RESUMO
Mare endometrosis is a major reproductive problem associated with low fertility and is characterized by persistent inflammation, TGFß-1 signaling, and consequently, extracellular matrix deposition, which compromises endometrial glands. Mesenchymal stem cell-based products (MSCs), such as extracellular vesicles (EVs), have gained attention due to the regulatory effects exerted by their miRNA cargo. Here, we evaluated the impact of preconditioning equine adipose mesenchymal stem cells with TGFß-1 for short or long periods on the anti-fibrotic properties of secreted extracellular vesicles. MSCs were isolated from six healthy horses and exposed to TGFß-1 for 4, 24, and 0 h. The expression of anti-fibrotic and pro-fibrotic miRNAs and mRNAs in treated cells and miRNAs in the cargo of secreted extracellular vesicles was measured. The resulting EVs were added for 48 h to endometrial stromal cells previously induced to a fibrotic status. The expression of anti-fibrotic and pro-fibrotic genes and miRNAs was evaluated in said cells using qPCR and next-generation sequencing. Preconditioning MSCs with TGFß-1 for 4 h enriched the anti-fibrotic miRNAs (mir29c, mir145, and mir200) in cells and EVs. Conversely, preconditioning the cells for 24 h leads to a pro-fibrotic phenotype overexpressing mir192 and mir433. This finding might have implications for developing an EV-based protocol to treat endometrial fibrosis in mares.
Assuntos
Endométrio , Vesículas Extracelulares , Fibrose , Células-Tronco Mesenquimais , MicroRNAs , Fator de Crescimento Transformador beta1 , Animais , Cavalos , Feminino , Células-Tronco Mesenquimais/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Vesículas Extracelulares/metabolismo , Fator de Crescimento Transformador beta1/metabolismo , Fator de Crescimento Transformador beta1/genética , Endométrio/metabolismo , Endométrio/citologia , Tecido Adiposo/citologia , Tecido Adiposo/metabolismo , Células Estromais/metabolismo , Células Estromais/efeitos dos fármacos , Doenças dos Cavalos , Regulação da Expressão Gênica/efeitos dos fármacos , Endometriose/veterinária , Endometriose/metabolismo , Endometriose/genéticaRESUMO
Purpose: To evaluate the effects of the nutraceuticals omega-6/3 and omega-9/6 on endometriosis-associated infertility and pain. Methods: Controlled experimental study, with each group composed of eight female rats. Fertility groups: sham-operated control (0.9% saline solution); control with endometriosis (0.9% saline); omega-6/3 (1.2 g/kg/day); omega-9/6 (1.2 g/kg/day); and meloxicam (0.8 mg/kg/day). Pain groups: sham-operated control (0.9% saline); control with endometriosis (0.9% saline); omega-6/3 (1.2 g/kg/day); omega-9/6 (1.2 g/kg/day); medroxyprogesterone acetate (5 mg/kg/every 3 days); and meloxicam (0.8 mg/kg/day). Peritoneal endometriosis was surgically induced. Pain was evaluated with the writhing test. Fertility was evaluated by counting the number of embryos in the left hemi-uterus. Results: The mean number of writhings was as follows: sham-operated, 11.1 ± 2.9; control with endometriosis, 49.3 ± 4.4; omega-6/3, 31.5 ± 2.7; omega-9/6, 34.1 ± 4.5; medroxyprogesterone acetate, 2.1 ± 0.8; meloxicam, 1 ± 0.3. There was a significant difference between both controls and all drugs used for treatment. Regarding fertility, the mean values were as follows: sham-operated, 6.8 ± 0.6; control with endometriosis, 4.2 ± 0.7; omega-6/3, 4.7 ± 1; omega-9/6, 3.8 ± 0.9; and meloxicam, 1.8 ± 0.9. Conclusions: The omega-6/3 and omega-9/6 nutraceuticals decreased pain compared to the controls. There was no improvement in fertility in any of the tested groups.(AU)
Assuntos
Animais , Feminino , Ratos , Endometriose/veterinária , Ácidos Graxos Ômega-3/uso terapêutico , Ácidos Graxos Ômega-6/uso terapêutico , Infertilidade Feminina/veterinária , Dor/tratamento farmacológico , Suplementos NutricionaisRESUMO
