RESUMO
The current study aimed at the determination of the impact of obesity on the salivary microbiome in adolescents. Sixty subjects ranging 14-17 years old were enrolled (obese: n = 30-50% females, and normal weight: n = 30-50% females). Stimulated saliva was collected for denaturing gradient gel electrophoresis (DGGE) band patterns and massive 16S rRNA gene sequencing using the Ion Torrent platform. Overall, data analysis revealed that male subjects harbored a higher diverse salivary microbiome, defined by a significant higher richness (32.48 versus 26.74) and diversity (3.36 versus 3.20), higher Simpson values (0.96 versus 0.95) and distinct bacterial community structure considering either sex or condition (p < 0.05). Bacterial community fingerprinting analysis in human saliva showed a positive correlation with increased body mass index (BMI) in adolescents. Veillonella, Haemophilus and Prevotella occurrence was found to be affected by BMI, whereas Neisseria and Rothia occurrence was significantly impacted by sex in obese subjects. Our findings suggest that male and female adolescents may harbor a naturally distinct salivary microbiota and that obesity may specifically have an impact on their oral bacterial community. The potential dysbiotic oral microbiome in obese adolescents raises new insights on the etiology and prevention of future conditions in these populations.
Assuntos
Bactérias/classificação , Bactérias/isolamento & purificação , Microbiota/genética , Obesidade/microbiologia , Saliva/microbiologia , Adolescente , Bactérias/genética , Eletroforese em Gel de Gradiente Desnaturante , Feminino , Haemophilus/isolamento & purificação , Humanos , Masculino , Micrococcaceae/isolamento & purificação , Neisseria/isolamento & purificação , Prevotella/isolamento & purificação , RNA Ribossômico 16S/genética , Veillonella/isolamento & purificaçãoRESUMO
Pyogenic osteomyelitis seldom affects the spine (2-7%). It is diagnosed by the isolation of a bacterial agent in the vertebral body, the intervertebral disks or from paravertebral or epidural abscesses. We report a retrospective study of ten patients who attended a medical clinic with this disease to emphasize its unusual presentation and difficult diagnosis. Lumbar and dorsal spine were the most common sites affected. Dorsolumbar pain and paraparesis were the most frequent symptoms. Staphylococcus aureus were isolated in eight patients, Escherichia coli in one and Haemophilus sp. in other Leukocytosis was observed in only three patients. Erythrocyte sedimentation rate was higher than 100 mm in the first hour in two patients. Typical images of pyogenic vertebral osteomyelitis were observed in all these patients with magnetic resonance imaging. The main complications were paravertebral and epidural abscesses that were found in five patients. One patient also presented an empyema, seven of them initially received empiric medical treatment, and later specific antibiotics according to the culture and sensitivity results. The rest of the patients were initially treated according to the sensitivity of the isolated germ. Surgical intervention was performed in two patients to drain soft tissue involvement, and in two other to stabilize the spine. All four surgical patients had a full recovery. This report is intended to point out that in patients with dorsolumbar pain and neurological symptoms pyogenic vertebral osteomyelitis is a possible diagnosis and has to be treated without delay.
Assuntos
Abscesso/diagnóstico , Osteomielite/diagnóstico , Doenças da Coluna Vertebral/diagnóstico , Abscesso/microbiologia , Abscesso/terapia , Adulto , Idoso , Biópsia por Agulha Fina , Escherichia coli/isolamento & purificação , Feminino , Haemophilus/isolamento & purificação , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Osteomielite/microbiologia , Osteomielite/terapia , Estudos Retrospectivos , Doenças da Coluna Vertebral/microbiologia , Doenças da Coluna Vertebral/terapia , Staphylococcus aureus/isolamento & purificação , Tomografia Computadorizada por Raios XRESUMO
La osteomielitis vertebral piógena (OVP) es una localización poco frecuente (2-7%) Se confirma con el aislamiento de un microorganismo de una vértebra, disco intervertebral, absceso epidural o paravertebral. Se describe una serie de casos por la infrecuente presentación de esta enfermedad, que puede ser consulta inicial en los servicios de clínica médica y por su sintomatología inespecífica que supone una dificultad diagnóstica. Tanto la columna lumbar como la dorsal fueron los sitios más afectados. El dolor dorsolumbar y la paraparesia fueron los síntomas más frecuentes de presentación. En ocho pacientes se aislaron Staphylococcus aureus, en uno Escherichia coli y en el restante Haemophylus sp. Se observó leucocitosis sólo en tres pacientes, y en dos velocidad de sedimentación globular mayor de 100 mm/h. Los diez pacientes presentaron imágenes características de osteomielitis vertebral piógena en la resonancia nuclear magnética. Dentro de las complicaciones, los abscesos paravertebrales y epidurales fueron los más frecuentes (en cinco enfermos). Además, un paciente presentó empiema pleural. De los diez pacientes de esta serie, siete recibieron inicialmente tratamiento médico empírico y luego específico para el germen aislado. En los restantes el tratamiento fue guiado de acuerdo al antibiograma. A dos enfermos fue necesario realizarles laminectomía descompresiva por compromiso de partes blandas y a otros dos estabilización quirúrgica por inestabilidad espinal, observándose buena evolución en todos los casos. Esta serie demuestra que, ante un paciente con dolor dorsolumbar y síntomas neurológicos se deberá tener en cuenta esta entidad para evitar un retraso en el tratamiento.
