RESUMO
The skin is a critical organ for the maintenance of the integrity and protection of the organism. When a wound occurs, a sequence of healing mechanisms is triggered to reconstruct the wounded area. ß-caryophyllene is a sesquiterpene in Copaifera langsdorffii oleoresin with antioxidant and anti-inflammatory potential. On the basis of previous studies with C. langsdorffii, ß-caryophyllene was selected to evaluate its wound healing potential and pharmacological mechanisms. The excision wound model was used with male Wistar rats and macroscopic, histological, immunohistochemical and biochemical analyses were performed with skin samples, comparing the ß-caryophyllene-treated group with reference drugs. The results showed macroscopic retraction of the wounds treated with ß-caryophyllene. Biochemical assays revealed the antioxidant and anti-inflammatory mechanisms of the ß-caryophyllene-treated group with increasing levels of IL-10 and GPx and decreasing levels of pro-inflammatory molecules, including TNF-α, IFN-γ, IL-1ß and IL-6. After ß-caryophyllene treatment, immunohistochemical assays showed enhanced re-epithelialization, through the increase in laminin-γ2 and desmoglein-3 immunolabeling. ß-caryophyllene also act in the remodeling mechanism, increasing the collagen content in the Masson's trichrome staining. These findings indicated the wound-healing potential of ß-caryophyllene topical formulation in rat skin wounds, mediated by antioxidant, anti-inflammatory and re-epithelialization mechanisms.
Assuntos
Anti-Inflamatórios não Esteroides/administração & dosagem , Antioxidantes/administração & dosagem , Fabaceae/química , Compostos Fitoquímicos/administração & dosagem , Fitoterapia/métodos , Extratos Vegetais/administração & dosagem , Sesquiterpenos Policíclicos/administração & dosagem , Reepitelização/efeitos dos fármacos , Pele/lesões , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Administração Tópica , Animais , Citocinas/metabolismo , Masculino , Modelos Animais , Ratos , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos , Resultado do Tratamento , Ferimentos Penetrantes/metabolismoRESUMO
Estrogenic steroids and adenosine A2A receptors promote the wound healing and angiogenesis processes. However, so far, it is unclear whether estrogen may regulate the expression and pro-angiogenic activity of A2A receptors. Using in vivo analyses, we showed that female wild type (WT) mice have a more rapid wound healing process than female or male A2A-deficient mice (A2AKO) mice. We also found that pulmonary endothelial cells (mPEC) isolated from female WT mice showed higher expression of A2A receptor than mPEC from male WT mice. mPEC from female WT mice were more sensitive to A2A-mediated pro-angiogenic response, suggesting an ER and A2A crosstalk, which was confirmed using cells isolated from A2AKO. In those female cells, 17ß-estradiol potentiated A2A-mediated cell proliferation, an effect that was inhibited by selective antagonists of estrogen receptors (ER), ERα, and ERß. Therefore, estrogen regulates the expression and/or pro-angiogenic activity of A2A adenosine receptors, likely involving activation of ERα and ERß receptors. Sexual dimorphism in wound healing observed in the A2AKO mice process reinforces the functional crosstalk between ER and A2A receptors.
Assuntos
Estradiol/farmacologia , Receptor alfa de Estrogênio/genética , Receptor beta de Estrogênio/genética , Neovascularização Fisiológica/efeitos dos fármacos , Receptor A2A de Adenosina/genética , Ferimentos Penetrantes/genética , Adenosina/análogos & derivados , Adenosina/farmacologia , Animais , Proliferação de Células/efeitos dos fármacos , Células Endoteliais/citologia , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Receptor alfa de Estrogênio/antagonistas & inibidores , Receptor alfa de Estrogênio/metabolismo , Receptor beta de Estrogênio/antagonistas & inibidores , Receptor beta de Estrogênio/metabolismo , Feminino , Regulação da Expressão Gênica , Pulmão/citologia , Pulmão/metabolismo , Masculino , Camundongos , Camundongos Knockout , Neovascularização Fisiológica/genética , Fenetilaminas/farmacologia , Pirazóis/farmacologia , Pirimidinas/farmacologia , Receptor Cross-Talk , Receptor A2A de Adenosina/metabolismo , Fatores Sexuais , Transdução de Sinais , Cicatrização/efeitos dos fármacos , Cicatrização/genética , Ferimentos Penetrantes/tratamento farmacológico , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologiaRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Sorocea guilleminina Gaudich. is a tree or shrub endemic to Brazil. Its leaves are used in Brazilian folk medicine for the healing of wounds, stomach problems, inflammation and as diuretic. The present study evaluates the activity and action mechanisms