RESUMO
In this study, the effects of different salinity gradients and addition of compatible solutes on anaerobic treated effluent water qualities, sludge characteristics and microbial communities were investigated. The increase in salinity resulted in a decrease in particle size of the granular sludge, which was concentrated in the range of 0.5-1.0 mm. The content of EPS (extracellular polymeric substances) in the granular sludge gradually increased with increasing salinity and the addition of betaine (a typical compatible solute). Meanwhile, the microbial community structure was significantly affected by salinity, with high salinity reducing the diversity of bacteria. At higher salinity, Patescibacteria and Proteobacteria gradually became the dominant phylum, with relative abundance increasing to 13.53% and 12.16% at 20 g/L salinity. Desulfobacterota and its subordinate Desulfovibrio, which secrete EPS in large quantities, dominated significantly after betaine addition.Their relative abundance reached 13.65% and 7.86% at phylum level and genus level. The effect of these changes on the treated effluent was shown as the average chemical oxygen demand (COD) removal rate decreased from 82.10% to 79.71%, 78.01%, 68.51% and 64.55% when the salinity gradually increased from 2 g/L to 6, 10, 16 and 20 g/L. At the salinity of 20 g/L, average COD removal increased to 71.65% by the addition of 2 mmol/L betaine. The gradient elevated salinity and the exogenous addition of betaine played an important role in achieving stability of the anaerobic system in a highly saline environment, which provided a feasible strategy for anaerobic treatment of organic saline wastewater.
Assuntos
Betaína , Salinidade , Esgotos , Eliminação de Resíduos Líquidos , Águas Residuárias , Betaína/metabolismo , Esgotos/microbiologia , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/química , Anaerobiose , Microbiota/efeitos dos fármacos , Bactérias/metabolismo , Bactérias/efeitos dos fármacosRESUMO
Salinity was considered to have effects on the characteristics, performance microbial communities of aerobic granular sludge. This study investigated granulation process with gradual increase of salt under different gradients. Two identical sequencing batch reactors were operated, while the influent of Ra and Rb was subjected to stepwise increments of NaCl concentrations (0-4 g/L and 0-10 g/L). The presence of filamentous bacteria may contribute to granules formed under lower salinity conditions, potentially leading to granules fragmentation. Excellent removal efficiency achieved in both reactors although there was a small accumulation of nitrite in Rb at later stages. The removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) in Ra were 95.31%, 93.70% and 88.66%, while the corresponding removal efficiencies in Rb were 94.19%, 89.79% and 80.74%. Salinity stimulated extracellular polymeric substances (EPS) secretion and enriched EPS producing bacteria to help maintain the integrity and stability of the aerobic granules. Heterotrophic nitrifying bacteria were responsible for NH4+-N and NO2--N oxidation of salinity systems and large number of denitrifying bacteria were detected, which ensure the high removal efficiency of TN in the systems.
Assuntos
Reatores Biológicos , Nitrogênio , Esgotos , Eliminação de Resíduos Líquidos , Eliminação de Resíduos Líquidos/métodos , Reatores Biológicos/microbiologia , Esgotos/microbiologia , Fósforo/metabolismo , Salinidade , Cloreto de Sódio , Bactérias/metabolismo , Microbiota , Análise da Demanda Biológica de OxigênioRESUMO
The multi-soil-layering (MSL) systems is an emerging solution for environmentally-friendly and cost-effective treatment of decentralized rural domestic wastewater. However, the role of the seemingly simple permeable layer has been overlooked, potentially holding the breakthroughs or directions to addressing suboptimal nitrogen removal performance in MSL systems. In this paper, the mechanism among diverse substrates (zeolite, green zeolite and biological ceramsite) coupled microorganisms in different systems (activated bacterial powder and activated sludge) for rural domestic wastewater purification was investigated. The removal efficiencies performed by zeolite coupled with microorganisms within 3 days were 93.8% for COD, 97.1% for TP, and 98.8% for NH4+-N. Notably, activated sludge showed better nitrification and comprehensive performance than specialized nitrifying bacteria powder. Zeolite attained an impressive 89.4% NH4+-N desorption efficiency, with a substantive fraction of NH4+-N manifesting as exchanged ammonium. High-throughput 16S rRNA gene sequencing revealed that aerobic and parthenogenetic anaerobic bacteria dominated the reactor, with anaerobic bacteria conspicuously absent. And the heterotrophic nitrification-aerobic denitrification (HN-AD) process was significant, with the presence of denitrifying phosphorus-accumulating organisms (DPAOs) for simultaneous nitrogen and phosphorus removal. This study not only raises awareness about the importance of the permeable layer and enhances comprehension of the HN-AD mechanism in MSL systems, but also provides valuable insights for optimizing MSL system construction, operation, and rural domestic wastewater treatment.
