RESUMO
Amoebiasis is a parasitic disease caused by Entamoeba histolytica (E. histolytica), an extracellular enteric protozoan. This infection mainly affects people from developing countries with limited hygiene conditions, where it is endemic. Infective cysts are transmitted by the fecal-oral route, excysting in the terminal ileum and producing invasive trophozoites (amoebae). E. histolytica mainly lives in the large intestine without causing symptoms; however, possibly as a result of so far unknown signals, the amoebae invade the mucosa and epithelium causing intestinal amoebiasis. E. histolytica possesses different mechanisms of pathogenicity for the adherence to the intestinal epithelium and for degrading extracellular matrix proteins, producing tissue lesions that progress to abscesses and a host acute inflammatory response. Much information has been obtained regarding the virulence factors, metabolism, mechanisms of pathogenicity, and the host immune response against this parasite; in addition, alternative treatments to metronidazole are continually emerging. An accesible and low-cost diagnostic method that can distinguish E. histolytica from the most nonpathogenic amoebae and an effective vaccine are necessary for protecting against amoebiasis. However, research about the disease and its prevention has been a challenge due to the relationship between E. histolytica and the host during the distinct stages of the disease is multifaceted. In this review, we analyze the interaction between the parasite, the human host, and the colon microbiota or pathogenic microorganisms, which together give rise to intestinal amoebiasis.
Assuntos
Amebíase/parasitologia , Países em Desenvolvimento , Disenteria Amebiana/parasitologia , Intestinos/parasitologia , Saúde Pública , Amebíase/tratamento farmacológico , Amebíase/epidemiologia , Animais , Antiprotozoários/uso terapêutico , Disenteria Amebiana/epidemiologia , Entamoeba histolytica/imunologia , Entamoeba histolytica/patogenicidade , Fezes/parasitologia , Microbioma Gastrointestinal , Interações Hospedeiro-Patógeno , Humanos , Intestinos/microbiologia , Metronidazol/uso terapêutico , Camundongos , VirulênciaRESUMO
Background: Intestinal amebiasis with morphological lesions and clinical manifestations is uncommon in dogs. The disease is caused by the protozoan Entamoeba histolytica, which is commonly observed in its natural hosts, humans and some nonhuman primates. It is occasionally found in the company of animals, usually associated with contact with infected humans. Thus, the objective here is to describe a case of necro-hemorrhagic colitis caused by E. histolytica in a dog infected with the canine distemper virus, in order to characterize the epidemiological and clinicopathological aspects of the disease. Case: An adult, mixed-breed bitch displaying anorexia and ataxia was referred to the veterinary hospital for treatment. Clinical evaluation showed a cachectic animal with 12% dehydration, ocular discharge, and bilateral purulent nasal discharge. A clinical diagnosis of distemper was made, and treatment was instituted. The dogs signs progressed to walking in circles, aimlessly, with lethargy and blindness. After three days of the onset of neurological signs, the dog developed diarrhea with hematochezia. With no improvement noted, we elected to euthanize the dog. At necropsy, edema was present in subcutaneous tissues, and the lungs had yellow areas in the cranio-ventral portions, which the court was flowing purulent discharge. In the large intestine, segmental distention of the distal portion of the descending colon was observed. The segment was approximately 15 cm in length and consisted of irregular reddish areas. There was also slight thickening of the wall with edematous mucosa containing blood clots, fibrin, and multiple areas of ulceration. Microscopically showed necro-hemorrhagic colitis associated with rounded structures, approximately 15 µm in size, containing abundant eosinophilic cytoplasm that was slightly granular or vacuolated. They also contained nuclei and nucleoli that were central or slightly eccentric [ ](AU)
Assuntos
Animais , Cães , Disenteria Amebiana/epidemiologia , Disenteria Amebiana/patologia , Disenteria Amebiana/veterinária , Entamoeba histolytica/isolamento & purificação , Coinfecção/veterinária , Hemorragia Gastrointestinal/veterinária , Imuno-Histoquímica/veterináriaRESUMO