of the healing properties of the aqueous extract of S. guilleminiana leaves (AESg), in experimental models in vivo and in vitro, as well as performs a phytochemical analysis of the extract. MATERIALS AND METHODS: The AESg was prepared by infusion: Ten g of dry leaves powder in 1â¯L hot water, soaked for 15â¯min, filtered, lyophilized, and stored at -30⯰C. Phytochemical analyses were realized by colorimetry and HPLC/ESI/MS. Its' in vitro cytotoxicity was evaluated on fibroblastic N3T3 cells. The potential of the wound healing activity in vivo was evaluated using excision and incision wound rat models, by histopathology of the injured skin along with the determination of nitric oxide, cytokines (IL-1ß, IL-10, and TNF-α), and antioxidant parameters (GSH, MPO and CAT). In vitro wound healing activity was also demonstrated in scratched N3T3 cells, by measuring the proliferation/migration rate. RESULTS: The phytochemical analysis of the AESg revealed a strong presence of polar compounds, especially flavonoids (4 majoritarian), as well as terpenes and/or sterols (2 majoritarian). The AESg showed no toxicity in the N3T3 cell line (IC50â¯>â¯800⯵g/mL). Topical treatment with the AESg showed an increase (pâ¯<â¯0.05) in wound contraction with 2â¯mg/g cream on days 5 and 9 (43.56% and 6.70% increase, respectively), and with 50â¯mg/g on days 7 and 9 (10.88% and 7.91%, respectively), compared to the vehicle (non-ionic neutral cream). Topical application of AESg (2 or 50â¯mg/g non-ionic cream) in incised wounds caused an increase in the force necessary for the rupture of the wound when compared to the vehicle group. No changes in cytokines (IL-1ß, IL-10, or TNF-α) or NO accumulation was found with up to 50â¯mg/g AESg treatment. For antioxidant activity on the incision wound, an increase in GSH levels was denoted with the AESg use, at the lowest and highest dose (2 and 50â¯mg/g) by 75.86% and 61.20% respectively, when compared to the vehicle. Also, the CAT activity was accentuated by AESg at the highest dose (50â¯mg/g) by 85.87%. Finally, the AESg at all doses attenuated MPO activity significantly in the incision wound by 71.35%, 73.21%, 78.08%, respectively. In the scratch test on N3T3 cells, the treatment with AESg resulted also in an increase in fibroblast proliferation/migration rate, compared to the vehicle. CONCLUSION: AESg is not cytotoxic. The results confirm the popular use of the leaf infusion of S. guilleminiana for the treatment of cutaneous wounds, possibly by stimulating the proliferation of fibroblasts with a consequent deposition of collagen, fastening rearrangement of collagen fibers, and greater transformation into myofibroblasts, essential in the healing process. Preliminary chemical analyzes of AESg revealed the presence mainly of phenolic compounds, being salicylic acid, gallic acid, pinocembrin and isoquercitrin the majoritarian ones.
Assuntos
Moraceae , Extratos Vegetais/farmacologia , Folhas de Planta , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Animais , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Citocinas/metabolismo , Modelos Animais de Doenças , Camundongos , Moraceae/química , Células NIH 3T3 , Óxido Nítrico/metabolismo , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/toxicidade , Folhas de Planta/química , Ratos Wistar , Reepitelização/efeitos dos fármacos , Pele/lesões , Pele/metabolismo , Pele/patologia , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologiaRESUMO
Calreticulin is an endoplasmic reticulum-resident, calcium-binding, stress-produced, chaperone protein that serves multiple functions and is widely distributed in eukaryotic cells. Exogenously applied recombinant calreticulin solution, markedly enhanced the rate and quality of skin wound healing. These modulatory effects are more efficient than commercially available topic platelet-derived growth factor ointments (Regranex®). Trypanosoma cruzi calreticulin is more effective in equimolar terms to human counterpart in accelerating skin wound healing. While the effect of externally added recombinant parasite calreticulin on wound healing has been reported, the domains responsible for these modulatory effects have not yet been established. Here, recombinant parasite calreticulin and some of its domains were tested to assess their influence in increasing proliferation and migration of fibroblasts in vitro and rat skin wound healing in vivo. Herein, we propose that Trypanosoma cruzi whole calreticulin or some of its domains are differentially involved in the modulation of wound-healing cell migration and proliferation, and cosmetic outcome. Therefore, precise combination of the parasite protein and its domains could allow us to tailor-specific desired effects during the skin wound-healing process.