Pyogenic osteomyelitis seldom affects the spine (2-7%). It is diagnosed by the isolation of a bacterial agent in the vertebral body, the intervertebral disks or from paravertebral or epidural abscesses. We report a retrospective study of ten patients who attended a medical clinic with this disease to emphasize its unusual presentation and difficult diagnosis. Lumbar and dorsal spine were the most common sites affected. Dorsolumbar pain and paraparesis were the most frequent symptoms. Staphylococcus aureus were isolated in eight patients, Escherichia coli in one and Haemophilus sp. in other Leukocytosis was observed in only three patients. Erythrocyte sedimentation rate was higher than 100 mm in the first hour in two patients. Typical images of pyogenic vertebral osteomyelitis were observed in all these patients with magnetic resonance imaging. The main complications were paravertebral and epidural abscesses that were found in five patients. One patient also presented an empyema, seven of them initially received empiric medical treatment, and later specific antibiotics according to the culture and sensitivity results. The rest of the patients were initially treated according to the sensitivity of the isolated germ. Surgical intervention was performed in two patients to drain soft tissue involvement, and in two other to stabilize the spine. All four surgical patients had a full recovery. This report is intended to point out that in patients with dorsolumbar pain and neurological symptoms pyogenic vertebral osteomyelitis is a possible diagnosis and has to be treated without delay.
Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Abscesso/diagnóstico , Osteomielite/diagnóstico , Doenças da Coluna Vertebral/diagnóstico , Abscesso/microbiologia , Abscesso/terapia , Biópsia por Agulha Fina , Escherichia coli/isolamento & purificação , Haemophilus/isolamento & purificação , Imageamento por Ressonância Magnética , Osteomielite/microbiologia , Osteomielite/terapia , Estudos Retrospectivos , Doenças da Coluna Vertebral/microbiologia , Doenças da Coluna Vertebral/terapia , Staphylococcus aureus/isolamento & purificação , Tomografia Computadorizada por Raios XAssuntos
Doenças Transmissíveis Emergentes/epidemiologia , Infecções por Haemophilus/epidemiologia , Brasil/epidemiologia , Criança , Pré-Escolar , Doenças Transmissíveis Emergentes/diagnóstico , Doenças Transmissíveis Emergentes/microbiologia , Surtos de Doenças , Evolução Fatal , Feminino , Febre/etiologia , Haemophilus/classificação , Haemophilus/genética , Haemophilus/isolamento & purificação , Infecções por Haemophilus/diagnóstico , Infecções por Haemophilus/microbiologia , Humanos , Masculino , Púrpura/etiologiaRESUMO
In a smoking adult with a lung mass, brain masses are usually diagnosed as brain metastases of lung origin. Nevertheless, differential diagnosis between cerebral abscesses cannot be performed based on clinical symptoms or imaging technologies, and histological diagnosis is essential. This case illustrates the advisability of always obtaining histological diagnosis of the primary tumor and/or cerebral lesion before introducing any oncological treatment.
