1.
Adv Exp Med Biol
; 974: 157-165, 2017.
Artigo
em Inglês
| MEDLINE
| ID: mdl-28353232
RESUMO
Pulse radiolabelling of cells with radioactive amino acids is a common method for studying the biosynthesis of proteins. The labelled proteins can then be immunoprecipitated and analysed by electrophoresis and imaging techniques. This chapter presents a protocol for the biosynthetic labelling and immunoprecipitation of pancreatic islet proteins which are known to be affected in psychiatric disorders such as schizophrenia.