Reproductive diseases, mainly endometritis, are important hurdles in cattle raising, In the current study we evaluated gross, bacteriological, cytological, and histological findings from selected sites of the genital from 23 slaughtered cows and tested whether there is an association between these findings and the probability of reaching a reliable diagnosis. The results from the examinations of macroscopic aspects of uterine secretions, the cytological, bacteriological, and histopathological findings were then correlated. There was no significant correlation (P>0.05) of the statistical data from different parts of the genital tract. Trueperella pyogenes and Escherichia coli were isolated from the vagina in 3/23 cases. In only 2/23 samples Enterococcus faecalis and a gram-negative, oxidase-positive bacteria were isolated from the cervix uteri. Only Staphylococcus epidermidis, in 1/23 case, was isolated from the uterus. Histopathological findings in uterus from samples of Groups II (moderate lesions) and III (severe lesions) did not translated in grossly visible changes. Samples from reproductive tracts with secretion in the vagina and cervix uteri had no detectable changes in the other parameters analyzed from this portion. Uterus with positive bacterial culture had evidence of ascendant inflammation judging by the high granulocyte count in the three analyzed portions. This study reinforces that vaginitis and cervicitis in the cow diagnosed only by clinical examination does not reflect the real status of the uterine health. For this reason, treatment of uterine disorders should be conducted based on reliable tests to determine the appropriate therapy for each situation.(AU)
Doenças reprodutivas causam altas perdas econômicas nos rebanhos bovinos, sendo a endometrite uma das alterações mais relevantes. Os objetivos desta pesquisa foram avaliar a associação dos achados fisiopatológicos em exames macroscópicos, bacteriológicos, citológicos e histopatológicos nas porções do trato genital de 23 vacas abatidas, bem como avaliar a necessidade de associação destes exames para efetuar diagnóstico fidedigno. A avaliação macroscópica da secreção, os exames histopatológico, citológico e as bactérias identificadas foram correlacionados. Não houve associação (P>0,05) dos resultados nas diferentes porções do trato genital. Na vagina foram isoladas as bactérias Trueperella pyogenes e Escherichia coli. Na cérvix, em apenas 2/23 (8,6%) amostras isolou-se Enterococcus faecalis e gram negativo oxidase positiva. No útero houve isolamento apenas da bactéria Staphylococcus epidermidis. As amostras histopatológicas classificadas em grupo II e III não apresentaram alterações detectadas no exame macroscópico. As amostras com secreção não fisiológica na vagina e cérvix não apresentaram alterações nos outros exames na porção uterina. A amostra com cultura bacteriológica positiva no útero demonstrou uma infecção ascendente através da alta contagem de granulócitos nas três porções analisadas. O presente estudo reforça o conceito de que a vaginite e cervicite diagnosticadas pelo exame clinico na vaca não retratam o real status da saúde uterina e por esta razão, o tratamento do útero deve ser conduzido com critério, alicerçado nos exames complementares para definir a terapia adequada para cada situação.(AU)
Assuntos
Animais , Feminino , Bovinos , Endometriose/diagnóstico , Endometriose/veterinária , Genitália Feminina/microbiologia , Técnicas Citológicas/veterinária , Granulócitos , Infecções do Sistema Genital/veterinária , Cervicite Uterina/veterinária , Vaginose Bacteriana/veterináriaRESUMO
Reproductive diseases, mainly endometritis, are important hurdles in cattle raising, In the current study we evaluated gross, bacteriological, cytological, and histological findings from selected sites of the genital from 23 slaughtered cows and tested whether there is an association between these findings and the probability of reaching a reliable diagnosis. The results from the examinations of macroscopic aspects of uterine secretions, the cytological, bacteriological, and histopathological findings were then correlated. There was no significant correlation (P>0.05) of the statistical data from different parts of the genital tract. Trueperella pyogenes and Escherichia coli were isolated from the vagina in 3/23 cases. In only 2/23 samples Enterococcus faecalis and a gram-negative, oxidase-positive bacteria were isolated from the cervix uteri. Only Staphylococcus epidermidis, in 1/23 case, was isolated from the uterus. Histopathological findings in uterus from samples of Groups II (moderate lesions) and III (severe lesions) did not translated in grossly visible changes. Samples from reproductive tracts with secretion in the vagina and cervix uteri had no detectable changes in the other parameters analyzed from this portion. Uterus with positive bacterial culture had evidence of ascendant inflammation judging by the high granulocyte count in the three analyzed portions. This study reinforces that vaginitis and cervicitis in the cow diagnosed only by clinical examination does not reflect the real status of the uterine health. For this reason, treatment of uterine disorders should be conducted based on reliable tests to determine the appropriate therapy for each situation.(AU)