Assuntos
Abscesso/microbiologia , Encefalopatias/microbiologia , Infecções por Haemophilus/microbiologia , Haemophilus/isolamento & purificação , Pneumopatias/microbiologia , Abscesso/diagnóstico , Abscesso/terapia , Antibacterianos/uso terapêutico , Encefalopatias/diagnóstico , Encefalopatias/terapia , Terapia Combinada , Diagnóstico Diferencial , Infecções por Haemophilus/diagnóstico , Infecções por Haemophilus/terapia , Humanos , Pneumopatias/diagnóstico , Pneumopatias/terapia , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios XRESUMO
In a case series of 152 children aged from 2 to 132 months will pleural emphema from a paediatric tertiary hospital in Luanda, Angola between September 2004 and March 2005, the authors found a high prevalence of anaemia and malnutrition. The most prevalent bacteria in pleural fluid were: D pneumoniae, Haemophyllus and S aureus. The median for hospital stay was 25 days. The lethality was 7.8% and was not statistically associated with malnutrition, although this variable was associated, in multivariate analysis, with prolonged hospitalization time.
Assuntos
Empiema Pleural/epidemiologia , Empiema Pleural/fisiopatologia , Hospitais Pediátricos/estatística & dados numéricos , Tempo de Internação/estatística & dados numéricos , Adulto , Anemia/epidemiologia , Angola/epidemiologia , Criança , Transtornos da Nutrição Infantil/epidemiologia , Pré-Escolar , Empiema Pleural/microbiologia , Empiema Pleural/mortalidade , Feminino , Haemophilus/isolamento & purificação , Infecções por Haemophilus/epidemiologia , Infecções por Haemophilus/microbiologia , Infecções por Haemophilus/mortalidade , Infecções por Haemophilus/fisiopatologia , Humanos , Lactente , Transtornos da Nutrição do Lactente/epidemiologia , Masculino , Infecções Pneumocócicas/epidemiologia , Infecções Pneumocócicas/microbiologia , Infecções Pneumocócicas/mortalidade , Infecções Pneumocócicas/fisiopatologia , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/mortalidade , Infecções Estafilocócicas/fisiopatologia , Staphylococcus aureus/isolamento & purificação , Streptococcus pneumoniae/isolamento & purificaçãoRESUMO
Cystic Fibrosis (CF) is characterized by a dysfunction of the exocrine secretion glands. The first symptoms often appear in the respiratory system which constitutes one of the most important morbimortality causes in these patients. Chronic respiratory tract colonization is caused mainly by bacteria such as Staphylococcus aureus, Haemophilus spp. and Pseudomonas aeruginosa. Respiratory samples from patients with CF (age group: 4 months to 11 years) were analyzed from November 2001 to August 2004. The most frequently isolated microorganisms were S. aureus (38.7%), P. aeruginosa (37.4%) and Haemophilus spp (15.3%). A high resistance to erithromycine (35.0%) and clindamicine (29.4%) was observed in S. aureus strains and 25.9% of them were methicillin-resistant. P. aeruginosa strains were mainly gentamicin-resistant (31.0%). The rate of ampicillin-resistant Haemophilus spp. was 23.0% and it was due to the presence of beta-lactamases, but a high trimethoprim-sulfamethoxazole resistance was observed in this microorganism (59.0%).
Assuntos
Bactérias/isolamento & purificação , Infecções Bacterianas/microbiologia , Fibrose Cística/complicações , Infecções Respiratórias/microbiologia , Infecções Bacterianas/etiologia , Criança , Pré-Escolar , Fibrose Cística/microbiologia , Suscetibilidade a Doenças , Farmacorresistência Bacteriana , Farmacorresistência Fúngica , Feminino , Fungos/efeitos dos fármacos , Fungos/isolamento & purificação , Haemophilus/efeitos dos fármacos , Haemophilus/isolamento & purificação , Infecções por Haemophilus/etiologia , Infecções por Haemophilus/microbiologia , Humanos , Lactente , Masculino , Micoses/etiologia , Micoses/microbiologia , Infecções por Pseudomonas/etiologia , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/isolamento & purificação , Infecções Respiratórias/etiologia , Infecções Estafilocócicas/etiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/isolamento & purificaçãoRESUMO
La fibrosis quística (FQ) se caracteriza por disfunciones en las glándulas de secreción exocrina del organismo. Las primeras manifestaciones suelen observarse en el sistema respiratorio, constituyendo una de las causas más importantes de morbimortalidad en los pacientes afectados. Los microorganismos patógenos que colonizan frecuentemente el tracto respiratorio de estos pacientes son Staphylococcus aureus, Haemophilus spp., y Pseudomonas aeruginosa. Entre noviembre de 2001 y agosto de 2004 se estudiaron 222 muestras respiratorias de pacientes con FQ de entre 4 meses y 11 años de edad. Se aislaron S. aureus (38,7%), P. aeruginosa (37,4%) y Haemophilus spp., (15,3%). En S. aureus la meticilina-resistencia fue del 25,9% y se asoció con altas resistencias a eritromicina (35,0%) y clindamicina (29,4%). El mayor porcentaje de resistencia observado en las cepas de P. aeruginosa fue frente a gentamicina (31,0%). Los aislamientos de Haemophilus spp. fueron resistentes a ampicilina (23,0%) debido a la presencia de beta-lactamasas, y a trimetoprima/sulfametoxazol (59,0%).