Assuntos
Calreticulina/farmacologia , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fragmentos de Peptídeos/farmacologia , Proteínas de Protozoários/farmacologia , Pele/efeitos dos fármacos , Trypanosoma cruzi/metabolismo , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Animais , Calreticulina/genética , Calreticulina/metabolismo , Células Cultivadas , Modelos Animais de Doenças , Fibroblastos/metabolismo , Fibroblastos/patologia , Masculino , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/metabolismo , Domínios Proteicos , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , Ratos Sprague-Dawley , Proteínas Recombinantes/farmacologia , Pele/lesões , Pele/metabolismo , Pele/patologia , Trypanosoma cruzi/genética , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologiaRESUMO
INTRODUCCIÓN: Este documento técnico se realiza a solicitud del Hospital Nacional Docente Madre Niño San Bartolomé a través de la Gerencia Macro Regional Centro Medio del Seguro Integral de Salud. a) Cuadro clínico: Las heridas abiertas de piel se pueden dar por múltiples causas que incluyen: procedimientos quirúrgicos, úlceras, quemaduras, fasceítis necrotizante, entre otros. El propósito del manejo de las heridas es la cicatrización de éstas sin desarrollar infecciones. b) Tecnología sanitaria: Los apósitos de hidrogel con plata iónica (AHPI) tienen como principal mecanismo de acción la actividad antimicrobiana de la plata. Se ha demostrado que la plata causa la precipitación del ADN dentro de las bacterias. Además, la plata también ejerce una actividad bactericida al unirse fuertemente con las membranas y las proteínas de la pared celular bacteriana. Se considera un antibacteriano de amplio espectro, incluyendo Staphilococcus aureus metilresistente y Enterococo vancomicin resistente. OBJETIVO: Describir la evidencia científica disponible sobre la eficacia y seguridad de AHPI en el tratamiento de heridas abiertas, como pie diabético, heridas operatorias, y úlceras venosas, arteriales o de presión. METODOLOGÍA: Se realizó una búsqueda en las principales bases de datos bibliográficas: MEDLINE (PubMed), LILACS, y COCHRANE, así como en buscadores genéricos de Internet incluyendo Google Scholar y TRIPDATABASE. Adicionalmente, se hizo una búsqueda dentro de la información generada por las principales instituciones internacionales de manejo de heridas y agencias de tecnologías sanitarias que realizan revisiones sistemáticas (RS), evaluación de tecnologías sanitarias (ETS) y guías de práctica clínica (GPC). RESULTADOS: Se identificaron 3 RS, 2 ensayos clínicos aleatorizados (ECAs), 2 GPC y 1 ETS. No se identificaron evaluaciones económicas de la región. A continuación, presentaremos los resultados por tipo de herida. CONCLUSIONES: En el caso de pie diabético sólo un estudio mostró un beneficio en el promedio de tiempo de curación y granulación, y en el número de úlceras curadas con AHPI comparado con manejo estándar. Los otros dos estudios no mostraron diferencia entre AHPI y otros comparadores. En el caso de heridas operatorias, sólo se encontró ECA que evidenciaba beneficio de AHPI comparado con manejo estandar. Sin embargo, una RS de alta calidad no encuentra una diferencia entre estas comparaciones. Además, una ECA demuestra beneficios de pomada de mupirocina comparado con AHPI y no encuentra diferencia entre AHPI versus manejo de herida estándar. En ulceras venosas, no se encontró diferencias entre apósitos de plata y otros apósitos. No se encontró evidencia con para el manejo de ulceras arteriales ni úlceras de presión. Una GPC lista AHPI entre las opciones para el manejo de pie diabético, sin recomendarla por sobre otras tecnologías. Otra GPC no recomienda AHPI en el caso de úlceras venosas. Una ETS refiere que la elección se debe hacer con el paciente evaluando sus preferencias sobre costo y comodidad. Dos de las RS recibieron una calificación de nivel de confianza alto y una de ellas críticamente bajo, debido a que no presentaba una lista de los estudios excluidos con los motivos de exclusión, y no valoraba la presencia de sesgo de publicación. Los ECAs incluidos fueron valorados como de bajo y moderado riesgo de sesgo, basado en la limitación en la interpretación de los resultados y la poca muestra de los estudios.
Assuntos
Humanos , Nitrato de Prata/administração & dosagem , Ferimentos Penetrantes/tratamento farmacológico , Hidrogel de Polietilenoglicol-Dimetacrilato/administração & dosagem , Avaliação da Tecnologia Biomédica , Análise Custo-EficiênciaRESUMO
Here, we evaluate the anti-inflammatory and wound-healing effects of methanolic crude extract obtained from aerial parts (leaves and branches) of Rubus imperialis Chum. Schl. (Rosaceae) and the pure compound niga-ichigoside F1. Anti-inflammatory activity was determined in vivo and in vitro, and the healing effect was evaluated in surgical lesions in mice skin. The 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and H2O2-induced oxidative stress were used to determine antioxidant activity. The efferocytosis activity was also determined. The data obtained show that the extract of R. imperialis promote reduction in the inflammatory process induced by lipopolysaccharide (LPS) or carrageenan in the air pouch model; the effects could be reinforced by nitric oxide reduction in LPS-stimulated neutrophils, and an increase in the efferocytosis. The extract showed wound healing property in vitro and in vivo, scavenging activity for DPPH, and cytoprotection in the H2O2-induced oxidative stress in L929 cells. In addition, the compound niga-ichigoside F1 was able to reduce the NO secretion; however, it did not present wound-healing activity in vitro. Together, the data obtained point out the modulatory actions of R. imperialis extract on leukocyte migration to the inflamed tissue, the antioxidant, and the pro-resolutive activity. However, the R. imperialis anti-inflammatory activity may be mediated in parts by niga-ichigoside F1, and on wound healing do not correlated with niga-ichigoside F1.
Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Inflamação/prevenção & controle , Extratos Vegetais/farmacologia , Rubus , Saponinas/farmacologia , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/química , Carragenina , Linhagem Celular , Quimiotaxia de Leucócito/efeitos dos fármacos , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Peróxido de Hidrogênio/farmacologia , Inflamação/induzido quimicamente , Inflamação/metabolismo , Lipopolissacarídeos , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/metabolismo , Masculino , Metanol/química , Camundongos Endogâmicos BALB C , Infiltração de Neutrófilos/efeitos dos fármacos , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Fitoterapia , Picratos/química , Componentes Aéreos da Planta , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Plantas Medicinais , Rubus/química , Saponinas/química , Saponinas/isolamento & purificação , Pele/lesões , Pele/metabolismo , Pele/patologia , Solventes/química , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologiaRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Struthanthus vulgaris (Vell.) Mart. (Loranthaceae) has been largely used in traditional folk medicine in Brazil as an anti-inflammatory agent and to treat various skin disorders, including wounds. AIMS OF THE STUDY: To investigated the influence of 5% Struthanthus vulgaris ointment during cutaneous wound healing in rats. MATERIALS AND METHODS: Twenty Wistar rats were used in each group according the daily treatment, S. vulgaris 5% ointment (SV 5%) and vehicle control groups. Four full thicknesses wounds were punched in back side skin of each animal, and five animals were sacrificed after 2, 7, 14 and 21 days after surgery for histological, immunological and biochemical analysis. RESULTS: A significant wound closured area in the S. vulgaris 5% group of about 38% and 35% as compared to 19% and 21% in the control group was observed after 2 and 5 days, respectively. Histological and biochemical analysis of the skin biopsies showed that S. vulgaris treated wounds exhibited increased granulation tissue and regulated the inflammatory response by modulating the release of pro and anti-inflammatory cytokines like IL-1α, TNF-α and IL-10, nitric oxide and, growth factors like TGF-ß. Moreover, S. vulgaris showed a marked and robust increase in the deposition and organization of collagen fibers in the wounds, and improve the quality of the scar tissue. CONCLUSIONS: Altogether these data revealed that S. vulgaris seems to prevent an over expression of inflammation and accelerates wound epithelialization and might be beneficial for treating healing disorders.
Assuntos
Anti-Inflamatórios/farmacologia , Citocinas/metabolismo , Mediadores da Inflamação/metabolismo , Loranthaceae/química , Extratos Vegetais/farmacologia , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Administração Cutânea , Animais , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/isolamento & purificação , Biópsia , Cicatriz/metabolismo , Cicatriz/patologia , Modelos Animais de Doenças , Colágenos Fibrilares/metabolismo , Tecido de Granulação/efeitos dos fármacos , Tecido de Granulação/metabolismo , Tecido de Granulação/patologia , Masculino , Pomadas , Fitoterapia , Extratos Vegetais/administração & dosagem , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Plantas Medicinais , Ratos Wistar , Reepitelização/efeitos dos fármacos , Pele/lesões , Pele/metabolismo , Pele/patologia , Fatores de Tempo , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologiaRESUMO
In a previous study, we performed the chemical characterization of a polyvinyl alcohol (PVA) membrane supplemented with latex proteins (LP) displaying wound healing activity, and its efficacy as a delivery system was demonstrated. Here, we report on aspects of the mechanism underlying the performance of the PVA-latex protein biomembrane on wound healing. LP-PVA, but not PVA, induced more intense leukocyte (neutrophil) migration and mast cell degranulation during the inflammatory phase of the cicatricial process. Likewise, LP-PVA induced an increase in key markers and mediators of the inflammatory response (myeloperoxidase activity, nitric oxide, TNF, and IL-1ß). These results demonstrated that LP-PVA significantly accelerates the early phase of the inflammatory process by upregulating cytokine release. This remarkable effect improves the subsequent phases of the healing process. The polyvinyl alcohol membrane was fully absorbed as an inert support while LP was shown to be active. It is therefore concluded that the LP-PVA is a suitable bioresource for biomedical engineering.