Assuntos
Eliminação de Resíduos Líquidos , Eliminação de Resíduos Líquidos/métodos , Nitrificação , Nitrogênio/metabolismo , Solo/química , Desnitrificação , Águas Residuárias/química , Esgotos/microbiologia , Microbiologia do Solo , Zeolitas/química , Fósforo/metabolismo , Reatores Biológicos/microbiologia , Bactérias/metabolismoRESUMO
Sewage sludge in cities of Yangzi River Belt, China, generally exhibits a lower organic content and higher silt contentdue to leakage of drainage system, which caused low bioenergy recovery and carbon emission benefits in conventional anaerobic digestion (CAD). Therefore, this paper is on a pilot scale, a bio-thermophilic pretreatment anaerobic digestion (BTPAD) for low organic sludge (volatile solids (VS) of 4%) was operated with a long-term continuous flow of 200 days. The VS degradation rate and CH4 yield of BTPAD increased by 19.93% and 53.33%, respectively, compared to those of CAD. The analysis of organic compositions in sludge revealed that BTPAD mainly improved the hydrolysis of proteins in sludge. Further analysis of microbial community proportions by high-throughput sequencing revealed that the short-term bio-thermophilic pretreatment was enriched in Clostridiales, Coprothermobacter and Gelria, was capable of hydrolyzing acidified proteins, and provided more volatile fatty acid (VFA) for the subsequent reaction. Biome combined with fluorescence quantitative polymerase chain reaction (PCR) analysis showed that the number of bacteria with high methanogenic capacity in BTPAD was much higher than that in CAD during the medium temperature digestion stage, indicating that short-term bio-thermophilic pretreatment could provide better methanogenic conditions for BTPAD. Furthermore, the greenhouse gas emission footprint analysis showed that short-term bio-thermophilic pretreatment could reduce the carbon emission of sludge anaerobic digestion system by 19.18%.
Assuntos
Esgotos , Eliminação de Resíduos Líquidos , Esgotos/microbiologia , Anaerobiose , Eliminação de Resíduos Líquidos/métodos , Projetos Piloto , Reatores Biológicos/microbiologia , Metano/metabolismo , Metano/análise , Carbono/metabolismo , Carbono/análise , China , BiocombustíveisRESUMO
The organic compound composition of wastewater, serves as a crucial indicator for the operational performance of activated sludge processes and has a major influence on the development of filamentous bulking in activated sludge. This study focused on the impact of typical soluble and slowly-biodegradable organic compounds, investigating the pathways through which these substrates affect the occurrence of filamentous bulking in systems operated under both high- and low-oxygen conditions. Results showed that slowly-biodegradable organic compounds lead to a concentrated distribution of microorganisms within flocs, with inward growth of filamentous bacteria. Both Tween-80 and granular starch treated systems exhibited a significant increase in protein content. The glucose system, utilizing soluble substrates, exhibited a markedly higher total polysaccharide content. Microbial communities in the Tween-80 and granular starch treated systems were characterized by a higher abundance of bacteria known to enhance sludge flocculation and settling, such as Competibacter, Xanthomonadaceae and Zoogloea. These findings are of high significance for controlling the operational performance and stability of activated sludge systems, deepening our understanding and providing a novel perspective for the improvement of wastewater treatment processes.
Assuntos
Biodegradação Ambiental , Esgotos , Eliminação de Resíduos Líquidos , Esgotos/microbiologia , Eliminação de Resíduos Líquidos/métodos , Floculação , Compostos Orgânicos/metabolismo , Águas Residuárias/química , Águas Residuárias/microbiologia , Bactérias/metabolismo , Reatores Biológicos/microbiologiaRESUMO
The increase of antibiotic-resistant bacteria has become a global health emergency and the need to explore alternative therapeutic options arises. Phage therapy uses bacteriophages to target specific bacterial strains. Phages are highly specific and can target resistant bacteria. Currently, research in this regard is focused on ensuring reliability and safety to bring this tool into clinical practice. The first step is to conduct comprehensive preclinical research. In this work, we present two novel bacteriophages vB_Kpn_F13 and vB_Kpn_F14 isolated against clinical carbapenem-resistant Klebsiella pneumoniae strains obtained from hospital sewage. Multiple studies in vitro were conducted, such as sequencing, electron microscopy, stability, host range infectivity, planktonic effect and biofilm inhibition in order to discover their ability to be used against carbapenem-resistant K. pneumoniae pathogens causing difficult-to-treat infections.