Cystic Fibrosis (CF) is characterized by a dysfunction of the exocrine secretion glands. The first symptoms often appear in the respiratory system which constitutes one of the most important morbimortality causes in these patients. Chronic respiratory tract colonization is caused mainly by bacteria such as Staphylococcus aureus, Haemophilus spp. and Pseudomonas aeruginosa. Respiratory samples from patients with CF (age group: 4 months to 11 years) were analyzed from November 2001 to August 2004. The most frequently isolated microorganisms were S. aureus (38.7%), P. aeruginosa (37.4%) and Haemophilus spp (15.3%). A high resistance to erithromycine (35.0%) and clindamicine (29.4%) was observed in S. aureus strains and 25.9% of them were methicillin-resistant. P. aeruginosa strains were mainly gentamicin-resistant (31.0%). The rate of ampicillin-resistant Haemophilus spp. was 23.0% and it was due to the presence of beta-lactamases, but a high trimethoprim-sulfamethoxazole resistance was observed in this microorganism (59.0%).
Assuntos
Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Bactérias/isolamento & purificação , Infecções Bacterianas/microbiologia , Fibrose Cística/complicações , Infecções Respiratórias/microbiologia , Infecções Bacterianas/etiologia , Fibrose Cística/microbiologia , Suscetibilidade a Doenças , Farmacorresistência Bacteriana , Farmacorresistência Fúngica , Fungos/efeitos dos fármacos , Fungos/isolamento & purificação , Infecções por Haemophilus/etiologia , Infecções por Haemophilus/microbiologia , Haemophilus/efeitos dos fármacos , Haemophilus/isolamento & purificação , Micoses/etiologia , Micoses/microbiologia , Infecções por Pseudomonas/etiologia , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/isolamento & purificação , Infecções Respiratórias/etiologia , Infecções Estafilocócicas/etiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/isolamento & purificaçãoAssuntos
Doenças dos Bovinos/epidemiologia , Surtos de Doenças/veterinária , Infecções por Haemophilus/veterinária , Haemophilus/isolamento & purificação , Meningoencefalite/veterinária , Animais , Argentina/epidemiologia , Bovinos , Feminino , Haemophilus/patogenicidade , Infecções por Haemophilus/epidemiologia , Masculino , Meningoencefalite/epidemiologiaRESUMO
Os autores descrevem e analisam um caso de fasciite necrosante cervical em lactente e revisam a literatura quanto aos aspectos diagnosticos e terapeuticos desta grave infeccao. Destacam no caso presente, a origem em infeccao comum da faringe, a extensa e rapida destruicao dos tecidos cervicais e a concomitancia da sepse. A terapeutica antibiotica dirigida ao Haemophilus sp causador do quadro, o suporte clinico em unidade de terapia intensiva pediatrica, e os repetidos debridamentos cirurgicos, foram fundamentais para a evolucao favoravel do caso
Assuntos
Humanos , Masculino , Lactente , Fasciite Necrosante/diagnóstico , Febre/etiologia , Fasciite Necrosante , Fasciite Necrosante/complicações , Fasciite Necrosante/cirurgia , Fasciite Necrosante/terapia , Seguimentos , Haemophilus/isolamento & purificação , Equipe de Assistência ao Paciente , Faringite/etiologia , Sinais e SintomasRESUMO
Mastitis caused by Prototheca spp or Nocardia spp is considered to be difficult to treat. Both microorganisms are contaminants commonly found in soil. The occurrence of mastitis caused by these agents was studied in a particular dairy farm. In this herd, the animals were kept at pasture overnight and during daytime were brought to a pen where they were fed. This pen accumulated mud and faeces, particularly in the rainy season. During milking, pre-dipping of the teats was performed with an iodide solution, but they were not washed, so a layer of soil and faeces remained which may have contaminated the milking equipment. The herd comprised 91 lactating animals and 47 dry cows. For microbiological examination, 107 milk samples were collected from lactating cows and 186 samples of mammary secretions from the dry cows. Prototheca spp were isolated from 14.55% of the milk samples and Nocardia spp from 4.55%. Prototheca spp were isolated from 8.06% of the secretion samples from dry cows and Nocardia spp were isolated from 2.15% samples. The high occurrence of mastitis due to these environmental agents reflects the problem of keeping animals in muddy pastures and pens, and the defective pre-milking hygiene for the teats.
