RESUMO
PURPOSE: To assess the surgical wound (SW) healing in orthopedic patients with Impaired tissue integrity according to the Nursing Outcomes Classification (NOC). METHODS: A prospective longitudinal study performed with 24 patients. Data collection was performed through an instrument containing the NOC indicators. FINDINGS: The indicators skin approximation, drainage, surrounding skin erythema, periwound edema, increased skin temperature, and foul wound odor presented a statistically significant increase when compared the means between the first and the last day of evaluation. CONCLUSION: The NOC indicators allowed to monitor the SW healing indicating an improvement. IMPLICATIONS FOR NURSING PRACTICE: The NOC can favor an earlier identification of the degree of commitment for each patient and enables implementation of care to achieve target outcomes.
Assuntos
Ortopedia , Avaliação de Resultados em Cuidados de Saúde , Ferida Cirúrgica/enfermagem , Cicatrização , Adulto , Idoso , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Ferida Cirúrgica/fisiopatologiaRESUMO
BACKGROUND: Chronic kidney disease affects more than 500 million people worldwide. In this context, the uremic toxins present are related to worsening in tissue healing. AIM: Evaluate on healing of colonic anastomosis in uremic rats, serum and anatomopathological indicators, which may be related to the change tissue repair process. METHODS: Twenty Wistar rats, were randomly separated into two groups. In the sham group they were submitted to 5/6 nephrectomy simulation in left kidney, simulation right nephrectomy, median laparotomy, colotomy and colorraphy. In the uremia group, they were submitted to 5/6 nephrectomy of the left kidney, total nephrectomy of the right kidney and median laparotomy, colotomy and colorraphy. Were collected for serum urea, creatinine and CRP dosages and the colonic segments were studied for evaluation of granulation tissue, collagen maturation, microvascular and myofibroblasts density, and cell viability. Through histochemical processing, microvascular density was evaluated by anti-CD34 monoclonal antibody marking, cell viability by cell proliferation nuclear antigen screening and myofibroblasts density with monoclonal anti-α-actin antibody. Computerized histometry was used for evaluations of collagens type I and III by the coloration of picrosirius. RESULTS: The group submitted to nephrectomy 5/6, compared to the sham group, show urea increase (p<0.0000) and higher C reactive protein (p=0.0142). Decrease of granulation tissue formation (border reepithelialization p=0,0196, angiofibroblast proliferation p=0.0379), mean collagen I (p=0,0009) and collagen III (p=0,016), microvascular density (p=0,0074), cell proliferation nuclear antigen (p<0,0000) and myofibroblasts (p<0,0001). CONCLUSION: The uremia induced by nephrectomy 5/6 model establishes negative impact in the colonic wound healing.
Assuntos
Colo/cirurgia , Ferida Cirúrgica/fisiopatologia , Uremia/fisiopatologia , Cicatrização/fisiologia , Anastomose Cirúrgica , Animais , Proteína C-Reativa/análise , Colágeno Tipo I/análise , Colágeno Tipo I/metabolismo , Colágeno Tipo III/análise , Colágeno Tipo III/metabolismo , Tecido de Granulação/fisiopatologia , Miofibroblastos/fisiologia , Nefrectomia , Distribuição Aleatória , Ratos Wistar , Insuficiência Renal Crônica/fisiopatologiaRESUMO
PURPOSE: To compare wound healing performed with cold blade (CSB) and ultrasonic harmonic scalpel (UHS) in the abdominal aponeurosis of rats. METHODS: Eighty Wistar rats divided into two groups and underwent midline incision in the linea alba with cold blade and harmonic ultrasonic scalpel. Analysis were performed in subgroups of 10 animals after 3, 7, 14 and 21 days. Macroscopically was observed the presence of hematoma, infection, wound dehiscence, fistula and adherences. Microscopically were used collagen and immunohistochemical staining methods. RESULTS: Macroscopic, complications showed no statistical difference. Immunohistochemical analysis for MMP-9 was more intense in UHS group (p<0.05). TGF ß presented its lower expression in UHS group at 14 and 21 days, with no statistical difference at 3 and 7 days (p<0.05). α-AML expression appeared higher in UHS group after 14 days and remained similar in others (p<0.05). Collagen deposition had no change in type I, and increased in type III in UHS; at 7th day the deposition was higher in CSB group; at 14th was similar in both groups (p<0.001). CONCLUSION: UHS compared to the CSB has higher lesion area at the time of the incision; as well as it led to the delay of regeneration and scar maturation process.