Doenças reprodutivas causam altas perdas econômicas nos rebanhos bovinos, sendo a endometrite uma das alterações mais relevantes. Os objetivos desta pesquisa foram avaliar a associação dos achados fisiopatológicos em exames macroscópicos, bacteriológicos, citológicos e histopatológicos nas porções do trato genital de 23 vacas abatidas, bem como avaliar a necessidade de associação destes exames para efetuar diagnóstico fidedigno. A avaliação macroscópica da secreção, os exames histopatológico, citológico e as bactérias identificadas foram correlacionados. Não houve associação (P>0,05) dos resultados nas diferentes porções do trato genital. Na vagina foram isoladas as bactérias Trueperella pyogenes e Escherichia coli. Na cérvix, em apenas 2/23 (8,6%) amostras isolou-se Enterococcus faecalis e gram negativo oxidase positiva. No útero houve isolamento apenas da bactéria Staphylococcus epidermidis. As amostras histopatológicas classificadas em grupo II e III não apresentaram alterações detectadas no exame macroscópico. As amostras com secreção não fisiológica na vagina e cérvix não apresentaram alterações nos outros exames na porção uterina. A amostra com cultura bacteriológica positiva no útero demonstrou uma infecção ascendente através da alta contagem de granulócitos nas três porções analisadas. O presente estudo reforça o conceito de que a vaginite e cervicite diagnosticadas pelo exame clinico na vaca não retratam o real status da saúde uterina e por esta razão, o tratamento do útero deve ser conduzido com critério, alicerçado nos exames complementares para definir a terapia adequada para cada situação.(AU)
Assuntos
Animais , Feminino , Bovinos , Endometriose/diagnóstico , Endometriose/veterinária , Genitália Feminina/microbiologia , Técnicas Citológicas/veterinária , Granulócitos , Infecções do Sistema Genital/veterinária , Cervicite Uterina/veterinária , Vaginose Bacteriana/veterináriaRESUMO
Persistent mating-induced endometritis (PMIE) results in decreased fertility in horses, thereby causing a significant impact in the horse market. Platelet-rich plasma (PRP), a modulator of the inflammatory response, has been largely used in veterinary medicine. Here, we investigated the effects of PRP on uterine inflammation, conception rate, endometrial polymorphonuclear neutrophil (PMN) migration, and COX-2 protein levels in the endometrial tissue. Thirteen PMIE-susceptible mares were used for artificial insemination (AI). The mares were inseminated with fresh semen in three consecutive cycles in a cross-over study design. The following cycle classifications were used: control cycle, no pharmacological interference; pre-AI, 20 mL of PRP was infused 24 h before AI; and post-AI, 20 mL of PRP was infused four h after AI. Follicular dynamics were monitored daily by transrectal ultrasound. When a follicle larger than 35 mm was detected, ovulation was induced with deslorelin acetate (1 mg, im). AI was performed 24 h after ovulation induction. Intrauterine fluid (FLU) was evaluated by ultrasonography before and 24 h after AI. PMNs in uterine cytology (CYT) and biopsy (HIS) were also observed before and 24 h after AI. Pregnancy was determined within 14 days after ovulation. Number of COX-2 positive cells was evaluated by immunohistochemistry. Both PRP treatments resulted in a decrease of PMNs in the CYT after breeding when compared to controls. FLU did not differ between cycles; however, the conception rates were significantly higher in the PRP mares. Mares positive for endometritis decreased in both treatment groups, and a more intense positive COX-2 labeling was observed in the control group when compared to the two treatment groups. In conclusion, PRP beneficially reduces inflammatory response in PMIE mares independent of when treatments were administered, thus increasing chances of successful pregnancy.