Background: Intestinal amebiasis with morphological lesions and clinical manifestations is uncommon in dogs. The disease is caused by the protozoan Entamoeba histolytica, which is commonly observed in its natural hosts, humans and some nonhuman primates. It is occasionally found in the company of animals, usually associated with contact with infected humans. Thus, the objective here is to describe a case of necro-hemorrhagic colitis caused by E. histolytica in a dog infected with the canine distemper virus, in order to characterize the epidemiological and clinicopathological aspects of the disease. Case: An adult, mixed-breed bitch displaying anorexia and ataxia was referred to the veterinary hospital for treatment. Clinical evaluation showed a cachectic animal with 12% dehydration, ocular discharge, and bilateral purulent nasal discharge. A clinical diagnosis of distemper was made, and treatment was instituted. The dogs signs progressed to walking in circles, aimlessly, with lethargy and blindness. After three days of the onset of neurological signs, the dog developed diarrhea with hematochezia. With no improvement noted, we elected to euthanize the dog. At necropsy, edema was present in subcutaneous tissues, and the lungs had yellow areas in the cranio-ventral portions, which the court was flowing purulent discharge. In the large intestine, segmental distention of the distal portion of the descending colon was observed. The segment was approximately 15 cm in length and consisted of irregular reddish areas. There was also slight thickening of the wall with edematous mucosa containing blood clots, fibrin, and multiple areas of ulceration. Microscopically showed necro-hemorrhagic colitis associated with rounded structures, approximately 15 µm in size, containing abundant eosinophilic cytoplasm that was slightly granular or vacuolated. They also contained nuclei and nucleoli that were central or slightly eccentric [ ]
Assuntos
Animais , Cães , Coinfecção/veterinária , Disenteria Amebiana/epidemiologia , Disenteria Amebiana/patologia , Disenteria Amebiana/veterinária , Entamoeba histolytica/isolamento & purificação , Hemorragia Gastrointestinal/veterinária , Imuno-Histoquímica/veterináriaRESUMO
The frequency and severity of dysentery and hepatic abscess during the colonial and republican era in Chile are reviewed. The amebian etiology was confirmed in both clinical entities. Also, Miguel Claro Vásquez, physician and later priest and bishop of the Catholic Church, was distinguished for his contribution to hepatic abscess surgery.
Assuntos
Surtos de Doenças/história , Disenteria Amebiana/história , Abscesso Hepático Amebiano/história , Chile/epidemiologia , Disenteria Amebiana/epidemiologia , História do Século XVII , História do Século XVIII , História do Século XIX , Humanos , Abscesso Hepático Amebiano/epidemiologiaRESUMO
BACKGROUND: Epidemiological and microbiologic studies suggest that enteropathogenic microorganisms play a substantial role in the clinical initiation and relapses of inflammatory bowel disease. AIM: To explore the prevalence of intestinal protozoa in patients with ulcerative colitis (UC) and its impact on clinical disease course. METHODS: A total of 215 patients with definitive diagnosis of UC were studied. Fresh feces samples taken from all UC patients were examined immediately using trichrome-staining methods. RESULTS: A total of 103 female and 112 male UC patients were analyzed. The mean age at diagnosis was 30.5 +/- 10.8 years. The prevalence of overall parasitic infections was 24% and distributed as follows: Blastocystis hominis in 22 patients (10%), Endolimax nana in 19 cases (9%), and Entamoebahistolytica in 11 cases (5%). A significantly increased frequency of protozoa infection was found in those patients with persistent activity and intermittent activity as compared to active than inactive group (p = 1 x 10(-7), OR 13.05, 95% CI 4.28-42.56, and p = 0.003, OR 1.42-14.47, respectively). Interestingly, this association remained significant when we compared the persistent activity group versus intermittent activity group (p = 0.003, OR 2.97, 95% CI 1.35-6.59). Subgroup analysis showed no association between protozoa infection (E. histolytica, B. hominis, and E. nana) and other clinical variables such as gender, extent of disease, extraintestinal complications, medical treatment and grade of disease activity. CONCLUSION: The prevalence of intestinal protozoa infections in Mexican UC patients was 24% and these microorganisms could be a contributing cause of persistent activity despite medical treatment in our population.
Assuntos
Amebíase/epidemiologia , Infecções por Blastocystis/epidemiologia , Colite Ulcerativa/parasitologia , Amebíase/parasitologia , Animais , Infecções por Blastocystis/parasitologia , Blastocystis hominis/isolamento & purificação , Distribuição de Qui-Quadrado , Colite Ulcerativa/epidemiologia , Progressão da Doença , Disenteria Amebiana/epidemiologia , Disenteria Amebiana/parasitologia , Endolimax/isolamento & purificação , Entamoeba histolytica/isolamento & purificação , Fezes/parasitologia , Feminino , Humanos , Masculino , México/epidemiologia , Pessoa de Meia-Idade , PrevalênciaRESUMO
The frequency and severity of dy sentery and hepatic abscess during the colonial and republican era in Chile are reviewed. The amebian etiology was confirmed in both clinical entities. Also, Miguel Claro Vásquez, physician and later priest and bishop of the Catholic Church, was distinguished for his contribution to hepatic abscess surgery.
