RESUMO

OBJECTIVE: We aim to assess the accuracy and effectiveness of the HemoCue 301, a point-of-care (POC) device for measuring hemoglobin levels, and detecting anemia among individuals living in Tumbes, a rural, underserved area in Northern Peru. METHODS: Baseline analysis of a clinical trial aimed at assessing the effect of multi-fortified bread (NCT05103709). Adult women with capillary blood HemoCue 301 readings below 12 g/dL were recruited in coastal city of Tumbes, Peru. A total of 306 women took part of the study, venous blood samples were taken and analyzed with an automated hematology analyzer. Serum samples were used to measure ferritin, serum iron and C reactive protein. RESULTS: Capillary blood measured by the Hemocue 301 has a bias of 0.36 ± 0.93 g/dL respect to the automated Hb. More than 50% of women with normal ferritin values were classified as anemics according to the HemoCue 301. Automated Hb cut-off of 10.8 g/dL [AUC 0.82 (0.77-0.88)] had a specificity of 0.817 and a sensitivity 0.711 while with the HemoCue 301 cut-off of 11.1 g/dL [AUC 0.71 (0.62-0.79)] had a specificity of 0.697 and a sensitivity 0.688. The performance of the automated Hb cut-off was significantly better than the HemoCue (p<0.001). CONCLUSION: Caution must be taken when using POC devices, especially with values around the threshold. Cut-off values found in our study could be used as surrogate means when no confirmatory tests are available. Clinical outcomes should be prioritized when diagnosing iron deficiency anemia in women of reproductive age to ensure proper diagnosis.

Assuntos

RESUMO

Population-based surveys matched by time but using different methodologies for determining hemoglobin (Hb) concentration have shown inconsistencies in estimating anemia prevalence. This study aimed to estimate measurement errors in Hb quantification in HemoCue 201+ using venous blood (VB) and capillary blood both drops (DCB) and pools (PCB), and compare the results against those of a reference method (VB analyzed in hematology analyzers based on the cyanmethemoglobin method). Children (n = 49), adult females (n = 50), and older adults (n = 50) were randomly allocated to donate VB (4 mL) and either DCB (three drops) or PCB (350 µL). Results in HemoCue were analyzed through Bland Altman and Lyn's concordance against Hb concentration by the reference method. A positive average bias (systematic error) was found for the HemoCue (0.31 g/dL) using the same VB samples. This value was then subtracted from all readings carried out in the device. After this adjustment, DCB still produced a positive bias (0.42 ± 0.81 g/dL), and the variation of single results was ±1.6 g/dL (95% CI). PCB and VB performed similarly; the average bias was negligible (-0.02 ± 0.36 and 0.00 ± 0.33 g/dL, respectively) and the variation of the results (95% CI) was ±0.7 g/dL or lower. Lyn's concordance values were 0.86, 0.96, and 0.98 for DCB, PCB, and VB, respectively. Random variation using DCB is too large to approximate the true Hb values, and therefore DCB should be discontinued for diagnosing anemia both in individuals and in populations.

Assuntos

RESUMO

Los principales cambios en la presente guía contemplan una mejor distribución en la presentación de los procedimientos priorizando los que involucran a los niños menores de un año, se actualiza los valores de concentración de hemoglobina en cuanto a la severidad. La atención de la salud requiere de una permanente mejora para prestar un servicio de calidad que incluye información confiable a partir de los instrumentos de medición como en este caso la determinación de la hemoglobina, por lo que esperamos esta segunda edición, sea de utilidad

Assuntos

Assuntos

RESUMO

OBJECTIVE: To evaluate the accuracy and precision of HemoCue 201 (HemoCue) and Masimo Pronto 7 (Masimo) devices for measuring hemoglobin (Hb) in epidemiological studies, having venous blood samples as a gold standard. MATERIAL AND METHODS: We measured Hb concentrations in a field sample of 148 children from one to five years of age. Masimo and HemoCue were used for capillary blood samples and an automatic analyzer for venous blood samples. Regression models with no intercept were constructed to measure precision and predictability, concordance correlations to measure accuracy and precision, and Bland-Altman limits of agreement as well as hierarchical linear models to estimate variance. RESULTS: Both HemoCue and Masimo underestimated Hb concentrations compared to the gold standard. They respectively yielded the following results: regression coefficients of 0.887 and 0.876 with 98.7% and 98.6% predictability; concordance correlation coefficients of 0.183 (p<0.001) and 0.166 (p<0.001); and Bland-Altman variances of -1.51 and -1.62. With regard to Masimo specifically, the three-level Hierarchical Linear Model showed that 57.9% of total variance stemmed from random errors in repeated measures from the same subject. CONCLUSIONS: HemoCue and Masimo measure lower Hb concentrations than the gold standard. Their accuracy and precision levels are comparable. It is essential to ensure proper use of devices through enhanced training of field workers.