Assuntos
Calotropis , Portadores de Fármacos , Látex/farmacologia , Membranas Artificiais , Proteínas de Plantas/farmacologia , Álcool de Polivinil/química , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Administração Cutânea , Animais , Calotropis/química , Degranulação Celular/efeitos dos fármacos , Modelos Animais de Doenças , Composição de Medicamentos , Mediadores da Inflamação/metabolismo , Interleucina-1beta/metabolismo , Látex/isolamento & purificação , Ativação de Macrófagos/efeitos dos fármacos , Mastócitos/efeitos dos fármacos , Mastócitos/metabolismo , Mastócitos/patologia , Camundongos , Infiltração de Neutrófilos/efeitos dos fármacos , Óxido Nítrico/metabolismo , Peroxidase/metabolismo , Fitoterapia , Proteínas de Plantas/isolamento & purificação , Plantas Medicinais , Pele/lesões , Pele/metabolismo , Pele/patologia , Fatores de Tempo , Fator de Necrose Tumoral alfa/metabolismo , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologiaRESUMO
Despite the large use of the Plantago major and Siparuna guianensis in traditional medicine, there are no studies demonstrating the effectiveness from extracts of these plants in the healing process by the present methodology. This study reported the effects and toxicity of the P. major and S. guianensis extracts in the wound healing compared with a commercial product used in Brazil by macroscopic and microscopic analysis. Following injury in cervical dorsal area of the mice, the extract from P. major and S. guianensis and ointment was applied after an injury in cervical dorsal area of the mice. Wound healing rates were calculated at 4, 9, 15 and 21 d after the wounding, and tissues were obtained on the ninth day for histological analysis. Moreover, mutagenic assay of extracts was performed. Mutagenicity studies carried out with plant extracts showed not mutagenic with or without metabolic activations. Reduction of the wound area occurred earlier in mice treated with P. major and control treatment. On the 15th day, the complete wound closure occurred in P. major-treated wounds. Throughout ointment and S. guianensis treatment it was not observed the wound closured. Microscopic analyses of the wound, on the ninth day, showed the more efficient formation of the neoepithelium and skin appendages in animals treated with S. guianensis and P. major, while ointment treatment presented no re-epithelialization and absent skin appendages in wound. Thus, P. major extract showed good effects on wound healing processes rendering it a promising candidate for the treatment of wounds what also justified its traditional usage in wound treatment.
Assuntos
Extratos Vegetais , Plantago/química , Salmonella typhimurium/crescimento & desenvolvimento , Salmonella typhimurium/genética , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Animais , Masculino , Camundongos , Testes de Mutagenicidade/métodos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Ferimentos Penetrantes/genética , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologiaRESUMO
Wound healing is divided into three phases: inflammatory, proliferative and remodeling. Mast cells participate in all these phases. The aim of the present study was to determine the effects of propolis on the population of mast cells in oral surgical wounds in comparison to the results obtained with dexamethasone. This study was prospective, in vivo, randomized, semiexperimental, quantitative and comparative animal. A circular surgical wound was made on the dorsum of the tongue of 90 hamsters divided into three experimental groups: topical application of 30% propolis alcoholic extract (Group 1); 0.1% dexamethasone in orabase cream (Group 2); and orabase cream alone (Group 3). Applications were performed every 12 h throughout the experiment. The postoperative times for killing of the animals were 1, 3, 7, 14 and 28 days. The Student's t test for independent samples was employed in the statistical analysis. In the inflammatory phase of healing, propolis caused a greater reduction in the number of mast cells on the edge and in the central region of the surgical wound in comparison to dexamethasone. Moreover, the number of mast cells on day 1 was lower in the central region of the wounds treated with the orabase cream alone in comparison to dexamethasone. In conclusion, the anti-inflammatory action of propolis mediated by mast cells was more effective than dexamethasone in the inflammatory phase of healing.
Assuntos
Mastócitos/efeitos dos fármacos , Própole/uso terapêutico , Língua/lesões , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Administração Tópica , Animais , Cricetinae , Dexametasona/administração & dosagem , Dexametasona/uso terapêutico , Modelos Animais de Doenças , Feminino , Flavonoides/análise , Masculino , Mastócitos/imunologia , Mastócitos/patologia , Polifenóis/análise , Própole/administração & dosagem , Própole/química , Distribuição Aleatória , Língua/patologia , Ferimentos Penetrantes/imunologia , Ferimentos Penetrantes/patologiaAssuntos
Infecções Comunitárias Adquiridas/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Traumatismos da Mão/microbiologia , Infecções dos Tecidos Moles/microbiologia , Stenotrophomonas maltophilia/isolamento & purificação , Infecção dos Ferimentos/microbiologia , Ferimentos Penetrantes/microbiologia , Acidentes por Quedas , Adulto , Antibacterianos/uso terapêutico , Argentina/etnologia , Infecções Comunitárias Adquiridas/tratamento farmacológico , Farmacorresistência Bacteriana Múltipla , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , Imunocompetência , Masculino , Infecções dos Tecidos Moles/tratamento farmacológico , Espanha , Stenotrophomonas maltophilia/efeitos dos fármacos , Combinação Trimetoprima e Sulfametoxazol/uso terapêutico , Infecção dos Ferimentos/tratamento farmacológico , Ferimentos Penetrantes/tratamento farmacológicoRESUMO
The purpose of this study was to carry out a systematic review of the literature regarding the use of topic iodine and/or compounds in the treatment of acute wounds. The clinical trials were searched at the Cochrane's database using the following descriptors: iodine, acute wound, treatment, healing, infection, surgery, surgical. Twenty studies (52.6%) out of 38 met the criteria for inclusion. The articles were analyzed regarding journal and study characteristics and classified into five groups: iodine versus other topic agents (4/20%); iodine versus types of dressings (1/5%); iodine versus saline solution (5/25%); iodine versus no iodine (8/40%); and iodine at different concentrations (2/10%). Favorable results for the use of iodine and/or compounds occurred in 45% of the studies. Three out of 5 studies showed favorable results for healing and prevention of infection and 9 out of 15 studies were not favorable when only infection prevention was investigated.
