RESUMO
We describe two novel fungi isolated from soil samples collected in Northern Argentina and belonging to the family Aspergillaceae of the order Eurotiales: Leiothecium cristatum sp. nov. and Aspergillus posadasensis sp. nov. Leiothecium cristatum sp. nov., represented by the ex-type strain FMR 11998(T) (â=âCBS 134260(T)â=âNBRC 109843(T)), is distinguishable morphologically from the type species of the genus, Leiothecium ellipsoideum, by the presence of irregular reticulate ascospores with two prominent equatorial crests, and Aspergillus posadasensis sp. nov., represented by the ex-type strain FMR 12168(T) (â=âCBS 134259(T)â=âNBRC 109845(T)), is differentiated from Aspergillus acanthosporus, the nearest species phylogenetically, by its non-sclerotioid ascomata and a lack of an asexual stage on all culture media tested. The taxonomic proposals are supported by the analysis of the sequences of the internal transcribed spacer region, the D1-D2 domains of the 28S rRNA gene, the fragments of the RNA polymerase II largest subunit, and the putative chaperonin complex related to TCP-1, ß-tubulin and calmodulin genes.
Assuntos
Eurotiales/classificação , Filogenia , Microbiologia do Solo , Argentina , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Eurotiales/genética , Eurotiales/isolamento & purificação , Genes Fúngicos , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , RNA Ribossômico 28S/genética , Análise de Sequência de DNARESUMO
Elaphomyces compleximurus sp. nov. and E. digitatus sp. nov. are described from the Pakaraima Mountains of Guyana. Macromorphological, micromorphological, habitat and DNA sequence data are provided for each new species. This is the first report of Elaphomyces ascomata associated with ectomycorrhizal members of the Fabaceae and also for the genus from the lowland South American tropics.
Assuntos
Eurotiales/classificação , Micorrizas/classificação , DNA Fúngico/genética , DNA Ribossômico/genética , Ecossistema , Eurotiales/genética , Eurotiales/ultraestrutura , Guiana , Micorrizas/genética , Micorrizas/ultraestrutura , Análise de Sequência de DNA/métodos , Clima TropicalRESUMO
Pseudotulostoma volvata (O. K. Mill. and T. W. Henkel) is a morphologically unusual member of the otherwise hypogeous Elaphomycetaceae due to its epigeous habit and exposed gleba borne on an elevated stalk at maturity. Field observations in Guyana indicated that P. volvata was restricted to rain forests dominated by ectomycorrhizal (EM) Dicymbe corymbosa (Caesalpiniaceae), suggesting an EM nutritional mode for the fungus. In this paper, we confirm the EM status of P. volvata with a combination of morphological, molecular, and mycosociological data. The EM status for P. volvata corroborates its placement in the ectotrophic Elaphomycetaceae.