Assuntos

RESUMO

To conduct the survey were used 35 (thirty-five) red-browned parrots (A. rhodocorytha), adults, captive, of both genders and clinically healthy, belonging to the live collection of the Museum of Biology Teacher Mello Leitao, located in Santa Teresa, Espírito Santo, Brazil. Harvests were performed in the morning, by puncture of the brachial vein getting 0.5mL of blood stored in EDTA for a period no longer than 6 hours. Blood smears of fresh material were made at collection, stained using the method of May-Grunwald-Giemsa. Analysis of blood elements was done by cell counting in a mirrored Neubauer chamber using Natt and Herrick solution at a ratio of 2:200 as diluent. For the analysis of the methodology, homoglobinometry cyanide hemoglobin using commercial kits by colorimetry on a semi-automatic biochemical analyzer was used. After completion of the statistical data the following parameters were obtained (mean±standard deviation): Erythrocytes (x106/µl): 2.68±0.56; Hemoglobin (g/dl): 14.27±0.69; Hematocrit (%): 53±3.38; Mean corpuscular volume (fl): 206.7±45.82; Mean corpuscular hemoglobin (pg): 56.4±14.46; Mean corpuscular hemoglobin concentration (%): 27.5±1.19; Thrombocytes (x3/µl): 25.8 ± 10.5; Total plasma protein (g/dl) 5.4±0.5; Leukocytes (x103/dl): 3.1±2; Heterophile (/uL): 1937±1676; Lymphocytes (/uL): 1144±599; Monocytes (/uL): 24.4 ± 28.2; Basophils (/uL): 42.2±46.2; Eosinophils (/uL): 11.7±19.9. In the relation between males and females, no significant differences were found in any hematological parameter evaluated.(AU)

Para a realização da presente pesquisa, foram utilizados 35 (trinta e cinco) papagaios-chauás (A. rhodocorytha), adultos, cativos, de ambos os sexos e clinicamente saudáveis, pertencentes ao acervo vivo do Museu de Biologia Professor Mello Leitão, localizado em Santa Teresa, Espírito Santo, Brasil. As coletas foram realizadas pela manhã, por meio da punção da veia braquial, obtendo-se 0,5mL de sangue, armazenado em EDTA por um período máximo de seis horas. Foram confeccionados esfregaços sanguíneos de material fresco no momento da coleta, posteriormente corados pelo método May-Grunwald-Giensa. A análise dos elementos sanguíneos foi feita por meio de contagem celular em câmara de Neubauer espelhada, utilizando-se como diluente a solução de Natt e Herrick na proporção de 2:200. Para a análise de hemoglobinometria, foi utilizada a metodologia do cianeto de hemoglobina, utilizando-se kits comerciais por colorimetria em um analisador bioquímico semiautomático. Depois de realizada a estatística dos dados, obtiveram-se os seguintes parâmetros (média±desvio-padrão): hemácias (x106/µL): 2,68±0,56; hemoglobina (g/dL): 14,27±0,69; hematócrito (%): 53±3,38; volume corpuscular médio (fL): 206,7±45,82; hemoglobina corpuscular média (pg): 56,4±14,46; concentração hemoglobina corpuscular média (%): 27,5±1,19; trombócitos (x3/µL): 25,8±10,5; proteína plasmática total (g/dL) 5,4±0,5; leucócitos (x103/dL): 3,1±2; heterófilos (/µL): 1937±1676; linfócitos (/µL): 1144±599; monócitos (/µL): 24,4±28,2; basófilos (/µL): 42,2±46,2; eosinófilos (/µL): 11,7±19,9. Na relação entre machos e fêmeas, não foi encontrada diferença estatística relevante em nenhum parâmetro hematológico avaliado.(AU)