Assuntos
Surtos de Doenças/veterinária , Mastite Bovina/epidemiologia , Mastite Bovina/microbiologia , Nocardiose/veterinária , Nocardia/isolamento & purificação , Prototheca/isolamento & purificação , Animais , Brasil , Bovinos , Corynebacterium/isolamento & purificação , Enterobacteriaceae/isolamento & purificação , Feminino , Haemophilus/isolamento & purificação , Abrigo para Animais , Nocardiose/etiologia , Staphylococcus/isolamento & purificação , Streptococcus/isolamento & purificação , Leveduras/isolamento & purificaçãoAssuntos
Galinhas , Infecções por Haemophilus/veterinária , Vacinas Anti-Haemophilus , Doenças das Aves Domésticas/diagnóstico , Doenças das Aves Domésticas/prevenção & controle , Infecções Respiratórias/veterinária , Vacinação/veterinária , Animais , Técnicas de Tipagem Bacteriana , Galinhas/imunologia , Galinhas/microbiologia , Reações Cruzadas , Haemophilus/classificação , Haemophilus/imunologia , Haemophilus/isolamento & purificação , Infecções por Haemophilus/diagnóstico , Infecções por Haemophilus/prevenção & controle , Pasteurella/classificação , Doenças das Aves Domésticas/microbiologia , Infecções Respiratórias/diagnóstico , Infecções Respiratórias/microbiologia , Infecções Respiratórias/prevenção & controle , Sorotipagem/veterinária , Especificidade da EspécieRESUMO
From March 1990 to December 1992, the National Institute for Quality Control of Health-INCQS Research Collection received 1476 bacterial samples isolated from human cerebrospinal fluid of patients suspect of meningitis in Rio de Janeiro, from the São Sebastião State Institute of Infectious Diseases (IEISS). Neisseria meningitidis was found in most of these materials, followed in smaller number by Haemophilus sp. and Streptococcus pneumoniae. The great majority of N. meningitidis strains was serogroup B, followed by serogroup C and a few strains of serogroup W135. More than 50% of the isolated bacterial agents came from the predominant 0-4 years age group. The majority of the strains were from patients in the region known as "Baixada Fluminense" (Low Lands). The aim of the work presented here is to obtain samples of meningitis cases in at least 70% of the State of Rio de Janeiro and develop a collaborative research between INCQS-FIOCRUZ and the IEISS, in order to set up a collection of strains for future studies. However, despite work being carried out in a rather satisfactory way, difficulties still arise and have to be overcome, to survey data.
Assuntos
Líquido Cefalorraquidiano/microbiologia , Meningites Bacterianas/microbiologia , Brasil , Haemophilus/isolamento & purificação , Humanos , Neisseria meningitidis/isolamento & purificação , Streptococcus pneumoniae/isolamento & purificaçãoRESUMO
Seventeen complicated outbreaks of infectious coryza in layer, broiler-breeder, and broiler flocks were studied. In the layer flock outbreaks, drops in egg production of up to 35% were seen. In the broiler flocks and several of the layer flocks, losses due to persistent mortality and/or culling varied between 2 and 5%. Signs of infectious coryza in both layers and broiler-breeders were typical; in broilers, however, swollen head-like syndrome was seen. Except in one flock, no viral diseases were clinically or serologically detected. Excluding broiler-breeders, birds from most other flocks were serologically positive for Mycoplasma gallisepticum, and some were also positive for M. synoviae. Haemophilus paragallinarum was isolated from all of the outbreaks, but only as a pure culture in three outbreaks. Isolation of H. paragallinarum from sites such as liver, kidney, and particularly tarsal arthritis and ocular globes appears to be reported for the first time. Serovar A was isolated in eight outbreaks, serovar B in six, serovar C in one, and untypable serovars in two. The severity of these infectious coryza outbreaks may have been increased by concurrent salmonellosis, pasteurellosis, and mycoplasmosis, although under certain conditions H. paragallinarum is able to cause septicemia. Ten of the outbreaks occurred in birds vaccinated against infectious coryza; this may be due to the use of vaccines that do not provide protection against the types of H. paragallinarum that affect poultry in the region.