Assuntos
Bacteriófagos , Biofilmes , Enterobacteriáceas Resistentes a Carbapenêmicos , Carbapenêmicos , Infecções por Klebsiella , Klebsiella pneumoniae , Terapia por Fagos , Klebsiella pneumoniae/virologia , Klebsiella pneumoniae/efeitos dos fármacos , Bacteriófagos/isolamento & purificação , Bacteriófagos/fisiologia , Bacteriófagos/genética , Enterobacteriáceas Resistentes a Carbapenêmicos/isolamento & purificação , Enterobacteriáceas Resistentes a Carbapenêmicos/virologia , Infecções por Klebsiella/microbiologia , Infecções por Klebsiella/terapia , Carbapenêmicos/farmacologia , Biofilmes/crescimento & desenvolvimento , Biofilmes/efeitos dos fármacos , Humanos , Especificidade de Hospedeiro , Esgotos/virologia , Esgotos/microbiologia , Antibacterianos/farmacologia , Genoma Viral , Testes de Sensibilidade MicrobianaRESUMO
Global sewage sludge production is rapidly increasing, and its safe disposal is becoming an increasingly serious issue. One of the main methods of municipal sewage sludge management is based on its agricultural use. The wastewater and sewage sludge contain numerous antibiotic resistance genes (ARGs), and its microbiome differs significantly from the soil microbial community. The aim of the study was to assess the changes occurring in the soil microbial community and resistome after the addition of sewage sludge from municipal wastewater treatment plant (WWTP) in central Poland, from which the sludge is used for fertilizing agricultural soils on a regular basis. This study used a high-throughput shotgun metagenomics approach to compare the microbial communities and ARGs present in two soils fertilized with sewage sludge. The two soils represented different land uses and different physicochemical and granulometric properties. Both soils were characterized by a similar taxonomic composition of the bacterial community, despite dissimilarities between soils properties. Five phyla predominated, viz. Planctomycetes, Actinobacteria, Proteobacteria, Chloroflexi and Firmicutes, and they were present in comparable proportions in both soils. Network analysis revealed that the application of sewage sludge resulted in substantial qualitative and quantitative changes in bacterial taxonomic profile, with most abundant phyla being considerably depleted and replaced by Proteobacteria and Spirochaetes. In addition, the ratio of oligotrophic to copiotrophic bacteria substantially decreased in both amended soils. Furthermore, fertilized soils demonstrated greater diversity and richness of ARGs compared to control soils. The increased abundance concerned mainly genes of resistance to antibiotics most commonly used in human and animal medicine. The level of heavy metals in sewage sludge was low and did not exceed the standards permitted in Poland for sludge used in agriculture, and their level in fertilized soils was still inconsiderable.
Assuntos
Agricultura , Fertilizantes , Microbiota , Esgotos , Microbiologia do Solo , Solo , Esgotos/microbiologia , Agricultura/métodos , Solo/química , Microbiota/genética , Microbiota/efeitos dos fármacos , Bactérias/genética , Bactérias/efeitos dos fármacos , Bactérias/classificação , Metagenômica/métodos , Resistência Microbiana a Medicamentos/genética , PolôniaRESUMO
Sewage surveillance is a cost-effective tool for assessing antimicrobial resistance (AMR) in urban populations. However, research on sewage AMR in remote areas is still limited. Here, we used shotgun metagenomic sequencing to profile antibiotic resistance genes (ARGs) and ARG-carrying pathogens (APs) across 15 cities in Tibetan Plateau (TP) and the major cities in eastern China. Notable regional disparities in sewage ARG composition were found, with a significantly higher ARG abundance in TP (2.97 copies/cell). A total of 542 and 545 APs were identified in sewage from TP and the East, respectively, while more than 40 % carried mobile genetic elements (MGEs). Moreover, 65 MGEs-carrying APs were identified as World Health Organization (WHO) priority-like bacterial and fungal pathogens. Notably, a fungal zoonotic pathogen, Enterocytozoon bieneusi, was found for the first time to carry a nitroimidazole resistance gene (nimJ). Although distinct in AP compositions, the relative abundances of APs were comparable in these two regions. Furthermore, sewage in TP was found to be comparable to the cities in eastern China in terms of ARG mobility and AMR risks. These findings provide insights into ARGs and APs distribution in Chinese sewage and stress the importance of AMR surveillance and management strategies in remote regions.