Assuntos

RESUMO

To conduct the survey were used 35 (thirty-five) red-browned parrots (A. rhodocorytha), adults, captive, of both genders and clinically healthy, belonging to the live collection of the Museum of Biology Teacher Mello Leitao, located in Santa Teresa, Espírito Santo, Brazil. Harvests were performed in the morning, by puncture of the brachial vein getting 0.5mL of blood stored in EDTA for a period no longer than 6 hours. Blood smears of fresh material were made at collection, stained using the method of May-Grunwald-Giemsa. Analysis of blood elements was done by cell counting in a mirrored Neubauer chamber using Natt and Herrick solution at a ratio of 2:200 as diluent. For the analysis of the methodology, homoglobinometry cyanide hemoglobin using commercial kits by colorimetry on a semi-automatic biochemical analyzer was used. After completion of the statistical data the following parameters were obtained (mean±standard deviation): Erythrocytes (x106/µl): 2.68±0.56; Hemoglobin (g/dl): 14.27±0.69; Hematocrit (%): 53±3.38; Mean corpuscular volume (fl): 206.7±45.82; Mean corpuscular hemoglobin (pg): 56.4±14.46; Mean corpuscular hemoglobin concentration (%): 27.5±1.19; Thrombocytes (x3/µl): 25.8 ± 10.5; Total plasma protein (g/dl) 5.4±0.5; Leukocytes (x103/dl): 3.1±2; Heterophile (/uL): 1937±1676; Lymphocytes (/uL): 1144±599; Monocytes (/uL): 24.4 ± 28.2; Basophils (/uL): 42.2±46.2; Eosinophils (/uL): 11.7±19.9. In the relation between males and females, no significant differences were found in any hematological parameter evaluated.(AU)

Para a realização da presente pesquisa, foram utilizados 35 (trinta e cinco) papagaios-chauás (A. rhodocorytha), adultos, cativos, de ambos os sexos e clinicamente saudáveis, pertencentes ao acervo vivo do Museu de Biologia Professor Mello Leitão, localizado em Santa Teresa, Espírito Santo, Brasil. As coletas foram realizadas pela manhã, por meio da punção da veia braquial, obtendo-se 0,5mL de sangue, armazenado em EDTA por um período máximo de seis horas. Foram confeccionados esfregaços sanguíneos de material fresco no momento da coleta, posteriormente corados pelo método May-Grunwald-Giensa. A análise dos elementos sanguíneos foi feita por meio de contagem celular em câmara de Neubauer espelhada, utilizando-se como diluente a solução de Natt e Herrick na proporção de 2:200. Para a análise de hemoglobinometria, foi utilizada a metodologia do cianeto de hemoglobina, utilizando-se kits comerciais por colorimetria em um analisador bioquímico semiautomático. Depois de realizada a estatística dos dados, obtiveram-se os seguintes parâmetros (média±desvio-padrão): hemácias (x106/µL): 2,68±0,56; hemoglobina (g/dL): 14,27±0,69; hematócrito (%): 53±3,38; volume corpuscular médio (fL): 206,7±45,82; hemoglobina corpuscular média (pg): 56,4±14,46; concentração hemoglobina corpuscular média (%): 27,5±1,19; trombócitos (x3/µL): 25,8±10,5; proteína plasmática total (g/dL) 5,4±0,5; leucócitos (x103/dL): 3,1±2; heterófilos (/µL): 1937±1676; linfócitos (/µL): 1144±599; monócitos (/µL): 24,4±28,2; basófilos (/µL): 42,2±46,2; eosinófilos (/µL): 11,7±19,9. Na relação entre machos e fêmeas, não foi encontrada diferença estatística relevante em nenhum parâmetro hematológico avaliado.(AU)

Assuntos

RESUMO

INTRODUCCIÓN La anemia es un problema de salud pública en los países de bajos y medianos ingresos. Al no existir consenso sobre cómo evaluar el nivel de hierro en las poblaciones, la prevalencia de la deficiencia de hierro suele inferirse a partir de la concentración de hemoglobina. El HemoCue es un método recomendado por la OMS para realizar la cuantificación de la hemoglobina a nivel poblacional. OBJETIVO Describir la experiencia inédita de haber utilizado el HemoCue en la red de atención pública de la ciudad de Rosario. MÉTODOS Se realizó un estudio retrospectivo, de corte transversal, en niños de 6 a 42 meses de edad. Las variables de interés fueron el registro del HemoCue en la historia clínica del niño y la toma de conducta (diagnóstica, preventiva o terapéutica) durante la consulta pediátrica. Además se recolectó información (edad, sexo, antecedentes de desnutrición y/o anemia, peso, talla, etc.) con el fin de describir la muestra del estudio. Los datos se analizaron de forma global y según diagnóstico de anemia. Se utilizó el programa R. RESULTADOS Se incluyó un total de 500 niños provenientes de cinco centros de salud. La mediana de la edad fue de 17 meses. A nivel global se registró el HemoCue en un 42% y en alrededor de la mitad de estos casos se tomó algún tipo conducta clínica. En el grupo de niños con anemia (n=242), la toma de conducta basada en el resultado del HemoCue fue mayor (75%, 1C 95%: 660/0-820/0) en comparación con los niños sin anemia (n=258) que contaban con registro del HemoCue (33 0 0). La solicitud de un hemograma de rutina fue la intervención diagnóstica más frecuente y la ìnvestigación para suplementación con hierro por vía oral fue la intervención más utilizada para prevención y/o tratamiento de la anemia. DISCUSIÓN Nuestros hallazgos sugieren que el HemoCue es un método potencialmente útil para el control de la anemia infantil en el sistema público. La evidencia generada a partir de este estudio debería ser considerada en futuras investigaciones

