RESUMO
An interesting case of pyogenic vertebral osteomyelitis with multiple epidural abscesses caused by non-pigmented Prevotella oralis is reported. The patient was a 68-year-old female who presented to the emergency room (ER) with severe pain and tenderness in her lower back with fever. She had recently undergone esophagogastroduodensoscopy (EGD) for complaints of esophageal reflux, which showed submucosal cyst in the esophagus. Magnetic resonance imaging (MRI) of the thoracic spine revealed multiple spinal epidural abscesses with signal enhancement at the level of T6 and T7, suggestive of vertebral osteomyelitis. Two blood cultures drawn one hour apart grew Prevotella oralis. The body fluid aspirated from the abscesses was also positive for the anaerobic commensal P. oralis. Necrosis associated with the submucosal cyst was implicated as the cause of sepsis and osteomyelitis due to this organism.
Assuntos
Idoso , Feminino , Humanos , Infecções por Bacteroidaceae/complicações , Abscesso Epidural/microbiologia , Osteomielite/microbiologia , Prevotella/isolamento & purificação , Doenças da Coluna Vertebral/microbiologia , Vértebras Torácicas , Infecções por Bacteroidaceae/diagnóstico , Abscesso Epidural/diagnóstico , Osteomielite/diagnóstico , Doenças da Coluna Vertebral/diagnósticoRESUMO
An interesting case of pyogenic vertebral osteomyelitis with multiple epidural abscesses caused by non-pigmented Prevotella oralis is reported. The patient was a 68-year-old female who presented to the emergency room (ER) with severe pain and tenderness in her lower back with fever. She had recently undergone esophagogastroduodensoscopy (EGD) for complaints of esophageal reflux, which showed submucosal cyst in the esophagus. Magnetic resonance imaging (MRI) of the thoracic spine revealed multiple spinal epidural abscesses with signal enhancement at the level of T6 and T7, suggestive of vertebral osteomyelitis. Two blood cultures drawn one hour apart grew Prevotella oralis. The body fluid aspirated from the abscesses was also positive for the anaerobic commensal P. oralis. Necrosis associated with the submucosal cyst was implicated as the cause of sepsis and osteomyelitis due to this organism.
Assuntos
Infecções por Bacteroidaceae/complicações , Abscesso Epidural/microbiologia , Osteomielite/microbiologia , Prevotella/isolamento & purificação , Doenças da Coluna Vertebral/microbiologia , Vértebras Torácicas , Idoso , Infecções por Bacteroidaceae/diagnóstico , Abscesso Epidural/diagnóstico , Feminino , Humanos , Osteomielite/diagnóstico , Doenças da Coluna Vertebral/diagnósticoRESUMO
INTRODUCTION: Culture-independent studies have revealed a broad spectrum of oral bacterial taxa that may be associated with disease. This study investigated the prevalence of three new candidate oral pathogens: Prevotella baroniae, Prevotella multisaccharivorax, and as-yet-uncultivated Bacteroidetes oral clone X083 in primary endodontic infections using a devised culture-independent approach. METHODS: Genomic DNA was isolated from samples taken from 52 teeth with different forms of apical periodontitis and used as template in a taxon-specific 16S rRNA gene-based nested polymerase chain reaction assay to determine the prevalence of the 3 target taxa. RESULTS: Bacteroidetes clone X083, P. baroniae, and P. multisaccharivorax were respectively detected in 81%, 43%, and 38% of the root canals of teeth associated with chronic apical periodontitis, in 60%, 40% and 40% of the canals of teeth with acute apical periodontitis, and in 14%, 24%, and 5% of the pus aspirates from acute apical abscesses. No targeted taxon was positively associated with abscesses or clinical symptoms. Overall, Bacteroidetes clone X083, P. baroniae, and P. multisaccharivorax were found in 50%, 35%, and 25% of the samples taken from primary endodontic infections, respectively. CONCLUSIONS: Findings confirmed that the two newly named species and the uncultivated phylotype targeted in this study are associated with different forms of apical periodontitis, and a pathogenetic or at least an ecologic role is suspected.
Assuntos
Infecções Bacterianas/diagnóstico , Infecções por Bacteroidaceae/diagnóstico , Bacteroidetes/classificação , Doenças da Polpa Dentária/microbiologia , Prevotella/classificação , Doença Aguda , Adolescente , Adulto , Idoso , Bacteroidetes/genética , Doença Crônica , DNA Bacteriano/análise , Cavidade Pulpar/microbiologia , Genoma Bacteriano/genética , Humanos , Pessoa de Meia-Idade , Abscesso Periapical/microbiologia , Periodontite Periapical/microbiologia , Prevotella/genética , RNA Bacteriano/análise , RNA Ribossômico 16S/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Adulto JovemRESUMO
Este trabalho tem por objetivo fazer revisão da literatura e relatar um caso clínico de manchas negras, possivelmente causadas pelos Bacilos produtores de pigmentos negros. Acredita-se que a presença da bactéria Prevotella melaninogênica seja fundamental para que haja este tipo de mancha no esmalte dentário, estando em maior quantidade na microflora das crianças portadoras desta pigmentação. Estas manchas extrínsecas estão sendo associadas ao índice de cárie, higiene oral e com a etiologia da doença periodontal. O caso clínico apresentado é o de uma criança com pigmentação preta no esmalte, principalmente nas faces linguais dos dentes anteriores inferiores, acometendo também de forma mais branda outros dentes da cavidade bucal. O tratamento se deu através da educação e motivação da mãe e da criança quanto a higienização e conseqüente profilaxia com jato de bicabornato.