Assuntos
Galinhas , Surtos de Doenças/veterinária , Infecções por Haemophilus/veterinária , Doenças das Aves Domésticas/epidemiologia , Animais , Argentina/epidemiologia , Surtos de Doenças/prevenção & controle , Feminino , Haemophilus/classificação , Haemophilus/isolamento & purificação , Infecções por Haemophilus/epidemiologia , Infecções por Haemophilus/prevenção & controle , Masculino , Infecções por Pasteurella/complicações , Infecções por Pasteurella/veterinária , Doenças das Aves Domésticas/prevenção & controle , Salmonelose Animal/complicações , SorotipagemRESUMO
This study was undertaken to determine the feasibility of using perioperative topical antibiotics in contaminated head and neck surgery and to standardize the culture methodology (both qualitative and quantitative) which could serve as bacteriologic endpoints for evaluation. Following preliminary studies to establish oral cavity indicator organisms and the impact of a single antibiotic mouthwash dose on oral microflora, 10 consecutive patients undergoing contaminated head and neck surgery were recruited into a clinical trial where clindamycin mouthwash and intraoperative irrigation containing clindamycin were used instead of traditional parenteral antibiotics. The bacteriologic efficacy of topical clindamycin was assessed by comparing the presence of four indicator microorganisms (two aerobic and two anaerobic) cultured from two oral cavity culture sites before and after antibiotic prophylaxis. The patients included in the study underwent total laryngectomy plus neck dissection(s) for laryngeal or hypopharyngeal carcinoma from 1991 to 1992 at a large university hospital specializing in head and neck cancer surgery. The main outcome measures used were the development of a postoperative wound infection and quantitative and qualitative bacteriology of the intraoperative neck wound and postoperative oral cavity. Two aerobic and two anaerobic organisms proved useful as a practical indicator for bacteriologic efficacy. Preoperative mouthwash resulted in a 99% reduction of both aerobic and anaerobic bacteria in intraoperatively cultured neck sites. Irrigation during surgery with the clindamycin solution further reduced the bacterial neck counts by an additional 90%. There was a consistent overgrowth of Hemophilus species on postoperative oral cavity cultures. No patient developed a postoperative wound infection. A topical prophylactic antibiotic alone was efficacious and safe for patients undergoing major contaminated head and neck surgery. Culture methods for assessment of bacteriologic efficacy were reproducible and cost-effective. This pilot study furnishes the ethical and scientific basis for large-scale prospective trials comparing topical versus parenteral antimicrobial agents.
Assuntos
Clindamicina/administração & dosagem , Laringectomia , Pré-Medicação , Administração Tópica , Idoso , Bactérias Aeróbias/isolamento & purificação , Bactérias Anaeróbias/isolamento & purificação , Carcinoma de Células Escamosas/cirurgia , Feminino , Haemophilus/isolamento & purificação , Humanos , Injeções Intravenosas , Cuidados Intraoperatórios , Neoplasias Laríngeas/cirurgia , Masculino , Pessoa de Meia-Idade , Boca/microbiologia , Antissépticos Bucais , Neoplasias Hipofisárias/cirurgiaRESUMO
O estudo de quatro anos de duracao (1987-1990) abarcou as principais caracteristicas clinico-epidemiologicas de pneumonia em criancas, tal como foi diagnosticado no servico de emergencia do Hospital Infantil, e tambem as etiologias e fatores implicados nos casos mais severos. Determinou-se a etiologia em 47.7 por cento dos 541 casos de pneumonia, que incluiam 283 patogenos, dos quais 38.6 por cento eram virus e 12.6 por cento bacterias. Observou-se etiologia viral e mista mais frequentemente nas criancas de menos de 12 meses. A etiologia prevaleceu nas idades entre 6 e 23 meses. Com relacao aos virus, a predominancia foi de RSV com prevalencia de 66 por cento do total de virus. Os mais importantes agentes bacterianos foram S. pneumoniae (64 por cento) e H. influenzae (19 por cento)....
