
Afeto , Ansiedade/psicologia , Citocinas/imunologia , Depressão/psicologia , Inflamação/imunologia , Síndrome Pré-Menstrual/imunologia , Síndrome Pré-Menstrual/psicologia , Futebol , Adolescente , Ansiedade/imunologia , Ansiedade/urina , Atletas , Citocinas/urina , Depressão/imunologia , Depressão/urina , Feminino , Fase Folicular/psicologia , Fase Folicular/urina , Hormônio do Crescimento Humano/urina , Humanos , Inflamação/urina , Interleucina-1beta/imunologia , Interleucina-1beta/urina , Interleucina-6/imunologia , Interleucina-6/urina , Interleucina-8/imunologia , Interleucina-8/urina , Fase Luteal/psicologia , Fase Luteal/urina , Síndrome Pré-Menstrual/urina , Fator de Necrose Tumoral alfa/imunologia , Fator de Necrose Tumoral alfa/urina , Adulto JovemRESUMO
Objective: To evaluate the effects of burnt sugarcane harvesting on the plasmatic and urinary concentrations of the club cell secretory protein (CC16) and inflammatory systemic biomarkers in a group of sugarcane cutters. Methods: Seventy-eight sugar cane workers were evaluated. The plasmatic and urinary concentrations of CC16, a pulmonary damage marker and inflammatory systemic biomarkers were collected at three time points: before, three months after and six months after the onset of the burnt sugarcane harvesting period. All evaluations were performed at â¼7 am, before the daily work shift. In the three-month evaluation, a post-work shift assessment (acute effect) was also performed. Results: The age of the workers was 37.9 ± 11.0 years. The PM2.5 concentrations were 27.0 (23.0-33.0) and 101.0 (31.0-139.5) µg/m3 in the pre harvest and harvest periods, respectively (p < .001). Burnt sugarcane harvesting was associated with a reduction, throughout the work during burnt sugarcane harvesting (subchronic effect), in plasmatic and urinary CC16 concentrations. Acutely, there was a decrease in plasmatic concentrations. There were acute and subchronic increases in inflammatory markers (neutrophils, monocytes) and muscle damage markers (CK and LDH) and a decrease in red blood cells. Conclusions: Harvesting of burnt sugarcane was associated with acute and subchronic reductions in the plasmatic and urinary concentrations of CC16 protein and changes in systemic inflammatory markers.
Assuntos
Poluentes Atmosféricos/efeitos adversos , Produção Agrícola , Exposição por Inalação/efeitos adversos , Exposição Ocupacional/efeitos adversos , Material Particulado/efeitos adversos , Saccharum , Adulto , Biomarcadores/sangue , Biomarcadores/urina , Humanos , Inflamação/sangue , Inflamação/induzido quimicamente , Inflamação/imunologia , Inflamação/urina , Pulmão/efeitos dos fármacos , Pulmão/imunologia , Masculino , Pessoa de Meia-Idade , Monócitos/imunologia , Neutrófilos/imunologia , Uteroglobina/sangue , Uteroglobina/urinaRESUMO
Environmental exposure to pollutants, especially polycyclic aromatic hydrocarbons (PAHs), could lead to carcinogenesis development. However, there is a gap on the mechanisms involved in this effect. Therefore, the aim of this study was to investigate the potential relationship between exposure to environmental air pollution and inflammation process in DNA damage in taxi drivers. This study included 45 taxi drivers and 40 controls; non-smokers composed both groups. Biological monitoring was performed through quantification of urinary 1-hydroxypyrene (1-OHP). ICAM-1 (CD54) expression, NTPDase activity, inflammatory cytokine (IL-1ß, IL-6, IL-10, TNF-α and IFN-γ) levels, and comet and micronucleus assays were evaluated. The results demonstrated that 1-OHP levels, ICAM-1 expression, NTPDase activity, and DNA damage biomarkers (% tail DNA and micronucleus frequency) were increased in taxi drivers compared to the control group (p < 0.01). Moreover, significant associations were found between 1-OHP levels and ICAM-1 expression, % tail DNA, and micronucleus frequency (p < 0.05). Besides, pro-inflammatory cytokine levels were positively correlated to % tail DNA and micronucleus frequency (p < 0.001). Our findings suggest an important association between environmental exposure to air pollution with increase of ICAM-1 expression and NTPDase activity in taxi drivers. Additionally, the multiple regression linear-analysis demonstrated association between IL-6 and DNA damage. Thus, the present study has provided important evidence that, in addition to environmental exposure to air pollutants, the inflammation process may contribute to DNA damage.