Assuntos
Endometriose/veterinária , Endométrio/metabolismo , Doenças dos Cavalos/prevenção & controle , Cavalos/fisiologia , Inseminação Artificial/veterinária , Leucócitos/efeitos dos fármacos , Animais , Movimento Celular/fisiologia , Endometriose/prevenção & controle , Endométrio/efeitos dos fármacos , Feminino , Leucócitos/fisiologia , Plasma Rico em Plaquetas , Gravidez , Taxa de GravidezRESUMO
PURPOSE:To investigate the efficacy of intralesional 20% aspirin injection for treatment of experimental peritoneal endometriosis.Methods:Peritoneal endometriosis was experimentally induced in forty adult nulligravid female rabbits. On day 30 post-endometriosis induction, rabbits were randomly divided to assess early (10 days) and late (20 days) effects of intralesional injection of physiological saline solution (control groups) in comparison to intralesional injection of 20% bicarbonate aspirin solution (experimental groups) as follows: control group 1 (10 days, n=10); control group 2 (20 days, n=10); experimental group 3 (10 days, n=10); experimental group 4 (20 days, n=10). Resected tissues, including endometriosis foci, were qualitatively (general morphology and signs of inflammatory cells infiltrate, necrosis and apoptosis) and quantitatively (remaining endometriosis area) assessed by histopathological analysis.Results:Extensive necrosis, hemorrhage, apoptosis, and fibrosis were observed in the experimental groups 3 and 4. Groups 1 and 2 presented typical endometrial tissue cysts, respectively. Groups 3 and 4 showed sparse endometrial tissue foci and no endometrial tissue, respectively. Quantitative analysis revealed that aspirin-treated groups 3 and 4 had significantly (p<0.05) smaller remaining endometriosis area, compared to control groups 1 and 2.Conclusion:Intralesional 20% aspirin injection caused total destruction of peritoneal endometriosis foci in rabbits.(AU)
Assuntos
Animais , Feminino , Coelhos , Endometriose/terapia , Endometriose/veterinária , Doenças Peritoneais/veterinária , Aspirina/uso terapêuticoRESUMO
BACKGROUND: Endometriosis is ectopic development of endometrial glands and stroma in extra-uterine sites and if the lesions occur as a well-defined mass is referred to as endometrioma. In the literature, endometrioma has been reported in only women and there are no reports of endometrioma in animals, including non-human primates. CASE PRESENTATION: A rare case of endometrioma is reported in an 11-year-old female German Shepherd with clinical signs of dehydration, anemia and prostration. Necropsy revealed ascites, generalized pallor, and a well-demarcated reddish mass adjacent to the left ovary and uterus and adherent to the retroperitoneum. The mass measured 25.0 × 20.0 cm with intermingled soft and firm areas. Upon incision, the mass was found to be solid with variable sized cystic cavities filled with coagulated blood. Microscopically, the mass was composed of cuboidal or prismatic epithelial cells arranged in tubules or acini. The epithelium of the mass had similar characteristics to the normal endometrium with PAS-positive secretions. The stroma was prominent and formed by loose connective tissue and smooth muscle fibers as confirmed by Masson trichrome. Extensive multifocal areas of hemorrhage were also observed in the stroma of the mass and in the interior of some epithelium-lined, cystic structures. Most of the epithelial cells had strong and diffuse cytokeratin expression, and some had vimentin expression. Epithelial and stromal cells also showed ERß, AR, VEGF and COX2 expression. The stroma showed areas with strong and diffuse vimentin expression. Factor VIII expression was observed only in the endothelium of blood vessels in the stroma. CONCLUSIONS: The macroscopic, microscopic and immunohistochemical findings are consistent with an endometrioma.
Assuntos
Doenças do Cão/diagnóstico , Endometriose/veterinária , Doenças Ovarianas/veterinária , Doenças Uterinas/veterinária , Animais , Doenças do Cão/patologia , Cães , Endometriose/diagnóstico , Endometriose/patologia , Feminino , Imuno-Histoquímica/veterinária , Doenças Ovarianas/diagnóstico , Doenças Ovarianas/patologia , Doenças Uterinas/diagnóstico , Doenças Uterinas/patologiaRESUMO
Dairy cows have shown a steep increase in milk production, which is unfortunately characterized by a dramatic decline in reproductive performance. In these modern high yielding cows authors worldwide mention a higher number of puerperal problems, such as retained placenta, acute metritis and abnormal vaginal discharge. All of which are known to negatively influence reproductive capacity of these animals. Cows affected by retained placenta and/or acute metritis are furthermore at a significantly higher risk of other typical dairy cow diseases such as acetonaemia, displaced abomasum and cystic ovarian disease. It is therefore important that the management of the cows during the transition period should be optimized in order to prevent cows from these disease complexes. Furthermore, during the immediate postpartum period, cows should be looked after properly in order to identify animals that are affected by clinical uterine disease as soon as possible so that treatment should not be delayed. High numbers of cows are furthermore reported to suffer from clinical and subclinical endometritis, both giving rise to significant problems to get in calf. At the moment, there is a lot of debate concerning both the diagnosis as well as the first choice treatment of cows suffering from clinical and subclinical endometritis. Therefore, also for these diseases prevention is far more effective than treatment.
