RESUMO
OBJETIVE: Several biomarkers of ovarian reserve have been proposed as possible predictors of the response to controlled ovarian stimulation (COS). We aimed to evaluate age, FSH, AMH, antral follicle count (AFC), and ovarian response prediction index (ORPI), as potential predictors of response to COS. METHODS: Cross-sectional study enrolling of 188 infertile women who underwent the first cycle of IVF/ICSI. AFC was evaluated; serum FSH and AMH levels were measured by ELISA. ORPI was calculated as AMH x AFC/patient´s age. RESULTS: As expected, hypo-responder group had less retrieved oocytes, MII, and embryos compared to the good responders. The hyper-response patients were younger, with lower FSH, increased AMH, AFC, and ORPI values. Regarding the assessment of the predictive capacity of ovarian reserve tests, none of them individually or combined showed a good predictive capacity for hypo-response. With respect to the hyper-responder group, individually AMH was the best predictor, while in the multivariable model, ORPI demonstrated the best predictive capacity. Furthermore, patients with serum AMH < 2.09 ng/mL (p25) had fewer AFC than patients with higher AMH values. CONCLUSIONS: Our findings suggest that none of the ovarian reserve tests showed a good predictive capacity for hypo-response, while the ORPI was the strongest predictor of hyper-response in normovulatory infertile women.
Assuntos
Hormônio Antimülleriano/sangue , Infertilidade/terapia , Folículo Ovariano/diagnóstico por imagem , Reserva Ovariana , Indução da Ovulação/métodos , Adulto , Estudos Transversais , Transferência Embrionária , Feminino , Fertilização in vitro , Humanos , Infertilidade/sangue , Testes de Função Ovariana , Gravidez , Taxa de Gravidez , Prognóstico , Estudos Prospectivos , Injeções de Esperma IntracitoplásmicasRESUMO
INTRODUCTION: Lead is a multiple organ toxicant and an oxidative-stress inducer. The effect of Costus afer on metal- induced male reprotoxicity has not been previously carried out, hence this study. The present study investigates the protective effect of Costus afer aqueous leave extract on lead- induced reproductive damages in male albino Wistar rats. METHODS: Adult male albino Wistar rats were weighed and separated into five groups of five rats each. Groups 1 & 2 served as normal and toxic controls receiving deionized and leaded (CH3COO)2Pb.3H2O and water respectively. Groups 3, 4 and 5 were given 750, 1500 and 2250mg/kg of Costus afer orally, respectively while receiving Pb2+ water ad libitum for 28 days. RESULTS: The reproductive and antioxidant parameters obtained from the result served as scientific evidence in the study. The result showed non-significant changes in the absolute and relative weights of epididymis and testes in the Pb Group versus the control. Significant increases were recorded in the sperm analysis, blood lead (7.9±1.02; 1.1±0.01) level (BLL), luteinizing hormone (LH) (8.5±1.4:5.5±0.4), and a decrease in follicle stimulating hormone (FSH) (4.5±2.6:6.5±1.65), with non-significant changes in testosterone (TET) (1.3±0.00:1.6±0.2) in the Pb group compared to the control. CONCLUSION: The treatment with Costus afer exhibited dose-dependent significant changes in testicular oxidative stress, hormonal, sperm analysis and histopathological changes induced by lead. Aqueous leaves extract of Costus afer may be protective against lead induced testicular damage.