Assuntos
Poluentes Atmosféricos , Automóveis , Exposição Ocupacional , Adulto , Biomarcadores/sangue , Biomarcadores/urina , Brasil , Ensaio Cometa , Citocinas/sangue , Dano ao DNA , Monitoramento Ambiental , Humanos , Inflamação/sangue , Inflamação/genética , Inflamação/urina , Masculino , Testes para Micronúcleos , Pessoa de Meia-Idade , Pirenos/urinaRESUMO
Phthalate exposure during pregnancy has been linked to adverse birth outcomes such as preterm birth, and inflammation and oxidative stress may mediate these relationships. In a prospective cohort study of pregnant women recruited early in gestation in Northern Puerto Rico, we investigated the associations between urinary phthalate metabolites and biomarkers of inflammation, including C-reactive protein, IL-1ß, IL-6, IL-10, and TNF-α, and oxidative stress, including 8-hydroxydeoxyguanosine (OHdG) and 8-isoprostane. Inflammation biomarkers were measured in plasma twice during pregnancy (N = 215 measurements, N = 120 subjects), and oxidative stress biomarkers in urine were measured three times (N = 148 measurements, N = 54 subjects) per woman. In adjusted linear mixed models, metabolites of di-2-ethylhexyl phthalate (DEHP) were associated with increased IL-6 and IL-10 but relationships were generally not statistically significant. All phthalates were associated with increases in oxidative stress markers. Relationships with OHdG were significant for DEHP metabolites as well as mono-n-butyl phthalate (MBP) and monoiso-butyl phthalate (MiBP). For 8-isoprostane, associations with nearly all phthalates were statistically significant and the largest effect estimates were observed for MBP and MiBP (49-50% increase in 8-isoprostane with an interquartile range increase in metabolite concentration). These relationships suggest a possible mechanism for phthalate action that may be relevant to a number of adverse health outcomes.
Assuntos
Biomarcadores/urina , Inflamação/urina , Estresse Oxidativo , Ácidos Ftálicos/urina , Adulto , Biomarcadores/sangue , Intervalos de Confiança , Feminino , Humanos , Inflamação/sangue , Modelos Lineares , Ácidos Ftálicos/sangue , Gravidez , Porto Rico , Estatísticas não ParamétricasRESUMO
The proposed curative properties of copper(II)-non-steroidal anti-inflammatory drugs (NSAIDs) have led to the development of numerous copper(II)-NSAID complexes with enhanced anti-inflammatory activity. In this work, the antinociceptive and toxic effects of two new coordination complexes: Cu2(fen)4(caf)2 [fen: fenoprofenate anion; caf: caffeine] and Cu2(fen)4(dmf)2 [dmf: N-N'-dimethylformamide] were evaluated in mice. The antinociceptive effect was evaluated with two models: acetic acid-induced writhing response and formalin test. For the sub-acute exposure, the complexes were added to the diet at different doses for 28days. Behavioral and functional nervous system parameters in a functional observational battery were assessed. Also, hematological, biochemical and histopathological studies were performed. Cu2(fen)4(caf)2 and Cu2(fen)4(dmf)2 significantly decreased the acetic acid-induced writhing response and the licking time on the late phase in the formalin test with respect to the control and fenoprofen salt groups. The sub-acute exposure to Cu2(fen)4(caf)2 complex increased the motor activity, the number of rearings and the arousal with respect to the control and fenoprofen salt groups. These impaired parameters in mice exposed to Cu2(fen)4(caf)2 can be attributable to the presence of caffeine as stimulating agent. On the other hand, all exposed groups decreased the urine pools in the functional observational battery and increased the plasmatic urea. These effects could be due to the decrease in the glomerular filtration caused by NSAIDs. In conclusion, both complexes Cu2(fen)4(dmf)2 and Cu2(fen)4(caf)2 were more potent antinociceptive agents than fenoprofen salt. Sub-acute exposure to different doses of these complexes did not produce significant changes in the parameters that evaluate toxicity.