Vacas de leite tem mostrado um aumento marcante na produção de leite, que infelizmente é caracterizado por um declínio dramático no desempenho reprodutor. Com relação a estas vacas de leite modernas de alto rendimento autores ao redor do mundo mencionam diversos problemas de puerpério, como placenta retida, metrite aguda e descarga vaginal anormal. Todos estes conhecidamente afetam a capacidade reprodutora destes animais. Vacas afetadas por placenta retida e/ou metrite aguda estão também em maior risco de outros tipos de doenças de vacas de leite como acetonaemia, abomaso deslocado e doença de ovário cístico. É importante, portanto, que o gerenciamento de vacas durante o período de transição deve ser otimizado para prevenir vacas contra estas doenças complexas. Além disto, durante o período pós parto imediato deve haver o cuidado apropriado das vacas para identificar animais afetados por doença uterina clínica assim que possível para não atrasar o tratamento. Um alto número de vacas sofrem de endometriose clínica e subclínica, ambos levando a problemas significativos para reproduzir. No momento há muito debate com relação ao diagnóstico e também ao primeiro tratamento de vacas sofrendo de endometriose clínica e subclínica. Portanto, a prevenção destas doenças também é muito mais eficiente do que o tratamento.
Assuntos
Feminino , Animais , Gravidez , Lactente , Bovinos , Desempenho Acadêmico/análise , Desempenho Acadêmico/prevenção & controle , Endometriose/prevenção & controle , Endometriose/veterinária , Fertilidade , Leite/provisão & distribuiçãoRESUMO
Dairy cows have shown a steep increase in milk production, which is unfortunately characterized by a dramatic decline in reproductive performance. In these modern high yielding cows authors worldwide mention a higher number of puerperal problems, such as retained placenta, acute metritis and abnormal vaginal discharge. All of which are known to negatively influence reproductive capacity of these animals. Cows affected by retained placenta and/or acute metritis are furthermore at a significantly higher risk of other typical dairy cow diseases such as acetonaemia, displaced abomasum and cystic ovarian disease. It is therefore important that the management of the cows during the transition period should be optimized in order to prevent cows from these disease complexes. Furthermore, during the immediate postpartum period, cows should be looked after properly in order to identify animals that are affected by clinical uterine disease as soon as possible so that treatment should not be delayed. High numbers of cows are furthermore reported to suffer from clinical and subclinical endometritis, both giving rise to significant problems to get in calf. At the moment, there is a lot of debate concerning both the diagnosis as well as the first choice treatment of cows suffering from clinical and subclinical endometritis. Therefore, also for these diseases prevention is far more effective than treatment.(AU)
Vacas de leite tem mostrado um aumento marcante na produção de leite, que infelizmente é caracterizado por um declínio dramático no desempenho reprodutor. Com relação a estas vacas de leite modernas de alto rendimento autores ao redor do mundo mencionam diversos problemas de puerpério, como placenta retida, metrite aguda e descarga vaginal anormal. Todos estes conhecidamente afetam a capacidade reprodutora destes animais. Vacas afetadas por placenta retida e/ou metrite aguda estão também em maior risco de outros tipos de doenças de vacas de leite como acetonaemia, abomaso deslocado e doença de ovário cístico. É importante, portanto, que o gerenciamento de vacas durante o período de transição deve ser otimizado para prevenir vacas contra estas doenças complexas. Além disto, durante o período pós parto imediato deve haver o cuidado apropriado das vacas para identificar animais afetados por doença uterina clínica assim que possível para não atrasar o tratamento. Um alto número de vacas sofrem de endometriose clínica e subclínica, ambos levando a problemas significativos para reproduzir. No momento há muito debate com relação ao diagnóstico e também ao primeiro tratamento de vacas sofrendo de endometriose clínica e subclínica. Portanto, a prevenção destas doenças também é muito mais eficiente do que o tratamento.(AU)