Assuntos
Costus/química , Infertilidade/induzido quimicamente , Infertilidade/prevenção & controle , Intoxicação por Chumbo/complicações , Extratos Vegetais/farmacologia , Substâncias Protetoras/farmacologia , Animais , Hormônio Foliculoestimulante/sangue , Infertilidade/sangue , Chumbo/toxicidade , Intoxicação por Chumbo/sangue , Intoxicação por Chumbo/tratamento farmacológico , Intoxicação por Chumbo/patologia , Hormônio Luteinizante/sangue , Masculino , Estresse Oxidativo/efeitos dos fármacos , Folhas de Planta/química , Ratos , Ratos Wistar , Reprodução/efeitos dos fármacos , Análise do Sêmen , Testículo/efeitos dos fármacos , Testículo/fisiologia , Testosterona/sangueRESUMO
OBJECTIVE: To evaluate the predictive efficiency of serum estradiol (E2) concentration in the mid-luteal phase regarding chemical, clinical, and ongoing pregnancies, in patients subjected to IVF/ICSI with fresh embryo transfer. METHODS: One hundred and forty-three patients undergoing IVF/ICSI met all the inclusion criteria for the present study. Most of the patients used antagonists, final maturation was achieved with recombinant chorionic gonadotrophin (HCG), and embryo transfer took place on days 3 to 5, but mostly on day 4. The luteal phase was supplemented with estradiol valerate 6 mg/day and vaginal micronized progesterone 600 mg/day. There was no exclusion of patients in the embryo transfer group due to age or ovarian reserve. All patients with estradiol and chorionic gonadotrophin (ßHCG) dosage on the day of transfer, day 7, were included. We assessed the following variables, initially regarding age: number of eggs collected, formed embryos, embryos transferred, day of transfer, transfer type, estradiol and chorionic gonadotropin. Next, we evaluated these elements at three different ranges of estradiol concentrations (<200 pg/ml, 200-500 pg/ml, and >500 pg/ml), comparing these parameters in pregnant (P) and non-pregnant (NP) patients. RESULTS: Data analysis by age group in P and NP patients showed significant differences in the mean values of the variables E2 and ßHCG, TD7. Mean serum estradiol levels in P and NP in the three age groups were: <35 years, 835/417 p=0.0006, 35-39 years 833/434 p=0.0118, >39 years, 841/394 p=0.0012. There was also a significant difference in pregnancy rates in the group >500 pg/ml of estradiol concentration (63.4%, p=0.0096). The likelihood of chemical and clinical abortions for the estradiol ranges were: 38.46%, involving the two first ranges versus 15.15% for a concentration >500 pg/ml, p=0.0412 and 5.26% for a concentration >900 pg/ml, p=0.0105. The Pearson correlation coefficient for HCG and estradiol was r=0.5108. CONCLUSION: This study showed the prognostic value of E2 in the mid-luteal phase (TD7) for chemical, clinical, and ongoing pregnancies, and its concentration suggested that there is a moderately positive correlation with ßHCG levels.
Assuntos
Estradiol/sangue , Fertilização in vitro/métodos , Infertilidade/diagnóstico , Infertilidade/terapia , Fase Luteal/sangue , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Infertilidade/sangue , Masculino , Idade Materna , Pessoa de Meia-Idade , Indução da Ovulação/métodos , Valor Preditivo dos Testes , Gravidez , Taxa de Gravidez , Testes de Gravidez/métodos , Prognóstico , Injeções de Esperma Intracitoplásmicas/métodos , Adulto JovemRESUMO
La expresión de antígenos (Ags) Lewis depende de alelos heredados en loci independientes, el gen Secretor (SE) que codifica la fucosiltransferasa 2 (FUT2) y el gen Lewis (LE) que codifica la fucosiltransferasa 3 (FUT3). El gen Se codifica una glicosiltransferasa que adiciona una fucosa en la cadena precursora de tipo 1 formando el Ag H en secreciones y fluidos. Como los azúcares inmunodominantes del Ag A y B pueden ser agregados a la cadena H de tipo 1, la FUT2 también controla la expresión de Ag A y B en las secreciones. El gen se es un alelo no funcional. El gen Le codifica una transferasa diferente que adiciona una fucosa en el 2do carbono en el precursor de tipo 1. El alelo le no es funcional. Las FUT2 y FUT3 interactúan para la formación de Ags Lewis en secreciones y fluídos. Los Ags Lewis en los eritrocitos no son en realidad parte integral de la membrana, están adsorbidos sobre la superficie en forma pasiva a partir del plasma. Están ampliamente distribuidos en tejidos humanos, eritrocitos, endotelio, riñón, tracto genitourinario, epitelio gastrointestinal y son receptores para algunos patógenos. Los anticuerpos (Acs) anti-Lewis en general no son clínicamente significativos, aunque se han publicado algunos casos de reacciones transfusionales hemolíticas, enfermedad hemolítica fetoneonatal y rechazo de transplante renal. Este trabajo es una revisión sobre los Ags del Sistema Lewis enfocada hacia sus diferentes funciones biológicas y su importancia en campos variados fuera del Banco de Sangre y la Inmunohematología tradicional.