Assuntos
Compostos de Iodo/administração & dosagem , Ferimentos Penetrantes/tratamento farmacológico , Administração Tópica , HumanosRESUMO
Esta pesquisa objetivou realizar revisão sistemática da literatura relacionada ao uso de iodóforos tópicos no tratamento de feridas agudas. Os ensaios clínicos foram localizados por meio da Base de Dados Cochrane, utilizando-se os seguintes descritores: iodine, acute wound, treatment, healing, infection, surgery, surgical. Vinte (52,6 por cento), dentre 38 artigos, enquadraramse nos critérios de inclusão, sendo analisados quanto às características dos periódicos e dos estudos e classificados em: iodóforo versus outros agentes tópicos (4/ 20 por cento); iodóforo versus coberturas (1/ 5 por cento); iodóforo versus soro fisiológico (5/ 25 por cento); iodóforo versus sem iodóforo (8/ 40 por cento) e iodóforos em diferentes concentrações (2/ 10 por cento). Resultados favoráveis para os iodóforos ocorreram em 45 por cento dos artigos. Quanto às tendências dos resultados, três dentre cinco artigos mostraram-se favoráveis para a cicatrização de feridas e prevenção de infecção e nove dentre 15 artigos foram desfavoráveis ao uso de iodóforos tópicos quando somente a prevenção de infecção foi investigada.
The purpose of this study was to carry out a systematic review of the literature regarding the use of topic iodine and/or compounds in the treatment of acute wounds. The clinical trials were searched at the Cochrane's database using the following descriptors: iodine, acute wound, treatment, healing, infection, surgery, surgical. Twenty studies (52.6 percent) out of 38 met the criteria for inclusion. The articles were analyzed regarding journal and study characteristics and classified into five groups: iodine versus other topic agents (4/ 20 percent); iodine versus types of dressings (1/ 5 percent); iodine versus saline solution (5/ 25 percent); iodine versus no iodine (8/ 40 percent); and iodine at different concentrations (2/ 10 percent). Favorable results for the use of iodine and/or compounds occurred in 45 percent of the studies. Three out of 5 studies showed favorable results for healing and prevention of infection and 9 out of 15 studies were not favorable when only infection prevention was investigated.
En esta investigación se tuvo como objetivo realizar una revisión sistemática de la literatura respecto a la utilización del yodo tópico y/o compuestos en el tratamiento de las heridas agudas. Los ensayos clínicos fueron localizados por medio de la Base de Datos Cochrane, utilizándose los siguientes descriptores: iodine, acute wound, treatment, healing, infection, surgery, surgical. Veinte (52,6 por ciento), de 38 artículos, se encuadraron en los criterios de inclusión, siendo analizados en cuanto a las características de los periódicos y de los estudios y clasificados en: yodo versus otros agentes tópicos (4/ 20 por ciento); yodo versus apósitos (1/ 5 por ciento); yodo versus solución fisiológica (5/ 25 por ciento); yodo versus sin yodo (8/ 40 por ciento) y yodo en diferentes concentraciones (2/ 10 por ciento). Resultados favorables para el yodo se obtuvieron en el 45 por ciento de los artículos. En cuanto a las tendencias de los resultados, tres de cinco artículos se mostraron favorables para la cicatrización de heridas y prevención de infección y nueve de 15 artículos fueron desfavorables al uso del yodo tópico cuando sólo la prevención de infección fue investigada.
Assuntos
Humanos , Compostos de Iodo/administração & dosagem , Ferimentos Penetrantes/tratamento farmacológico , Administração TópicaRESUMO
This study assessed the ability of Portland cement, white Angelus-mineral trioxide aggregate (MTA), and MTA Bio to seal furcal perforations in extracted human molar teeth. Fifty-five human mandibular molar teeth were accessed, and the canal orifices were located. The roots were horizontally sectioned in the middle third. Resin composite was used to fill the root canal orifices and the apical end of the root. Perforations were created in the center of the pulp chamber floor by using a size 3 round bur. The teeth were divided into 3 groups (n = 15), and an additional 10 teeth served as controls. In G1, the perforation defects were repaired with MTA, whereas in G2 and G3, MTA Bio and Portland cement were used, respectively. Each tooth was assembled in a hermetic cell to allow the evaluation of fluid filtration. Leakage was measured by the movement of an air bubble traveling within a pipette connected to the teeth. Measurements of the air bubble movement were made after 10 minutes at a constant pressure of 20 cm H(2)O. Kruskal-Wallis H test was applied to the fluid flow data to detect differences between the experimental groups (P < .05). Leakage existed in every sample and was very variable in all the experimental groups, ranging from 0.098-0.51 microL/min. Kruskal-Wallis H-test results showed that there was no significant difference in mean fluid flow between the experimental groups (P = .874). The sealing ability promoted by the 3 cements was similar; no cement was able to produce a fluid-tight seal.
