RESUMO
The occurrence of Porphyromonas gulae, Porphyromonas macacae, Fusobacterium nucleatum and Fusobacterium canifelinum in subgingival plaque from dogs with and without periodontitis as well as their antimicrobial susceptibility were evaluated. From 50 dogs with periodontitis were identified 38 P. gulae, 8 P. macacae, 26 F. nucleatum and 15 F. canifelinum, and from 50 dogs without periodontitis were identified 15 P. gulae, 12 F. nucleatum and 11 F. canifelinum. All strains were susceptible to most of the antibiotics tested, however, different resistance rates to clarithromycin, erythromycin and metronidazole among strains were observed. The role of P. gulae, P. macacae, F. nucleatum and F. canifelinum in periodontal disease of household pets needs to be defined to a better prevention and treatment of the canine periodontitis.
Assuntos
Doenças do Cão/microbiologia , Cães/microbiologia , Fusobacterium/efeitos dos fármacos , Fusobacterium/isolamento & purificação , Periodontite/microbiologia , Porphyromonas/efeitos dos fármacos , Porphyromonas/isolamento & purificação , Animais , Antibacterianos/farmacologia , Infecções por Bacteroidaceae/microbiologia , Claritromicina/farmacologia , Placa Dentária/microbiologia , Farmacorresistência Bacteriana , Eritromicina/farmacologia , Feminino , Fusobacterium/metabolismo , Infecções por Fusobacterium/microbiologia , Testes de Inibição da Hemaglutinação , Humanos , Masculino , Metronidazol/farmacologia , Testes de Sensibilidade Microbiana , Porphyromonas/metabolismoRESUMO
Bacteriocin production has been studied in very few anaerobic bacteria, and no report is available for Fusobacterium species. In the present study a total of 167 Fusobacterium isolates were tested for bacteriocin production: 70 isolates were obtained from the oral cavity of patients with periodontal disease, 47 were recovered from healthy oral sites of human subjects and 50 from the oral cavity of Callithrix penicillata. Autoantagonism and isoantagonism were observed when the bacteriocin-producing isolates were tested against themselves. Heteroantagonism was detected by testing the Fusobacterium isolates against 14 reference strains and 2 strains of Actinobacillus actinomycetemcomitans from our laboratory collection. The auto-, iso- and heteroantagonism phenomena observed in this comparative study suggest a possible ecological role for this (these) antagonistic substance(s) in the oral environment.