Assuntos
Humanos , Masculino , Feminino , Recém-Nascido , Lactente , Pneumonia/epidemiologia , Haemophilus/isolamento & purificação , Infecções Respiratórias/etiologia , Pneumonia/complicações , Streptococcus pneumoniae/isolamento & purificação , UruguaiRESUMO
The biochemical and serological properties of 29 isolates of avian haemophili obtained from chickens in Brazil are described. Twenty-seven of the isolates had the typical biochemical properties of Haemophilus paragallinarum. The two remaining isolates had the typical properties of Pasteurella avium, formerly known as Haemophilus avium. All of the H. paragallinarum isolates were serotyped according to the Page scheme using a hemagglutination-inhibition test. Fourteen of the isolates were serovar A, one was serovar B, 11 were serovar C, and one isolate could not be serotyped. The isolates were also examined using a panel of monoclonal antibodies for Page serovars A (one monoclonal antibody available) and C (three monoclonal antibodies available). As expected, the serovar B isolate failed to react with any monoclonal antibody, whereas the 11 serovar C isolates reacted with all three serovar C monoclonal antibodies but not with the serovar A monoclonal antibody. Only eight of the 14 serovar A isolates reacted with the serovar A monoclonal antibody.
Assuntos
Galinhas/microbiologia , Infecções por Haemophilus/veterinária , Haemophilus/isolamento & purificação , Infecções por Pasteurella/veterinária , Pasteurella/isolamento & purificação , Doenças das Aves Domésticas/microbiologia , Animais , Brasil , Demografia , Haemophilus/classificação , Haemophilus/crescimento & desenvolvimento , Infecções por Haemophilus/microbiologia , Pasteurella/classificação , Pasteurella/crescimento & desenvolvimento , Infecções por Pasteurella/microbiologiaRESUMO
The recognition of the Brazilian purpuric fever (BPF) in 1984 led to a number of studies which showed a relation between this disease and conjunctivitis caused by Haemophilus aegyptius. The increase in cases of conjunctivitis in children associated with higher population density of eye gnats (Chloropidae: Hippelates) has been reported since last century. This phenomenon is related to the attraction that those flies show for the eyes, secretions and wounds, from where they feed on. Although there are evidences on the role of these flies in the mechanical transmission of seasonal bacterial conjunctivitis, the isolation of Haemophilus aegyptius from them in their natural habitat had not been demonstrated yet. In this study Haemophilus aegyptius associated to BPF was isolated from two pools of chloropids collected around the eyes of children with conjunctivitis which were identified as Liohippelates peruanus (Becker) and a new species Hippelates neoproboscideus.
Assuntos
Conjuntivite Bacteriana/microbiologia , Dípteros/microbiologia , Infecções por Haemophilus/transmissão , Haemophilus/isolamento & purificação , Insetos Vetores/microbiologia , Adolescente , Animais , Criança , Pré-Escolar , Humanos , LactenteRESUMO
O reconhecimento da Febre Purpurica Brasileira (FPB), em 1984, originou uma serie de estudos que revelaram uma correlacao desta doenca com conjuntivites causadas por Haemophilus aegyptius. A associacao do aumento de conjuntivites em criancas e a maior densidade populacional de cloropideos do genero Hippelates ja havia sido verificada desde o seculo passado. Este fenomeno esta relacionado ao tropismo que estes insetos apresentam pelos olhos, secrecoes e feridas de onde se alimentam....
Assuntos
Humanos , Lactente , Pré-Escolar , Criança , Adolescente , Haemophilus/isolamento & purificação , Infecções por Haemophilus/complicações , Conjuntivite Bacteriana/etiologia , Conjuntivite Bacteriana/microbiologia , Haemophilus/classificaçãoRESUMO
Se buscó la identificación de anticuerpos contra Haemophilus somnus en bovinos del municipio de Tuxtepec, Oaxaca. De enero a junio de 1989, se colectaron 300 muestras séricas de bovinos clínicamente sanos seleccionados al azar, las cuales fueron procesadas mediante la prueba de Inmunodifusión en gel, por poseer una alta especificidad ante este microorganismo. Los resultados mostraron 2 por ciento de seropositividad