Assuntos
Animais , Feminino , Gravidez , Lactente , Bovinos , Endometriose/prevenção & controle , Endometriose/veterinária , Desempenho Acadêmico/análise , Desempenho Acadêmico/prevenção & controle , Fertilidade , Leite/provisão & distribuiçãoRESUMO
Mesenchymal stem cells (MSCs), because of their immunomodulation and trophic activities, in addition to their capacity to regenerate damaged tissues, have potential for treatment of many diseases. The success of stem cell therapies depends, in part, on the method of cell delivery, which should provide wide cell distribution and homing in to injured sites. The objective of the present study was to developing a novel strategy for delivery of MSCs into the uterus of mares with endometrosis (degenerative alteration of uterine glands and surrounding stroma). Endometrosis was confirmed in all mares (N = 6) used in this study. To trace multipotent equine adipose tissue-derived MSCs (eAT-MSCs) in endometrial tissue, before transplantation, cells were stained with a fluorescent dye. During a synchronized estrus, the eAT-MSCs (2 × 10(7) diluted in 20 mL of sodium chloride 0.9%) were inoculated into uterus using a simple technique, similar to artificial insemination (AI) in mares. At 7 and 21 days after transplantation, homing of fluorescently labeled eAT-MSCs was observed by confocal microscopy of uterine biopsies collected from the uterine body and in both uterine horns, including glandular and periglandular spaces, in three of four treated mares. Herein, we propose a new method of MSCs delivery in uterus of mares with endometrosis, which was minimally invasive and technically simple.
Assuntos
Endometriose/veterinária , Doenças dos Cavalos/terapia , Cavalos , Transplante de Células-Tronco Mesenquimais/veterinária , Útero/transplante , Animais , Movimento Celular , Endometriose/patologia , Endometriose/terapia , Feminino , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco MesenquimaisRESUMO
The present investigation was intended to show a different immunohistochemical profile of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 in bovine uteri with adenomyosis during follicular phase. Uterine samples of 32 cows in reproductive age were taken from the medial third of one of the uterine horns and grouped according to the adenomyosis degree (superficial and deep). Tissue sections (4 µm) were incubated overnight at 4°C with monoclonal antibody for matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2. Staining intensities were evaluated in the luminal epithelium, ectopic and dystopic endometrial tissue (stroma, capillaries and glands), endometrial-myometrial border, myometrium, myometrial vessels (middle tunic and endothelium). The matrix metalloproteinase-2 expression was higher for deep adenomyosis samples, showing a differential mean reactivity in superficial endometrium, myometrial vessels, myometrium adjacent to adenomyotic focus and endometrial-myometrial border (P < 0.05). Moreover, matrix metalloproteinase-2 expression was higher in deep adenomyosis samples than that of Tissue inhibitor metalloproteinase-2 in almost all uterine structures analyzed (except for the endometrial and myometrial vessels and endometrial-myometrial border). The opposite was observed in the follicular phase, for both normal specimens and with superficial adenomyosis, where Tissue inhibitor metalloproteinase-2 expression was higher than that of matrix metalloproteinase-2. In conclusion, a differential pattern of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 was observed in cow uteri with adenomyosis.