Assuntos
Infiltração Dentária/prevenção & controle , Materiais Restauradores do Canal Radicular/uso terapêutico , Traumatismos Dentários/tratamento farmacológico , Raiz Dentária/lesões , Compostos de Alumínio/uso terapêutico , Compostos de Cálcio/uso terapêutico , Cimentos Dentários/uso terapêutico , Instrumentos Odontológicos/efeitos adversos , Combinação de Medicamentos , Humanos , Dente Molar/lesões , Óxidos/uso terapêutico , Silicatos/uso terapêutico , Estatísticas não Paramétricas , Traumatismos Dentários/etiologia , Ferimentos Penetrantes/tratamento farmacológico , Ferimentos Penetrantes/etiologiaRESUMO
Rats treated with 100 mg /kg/day of the Catharanthus roseus ethanol extract had high rate of wound contraction significantly decreased epithelization period, significant increase in dry weight and hydroxyproline content of the granulation tissue when compared with the controls. Wound contraction together with increased tensile strength and hydroxyproline content support the use of C. roseus in the management of wound healing.
Assuntos
Catharanthus , Fitoterapia , Extratos Vegetais/farmacologia , Cicatrização/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Tecido de Granulação/efeitos dos fármacos , Masculino , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Folhas de Planta , Ratos , Ratos Sprague-Dawley , Ferimentos Penetrantes/tratamento farmacológicoRESUMO
Las primeras descripciones de una lesión cardiaca se remontan al papiro de Edwin Smith alrededor del 3000 AC. Hasta el siglo IX, las heridas penetrantes cardiacas eran consideradas intratables y mortales. Fue en 1896, cuando se reportó la primera reparación cardiaca exitosa. Aunque la mortalidad ha disminuido con el paso del tiempo, una herida penetrante al corazón sigue teniendo un grave pronóstico y es causa importante de morbilidad y mortalidad en pacientes de trauma. En la actualidad, cada vez se ven con más frecuencia las heridas penetrantes cardiacas por arma de fuego, lo que indudablemente ensombrece aún más el pronóstico de estas lesiones, por lo que se torna de vital importancia para el cirujano que trabaja en una Unidad de Emergencia, conocer con exactitud los mecanismos fisiopatológicos que se ven involucrados en este tipo de situaciones, además de todas las complicaciones que pueden ocurrir al intentar reparar una herida penetrante cardiaca. Este artículo pretende dar una visión precisa, clara y actual del manejo de un paciente con una herida penetrante cardiaca.
Assuntos
Humanos , Ferimentos Penetrantes/cirurgia , Traumatismos Cardíacos/cirurgia , Traumatismos Cardíacos/etiologia , Adenosina/administração & dosagem , Antiarrítmicos/administração & dosagem , Emergências , Ferimentos Penetrantes/fisiopatologia , Ferimentos Penetrantes/tratamento farmacológico , Traumatismos Cardíacos/fisiopatologia , Traumatismos Cardíacos/tratamento farmacológico , Complicações Pós-Operatórias , Ressuscitação , Toracotomia , Tamponamento Cardíaco/cirurgia , Tamponamento Cardíaco/etiologiaRESUMO
Acalypha langinia is a well-known plant in the traditional medicine. Based on its traditional use, this plant was selected for evaluation of its wound healing potential. Topical application twice a day for 7 days of 0.05%, 0.1%, 0.2%, 0.4% and 0.5% sterile solution of aqueous extract from leaves of A. langinia significantly increased the healing process.
Assuntos
Diabetes Mellitus Experimental , Euphorbiaceae , Fitoterapia , Extratos Vegetais/farmacologia , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Administração Cutânea , Animais , Complicações do Diabetes/tratamento farmacológico , Complicações do Diabetes/fisiopatologia , Relação Dose-Resposta a Droga , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Folhas de Planta , Ratos , Ratos Wistar , Pele/lesões , Estreptozocina , Resistência à Tração , Ferimentos Penetrantes/tratamento farmacológico , Ferimentos Penetrantes/fisiopatologiaRESUMO
BACKGROUND: The abdomen ranks in third place of body areas injured by trauma. Evaluation and stabilization of these patients form the cornerstone in emergency medicine. Diagnostic approach and treatment outcome are influenced by several factors. Injury mechanism and pattern vary according to geography and there is an association with drugs and alcohol. Physical examination remains the most reliable indicator for surgery. Associated injuries are present in up to 26% of cases. We undertook this study to determine penetrating abdominal trauma incidence and frequency, demographic factors, rate of immediate and delayed laparotomies, and associated complications as well as to define the usefulness of penetrating abdominal trauma index (PATI). METHODS: An observational, prospective, longitudinal descriptive study was carried out at the Hospital Central "Ignacio Morones Prieto," San Luis Potosi, Mexico from January 1, 2005 to December 31, 2005 on patients who underwent exploratory laparotomy for penetrating abdominal trauma. Twenty one variables were studied. Basic statistical analysis, ANOVA, chi(2) and Student's t-test were used. RESULTS: Of the 79 patients who were included, 93.67% were males. The third decade of life was the most affected, with a night presentation being predominant as a result of personal violence. Drug use was observed in 50.6%; stab wounds in 63.3%. The most frequent locations were the left upper and right lower quadrants and epigastrium; solitary wounds were predominant. Associated injuries were most common in the thorax and limbs. Of the laparotomies performed, 92.4% were urgent and 60.53% were therapeutic; 15.19% required reoperations; complications were observed in 39.24%; and mortality rate was 3.9%. CONCLUSIONS: Due to high non-therapeutic and negative laparotomies rates, a more selective approach is needed, including repetitive physical examination and the appropriate use of auxiliary diagnostic studies.