Assuntos
Cidades , Metagenômica , Esgotos , Esgotos/microbiologia , Tibet , China , Resistência Microbiana a Medicamentos/genética , Bactérias/genética , Bactérias/efeitos dos fármacos , Farmacorresistência Bacteriana/genética , Antibacterianos/farmacologia , Genes BacterianosRESUMO
Antimicrobial resistance (AMR) is recognized as a global threat. AMR bacteria accumulate in sewage sludge however, knowledge on the persistence of human pathogens and AMR in the sludge line of the wastewater treatment is limited. Sludge can be used, with or without additional treatment, as fertilizer in agricultural fields. The aim of this study is to obtain knowledge about presence of human pathogens and AMR in the sewage sludge, before and after the anaerobic digestion (AD) applying innovative combinations of methods. Fifty sludge samples were collected. Cultivation methods combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and Antibiotic Susceptibility Test (AST) were used obtaining knowledge about the microbial community, pathogens, and antibiotic resistant bacteria while the droplet digital Polymerase Chain Reaction (ddPCR) was performed to detect most common AMR genes. In total, 231 different bacterial species were identified in the samples. The most abundant species were spore-forming facultative anaerobic bacteria belonging to Bacillus and Clostridium genera. The AD causes a shift in the microbial composition of the sludge (p = 0.04). Seven pathogenic bacterial species constituting 188 colonies were isolated and tested for susceptibility to Clindamycin, Meropenem, Norfloxacin, Penicillin G, and Tigecycline. Of the Clostridium perfringens and Bacillus cereus isolates 67 and 50 %, respectively, were resistant to Clindamycin. Two B. cereus and two C. perfringens isolates were also resistant to other antibiotics showing multidrug resistance. ARGs (blaOXA, blaTEM, ermB, qnrB, tet(A)-(W), sulI-II) were present at 7-8 Log gene copies/kg of sludge. AD is the main driver of a reduction of some ARGs (1 Log) but resistant bacteria were still present. The results showed the usefulness of the integration of the proposed analytical methods and suggest a decrease in the risk of presence of cultivable pathogens including resistant isolates after AD but a persistent risk of ARGs' horizontal transmission.
Assuntos
Antibacterianos , Esgotos , Esgotos/microbiologia , Anaerobiose , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/genética , Bactérias/isolamento & purificação , Testes de Sensibilidade Microbiana , Farmacorresistência Bacteriana , Águas Residuárias/microbiologiaRESUMO
Anammox bacteria (AnAOB) can be easily enriched under high temperatures and high substrate concentrations, while the application of the mainstream anammox process in low substrate municipal sewage is still relatively uncommon. Therefore, this study investigated the enrichment of AnAOB under conditions of low ammonia nitrogen and nitrite concentration at 25 °C. Results showed that using inoculated aerobic sludge, four ASBRs (R1, R2, R3 and R4) were successfully initiated with different influent substrate (NO2--N/NH4+-N) ratios of 1.2, 1.32, 1.4 and 1.5, respectively, with reactor start-up times were 162, 150, 120 and 134 days, respectively. The values of ΔNO2--N/ΔNH4+-N in reactors were stable at 1.17, 1.32, 1.43 and 1.53 respectively. The increase in influent substrate ratios resulted in improved TN removal rates and accelerated consumption of chemical oxygen demand (COD) during the initial start-up stage. The maximum TN removal rates achieved in the four reactors were 76.09%, 79.24%, 82.82% and 82.63%, respectively. The color of sludge gradually changes from yellowish-brown to reddish-brown. Furthermore, the surface of sludge exhibited a porous mineral structure, with crater-like cavities. The dominant anammox species in the system was identified as Candida Brocadia (3.04%). According to qPCR, the abundance of hzsB in the system is 1.65 × 1012 copies/g VSS, confirming the effective enrichment of AnAOB.
