RESUMO

A D-glucose/D-mannose specific lectin from seeds of Canavalia grandiflora (ConGF) was purified by affinity chromatography on Sephadex G-50. By SDS-PAGE ConGF yielded three protein bands with apparent molecular masses of 29-30 kDa (alpha chain), 16-18 kDa (beta fragment) and 12-13 kDa (gamma fragment), like other related lectins from the genus Canavalia (Leguminosae). ConGF strongly agglutinates rabbit erythrocytes, has a high content of ASP and SER, and its N-terminal sequence (30 residues) is highly similar to the sequences of other related lectins from subtribe Diocleinae.

Assuntos

Fabaceae/química , Lectinas/isolamento & purificação , Sementes/química , Aminoácidos/metabolismo , Animais , Cromatografia de Afinidade , Eletroforese em Gel de Poliacrilamida , Eritrócitos/metabolismo , Fabaceae/genética , Fabaceae/metabolismo , Haptenos/metabolismo , Hemaglutinação , Humanos , Lectinas/química , Lectinas/metabolismo , Lectinas de Plantas , Coelhos