Assuntos
Humanos , Feminino , Criança , Infecções por Bacteroidaceae/diagnóstico , Infecções por Bacteroidaceae/terapia , Prevotella melaninogenicaRESUMO
Porphyromonas endodontalis has been isolated from the endodontic infections mainly in symptomatic teeth. This study evaluated the occurrence of P. endodontalis in both symptomatic and asymptomatic endodontic infections using 16S rRNA gene-directed polymerase chain reaction. P. endodontalis was detected in 39.5% of the cases (17 of 43 teeth). It was present in 4 of the 6 cases with acute periradicular abscess (66.7%) and in 13 of the 37 other cases (35.1%). The presence of P. endodontalis was associated with an asymptomatic periradicular lesion in 6 cases (25%) and in 10 teeth with tenderness to percussion (52.6%). P. endodontalis was also found in one asymptomatic case without evidence of periradicular pathosis. Our results indicated that, although P. endodontalis is commonly detected in symptomatic cases, it can be present in asymptomatic root canal infections. Further studies should determine if this bacterial species is really an important endodontopathogen.
Assuntos
Infecções por Bacteroidaceae/diagnóstico , Doenças da Polpa Dentária/microbiologia , Genes Bacterianos/genética , Prevotella/classificação , RNA Ribossômico 16S/genética , Adulto , Primers do DNA , DNA Bacteriano/genética , Cárie Dentária/microbiologia , Fístula Dentária/microbiologia , Cavidade Pulpar/microbiologia , Necrose da Polpa Dentária/microbiologia , Amplificação de Genes , Humanos , Abscesso Periapical/microbiologia , Doenças Periapicais/microbiologia , Reação em Cadeia da Polimerase , Prevalência , Prevotella/genéticaRESUMO
BACKGROUND: In this paper, our goal was to determine the optimal isolation conditions, biochemical characterization, and preservation of species of the genus Mobiluncus, associated with bacterial vaginosis in patients attending the family planning clinic. Also, we tried to relate its presence with demographic variables and criteria used in the clinical diagnosis of bacterial diagnosis. METHODS: The specimen from the posterior fornix were collected and transported to the laboratory in a Stuart medium, one at room temperature and the other at 4 degrees C. These samples were inoculated in anaerobic culture media. RESULTS: Of a total of 92 patients studied, 61 (66.3%) were normal, 28 (30.4%) bacterial vaginosis, and 3 (3.3%) had intermediate vaginosis. There was statistically significant relationship only with intrauterine device use (p = 0.00499). The presence of curved rod, using Gram's method, was significantly related with pH (p = 0.00000) positive amines test (p = 0.00000), and the presence of clue cells (p = 0.00000). Mobiluncus was observed in 23 samples (82%), and the majority (15) using RLK agar (cold enrichment technique). With conventional techniques, we identified 12 strains as Mobiluncus curtisii and 3 strains as Mobiluncus mulieris. The strains of Mobiluncus sp. grew better from litmus milk conserved at -30 degrees C. CONCLUSION: Isolating Mobiluncus sp. is fairly easy, if the right media and the techniques are used.
Assuntos
Infecções por Bacteroidaceae , Mobiluncus/isolamento & purificação , Vaginose Bacteriana , Técnicas Bacteriológicas , Infecções por Bacteroidaceae/diagnóstico , Infecções por Bacteroidaceae/epidemiologia , Infecções por Bacteroidaceae/microbiologia , Feminino , Humanos , Vaginose Bacteriana/diagnóstico , Vaginose Bacteriana/epidemiologia , Vaginose Bacteriana/microbiologiaRESUMO
A doença periodontal inflamatória é considerada como resultante da açäo das bactérias contidas na placa dentária. Uma vez que as bactérias inteiras näo säo encontradas na intimidade dos tecidos periodontais é provável que a doença ocorra devido à difusäo dos seus produtos através do epitélio do sulco gengival, provocando uma resposta inflamatória e imune. Assim, torna-se importante identificar esses produtos, principalmente das bactérias que predominam na placa dental, como é o caso do Bacteroides melaninogenicus e suas subespécies, que tem se distinguido como patógenos importantes na doença periodontal. O propósito deste estudo foi verificar a presença de antígenos solúveis de B. melaninogenicus subsp. intermedius nos tecidos gengivais, bem como de anticorpos séricos para o mesmo microrganismo em portadores de doença periodontal...