Assuntos

RESUMO

INTRODUCTION: Sildenafil (Viagra®) is a selective phosphodiesterase type 5 (PDE5) inhibitor that block the breakdown of cyclic guanyl monophosphate (cGMP) leading to relaxation of the smooth muscles of the corpus cavernous and an increase in blood flow resulting in penile erection. It is hypothesized that sildenafil will increase the release of oxygen from erythrocytes and shift the oxygen-hemoglobin curve to the right. AIM: The aim of this study was to investigate the effect of varying doses of sildenafil on the p50 of the oxygen-hemoglobin dissociation curve in blood samples from eight (8) healthy adult male volunteers with normal hemoglobin HbAA. METHOD: The hemox-analyzer was used to generate the p50 and the oxygen-hemoglobin dissociation curves. MAIN OUTCOME MEASURES: The effect of different doses of sildenafil on the p50 values and shift of the oxygen-hemoglobin curve were the main outcome measures. RESULT: Sildenafil caused a statistically significant increase in the p50 values and rightward shift of the oxygen-hemoglobin dissociation curve. CONCLUSION: Sildenafil caused a dose-dependent increase in the release of oxygen from the erythrocytes as shown by the increased p50 values and rightward shift of the oxygen-hemoglobin dissociation curve. Ellis SS and Pepple DJ. Sildenafil increases the p50 and shifts the oxygen-hemoglobin dissociation curve to the right.

Assuntos

RESUMO

Introducción: La empresa minera Yanacocha e instituciones públicas y privadas llevaron a cabo el programa "Mejoramiento de la Seguridad Alimentaria como Estrategia de Superación de la Desnutrición Crónica Infantil" en familias rurales de influencia minera del distrito de Baños del Inca para contribuir a mejorar sus condiciones de vida. Se trabajó en cinco líneas de intervención: vivienda, prácticas, crianzas, cultivos y participación. Los resultados estuvieron orientados a salud, producción y participación comunal. Se efectivizaron dos de las tres fases del programa, la primera (2008 - 2009) fue gestión de actividades y productos (capacitación, implementación de huertos familiares, cocinas mejoradas, invernaderos, módulos de cuyes) y la segunda (2010 - 2011) fue gestión por resultados (vivienda saludable, prácticas saludables, familias productivas, familias participativas). El nivel de avance en la ejecución de las actividades por las familias beneficiarias hizo que al 2011 se clasificaran en "familias modelo" (FM) y "familias en proceso" (FEP). Objetivo: Determinar la relación entre estado nutricional de niños menores de 5 años con el tipo de familia beneficiaria del programa social. Diseño: Descriptivo de asociación cruzada, transversal, observacional. Institución: Distrito Baños del Inca – Cajamarca. Participantes: 22 niños y niñas menores de 5 años de edad por cada tipo de familia. Intervenciones: Entre noviembre del 2011 y abril del 2012, y previo consentimiento informado, se aplicaron una encuesta de pesada directa de alimentos, mediciones antropométricas de peso y talla y dosaje de hemoglobina para valorar el estado nutricional de los niños menores de 5 años de ambos tipos de familia. Principales medidas de resultados: Relación entre estado nutricional de niños menores de 5 años según indicadores: consumo (energía, macronutrientes, hierro y vitamina A), antropometría (Peso/talla y Talla/edad) y bioquímico (nivel de hemoglobina) con el tipo de...

Introduction: The mining company Yanacocha and public and private institutions carried out the "Improving Food Security as a Strategy to Overcome Chronic Child Malnutrition" program in rural families influence mining district of Baños del Inca to help improve their living conditions. We worked on five lines of action: housing, practices, breeds, crop and participation, the results were oriented to health, production and community participation. Were effected two of the three program phases, the first (2008-2009) was managing activities and products (training, home gardens, improved stoves, greenhouses, modules guinea pigs) and second (2010-2011) was RBM (healthy housing, healthy practices, productive families, participatory families). The level of progress in the implementation of activities by the beneficiary families made to 2011 were classified under "family model" (FM) and "families in the process" (FEP). Objective: To determine the relationship between nutritional status of children under five years with the type of beneficiary family's social program. Design: Cross descriptive, cross-sectional observational association. Institution: District of Baños del Inca - Cajamarca Participants: 22 children under 5 years of each type of family. Interventions: Between November 2011 and April 2012 and prior informed consent, a survey by direct weighing of food, anthropometric measurements of height and weight and hemoglobin dosage were applied to assess the nutritional status of children under five years of both types of family. Main outcome measures: Relationship between nutritional status of children under five years as indicators: consumption (energy, macronutrients, iron and vitamin A), anthropometry (weight / height and height / age) and biochemical (level of hemoglobin) with the type of family they belonged. Results: Median adequacy of intake of energy, protein, lipids, carbohydrates, iron and vitamin. A to children of FM were 90.1 per cent, 113.7 per cent...