Assuntos
Amônia , Bactérias , Reatores Biológicos , Esgotos , Eliminação de Resíduos Líquidos , Reatores Biológicos/microbiologia , Esgotos/microbiologia , Bactérias/metabolismo , Eliminação de Resíduos Líquidos/métodos , Amônia/metabolismo , Nitritos/metabolismo , Nitrogênio/metabolismo , Análise da Demanda Biológica de Oxigênio , Compostos de Amônio/metabolismo , Oxirredução , AnaerobioseRESUMO
The impact of microplastics (MPs) in anaerobic wastewater treatment on microbial metabolism is significant. Anaerobic granular sludge (AS) and biofilm (BF) are two common ways, and their responses to microplastics will have a direct impact on their application potential. This study investigated the microbial reactions of AS and BF to three types of MPs: polyethylene (PE), polyvinyl chloride (PVC), and a mixture of both (MIX). Results exhibited that MPs reduced methane output by 44.65 %, 55.89 %, and 53.18 %, elevated short-chain fatty acid (SCFA) levels by 95.93 %, 124.49 %, and 110.78 %, and lowered chemical oxygen demand (COD) removal by 28.77 %, 36.78 %, and 33.99 % for PE-MP, PVC-MP, and MIX-MP, respectively, with PVC-MP showing the greatest inhibition. Meanwhile, microplastics also facilitated the relative production of reactive oxygen species (ROS, 40.29 %-96.99 %), lactate dehydrogenase (LDH, 20.01 %-75.02 %), and adenosine triphosphate (ATP, 26.64 %-43.80 %), while reducing cytochrome c (cyt c, 23.60 %-49.02 %) and extracellular polymeric substances (EPS, 17.44 %-26.58 %). AS and BF displayed distinct enzymatic activities under MPs exposure. Correspondingly, 16S-rRNA sequencing indicated that AS was mainly involved in acetate generation by Firmicutes, while BF performed polysaccharide degradation by Bacteroidota. Metatranscriptomic analysis showed AS to be rich in acetogens (Bacillus, Syntrophobacter) and methanogens (Methanothrix, Methanobacterium), while BF contained more fermentation bacteria (Mesotoga, Lentimicrobium) and electroactive microorganisms (Clostridium, Desulfuromonas) under MIX-MP. Moreover, BF exhibited higher glycolysis gene expression, whereas AS was more active in methane metabolism, primarily through the acetoclastic methanogenic pathway's direct acetate conversion. This study provides new insights into understanding the microbial response produced by microplastics during anaerobic wastewater digestion.
Assuntos
Microplásticos , Eliminação de Resíduos Líquidos , Águas Residuárias , Poluentes Químicos da Água , Águas Residuárias/microbiologia , Microplásticos/toxicidade , Anaerobiose , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/metabolismo , Fermentação , Biofilmes , Esgotos/microbiologiaRESUMO
BACKGROUND: Bacteriophage has been renewed attention as a new antibacterial agent due to the limitations of antibiotic treatment. Bacteriophages are generally thought to be highly host specific and even strain specific, but a small number of polyvalent bacteriophages have been found to infect bacteria of different genera. RESULTS: In this study, a virulent lytic bacteriophage (named Salmonella phage PSH-1) of Salmonella Enteritidis was isolated from the sewage samples of a large-scale pig farm, PSH-1 demonstrated lytic activity against four multidrug-resistant Salmonella Enteritidis isolates and Escherichia coli, and then its biological characteristics, genome and bacteriostatic ability were investigated. The results showed that the initial titer of PSH-1 was 1.15 × 1010 PFU/mL and the optimal multiplicity of infection (MOI) was 0.01, PSH-1 has stable activity in the range of pH 3.0-11.0. One-step growth curve showed that its latent period was 20 min, burst time was 80 min, and the burst was 495 particles. The whole-genome sequencing results revealed phage PSH-1 had a linear dsDNA with 48,466 bp length. The G/C content was 45.33%. Non-coding RNA genes and virulence factors were not found. Eighty- five open reading frames (ORFs) were identified after online annotation. By tests, the use of phage could succeed in controlling the artificial Salmonella contamination in milk at a range of temperatures. CONCLUSIONS: This study reports a novel Salmonella Enteritidis phage PSH-1, which has a robust lytic ability, no virulence factors, and good stability. The characterization and genomic analysis of PSH-1 will develop our understanding of phage biology and diversity and provide a potential arsenal for controlling of salmonellosis.
