RESUMO
Fecal samples from 27 pigs were longitudinally analyzed for Teschovirus A (TV-A), Sapelovirus A (SV-A), and Enterovirus G (EV-G) RNA presence. Suckling piglet fecal samples were negative for the three enteric picornaviruses. However, these picornaviruses were detected in 22/27 weaned pig fecal samples. This study provides new data on TV-A, SV-A, and EV-G infection dynamics.
Assuntos
Infecções por Enterovirus/veterinária , Enterovirus Suínos/isolamento & purificação , Fezes/virologia , Infecções por Picornaviridae/veterinária , Picornaviridae/isolamento & purificação , Doenças dos Suínos/virologia , Teschovirus/isolamento & purificação , Animais , Infecções por Enterovirus/fisiopatologia , Infecções por Enterovirus/virologia , Enterovirus Suínos/classificação , Enterovirus Suínos/genética , Feminino , Estudos Longitudinais , Masculino , Filogenia , Picornaviridae/classificação , Picornaviridae/genética , Infecções por Picornaviridae/fisiopatologia , Infecções por Picornaviridae/virologia , Suínos , Doenças dos Suínos/fisiopatologia , Teschovirus/classificação , Teschovirus/genética , DesmameRESUMO
Infections caused by Human Rhinoviruses (HRVs) account for 25-50% of respiratory illnesses among individuals presenting influenza-like illness (ILI). HRVs could be classified in at least three species: HRV-A, HRV-B, and HRV-C. The HRV-C species has frequently been described among children and has led to severe illness resulting in hospitalization; however, the occurrence among adults is unknown. The aim of this study was to assess the clinical presentation and species distribution of HRV infections in different populations during 2001-2008. A total of 770 samples were collected. Subjects consisted of 136 adults from the general community and 207 health-care workers (2001-2003), 232 renal-transplanted outpatients (2002-2004), 70 children with congenital heart disease (2005) and 125 children from a day-care center (2008). Amplification of HRV genes was performed by reverse transcriptase-polymerase chain reaction (RT-PCR) and followed by sequencing and phylogenetic analysis. HRV was detected in 27.4% of samples (211/770), with 72 children (36.9%) and 139 adults infected (24.2%). A total of 89.61% (138/154) unknown HRV strains were sequenced, and 79.22% (122/138) were analyzed. We identified 74 isolates (60.7%) of the HRV A species, 21 (17.2%) of the HRV B species and 27 isolates (22.1%) of the HRV C species. HRV species A and B caused ILI among adult patients, whereas HRV-C did not. The dynamics of infection among different species deserve further analysis.