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Rev Latinoam Microbiol ; 35(3): 259-65, 1993.
Artigo em Espanhol | MEDLINE | ID: mdl-8047728

RESUMO

Three extracts from Saccharomyces cerevisiae were obtained by salting out with ammonium sulfate, these were I-G, EFRL-II-G and III-G. Fraction EFRL-II-G showed the highest photoreactivating activity on DNA str2000 irradiated with far UV light. However, the same fraction did not reactivate DNA str2000 previously inactivated by near UV irradiation. We think that the inactivation by near-UV was not due to photochemically-formed pyrimidine dimers. Decrease in spontaneous mutation frequency of cells transformed with DNA str2000 irradiated with near-UV light, was the same with the DNA treated with active or heat inactivated EFRL-II-G, therefore we may conclude that DNA lesions responsible for the effect are difference to pyrimidine dimers.


Assuntos
Dano ao DNA , Reparo do DNA , DNA Bacteriano/efeitos da radiação , Haemophilus influenzae/genética , Transformação Bacteriana/efeitos da radiação , Raios Ultravioleta , Desoxirribodipirimidina Fotoliase/isolamento & purificação , Desoxirribodipirimidina Fotoliase/metabolismo , Proteínas Fúngicas/isolamento & purificação , Proteínas Fúngicas/metabolismo , Haemophilus influenzae/efeitos da radiação , Mutagênese , Dímeros de Pirimidina/química , Saccharomyces cerevisiae/enzimologia
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