The expression of Lewis blood group antigens depends on the alleles inherited at independent loci, FUT2 Secretor gene (SE) and FUT3 Lewis gene (LE). The Se and Le alleles encode separate fucosyltransferases that interact to form Lewis antigens in secretions and fluids. The Lewis antigens on red blood cells are not integral to the membrane but are passively adsorbed from the plasma. The allele Se encodes a transferase that adds fucose to type 1 precursor chains in secretions and fluids to form type 1 H antigen. Because A and B terminal sugars may be added to type 1 H chains, FUT2 also controls A and B expression in secretions. The FUT2 allele se gen is a nonfunctional allele. The FUT3 allele Le encodes a transferase that adds a fucose in other position in type 1 H precursor. The FUT3 allele le gen is a nonfunctional allele. The Le antigens are widely distributed in human tissues and fluids and are receptors for some pathogenic organisms. Lewis antibodies are rare and clinically no significant, although there are some reports of hemolytic transfusion reactions, hemolytic disease of the newborn and renal transplant rejection. This review focuses on different biological functions of Lewis antigens and their importance in some fields other than Blood Banks and traditional.
Assuntos
Humanos , Animais , Antígenos do Grupo Sanguíneo de Lewis , Antígenos CD15/genética , Antígenos CD15/imunologia , Antígenos CD15/fisiologia , Aderência Bacteriana , Diferenciação Celular , Neoplasias do Colo/sangue , Infertilidade/sangue , Neoplasias Bucais/sangue , Metástase Neoplásica/ultraestrutura , Neoplasias Ovarianas/sangueRESUMO
The objective of this study was to evaluate whether seasonality affects human-assisted reproduction treatment outcomes. For this, 1932 patients undergoing intracytoplasmic sperm injection (ICSI) were assigned to a season group according to the day of oocyte retrieval: winter (n = 435), spring (n = 444), summer (n = 469) or autumn (n = 584). Analysis of variance was used to compare the ICSI outcomes. The fertilization rate was increased during the spring (winter: 67.9%, spring: 73.5%, summer: 68.7% and autumn: 69.0%; p < 0.01). In fact, a nearly 50% increase in the fertilization rate during the spring was observed (odds ratio 1.45, confidence interval 1.20-1.75; p < 0.01). The oestradiol concentration per number of oocytes was significantly higher during the spring (winter: 235.8 pg/mL, spring: 282.1 pg/mL, summer: 226.1 pg/mL and autumn: 228.7 pg/mL; p = 0.030). This study demonstrates a seasonal variability in fertilization after ICSI, where fertilization is higher during the spring than at any other time.
Assuntos
Infertilidade/terapia , Técnicas de Reprodução Assistida , Análise de Variância , Brasil , Estudos de Coortes , Ectogênese , Transferência Embrionária , Estradiol/sangue , Feminino , Humanos , Infertilidade/sangue , Infertilidade Feminina/sangue , Infertilidade Feminina/terapia , Masculino , Indução da Ovulação , Fotoperíodo , Gravidez , Taxa de Gravidez , Serviços de Saúde Reprodutiva , Estudos Retrospectivos , Estações do Ano , Injeções de Esperma IntracitoplásmicasRESUMO
OBJECTIVE: To assess the effect of antiacrosin antibodies upon proacrosin/acrosin activities and animal fertility. DESIGN: Prospective study. SETTING: Basic research laboratory. PATIENT(S): A gene immunization (GI) model was developed; mice were injected with the sequence encoding human proacrosin (h-proacrosin), cloned in an expression vector. INTERVENTION(S): Subcloning of h-proacrosin in a eukaryotic expression vector (promoter, CMV; leader sequence, alpha-1 antitrypsin; pSF2-Acro); GI of female mice with this plasmid. MAIN OUTCOME MEASURE(S): The following parameters were evaluated: [1] adequate conditions for GI protocols, [2] humoral response to GI with pSF2-Acro, [3] protein regions recognized by the antibodies, and [4] effect of antibodies upon proacrosin/acrosin-ZPA binding and amidase activity, and animal fertility. RESULT(S): Conditions of female mice GI with the proacrosin sequence were established (plasmid purification with anion exchange chromatography and 40 microg of pSF2-Acro per dose) to trigger an immune response, reaching maximum levels at week 9 after the first injection. Antibodies produced by GI recognized human and mouse sperm acrosin systems, inhibited human proacrosin/acrosin interaction with recombinant human ZPA and protease activity, and negatively affected mouse IVF and early embryonic development. In addition, mice immunized with SF2-Acro exhibited a significantly lower size of fetuses. CONCLUSION(S): Antiacrosin antibodies developed by using GI inhibit human proacrosin/acrosin activities and impair mouse fertility.