En esta comunicación se destaca la frecuencia y gravedad en Chile, durante la época colonial y republicana, de dos entidades clínicas: disentería y absceso hepático, comprobándose finalmente la etiología amebiana en ambos procesos. Además se distingue al doctor Miguel Claro Vásquez, médico, después sacerdote y obispo de la Iglesia Católica, por su aporte a la cirugía del absceso del hígado.
Assuntos
História do Século XVII , História do Século XVIII , História do Século XIX , Humanos , Surtos de Doenças/história , Disenteria Amebiana/história , Abscesso Hepático Amebiano/história , Chile/epidemiologia , Disenteria Amebiana/epidemiologia , Abscesso Hepático Amebiano/epidemiologiaRESUMO
En esta comunicación se destaca la frecuencia y gravedad en Chile, durante la época colonial y republicana, de dos entidades clínicas: disentería y absceso hepático, comprobándose finalmente la etiología amebiana en ambos procesos. Además se distingue al doctor Miguel Claro Vásquez, médico, después sacerdote y obispo de la Iglesia Católica, por su aporte a la cirugía del absceso del hígado.(AU)
Assuntos
História do Século XVII , História do Século XVIII , História do Século XIX , Disenteria Amebiana/história , Abscesso Hepático Amebiano/história , História da Medicina , Disenteria Amebiana/epidemiologia , Abscesso Hepático Amebiano/epidemiologia , Retrato , Chile/epidemiologiaRESUMO
While Giardia duodenalis infection has been consistently associated with nutrient malabsorption and stunting in children, the effects of other protozoans on nutritional status or gastrointestinal morbidity are less clear. We sought to determine whether infection with common intestinal protozoans including Giardia duodenalis, Entamoeba coli and Blastocystis hominis was associated with anthropometric and micronutrient status, gastrointestinal symptoms, visits to the doctor or school absenteeism in children 5-12 years of age from Bogotá, Colombia. We obtained stool samples from 442 children enrolled in primary schools in 2006 and examined the presence of intestinal protozoans in relation to height, body mass index, plasma concentrations of vitamins A and B12, ferritin and zinc and erythrocyte folate. In addition, we examined the associations between protozoan infections and the incidence of common gastrointestinal symptoms, which were registered prospectively in morbidity diaries. The prevalence rates of G. duodenalis, E. coli and B. hominis infection were 6.3, 23.1 and 22.4%, respectively. Giardia infection was associated with lower height-for-age z-score (p = 0.04), whereas E. coli infection was associated with low erythrocyte folate (p = 0.04), and B. hominis infection was related to higher vitamin A levels (p = 0.05). Infection with E. coli was also associated with a significantly higher incidence of fever but fewer visits to the doctor, while B. hominis infection was associated with significantly less diarrhea, diarrhea with vomiting, doctor visits and school absenteeism. In conclusion, G. duodenalis and E. coli infections were associated with indicators of poor nutritional status in this population, while B. hominis was related to apparently decreased morbidity.