Assuntos
Doenças dos Bovinos/enzimologia , Endometriose/veterinária , Endométrio/patologia , Metaloproteinase 2 da Matriz/metabolismo , Miométrio/patologia , Inibidor Tecidual de Metaloproteinase-2/metabolismo , Animais , Bovinos , Doenças dos Bovinos/metabolismo , Doenças dos Bovinos/patologia , Endometriose/enzimologia , Endometriose/metabolismo , Endometriose/patologia , Endométrio/metabolismo , Feminino , Fase Folicular , Imuno-Histoquímica , Miométrio/metabolismoRESUMO
Relacionaram-se as características da adenomiose com as fases do ciclo estral em 61 peças de úteros de bovinos colhidas em matadouros. A adenomiose foi classificada em superficial e profunda. A fase do ciclo estral foi estimada pela morfologia, pela coloração e pela vascularização do corpo lúteo e presença ou não de folículos ovarianos maiores que 8mm. Os animais que estavam em anestro (n=11) apresentaram a menor ocorrência de adenomiose (8,2 por cento), e os que estavam na fase lútea média (n=21), a maior (31,0 por cento). Nas fases lútea inicial (n=13) e folicular (n=16) as ocorrências foram semelhantes, 18,0 e 22,9 por cento, respectivamente. A maior porcentagem de adenomiose profunda ocorreu nas fases lútea inicial e média, 45,0 e 47,4 por cento, respectivamente, e durante o anestro e a fase folicular foram de 20,0 e 14,3 por cento, respectivamente. Os resultados sugerem que a fase do ciclo estral influencia na ocorrência de adenomiose e no grau de infiltração miometrial das glândulas endometriais
The relationship of the adenomyosis characteristics and the phases of the estrus cycle in 61 cows bovine's uteruses collected in slaughterhouses was studied. The adenomyoses were classified as superficial and deep. The morphology, staining and vascularization of the corpus luteum and the presence or not of larger ovarian follicles than 8mm helped to estimate tthe estrus cycle. The cows in anestrus (n=11) showed the least occurrence of adenomyosis (8.2 percent) and the animals in the medium luteal phase (n=21) the largest one(31,0 percent). In the initial luteal phase (n=13) and the follicular phase (n=16) the occurrences of adenomyosis were similar and equals to 18.0 and 22.9 percent, respectively. The largests percentage of deep adenomyosis were found in the initial and in the medium luteal phases, 45.0 and 47.4 percent, respectively, and during the anestrus and the follicular phase they were 20.0 and 14.3 percent, respectively. The data suggest that the cycle phase influences in adenomyosis occurrence and in the degree of miometrial infiltration of the endometrial glands
Assuntos
Animais , Feminino , Gravidez , Bovinos , Bovinos , Ciclo Estral/fisiologia , Doenças Uterinas/veterinária , Endometriose/classificação , Endometriose/patologia , Endometriose/veterinária , Útero/anatomia & histologia , Útero/fisiopatologia , MatadourosRESUMO
Relacionaram-se as características da adenomiose com as fases do ciclo estral em 61 peças de úteros de bovinos colhidas em matadouros. A adenomiose foi classificada em superficial e profunda. A fase do ciclo estral foi estimada pela morfologia, pela coloração e pela vascularização do corpo lúteo e presença ou não de folículos ovarianos maiores que 8mm. Os animais que estavam em anestro (n=11) apresentaram a menor ocorrência de adenomiose (8,2 por cento), e os que estavam na fase lútea média (n=21), a maior (31,0 por cento). Nas fases lútea inicial (n=13) e folicular (n=16) as ocorrências foram semelhantes, 18,0 e 22,9 por cento, respectivamente. A maior porcentagem de adenomiose profunda ocorreu nas fases lútea inicial e média, 45,0 e 47,4 por cento, respectivamente, e durante o anestro e a fase folicular foram de 20,0 e 14,3 por cento, respectivamente. Os resultados sugerem que a fase do ciclo estral influencia na ocorrência de adenomiose e no grau de infiltração miometrial das glândulas endometriais(AU)
The relationship of the adenomyosis characteristics and the phases of the estrus cycle in 61 cows bovine's uteruses collected in slaughterhouses was studied. The adenomyoses were classified as superficial and deep. The morphology, staining and vascularization of the corpus luteum and the presence or not of larger ovarian follicles than 8mm helped to estimate tthe estrus cycle. The cows in anestrus (n=11) showed the least occurrence of adenomyosis (8.2 percent) and the animals in the medium luteal phase (n=21) the largest one(31,0 percent). In the initial luteal phase (n=13) and the follicular phase (n=16) the occurrences of adenomyosis were similar and equals to 18.0 and 22.9 percent, respectively. The largests percentage of deep adenomyosis were found in the initial and in the medium luteal phases, 45.0 and 47.4 percent, respectively, and during the anestrus and the follicular phase they were 20.0 and 14.3 percent, respectively. The data suggest that the cycle phase influences in adenomyosis occurrence and in the degree of miometrial infiltration of the endometrial glands(AU)