RESUMO
Mutations in the CLCN1 gene are the primary cause of non-dystrophic Hereditary Myotonia in several animal species. However, there are no reports of Hereditary Myotonia in pigs to date. Therefore, the objective of the present study was to characterize the clinical and molecular findings of Hereditary Myotonia in an inbred pedigree. The clinical, electromyographic, histopathological, and molecular findings were evaluated. Clinically affected pigs presented non-dystrophic recessive Hereditary Myotonia. Nucleotide sequence analysis of the entire coding region of the CLCN1 gene revealed the absence of the exons 15 and 16 in myotonic animals. Analysis of the genomic region flanking the deletion unveiled a large intragenic deletion of 4,165 nucleotides. Interestingly, non-related, non-myotonic pigs expressed transcriptional levels of an alternate transcript (i.e., X2) that was identical to the deleted X1 transcript of myotonic pigs. All myotonic pigs and their progenitors were homozygous recessive and heterozygous, respectively, for the 4,165-nucleotide deletion. This is the first study reporting Hereditary Myotonia in pigs and characterizing its clinical and molecular findings. Moreover, to the best of our knowledge, Hereditary Myotonia has never been associated with a genomic deletion in the CLCN1 gene in any other species.
Assuntos
Canais de Cloreto/genética , Miotonia Congênita/veterinária , Deleção de Sequência , Doenças dos Suínos/genética , Animais , Sequência de Bases , Éxons , Feminino , Heterozigoto , Homozigoto , Masculino , Miotonia Congênita/genética , Linhagem , Suínos , Doenças dos Suínos/congênitoRESUMO
Congenital tremor in pigs involves several etiologies, including pestivirus, which may cause neurological injuries in different animal species. To evaluate whether bovine viral diarrhea virus (BVDV), an important pestivirus, is one of the etiological agents of congenital tremor in swine, gilts and the fetuses were challenged at 45 days of gestation with BVDV-2. Four pregnant gilts were inoculated oronasally, four gilts underwent fetal intrauterine inoculation, and two gilts constituted the control group. Antibody titers were determined by virus neutralization (VN), and viral RNA was detected by RT-PCR. Blood samples were collected from all gilts and piglets born to obtain whole blood and serum for analysis. One third of the neonates were euthanized at three days old, and samples of the encephalon, brain stem and spinal cord were collected for anatomopathological evaluation and viral RNA detection. The piglets that remained alive were clinically evaluated every day, and blood sampling was performed regularly for 35 days. The piglets from gilts in both inoculation treatment groups showed no clinical neurological signs and were born with no viral RNA in their blood and organs. Piglets born from oronasally inoculated gilts did not present antibodies against BVDV-2 at birth, although they were acquired by passive maternal transfer. In contrast, intrauterine-inoculated piglets were born with high antibody titers (80 to 640) against the agent, which remained high until the end of the experimental period. Microscopically, no noticeable changes were observed. Macroscopically, 29.5% of the total piglets euthanized, from both inoculation groups, were born with a low cerebellar:brain ratio. Nevertheless, some piglets had a high cerebellar:brain ratio, indicating the need for standardizing this value. Thus, it was concluded that BVDV is not an etiological agent for congenital swine tremor.
Assuntos
Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia , Cerebelo/anormalidades , Malformações do Sistema Nervoso/veterinária , Doenças dos Suínos/congênito , Tremor/congênito , Tremor/etiologia , Animais , Animais Lactentes , Anticorpos Antivirais/sangue , Encéfalo/virologia , Bovinos , Cerebelo/virologia , Deficiências do Desenvolvimento/virologia , Vírus da Diarreia Viral Bovina Tipo 2/genética , Vírus da Diarreia Viral Bovina Tipo 2/isolamento & purificação , Feminino , Feto/virologia , Malformações do Sistema Nervoso/virologia , Placenta/virologia , Gravidez , Complicações Infecciosas na Gravidez/virologia , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real , Suínos , Doenças dos Suínos/virologia , Tremor/virologiaRESUMO
Recently, a putative new pestivirus species, provisionally named as Atypical Porcine Pestivirus (APPV), was associated with the congenital tremor in piglets in North America and consequently in Europe and Asia. The present research aimed to describe the detection and characterization of APPV employing NS5B gene partial sequencing, gross pathology and histologic examination of piglets displaying congenital tremor from two different farms of Southern Brazil. No gross lesions were observed, and the histological findings revealed moderate vacuolization of the white matter of the cerebellum. RT-PCR followed by DNA sequencing and a phylogenetic analysis confirmed the presence of APPV in samples from the two farms, which the samples were distinct in nature. Phylogenetic reconstruction reinforced the high genetic variability within the APPVs previously reported. This is the first report of APPV in South America suggesting that this new group of viruses may be widespread in swine herds in other countries as it is in Brazil.
Assuntos
Pestivirus/classificação , Doenças dos Suínos/virologia , Tremor/veterinária , Animais , Brasil/epidemiologia , Feminino , Pestivirus/genética , Pestivirus/isolamento & purificação , Filogenia , Análise de Sequência de DNA/veterinária , Suínos , Doenças dos Suínos/congênito , Doenças dos Suínos/epidemiologia , Tremor/congênito , Tremor/epidemiologia , Tremor/virologiaRESUMO
Em 51 leitöes natimortos examinados, encontraram-se 21,56 por cento de malformaçöes congênitas. As mais numerosas ocorreram no sistema urinário, 13,72 por cento, seguidas das digestivas, 5,88 por cento e da circulatória, 1,96 por cento. As alteraçöes das vias urinárias foram classificadas como hidroureter, 7,84 por cento, e hidronefrose, 5,88 por cento. O segundo grupo de alteraçöes englobou os cistos hepáticos e pancreáticos, com 5,88 por cento de ocorrências. A anomalia circulatória, 1,96 por cento, foi diagnosticada como persistência do arco aórtico direito. Observou-se presença concomitante de mais de uma alteraçäo em um mesmo indivíduo