RESUMO
BACKGROUND: The unpredictable behavior of giant cell lesions (GCLs) of the jaws parallels its controversial histogenesis. This study evaluated a possible association between the immunohistochemical expression of NF-ĸB, the inhibitory subunits IĸBα/IĸBß, and clinicopathological variables with the behavior of central and peripheral GCLs of the jaws. MATERIALS AND METHODS: Paraffin-embedded samples of GCLs of the jaws (n = 68) were prepared for histological/immunohistochemical assessment. Demographic and clinicopathological parameters were assessed to determine the behavior of the lesions. A staining-intensity-distribution (SID) score was used to assess the immunomarkers reactivity. The association between significant candidate immunohistochemical predictor variables regarding clinical behavior was analyzed individually and adjusted for confounding using a binary logistic regression model. RESULTS: While univariate analysis revealed a positive association of NF-ĸB SID score, NF-ĸB nuclear expression, IĸBα SID score, and NF-ĸB to inhibitors average ratio with the aggressive status of GCLs, after bivariate logistic regression analysis, only NF-ĸB nuclear expression, IĸBα SID score, and NF-ĸB to inhibitors average ratio remained as robust predictors of aggressiveness. Confounding and interaction effects regarding clinicopathological candidate predictor variables were also noted. CONCLUSION: It looks that clinical behavior of GCLs of the jaws may be strong/independently linked to the increased nuclear expression of NF-ĸB, higher NF-ĸB to inhibitors average ratio, and decreased IĸBα SID score. Notwithstanding, there are simultaneously synergistic and opposing interactive effects with respect to age stratum, growth rate, multinucleated giant cells count, and mononuclear stromal cells density in the susceptible host that may increase the tissue destruction observed in aggressive GCLs.
Assuntos
Células Gigantes/patologia , Doenças Maxilomandibulares/patologia , NF-kappa B/biossíntese , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Seguimentos , Células Gigantes/imunologia , Células Gigantes/metabolismo , Granuloma de Células Gigantes/imunologia , Granuloma de Células Gigantes/metabolismo , Granuloma de Células Gigantes/patologia , Humanos , Imuno-Histoquímica , Doenças Maxilomandibulares/imunologia , Doenças Maxilomandibulares/metabolismo , Masculino , Pessoa de Meia-Idade , NF-kappa B/imunologia , Estudos Retrospectivos , Células Estromais/imunologia , Células Estromais/metabolismo , Células Estromais/patologia , Adulto JovemRESUMO
BACKGROUND: Central giant cell lesion (CGCL) is a reactive lesion of the jaws with an associated inflammatory infiltrate. Since cell circulation allows for intense communication between different compartments in the body, we investigated whether the CGCL would lead to phenotypic and/or functional changes in circulating leukocytes. METHODS: We obtained lymphocytes and monocytes from CGCL patients and control subjects, to evaluate cytokine and adhesion molecule expression using flow cytometry. RESULTS: Our results revealed that CD4(+) T cells and CD14(+) monocytes from CGCL express elevated levels of CD11a and CD11b, respectively, when compared with controls. The frequencies of CD4(+) T cells expressing interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha and the frequencies of CD4(+) and CD14(+) cells expressing interleukin (IL)-10 were increased in CGCL group, when compared with controls. CONCLUSIONS: Our data indicate that, although CGCL is a localized lesion, the patients show systemic functional alterations in circulating leukocytes, suggesting their role in the inflammatory pathogenesis of CGCL.
Assuntos
Granuloma de Células Gigantes/imunologia , Leucócitos/imunologia , Leucócitos/metabolismo , Doenças Mandibulares/imunologia , Adulto , Moléculas de Adesão Celular/biossíntese , Citocinas/biossíntese , Feminino , Citometria de Fluxo , Imunofluorescência , Humanos , Ativação Linfocitária , Masculino , Monócitos/imunologiaRESUMO
Se estudiaron dos casos de granulomas periféricos de células gigantes, de gran tamaño, en adulto de 30 años y 50 años, comparando las características clínicas, radiográficas e histopatológicas; en este último aspecto se realizó inmunohistoquímica, utlizando el anticuerpo monoclonal MIB-1 (Ki 67 en parafina), el cual resultó de bajo índice de proliferacion celular, a pesar de no tener uno de los casos antecedente de recidiva, con un comportamiento agresivo. (AU)
Assuntos
Humanos , Adulto , Pessoa de Meia-Idade , Feminino , Granuloma de Células Gigantes/classificação , Granuloma de Células Gigantes/diagnóstico , Granuloma de Células Gigantes/patologia , Doenças da Gengiva/classificação , Anticorpos Monoclonais/análise , Imuno-Histoquímica/métodos , Recidiva , Diagnóstico Diferencial , Doenças da Gengiva , Gengiva/ultraestrutura , Granuloma de Células Gigantes/etiologia , Granuloma de Células Gigantes/imunologia , Granuloma de Células Gigantes/cirurgia , Biópsia , MicroscopiaRESUMO
Se estudiaron dos casos de granulomas periféricos de células gigantes, de gran tamaño, en adulto de 30 años y 50 años, comparando las características clínicas, radiográficas e histopatológicas; en este último aspecto se realizó inmunohistoquímica, utlizando el anticuerpo monoclonal MIB-1 (Ki 67 en parafina), el cual resultó de bajo índice de proliferacion celular, a pesar de no tener uno de los casos antecedente de recidiva, con un comportamiento agresivo.