Assuntos

Assuntos

RESUMO

OBJETIVO: El presente trabajo de investigación se lleva a cabo durante la epidemia de Gripe AH1N1 (2009), e intenta determinar aquellos parámetros de laboratorio y clínicos característicos de aquellos pacientes que tuvieron una evolución más grave de la enfermedad. METODO: Fue un trabajo de investigación con un estudio no experimental, observacional, retrospectivo, longitudinal. En los pacientes con neumonía grave resultado de la infección por el virus. Los cuales fueron evaluados y monitorizados en la UCIG del Hospital Loayza, evaluando las características más resaltantes de la enfermedad, y evaluando los cambios que puedan presentarse a nivel clínico y de exámenes de laboratorio, que puedan servir para determinar con antelación aquellos pacientes que tendrán un empeoramiento en la enfermedad, con deterioro de órganos como consecuencia de esta, llámense azoemia, anemia, rabdomiolisis, neumonía intersticial y necesidad de ventilación mecánica con altos requerimientos oxigenatorios y ventilatorios, los que conllevan a una mayor tiempo conectados al ventilador y consecuentemente, fibrosis pulmonar y polineuropatía del paciente crítico, por la necesidad de relajación y sedoanalgesia prolongada. RESULTADOS: Luego de un análisis estadístico se demuestra que la Hemoglobina al 3er día, es la tiene un valor importante en discriminar con mucha certeza la presencia de influenza AH1N1 en los pacientes que inicialmente eran los casos probables. Se estima que los puntos de corte óptimos para esta prueba al 7mo día oscilarían entre 12.45-13.90, siendo el punto de corte optimo el valor de 13.45, en el que la sensibilidad de la prueba alcanza un valor de 83 por ciento y la especificidad de la misma es de 92 por ciento. De la misma manera para la hemoglobina al 3er día se logra alcanzar para el punto de corte de 14.2 con un sensibilidad de 50 por ciento y una especificad de 75 por ciento. CONCLUSIONES: Debemos de considerar a la hemoglobina, como el principal parámetro...

BACKGROUND: This research work was carried out during the epidemic of influenza H1N1 (2009), and attempts to determine the laboratory parameters and clinical characteristics of patients who had the most severe course of the disease. METHOD: It was a research project with a non-experimental, observational, retrospective, longitudinal study in patients with severe pneumonia resulting from infection by the virus, which were evaluated and monitored in UCIG Hospital Loayza. We evaluated the most important characteristics of the disease and changes that may occur clinically and in laboratory tests. This may serve to identify in advance those patients who have a worsening disease with organ impairment as a result of influenza H1N1 such as azotemia, anemia, rhabdomyolysis, interstitial pneumonia or the need for mechanical ventilation with high oxygen requirements. This leads to more time connected to the mechanical ventilation and consequently which may result in pulmonary fibrosis and polyneuropathy. The critical patient may also need additional recovery and prolonged sedation-analgesia. RESULTS: A statistical analysis shows that the hemoglobin on the 3rd day having a significant value in discriminating with much certainty the presence of influenza H1N1 in patients who were initially suspected cases. It is estimated that the optimal cutoff points for this test to be in the range of 12.45-13.90 on the 7th day. The optimal cut-off value is 13.45, in which the sensitivity of the test reaches a value of 83 per cent and specificity 92 per cent. Similarly to the hemoglobin on the 3rd day it achieves the cutoff of 14.2 with 50 per cent sensitivity and a specificity of 75 per cent. CONCLUSIONS: We must consider hemoglobin as the main parameter predictor, where its decline in the first 3 days plays an important role in predicting patients who acquire a torpid and prolonged intensive care unit.