Assuntos
Farmacorresistência Bacteriana Múltipla , Genoma Viral , Fagos de Salmonella , Salmonella enteritidis , Esgotos , Sequenciamento Completo do Genoma , Salmonella enteritidis/virologia , Salmonella enteritidis/genética , Salmonella enteritidis/efeitos dos fármacos , Fagos de Salmonella/genética , Fagos de Salmonella/isolamento & purificação , Fagos de Salmonella/fisiologia , Fagos de Salmonella/classificação , Farmacorresistência Bacteriana Múltipla/genética , Animais , Esgotos/virologia , Esgotos/microbiologia , Suínos , Composição de Bases , Escherichia coli/virologia , Escherichia coli/genéticaRESUMO
Objective: To express and purify the phage depolymerase from hypervirulent Klebsiella pneumoniae (hvKp) serotype K1 and validate its function. Methods: Phage that infected serotype K1-type hvKp was isolated from hospital sewage. The biology and morphology of the phage were determined by plaque assay and transmission electron microscopy. The whole genome of the phage was sequenced by the Illumina HiSeq 2500 platform. The presence of depolymerase was determined by observing the plaque halo. Bioinformatic analysis and prokaryotic protein expression system were further used to predict and identify phage depolymerase. The depolymerase gene fragment was obtained by PCR and cloned into the pET28a expression vector, and the expression and purification of the depolymerase were completed in strain BL21. The depolymerase activities on the capsular polysaccharide of serotype K1-type hvKp clinical isolates were detected by plaque assay and low-speed centrifugation assay. Results: A lytic phage (phiA2) that infected serotype K1-type hvKp clinical isolate was isolated from hospital sewage. It was typical of the Caudovirales order and Autographiviridae family, and its whole genome was 43 526 bp in length and contained 51 coding domain sequences. The phage phiA2-derived depolymerase phiA2-dep was predicted, expressed and purified. The plaque assay and low-speed centrifugation assay indicated that the depolymerase phiA2-dep had good lytic activity on the capsular polysaccharide of serotype K1-type hvKp clinical isolates. Conclusion: Depolymerase phiA2-dep can specifically degrade the capsular polysaccharide of serotype K1-type hvKp, which has potential application value in treating bacterial infection.
Assuntos
Bacteriófagos , Klebsiella pneumoniae , Sorogrupo , Klebsiella pneumoniae/genética , Bacteriófagos/genética , Bacteriófagos/isolamento & purificação , Esgotos/microbiologia , Genoma ViralRESUMO
Anaerobic electrochemical digestion (AED) is an effective system for recovering biogas from organic wastes. However, the effects of different anode potentials on anaerobic activated sludge remain unclear. This study confirmed that biofilms exhibited the best electroactivity at -0.2 V (vs. Ag/AgCl) compared to -0.4 V and 0 V. Gas was further regulated, with the highest hydrogen content (47 ± 7 %) observed at -0.2 V. The 0 V system produced the largest amount of methane (70 ± 8 %) and exhibited the greatest presence of hydrogen-utilizing microorganisms. The gas yield at -0.4 V was the lowest, with no hydrogen detected. Excess bioelectrohydrogen at -0.2 V and 0 V caused the co-enrichment of Methanobacterium and Acetoanaerobium, establishing a thermodynamically feasible current-acetate-hydrogen electron cycle to improve electrogenesis. These results provide insights into the regulatory strategies of MEC technology during anaerobic digestion, which play a decisive role in determining the composition of biogas.
Assuntos
Eletrodos , Hidrogênio , Metano , Microbiota , Anaerobiose , Hidrogênio/metabolismo , Metano/metabolismo , Microbiota/fisiologia , Gases , Esgotos/microbiologia , Biocombustíveis , Técnicas Eletroquímicas/métodos , BiofilmesRESUMO
Sewer pipe materials exhibit diverse inner-surface features, which can affect the attachment of biofilm and influence microbial metabolic processes. To investigate the role of the type of pipe material on the composition and metabolic capabilities of the adhering microorganisms, three sets of urban sewers (High-Density Polyethylene Pipe (HDPE), Ductile Iron Pipe (DIP), and Concrete Pipe (CP)) were constructed. Measurements of biofilm thickness and environmental factors revealed that the thickest biofilm in CP pipes reached 2000 µm, with ORP values as low as -325 mV, indicating a more suitable anaerobic microbial habitat. High-throughput sequencing showed similar relative abundances of genera related to carbon and sulfur metabolism in the DIP and CP pipes, whereas HDPE exhibited only half the relative abundance compared to that found in the other pipes. To explore the impact of pipe materials on the mechanisms of microbial response, a metagenomic approach was used to investigate the biological transformation of carbon and sulfur in wastewater. The annotations of the crucial enzyme-encoding genes related to methyl coenzyme M and sulfite reductase in DIP and CP were 50 and 110, respectively, whereas HDPE exhibited lower counts (25 and 70, respectively). This resulted in significantly lower carbon and sulfur metabolism capabilities in the HDPE biofilm than in the other two pipes. The stability of wastewater quality during the transmission process in HDPE pipes reduces the metabolic generation of toxic and harmful gases within the pipes, favoring the preservation of carbon sources for sewer systems. This study reveals the variations in carbon and sulfur metabolism in wastewater pipe systems influenced by pipe materials and provides insights for designing future sewers.
