RESUMO
As células-tronco mesenquimais (MSCs) são células com grande potencial de diferenciação e estão sendo recentemente introduzidas na clínica para tratamento de várias doenças. Possuem várias vantagens incluindo sua estabilidade fenotípica in vitro. OBJETIVO: isolamento das MSCs de líquido amniótico, sua expansão e a demonstração da sua capacidade de se diferenciar em células miogênicas e adipogênicas, sem alterar a estabilidade cromossomal em meio de cultura. MÉTODOS: a fim de avaliar a mudança funcional destas células, foram avaliados parâmetros bioquímicos nas células adipogênicas já diferenciadas e antes da diferenciação através da dosagem de triglicérides. A diferenciação em células musculares foi avaliada comparando os níveis de creatinofosfoquinase - CK, desidrogenase lática - LDH e aldolase produzidas por estas células antes e após diferenciação. RESULTADOS: os níveis de triglicérides foram significativamente maiores nas células diferenciadas, mostrando ainda a formação de grânulos intracitoplasmáticos. Todos os outros valores obtidos foram significativamente maiores nas células miogênicas diferenciadas quando comparadas às não diferenciadas. CONCLUSÃO: os resultados sugerem que estes protocolos podem ser usados para avaliar diferenciação de células-tronco em células adipogênicas e miogênicas, e que o líquido amniótico pode ser uma fonte para obtenção destas células.
The mesenchymals stem cells (MSCs) are cells with the great potential of differentiation are being introduced in the clinic for treatment of several diseases. Mesenchymal stem cells have several advantages including the stability of their phenotype in vitro. BACKGROUND: isolation of MSCs in amniotic fluid, its expansion and the demonstration of the capacity of these cells to differentiate in adipogenic and miogenic cells, without to change the chromosomal stability of the MSCs in culture. METHODS: in order to evaluate the functional change of these cells, were gotten values of the differentiated adipogenic cells and not differentiated through the dosage of triglycerides. The miogenic nature of the differentiated cells was analyzed comparing the creatine kinase - CK, lactic dehydrogenase - LDH and aldolase produced by the cells. RESULTS: the values of triglycerides were significantly higher in differentiated cells, showing intracitoplasmatic granule form after differentiation. All the biochemical characters were significantly higher in differentiated miogenic cells. CONCLUSIONS: this study suggests that the standardized protocol of differentiation can be used in the attainment of cells with characteristics of adipogenic and muscular cells, from amniotic fluid.
Assuntos
Feminino , Humanos , Gravidez , Células-Tronco Mesenquimais , Líquido Amniótico/citologia , Diferenciação Celular/fisiologia , Creatina Quinase/análise , Líquido Amniótico/enzimologia , Técnicas de Cultura de Células , Células Cultivadas , Frutose-Bifosfato Aldolase/análise , Cariotipagem , L-Lactato Desidrogenase/análise , Triglicerídeos/sangueRESUMO
UNLABELLED: The mesenchymals stem cells (MSCs) are cells with the great potential of differentiation are being introduced in the clinic for treatment of several diseases. Mesenchymal stem cells have several advantages including the stability of their phenotype in vitro. BACKGROUND: isolation of MSCs in amniotic fluid, its expansion and the demonstration of the capacity of these cells to differentiate in adipogenic and myogenic cells, without to change the chromosomal stability of the MSCs in culture. METHODS: in order to evaluate the functional change of these cells, were gotten values of the differentiated adipogenic cells and not differentiated through the dosage of triglycerides. The myogenic nature of the differentiated cells was analyzed comparing the creatine kinase--CK, lactic dehydrogenase--LDH and aldolase produced by the cells. RESULTS: the values of triglycerides were significantly higher in differentiated cells, showing intracytoplasmatic granule form after differentiation. All the biochemical characters were significantly higher in differentiated myogenic cells. CONCLUSIONS: this study suggests that the standardized protocol of differentiation can be used in the attainment of cells with characteristics of adipogenic and muscular cells, from amniotic fluid.
Assuntos
Líquido Amniótico/citologia , Diferenciação Celular/fisiologia , Creatina Quinase/análise , Células-Tronco Mesenquimais/citologia , Líquido Amniótico/enzimologia , Técnicas de Cultura de Células , Células Cultivadas , Feminino , Frutose-Bifosfato Aldolase/análise , Humanos , Cariotipagem , L-Lactato Desidrogenase/análise , Gravidez , Triglicerídeos/sangueRESUMO
Glucose and glutamine metabolism of two microregions of human infiltrating ductile breast cancer, the center and the periphery, was studied and the results were compared with those of healthy mammary glands. In general, the activities of glycolytic enzymes and of phosphate-dependent glutaminase were as follows: center>periphery>mammary gland. Insulin caused a marked increase of glucose consumption and lactate production by incubated slices of mammary gland but had no effect on both microregions of the tumor. Therefore, human breast cancer presents metabolic microregions.
Assuntos
Neoplasias da Mama/metabolismo , Carcinoma Ductal de Mama/metabolismo , Adolescente , Adulto , Idoso , Neoplasias da Mama/enzimologia , Neoplasias da Mama/cirurgia , Carcinoma Ductal de Mama/enzimologia , Carcinoma Ductal de Mama/cirurgia , Feminino , Frutose-Bifosfato Aldolase/análise , Glucose/metabolismo , Glucose-6-Fosfato Isomerase/análise , Glicerolfosfato Desidrogenase/análise , Humanos , Insulina/farmacologia , L-Lactato Desidrogenase/análise , Ácido Láctico/biossíntese , Pessoa de Meia-Idade , Piruvato Quinase/análise , Espectrofotometria UltravioletaRESUMO
Muscular disturbances in hypothyroid children are: generalized muscular weakness of proximal predominance, muscular hypotonicity, pseudohypertrophy (of gastrocnemius muscles chiefly), slow relaxation stage of osteotendinous reflexes, rise of muscular enzymes (CPK andaldolase), unspecific electromyographic changes and histopathologic disturbances comprised by persistent decrease of fibers type II. All these changes are reversible with thyroglobulin therapy; therefore they exert no influence on the prognosis of hypothyroid patients.