Assuntos

RESUMO

Se diseñó un estudio retrospectivo de casos y controles con el objetivo de determinar si la variación de los niveles de hemoglobina es un factor pronóstico en el reinfarto cardiaco intrahospitalario en pacientes del Servicio de Cardiología del HNAL en el periodo 2010-2012. La muestra de estudio estuvo compuesta de 56 pacientes hospitalizados en el Servicio de Cardiología del HNAL en el periodo 2010 a 2012 con diagnóstico de ingreso de Infarto Agudo de Miocardio, consignado en la historia clínica, con un hemograma basal dentro de las 24 horas desde el inicio de la sintomatología. El diagnóstico de reinfarto se definió según los criterios de GUSTO III. Se calculó la tasa de reinfarto según características clínicas, antecedentes del paciente, y la variación del nivel de hemoglobina. Se consideró el valor de 0.05). En el 62.5 por ciento de los pacientes se registró una disminución de hemoglobina mientras que en el 37.5 por ciento un aumento de hemoglobina; no existió diferencias estadísticamente significativas en la tasa de reinfarto entre quienes aumentó el nivel de hemoglobina (9.5 por ciento; IC 95 por ciento 1,1-30,3 por ciento) y entre quienes disminuyó (20 por ciento; IC 95 por ciento 5,3-34,6 por ciento).

A retrospective case-control study was designed in order to determine whether the change in hemoglobin levels is a prognostic factor in hospital cardiac reinfarction in patients of the cardiology Service HNAL in the period 2010-2012. The study sample consisted of 56 patients hospitalized in the cardiology Department of HNAL in the period 2010-2012 with admission diagnosis of acute myocardial infarction, recorded in the medical record, with a baseline blood count within 24 hours of onset of symptoms. The diagnosis of reinfarction was defined according to criteria GUSTO III. Reinfarction rate was calculated according to clinical characteristics, patient history, and variation in hemoglobin. The value of 0.05) does not exist. In 62.5 per cent of patients had a decrease in hemoglobin while 37.5 per cent increased hemoglobin; there is no significant difference in the rate of reinfarction among those who increased hemoglobin level (9.5 per cent; CI 95 per cent 1.1 to 30.3 per cent) and those who declined (20 per cent; CI 95 per cent 5.3 to 34.6 per cent).

Assuntos

RESUMO

Objetivos: Determinar la variación de la hemoglobina sérica en pacientes con insuficiencia renal crónica terminal hemodializados que reciben eritropoyetina. Material y métodos: Se realizó un estudio observacional, descriptivo retrospectivo de casos. Se revisaron 154 historias clínicas de pacientes con insuficiencia renal crónica terminal que recibieron terapia con eritropoyetina en el servicio de nefrología del Hospital Militar Central en el periodo comprendido de Enero del 2009 a Diciembre del 2012. Resultados: El 62.3 por ciento eran varones, y el 37.7 por ciento fueron mujeres. La media de la edad global fue de 61.4+/-15.6 años, con una mínima de 32 años y una máxima de 91 años. La mayoría de los pacientes provenían de provincias (64.9 por ciento), y eran casados (77.3 por ciento). el motivo de hemodiálisis de mayor frecuencia fue la diabetes (37.7 por ciento), la patología asociada más frecuente fueron las cardiovasculares (31. 8 por ciento). El 87.7 por ciento se hemodializaba mas de tres veces por semana. El 86.4 por ciento llevaban más de dos años dializándose. El 57.8 por ciento de los pacientes tuvieron variación de la hemoglobina mayor o igual de 1gr por ciento. La frecuencia de mortalidad fue del 8.4 por ciento. La media de la hemoglobina al inicio fue de 9.9gr por ciento, y luego de la terapia con eritropoyetina llegó a 11.02gr por ciento, siendo esta diferencia estadísticamente significativa (P<0.05). Encontramos una asociación estadísticamente significativa de mortalidad con variaciones de hemoglobina menores a 1gr por ciento (P<0.05). Conclusiones: Existe una diferencia estadísticamente significativa de variación de la hemoglobina sérica en pacientes con insuficiencia renal crónica terminal hemodializados que reciben eritropoyetina (P<0.05). La mayoría de pacientes provenían de provincias, casados. El motivo de hemodiálisis de mayor frecuencia fue la diabetes, la patología asociada de mayor frecuencia fueron las enfermedades cardiovasculares...

Assuntos

RESUMO

Introducción: una medición fundamental en la selección de los donantes de sangre es la concentración de hemoglobina; sin embargo se han reportado resultados variables dependiendo de la metodología utilizada para su cuantificación. Métodos: estudio descriptivo transversal. Se cuantificó la hemoglobina en 70 donantes mediante el Compolab y el Sysmex XE2100. Además se determinó la hemoglobina reticulocitaria y se aplicó una encuesta sobre hábito tabáquico y actividad física. La concordancia entre los parámetros se evaluó mediante elcoeficiente de correlación intraclase. Los datos se analizaron en SPSS.Resultados: la hemoglobina de eritrocitos maduros fue estadísticamente más alta en hombres y en fumadores. No hubo diferencias según grupo etario ni actividad física. Tuvo buena concordancia entre los resultados del Compolab y el Sysmex yun bajo coeficiente de correlación intraclase entre la hemoglobina reticulocitaria y la de eritrocitos maduros. Conclusiones: la selección del donante puede basarse en la hemoglobina dada por el hemoglobinómetro, cuyas cuantificaciones son intercambiables con las del Sysmex XE2100.