Assuntos
Biofilmes , Esgotos/microbiologia , Eliminação de Resíduos Líquidos/métodos , Águas ResiduáriasRESUMO
Growing human population and increasing urbanization call for the need for proper wastewater treatment to reduce environmental pollution and reduce the excess use of natural resources. During the collection of municipal wastewater, the rapid aerobic respiration often causes oxygen depletion and anaerobic conditions in the sewer system resulting in the production of malodorous compounds. The odor problems may lead to public complaints, or in the case of the sewage workers the released volatile compounds even cause serious health hazards. Therefore, microbes have a dual contribution in the urban water cycle, since they have a decisive role in wastewater treatment and the removal of pollutants, but they can also cause problems in the artificial environment. In this review, I would like to summarize the processes underlying the generation of the bad smell associated with sewage and wastewater or with the collection and treatment infrastructure, tracking the way from the households to the plants, including the discussion of processes and possible mitigation related to the released hydrogen sulfide, volatile organics and other compounds.
Assuntos
Odorantes , Esgotos , Esgotos/microbiologia , Odorantes/análise , Humanos , CidadesRESUMO
Salmonella is a globally prevalent foodborne pathogen, and adverse events caused by S. Enteritidis and S. Typhimurium are extremely common. With the emergence of drug resistance, there is an urgent need for efficient and specific lytic bacteriophages as alternative to antibiotics in clinical practice. In this study, phage P6 was isolated and screened from effluent and fecal samples from duck farm environments to specifically lyse the duck sources S. Typhimurium and S. Enteritidis. Phage P6 belongs to the genus Lederbergvirus, unclassified Lederbergvirus species. The phage P6 genome did not contained non-coding RNA, virulence genes and drug resistance genes, indicating that phage P6 was biologically safe for clinical applications. Phage P6 lysed 77.78% (28/36) of multidrug-resistant Salmonella and reduced biofilms formed by S. Enteritidis CVCC 3377, 4, and 24, and S. Typhimurium 44 by 44% to 75% within 3 h, and decreased Salmonella in duckling feces by up to 1.64 orders of magnitude. Prokaryotic expression of endolysin LysP6 lysed the chloroform-treated bacterial outer membrane from different serotypes of duck-derived Salmonella and E. coli standard strain ATCC 25922. The host range was expanded compared to phage P6, and the growth of Salmonella was effectively inhibited by LysP6 in conjunction with the membrane permeabilizer EDTA within 24 h. Therefore, phage P6 and phage-derived endolysins LysP6 are suitable for application as potent biocontrol agents to improve poultry health and food safety.
Assuntos
Patos , Endopeptidases , Fagos de Salmonella , Salmonella typhimurium , Esgotos , Animais , Fagos de Salmonella/fisiologia , Esgotos/virologia , Esgotos/microbiologia , Endopeptidases/farmacologia , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/virologia , Salmonella enteritidis/efeitos dos fármacos , Salmonella enteritidis/virologia , Doenças das Aves Domésticas/microbiologia , Doenças das Aves Domésticas/virologia , Doenças das Aves Domésticas/prevenção & controle , Salmonelose Animal/microbiologia , Salmonelose Animal/prevenção & controle , Fezes/microbiologia , Fezes/virologiaRESUMO
Antimicrobial resistance (AMR) is a significant threat that demands surveillance to identify and analyze trends of the emerging antibiotic resistance genes (ARGs) and potential microbial carriers. The influent of the wastewater treatment plants (WWTPs) reflects the microbes derived from the population and effluent being the source of dissemination of potential pathogenic microbes and AMR. The present study aimed to monitor microbial communities and antibiotic resistance genes in WWTPs employing a whole metagenome shotgun sequencing approach. The samples were collected from a sewage treatment plant (STP) and a common effluent treatment plant (CETP) in Delhi, India. The results showed the influent of STP to be rich in Bifidobacterium, Bacteroides, Escherichia, Arcobacter, and Pseudomonas residents of gut microbiota and known to cause diseases in humans and animals; whereas the CETP sample was abundant in Aeromonas, Escherichia, and Shewanella known to be involved in the degradation of different compounds. Interestingly, the effluent samples from both STPs and CETP were rich in microbial diversity, comprising organic and xenobiotic compound degrading and disease-causing bacteria, indicating the effluent being the source of dissemination of concerning bacteria to the environment. The functional profile at both sites displayed similarity with an abundance of housekeeping function genes as analyzed by Clusters of Orthologous Genes (COG), KEGG Orthology (KO), and subsystem databases. Resistome profiling by MEGARes showed the dominance of ARGs corresponding to beta-lactams having relative abundance ranging from 16% to 34% in all the metagenome datasets, followed by tetracycline (8%-16%), aminoglycosides (7%-9%), multi-drug (5%-9%), and rifampin (3%-9%). Also, AMR genes oxa, ant3-DPRIME, and rpoB, which are of clinical importance were predominantly and most prevalently present in all the samples. The presence of AMR in effluents from both types of treatment plants indicates that wastewater from both sources contributes to the spread of pathogenic bacteria and resistance genes, increasing the environmental AMR burden and therefore requires tertiary treatment before discharge. This work will facilitate further research towards the identification of suitable biomarkers for monitoring antibiotic resistance.