Assuntos
Parede Abdominal/cirurgia , Colágeno/fisiologia , Ferida Cirúrgica/patologia , Cicatrização/fisiologia , Parede Abdominal/patologia , Animais , Imuno-Histoquímica , Masculino , Modelos Animais , Ratos , Ratos Wistar , Instrumentos Cirúrgicos , Ferida Cirúrgica/fisiopatologia , Análise Serial de Tecidos , Procedimentos Cirúrgicos UltrassônicosRESUMO
Abstract Purpose: To compare wound healing performed with cold blade (CSB) and ultrasonic harmonic scalpel (UHS) in the abdominal aponeurosis of rats. Methods: Eighty Wistar rats divided into two groups and underwent midline incision in the linea alba with cold blade and harmonic ultrasonic scalpel. Analysis were performed in subgroups of 10 animals after 3, 7, 14 and 21 days. Macroscopically was observed the presence of hematoma, infection, wound dehiscence, fistula and adherences. Microscopically were used collagen and immunohistochemical staining methods. Results: Macroscopic, complications showed no statistical difference. Immunohistochemical analysis for MMP-9 was more intense in UHS group (p<0.05). TGF β presented its lower expression in UHS group at 14 and 21 days, with no statistical difference at 3 and 7 days (p<0.05). α-AML expression appeared higher in UHS group after 14 days and remained similar in others (p<0.05). Collagen deposition had no change in type I, and increased in type III in UHS; at 7th day the deposition was higher in CSB group; at 14th was similar in both groups (p<0.001). Conclusion: UHS compared to the CSB has higher lesion area at the time of the incision; as well as it led to the delay of regeneration and scar maturation process.
Assuntos
Animais , Masculino , Ratos , Cicatrização/fisiologia , Colágeno/fisiologia , Parede Abdominal/cirurgia , Ferida Cirúrgica/patologia , Instrumentos Cirúrgicos , Imuno-Histoquímica , Ratos Wistar , Modelos Animais , Parede Abdominal/patologia , Análise Serial de Tecidos , Procedimentos Cirúrgicos Ultrassônicos , Ferida Cirúrgica/fisiopatologiaRESUMO
ABSTRACT Background: Chronic kidney disease affects more than 500 million people worldwide. In this context, the uremic toxins present are related to worsening in tissue healing. Aim: Evaluate on healing of colonic anastomosis in uremic rats, serum and anatomopathological indicators, which may be related to the change tissue repair process. Methods: Twenty Wistar rats, were randomly separated into two groups. In the sham group they were submitted to 5/6 nephrectomy simulation in left kidney, simulation right nephrectomy, median laparotomy, colotomy and colorraphy. In the uremia group, they were submitted to 5/6 nephrectomy of the left kidney, total nephrectomy of the right kidney and median laparotomy, colotomy and colorraphy. Were collected for serum urea, creatinine and CRP dosages and the colonic segments were studied for evaluation of granulation tissue, collagen maturation, microvascular and myofibroblasts density, and cell viability. Through histochemical processing, microvascular density was evaluated by anti-CD34 monoclonal antibody marking, cell viability by cell proliferation nuclear antigen screening and myofibroblasts density with monoclonal anti-α-actin antibody. Computerized histometry was used for evaluations of collagens type I and III by the coloration of picrosirius. Results: The group submitted to nephrectomy 5/6, compared to the sham group, show urea increase (p<0.0000) and higher C reactive protein (p=0.0142). Decrease of granulation tissue formation (border reepithelialization p=0,0196, angiofibroblast proliferation p=0.0379), mean collagen I (p=0,0009) and collagen III (p=0,016), microvascular density (p=0,0074), cell proliferation nuclear antigen (p<0,0000) and myofibroblasts (p<0,0001). Conclusion: The uremia induced by nephrectomy 5/6 model establishes negative impact in the colonic wound healing.