Introduction: hemoglobin quantification is fundamental in the selection of blood donors, but has reported varying results depending on the methodology used for its determination.Methods: cross-sectional study. Hemoglobin was quantitated in 70 donors by the Sysmex XE2100 and Compolab. We also determined reticulocyte hemoglobin, We surveyed about smoking and physical activity. The correlation between parameters was assessed using the intraclass correlation coefficient. Data were analyzed in SPSSResults: the mature erythrocyte hemoglobin was statistically higher in men and in smokers. There were no differences by age group and physical activity. There was good agreement between the results of Compolab and Sysmex. There was a low intraclass correlation coefficient between reticulocyte hemoglobin and mature erythrocyte hemoglobin.Conclusions: the donor selection may be based on hemoglobin given by hemoglobinometer whose quantifications are interchangeable with the Sysmex XE2100.

Introdução: a medição fundamental na seleção de doadores de sangue é a hemoglobina, mas relataram resultados variáveis dependendo do método utilizado para a quantificação.Métodos: a hemoglobina foi quantificada em 70 doadores, e pelo Sysmex XE2100 Compolab. Também determinou a hemoglobina de reticulócitos, foram pesquisadossobre o tabagismo e atividade física. A correlação entre os parâmetros foi avaliada pelo coeficiente de correlação intraclasse. Os dados foram analisados no SPSSResultados: a hemoglobina de eritrócitos maduros foi estatisticamente maior em homens e em fumantes. Não houve diferenças por faixa etária e atividade física. Houve boa concordância entre os resultados de Compolab e Sysmex. Houve um baixo coeficiente de correlação entre hemoglobina e reticulócitos eritrócito maduro.Conclusões: a seleção dos doadores pode ser baseada em hemoglobina dada pelo hemoglobinômetro cujas quantificações são permutáveis com a XE2100 Sysmex.

Assuntos

Assuntos

RESUMO

BACKGROUND: The tests used for anemia screening in blood donors are based on fingerstick samples, leading to discomfort and complaints. The aim of this study was to analyze the feasibility of occlusion spectroscopy method in blood banks and to compare the method with fingerstick hemoglobinometer and hemoglobin (Hb) determination on an automatic blood analyzer. STUDY DESIGN AND METHODS: The study enrolled 205 consecutive volunteer blood donors. Samples were collected by fingerstick and venous punction to determine Hb level by a Hemocue Hb201+ (Hb-F) and automatic blood analyzer (Hb-V) and compare to the noninvasive Hb determination by occlusion spectroscopy using NBM200 system (Hb-NI). The percentage errors of Hb-F and Hb-NI of all donors as well as stratified by sex, weight, and age levels were compared to Hb-V as reference values using Wilcoxon signed rank test. RESULTS: The results obtained with Hb-F showed significant errors (p<0.001) in the general group as well as when stratified by sex, weight, and age groups, above values obtained with Hb-V. Hb-NI showed significant errors only in females (p=0.026) and weight level of 61 to 70kg (p=0.034), below Hb-V values. CONCLUSIONS: Hb-NI seems to be a good method in terms of precision and feasibility for anemia screening of blood donors as well as being much more comfortable for donors.