Assuntos
Microbiota , Esgotos , Águas Residuárias , Índia , Esgotos/microbiologia , Águas Residuárias/microbiologia , Resistência Microbiana a Medicamentos/genética , Bactérias/genética , Bactérias/efeitos dos fármacos , Eliminação de Resíduos LíquidosRESUMO
Perfluorooctanoic acid (PFOA) as emerging pollutants was largely produced and stable in nature environment. Its fate and effect to the wasted sludge digestion process and corresponding microbial mechanism was rarely reported. This study investigated the different dose of PFOA to the wasted sludge digestion process, where the methane yield and microbial mechanism was illustrated. The PFOA added before digestion were 0-10000 µg/L, no significant variation in daily and accumulated methane production between each group. The 9th day methane yield was significantly higher than other days (p < 0.05). The soluble protein was significantly decreased after 76 days digestion (p < 0.001). The total PFOA in sludge (R2 = 0.8817) and liquid (R2 = 0.9083) phase after digestion was exponentially correlated with PFOA dosed. The PFOA in liquid phase was occupied 54.10 ± 18.38% of the total PFOA in all reactors. The dewatering rate was keep decreasing with the increase of PFOA added (R2 = 0.7748, p < 0.001). The mcrA abundance was significantly correlated with the pH value and organic matter concentration in the reactors. Chloroflexi was the predominant phyla, Aminicenantales, Bellilinea and Candidatus_Cloacimonas were predominant genera in all reactors. Candidatus_Methanofastidiosum and Methanolinea were predominant archaea in all reactors. The function prediction by FAPROTAX and Tax4fun implied that various PFOA dosage resulted in significant function variation. The fermentation and anaerobic chemoheterotrophy function were improved with the PFOA dose. Co-occurrence network implied the potent cooperation among the organic matter degradation and methanogenic microbe in the digestion system. PFOA has little impact to the methane generation while affect the microbe function significantly, its remaining in the digested sludge should be concerned to reduce its potential environmental risks.
Assuntos
Caprilatos , Fluorocarbonos , Metano , Esgotos , Metano/metabolismo , Fluorocarbonos/metabolismo , Anaerobiose , Esgotos/microbiologia , Caprilatos/metabolismo , Reatores BiológicosRESUMO
Candidatus Patescibacteria, also known as candidate phyla radiation (CPR), including the class-level uncultured clade JAEDAM01 (formerly a subclass of Gracilibacteria/GN02/BD1-5), are ubiquitous in activated sludge. However, their characteristics and relationships with other organisms are largely unknown. They are believed to be episymbiotic, endosymbiotic or predatory. Despite our understanding of their limited metabolic capacity, their precise roles remain elusive due to the difficulty in cultivating and identifying them. In previous research, we successfully recovered high-quality metagenome-assembled genomes (MAGs), including a member of JAEDAM01 from activated sludge flocs. In this study, we designed new probes to visualize the targeted JAEDAM01-associated MAG HHAS10 and identified its host using fluorescence in situ hybridization (FISH). The FISH observations revealed that JAEDAM01 HHAS10-like cells were located within dense clusters of Zoogloea, and the fluorescence brightness of zoogloeal cells decreased in the vicinity of the CPR cells. The Zoogloea MAGs possessed genes related to extracellular polymeric substance biosynthesis, floc formation and nutrient removal, including a polyhydroxyalkanoate (PHA) accumulation pathway. The JAEDAM01 MAG HHAS10 possessed genes associated with type IV pili, competence protein EC and PHA degradation, suggesting a Zoogloea-dependent lifestyle in activated sludge flocs. These findings indicate a new symbiotic relationship between JAEDAM01 and Zoogloea.