RESUMO Racional: A doença renal crônica atinge mais de 500 milhões de pessoas em todo o mundo. Neste contexto, as toxinas urêmicas estão relacionadas ao comprometimento da cicatrização tecidual. Objetivo: Avaliar, na cicatrização de anastomoses colônicas de ratos urêmicos indicadores séricos e anatomopatológicos que possam estar relacionados com alteração do processo de reparação tissular. Métodos: Utilizaram-se 20 ratos Wistar divididos aleatoriamente em dois grupos. No grupo simulação eles foram submetidos à simulação da nefrectomia 5/6 do rim esquerdo, simulação de nefrectomia total do rim direito, laparotomia mediana, colotomia e colorrafia. No grupo uremia, eles foram submetidos à nefrectomia 5/6 do rim esquerdo, nefrectomia total do rim direito, laparotomia mediana, colotomia e colorrafia. Coletaram-se amostras de sangue para dosagens séricas da ureia, creatinina e proteína C reativa, e do cólon para processamentos histológicos e histoquímicos na avaliação do tecido de granulação, maturação de colágeno, densidade microvascular e de miofibroblastos, viabilidade celular cicatricial. Empregou-se a histometria computadorizada para as avaliações de colágenos tipos I e III, densidade microvascular pela marcação com anticorpo monoclonal anti-CD34, viabilidade celular pela pesquisa do antígeno nuclear de proliferação celular e a densidade de miofibroblastos com anticorpo monoclonal anti-α-actina. Resultados: O grupo submetido à nefrectomia 5/6, em comparação ao grupo simulação, demonstraram aumentos da ureia sérica (p<0,0000) e proteína C reativa (p=0,0142), redução da formação de tecido de granulação (reepitelização de bordas p=0,0196, proliferação angiofibroblástica p=0,0379), porcentagens de colágeno I (p=0,0009) e colágeno III (p=0,016), densidade microvascular (p=0,0074) e miofibroblastos (p<0,0001) e antígeno nuclear de proliferação celular (p<0,0000). Conclusão: A uremia induzida pelo modelo de nefrectomia 5/6 determina impacto negativo no processo de cicatrização colônico.
Assuntos
Animais , Uremia/fisiopatologia , Cicatrização/fisiologia , Colo/cirurgia , Ferida Cirúrgica/fisiopatologia , Proteína C-Reativa/análise , Anastomose Cirúrgica , Distribuição Aleatória , Ratos Wistar , Colágeno Tipo I/análise , Colágeno Tipo I/metabolismo , Colágeno Tipo III/análise , Colágeno Tipo III/metabolismo , Insuficiência Renal Crônica/fisiopatologia , Miofibroblastos/fisiologia , Tecido de Granulação/fisiopatologia , NefrectomiaRESUMO
A terapia com laser de baixa potencia se tornou algo de inúmeras pesquisas,devido a melhora do processo cicatricial.Um estudo experimental foi realizado para avaliar se há benefícios dessa terapia na cicatrização de feridas na pele de ratos com análises não pareadas e pareadas. Foram utilizados 20 ratos Wistar dividos em 4 grupos, submetidos a 4 incisões lineares na pele do dorso.No Grupo 1, usamos uma dose de laser no pós-operatório; no Grupo 2, duas doses, uma no pós-operatório e após 24 horas; no Grupo 3, três doses, no pós-operatório , 24 e 48 horas.O grupo controle foi submetido às incisões da pele.Foram testadas a força de ruptura e analisados os achados histopatológicos das incisões.Em análise não pareada, o grupo 1 apresenta o maior valor, tendo diferença (p = 0,040). A força de ruptura dos quatro grupos de ratos para as feridas não tratadas, não ocorreu. Na análise pareada as feridas tratadas com laser nos grupos 1, 2 e 3 e grupo controle, não houve diferença. Em análise não pareada, segundo a epitelização e a granulação, o grupo 3 apresentou maior freqüência para epitelização (p=0,002) e granulação (p=0,012).Nas feridas não tratadas nos quatro grupos não houve diferença entre elas em relação à epitelização e à granulação. Na análise pareada, não ocorreram diferenças dessas para os ferimentos produzidos nos grupos.É improvável existir relação entre a terapia a laser e a cicatrização, sendo a associação na análise não pareada apenas aparente, justificada pelo método de avaliação .(AU)
Low power laser therapy is recently in focus due to its properties in angiogenesis, fibroblast and colagenase activity. Based on these facts, the benefits of low laser power therapy in wound healing have been considered. In order to evaluate the benefits of low power therapy in wound healing, twenty Wistar rats were divided into four groups (n=5) which received four linear incisions in the back and then the following treatments, 1: a post-incision laser dose 2: one post-incision and 24 hours later dose 3: one post-incision, 24 and 48 hours later laser doses. 4: control group, with the four skin incisions and no laser therapy. Rupture force and histopathological analyses were performed. Unpaired analyses showed that epithelialization (p= 0.002) and granulation (p=0.012) were more frequent. But there were no differences between groups, using paired analyses. Thus, it is not likely that there is a correlation between low power laser treatment and wound healing. Our findings using unpaired analyses can be justified by the findings using unpaired analyses can be justified by the method of analyses.(AU)