Assuntos

RESUMO

El Estudio de Control y Complicaciones de la Diabetes (DCCT) y el Estudio Prospectivo de Diabetes en el Reino Unido (UKPDS) establecieron la importancia de la hemoglobina A1c (Hb A1c) como un predictor de consecuencias en pacientes con diabetes mellitus. En 1994, la Asociación Americana de Diabetes comenzó a recomendar metas específicas para Hb A1c, pero la falta de comparación entre los ensayos limitó la capacidad de los médicos para usar estos objetivos. El Programa de Estandarización Nacional de Hemoglobina Glicosilada (NGSP) fue implementado en 1996 para estandarizar los resultados de la Hb A1c según los estudios DCCT / UKPDS. El NGSP certifica a los fabricantes de métodos de Hb A1c como trazables al DCCT. Los criterios de certificación se han reforzado con el tiempo y el NGSP ha trabajado con el Colegio Americano de Patólogos en establecer requisitos estrictos para la aptitud de los ensayos. Como resultado, la variabilidad de los valores de la Hb A1c entre los laboratorios clínicos se ha reducido considerablemente. La Federación Internacional de Química Clínica (IFCC) ha desarrollado un sistema de referencia para la Hb A1c , que facilita la trazabilidad de la metrología a un orden superior. El NGSP mantiene la trazabilidad a la red de la IFCC a través de continuas comparaciones de muestras. Ha habido controversias en relación a si el informe de los resultados de Hb A1c debe ser en unidades IFCC o NGSP, o una estimación promedio de glucosa. Los diferentes países se encuentran tomando esta decisión. La variabilidad entre los resultados de Hb A1c se ha reducido considerablemente. No todos los países informarán Hb A1c en las mismas unidades, pero se han establecido ecuaciones que permiten la conversión entre diferentes unidades. Ahora se recomienda Hb A1c para el diagnóstico de la diabetes, acentuando aún más la necesidad de un ensayo de óptimo rendimiento. El NGSP proseguirá sus esfuerzos para mejorar los tests de Hb A1c asegurando que se cumplan las necesidades clínicas.

BACKGROUND: The Diabetes Control and Complications Trial (DCCT) and United Kingdom Prospective Diabetes Study (UKPDS) established the importance of hemoglobin A1c (Hb A1c) as a predictor of outcome in patients with diabetes mellitus. In 1994, the American Diabetes Association began recommending specific Hb A1c targets, but lack of comparability among assays limited the ability of clinicians to use these targets. The National Glycohemoglobin Standardization Program(NGSP) was implemented in 1996 to standardize Hb A1c results to those of the DCCT/UKPDS. CONTENT: The NGSP certifies manufacturers of Hb A1c methods as traceable to the DCCT. The certification criteria have been tightened over time and the NGSP has worked with the College of American Pathologists in tightening proficiency-testing requirements. As a result, variability of Hb A1c results among clinical laboratories has been considerably reduced. The IFCC has developed a reference system for Hb A1c that facilitates metrological traceability to a higher order. The NGSP maintains traceability to the IFCC network via ongoing sample comparisons. There has been controversy over whether to report Hb A1c results in IFCC or NGSP units, or as estimated average glucose. Individual countries are making this decision. SUMMARY: Variability among Hb A1c results has been greatly reduced. Not all countries will report Hb A1c in the same units, but there are established equations that enable conversion between different units. Hb A1c is now recommended for diagnosing diabetes, further accentuating the need for optimal assay performance. The NGSPwill continue efforts to improve Hb A1c testing to ensure that clinical needs are met.

Assuntos

RESUMO

O objetivo deste artigo é descrever o processo de avaliação da utilização de hemoglobinometria de resultado imediato em diferentes regiões e cenários do Sistema Único de Saúde, utilizando hemoglobinômetro portátil, de tecnologia nacional. Como metodologia, após validação do hemoglobinômetro em campo, contra equipamentos validados de bancada e portátil, em sangue venoso e periférico, foram realizadas avaliações da utilização do equipamento em unidades básicas de saúde tradicional e de saúde da família, na cidade de São Paulo. Foram realizadas avaliações sob a forma de campanhas em Ilhabela (SP), Santa Luzia do Itanhi (SE), Afuá (PA) e Macapá (AP). Em todas as localidades foram realizadas medições diagnósticas, prescrito e fornecido sulfato ferroso aos anêmicos, com monitoramento na metade e ao final do tratamento de 12 semanas. As prevalências e taxas de recuperação de anemia encontradas foram 26,4% e 78,9%; 22,4% e 79%; e 54% e 60%, respectivamente. Conclui-se que a utilização de hemoglobinometria imediata com equipamento nacional de baixo custo gerou agilidade no diagnóstico e tratamento, com taxas de recuperação similares em diferentes regiões do país

Assuntos

RESUMO

Iron deficiency anemia is the most common nutritional deficiency among children. This cross-sectional, descriptive and quantitative study is part of a multicenter project, which verified the prevalence of anemia in children aged three to 12 months, treated by a health service unit in Ribeirão Preto, SP, Brazil. Interviews with mothers and determining hemoglobin dosage were carried out with 121 children who participated in the study. Two international criteria were adopted as parameters of anemia according to the children's age. Descriptive statistics, measures of central tendency and associations were used for data analysis. The prevalence of anemia among 69 children aged three to 5 months was 20.2% and 48.0% among 52 children aged six to 12 months. The total prevalence of anemia was 32.2%. There was significant association between anemia and children's age, and anemia and the consumption of liquid cow's milk.

Assuntos