RESUMO
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , DNA Bacteriano/genética , Infecções por Rickettsia/diagnóstico , Rickettsia rickettsii/genética , Animais , Sequência de Bases , Brasil , Pré-Escolar , Chlorocebus aethiops , Eletroforese em Gel de Ágar , Doenças Endêmicas , Humanos , Masculino , Reação em Cadeia da Polimerase , Infecções por Rickettsia/virologia , Carrapatos/microbiologia , Células VeroRESUMO
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.