Assuntos
Traumatismos Abdominais/epidemiologia , Laparotomia/estatística & dados numéricos , Ferimentos Penetrantes/epidemiologia , Traumatismos Abdominais/diagnóstico , Traumatismos Abdominais/tratamento farmacológico , Traumatismos Abdominais/cirurgia , Adulto , Alcoolismo/epidemiologia , Antibacterianos/uso terapêutico , Estudos de Coortes , Terapia Combinada , Comorbidade , Emergências , Feminino , Humanos , Laparotomia/métodos , Masculino , México/epidemiologia , Complicações Pós-Operatórias/epidemiologia , Estudos Prospectivos , Reoperação , Transtornos Relacionados ao Uso de Substâncias/epidemiologia , Fatores de Tempo , Procedimentos Desnecessários , Violência , Vísceras/lesões , Vísceras/cirurgia , Ferimentos por Arma de Fogo/diagnóstico , Ferimentos por Arma de Fogo/tratamento farmacológico , Ferimentos por Arma de Fogo/epidemiologia , Ferimentos por Arma de Fogo/cirurgia , Ferimentos Penetrantes/diagnóstico , Ferimentos Penetrantes/tratamento farmacológico , Ferimentos Penetrantes/cirurgia , Ferimentos Perfurantes/diagnóstico , Ferimentos Perfurantes/tratamento farmacológico , Ferimentos Perfurantes/epidemiologia , Ferimentos Perfurantes/cirurgiaRESUMO
Growth factors have proved to be an effective therapeutic strategy. However, some controversies have arisen concerning their efficacy in topical wound treatments. Stabilization of epidermal growth factors at the wound site and long-lasting receptor occupancy are important factors for wound repair. This study evaluated the cumulative profiles of two jellies containing 10 or 20 microg of 125I-rhEGF per gram of jelly, in a rat full-thickness skin lesion model. The prolonged time-courses at the wound sites for both strengths compared with saline solutions previously evaluated using a similar skin lesion model are reported. It seems that these two topical formulations that provide more sustained amounts of 125I-rhEGF over the period of sampling, would probably achieve the required wound healing response in terms of cell proliferation, collagen deposition and protein synthesis. Further studies need to be developed in order to elucidate whether such an in vivo disposition pattern is consistent with an earlier and stronger promotion of wound healing events.
Assuntos
Fator de Crescimento Epidérmico/administração & dosagem , Fator de Crescimento Epidérmico/farmacocinética , Ferimentos Penetrantes/tratamento farmacológico , Ferimentos Penetrantes/metabolismo , Administração Tópica , Animais , Química Farmacêutica , Fator de Crescimento Epidérmico/uso terapêutico , Géis , Humanos , Radioisótopos do Iodo/administração & dosagem , Radioisótopos do Iodo/farmacocinética , Masculino , Ratos , Ratos Sprague-Dawley , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/farmacocinética , Proteínas Recombinantes/uso terapêuticoRESUMO
Hamelia patens Jacq. (Rubiaceae) has received little attention in the laboratory for its wound healing ability even though it is commonly used as a treatment for wounds throughout Central America. A double incision wound healing bioassay was carried out with a crude extract of Hamelia patens collected from El Salvador. Animals were divided into three groups. Group I (n = 14) had the left incision treated with 5% (w/w) Hamelia patens and the contralateral side with petroleum jelly (PJ). Group II (n = 14) had the left incision treated with 10% (w/w) ointment and the contralateral side with petroleum jelly. Group III (n = 10) had the left incision treated with petroleum jelly and the contralateral side left untreated. Breaking strength of the incisions was measured on day 7 and day 12. For Groups I and II, there was no significant difference between treatment and control incisions at day 7. On day 12, there was a significant difference between the treated and control incisions for Groups I and II. There was no significant difference between petroleum jelly and untreated incisions for Group III on day 7 and day 12. Hamelia patens does increase breaking strength of wounds significantly more than the control group. Further wound healing studies of this plant are warranted.