Assuntos
Acrosina/imunologia , Autoanticorpos/fisiologia , Precursores Enzimáticos/genética , Precursores Enzimáticos/imunologia , Fertilidade/imunologia , Infertilidade/etiologia , Acrosina/genética , Acrosina/metabolismo , Animais , Autoanticorpos/sangue , Autoanticorpos/farmacologia , Desenvolvimento Embrionário/efeitos dos fármacos , Desenvolvimento Embrionário/imunologia , Precursores Enzimáticos/metabolismo , Feminino , Fertilidade/genética , Fertilização/genética , Fertilização/imunologia , Humanos , Imunização/efeitos adversos , Infertilidade/sangue , Infertilidade/imunologia , Infertilidade/veterinária , Masculino , Camundongos , Camundongos Endogâmicos BALB C , GravidezRESUMO
Objetivo: establecer una metodología para evaluar la propiedad de autoesterilidad de 2 adhesivos, el tisuacryl de producción nacional y el histoacryl producido por la B. Brown que se tomó como referencia. Métodos: cada adhesivo se puso en contacto con el inóculo de la cepa seleccionada que provoca su polimerización. Se almacenó un tiempo prudencial y se puso en contacto con sangre fresca no menos de 24 horas. Resultados: en la mayoría de los preparados de polímeros con inóculo sometidos a sangre total, apareció crecimiento bacteriano. Conclusiones: el resultado demostró que ninguno de estos preparados es autoestéril.
Objetive: to establish a methodology to evaluate the autosterility property of 2 adhesives, tisuacryl of national production, and histoacryl produced by B. Brown that was taken as a reference. Methods: every adhesive got in contact with the inoculum of the strain selected that produced its polymerization. It was stored for a prudential time and it got in contact with fresh blood for no less than 24 hours. Results: a bacterial growth appeared in most of the polymer preparations with inoculum subjected to whole blood. Conclusions: the result showed that none of these preparations was autosterile.
Assuntos
Técnicas In Vitro , Infertilidade/microbiologia , Infertilidade/sangueRESUMO
OBJETIVOS: A incompatibilidade ABO está associada com algumas patologia e estudos sugerem também uma possível associação com a infertilidade. O objetivo deste estudo foi verificar se a incompatibilidade ABO entre os casais pode ser um fator contribuinte para o quadro de infertilidade. MATERIAL E MÉTODOS: Foram analisados 161 casais quanto ao grupo sangüíneo ABO e divididos em dois grupos: Grupo 1 - constituído por 72 casais férteis e grupo 2 - constituído por 89 casais inférteis. Análise estatística foi realizada pelos testes Fischers exact test e chi-square test p menor que 0,05. RESULTADOS: Os resultados mostraram um aumento significativo na incompatibilidade ABO nos casais inférteis 38(42,7%) quando comparado com a dos casais férteis 15(25%). Observamos também uma maior freqüência do grupo sangüíneo O nos homens férteis e do grupo B nos homens inférteis. CONCLUSÕES: Os resultados sugerem que a incompatibilidade ABO pode ser um fator contribuinte para a infertilidade, evidenciado pelo aumento significativo da incidência de casais ABO incompatíveis no grupo de casais inférteis
Assuntos
Humanos , Masculino , Feminino , Sistema ABO de Grupos Sanguíneos , Antígenos de Grupos Sanguíneos , Incompatibilidade de Grupos Sanguíneos/complicações , Infertilidade/sangueRESUMO
The objective of the present paper was to assess the presence of hormonal alterations in infertile women with stage I or II endometriosis (Group III, n = 20) compared to fertile women without endometriosis (Group I, n = 14) and to fertile women with endometriosis (Group II, n = 7). Serum levels of FSH, LH, estradiol, TSH, and PRL were measured between days 1 and 5 of the early follicular phase; in the luteal phase, three serum samples were collected for progesterone measurement, and endometrial biopsies were performed. Serum estradiol levels were lower (p = 0.035) in infertile patients with endometriosis than in fertile patients without endometriosis. Six infertile patients with endometriosis presented prolactin levels above 20 ng/ml. This was not observed in the other groups. Luteal insufficiency was more frequent in infertile patients with endometriosis (78.9%) than in fertile patients with (42.9%) or without endometriosis (0%). In a multiple logistic regression analysis, only the presence of endometriosis and infertility was significantly associated with luteal insufficiency. The serum levels of LH, FSH, and TSH were not significantly different among the groups. Luteal insufficiency and altered prolactin secretion were associated with endometriosis, and could be important mechanisms causing infertility in this group of patients.