Assuntos
Dor Abdominal/tratamento farmacológico , Analgésicos/uso terapêutico , Anti-Inflamatórios não Esteroides/uso terapêutico , Complexos de Coordenação/uso terapêutico , Fenoprofeno/uso terapêutico , Inflamação/tratamento farmacológico , Dor Abdominal/sangue , Dor Abdominal/prevenção & controle , Dor Abdominal/urina , Analgésicos/administração & dosagem , Analgésicos/efeitos adversos , Analgésicos/química , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/efeitos adversos , Anti-Inflamatórios não Esteroides/química , Nível de Alerta/efeitos dos fármacos , Cafeína/administração & dosagem , Cafeína/efeitos adversos , Cafeína/química , Cafeína/uso terapêutico , Estimulantes do Sistema Nervoso Central/administração & dosagem , Estimulantes do Sistema Nervoso Central/efeitos adversos , Estimulantes do Sistema Nervoso Central/química , Estimulantes do Sistema Nervoso Central/uso terapêutico , Complexos de Coordenação/administração & dosagem , Complexos de Coordenação/efeitos adversos , Complexos de Coordenação/química , Cobre/administração & dosagem , Cobre/efeitos adversos , Cobre/química , Dimetilformamida/administração & dosagem , Dimetilformamida/química , Relação Dose-Resposta a Droga , Feminino , Fenoprofeno/administração & dosagem , Fenoprofeno/efeitos adversos , Fenoprofeno/química , Insuficiência Hepática/induzido quimicamente , Inflamação/sangue , Inflamação/prevenção & controle , Inflamação/urina , Camundongos , Medição da Dor , Distribuição Aleatória , Insuficiência Renal/induzido quimicamenteRESUMO
Fabry disease is an X-linked inborn error of glycosphingolipid catabolism due to deficient activity of α-galactosidase A that leads to accumulation of the enzyme substrates, mainly globotriaosylceramide (Gb3), in body fluids and lysosomes of many cell types. Some pathophysiology hypotheses are intimately linked to reactive species production and inflammation, but until this moment there is no in vivo study about it. Hence, the aim of this study was to investigate oxidative stress parameters, pro-inflammatory cytokines and Gb3 levels in Fabry patients under treatment with enzyme replacement therapy (ERT) and finally to establish a possible relation between them. We analyzed urine and blood samples of patients under ERT (n=14) and healthy age-matched controls (n=14). Patients presented decreased levels of antioxidant defenses, assessed by reduced glutathione (GSH), glutathione peroxidase (GPx) activity and increased superoxide dismutase/catalase (SOD/CAT) ratio in erythrocytes. Concerning to the damage to biomolecules (lipids and proteins), we found that plasma levels of malondialdehyde (MDA) and protein carbonyl groups and di-tyrosine (di-Tyr) in urine were increased in patients. The pro-inflammatory cytokines IL-6 and TNF-α were also increased in patients. Urinary Gb3 levels were positively correlated with the plasma levels of IL-6, carbonyl groups and MDA. IL-6 levels were directly correlated with di-Tyr and inversely correlated with GPx activity. This data suggest that pro-inflammatory and pro-oxidant states occur, are correlated and seem to be induced by Gb3 in Fabry patients.