Assuntos
Disenteria Amebiana/complicações , Giardíase/complicações , Micronutrientes/sangue , Estado Nutricional , Absenteísmo , Blastocystis hominis/isolamento & purificação , Índice de Massa Corporal , Criança , Pré-Escolar , Colômbia/epidemiologia , Disenteria Amebiana/epidemiologia , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/complicações , Infecções por Escherichia coli/epidemiologia , Fezes/parasitologia , Feminino , Trato Gastrointestinal/fisiopatologia , Giardia/isolamento & purificação , Giardíase/epidemiologia , Humanos , Incidência , Masculino , Morbidade , Prevalência , Estudos Prospectivos , Instituições Acadêmicas , Fatores SocioeconômicosRESUMO
INTRODUCTION: There are morphologically identical amebaes, but with differences that can distinguish them; one as pathogenic: Entamoeba histolytica, and the other: Entamoeba dispar, as inoffensive. That brought the new hypothesis that many of the cases treated as amebiasis, weren't so. OBJECTIVE: To identify E. hystolitica in patients with dysentery, supposed to be caused by amebae. METHODS: Transversal and observational study performed between March 2005 and November 2007 in the city of Santa Fe, Argentina. Stools from children aged 2 months to 15 years-old with dysentery and direct exams with E. hystolitica/ dispar, were studied with ELISA to detect the adhesin of E. histolytica (adhesin Eh). Permanent stains for amebae were done as well as stool cultures. Clinical data were charted. RESULTS: 75 children were studied; 35 were male and 40, female, with a median age of 3 years-old. All of them presented diarrhea with leucocyte, 73% macroscopic blood on stool and 27% detectable on the microscope. Elisa Eh was positive in 21; 3 cases had hematophagous trophozoites. In 15 stool cultures were found: S. flexneri S2 type in 5 cases. Other parasites: 6 (Blastocystis homini 5). In 54 adhesin Eh was negative, 19% of the coulouring detected E. dispar. From 44 stool cultures: S. flexneri S2 type was detected in 13, Shigella sp in 1, C jejuni 5, other: 3. Other parasites: 12 (Blastocystis homini 9). CONCLUSION: In this group of children with "amebic dysentery", half of them developed invasive bacteriae and only 28% had E. histolytica on stools; that means that the prevalence of positive cases in the population could be 18% to 38% [CI 95% (0.179; 0.381)].
Assuntos
Disenteria Amebiana , Disenteria/epidemiologia , Disenteria/parasitologia , Adolescente , Argentina/epidemiologia , Criança , Pré-Escolar , Diagnóstico Diferencial , Disenteria Amebiana/complicações , Disenteria Amebiana/diagnóstico , Disenteria Amebiana/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Lactente , Masculino , PrevalênciaRESUMO
An epidemiological and molecular study on E. histolytica and E. dispar was carried out in 428 patients with gastrointestinal symptomatology of diarrhea from different health centers in Cumana, Sucre state. The samples were processed through: direct examination with 0.85% physiological saline solution, temporal lugol staining, trichromic staining and the Ritchie method of concentration; a sucrose gradient was used for cyst isolation. The small subunit of the 16S RNA was amplified by nested, multiplex PCR for the molecular detection. The E. histolytica/E. dispar prevalences according to the direct, Ritchie and trichromic staining methods were 20.09, 13.79 and 12.15%, respectively; while prevalences according to PCR for E. histolytica and E. dispar were 6.31% and 4.44%, respectively, also detecting four cases of mixed infection. Sequencing of the amplified fragments of E. histolytica showed 100% homology with the sequences with strains from Merida (Venezuela), USA, Brazil, Mexico and GenBank. The infections by E. histolytica and E. dispar were statistically associated with age but not with sex. The presence of mucus, blood and abdominal pain were only associated to E. histolytica infection. The moderate prevalence of E. histolytica shows the endemic status of this population and warns about the potential problem as a morbidity and mortality in Sucre state. The frequency of E. dispar in this population suggests the existence of an overestimation problem in the diagnosis of amoebiasis with its clinical and epidemiological implications, and shows the poor knowledge about the true prevalences of this protozoan. The PCR allowed for the differential identification of E. histolytica and E. dispar, as well as the presence of mixed infections, making a great tool for epidemiological amoebiasis studies.
Assuntos
Disenteria Amebiana/parasitologia , Entamoeba histolytica/isolamento & purificação , Entamoeba/isolamento & purificação , Adolescente , Adulto , Idoso , Animais , Sequência de Bases , Criança , Pré-Escolar , Diarreia/etiologia , Diarreia/parasitologia , Disenteria Amebiana/epidemiologia , Doenças Endêmicas , Entamoeba/genética , Entamoeba histolytica/genética , Feminino , Helmintíase/epidemiologia , Helmintíase/parasitologia , Humanos , Lactente , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Prevalência , Infecções por Protozoários/epidemiologia , Infecções por Protozoários/parasitologia , Ribotipagem , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Especificidade da Espécie , Venezuela/epidemiologiaRESUMO
The frequency of Entamoeba histolytica and Entamoeba dispar infection was analyzed in a rural community in the state of Morelos, Mexico, using polymerase chain reaction (PCR). Sociodemographic variables as risk factors for the infection were assessed. Results highlighted the number of individuals with intestinal parasites (43.1%) in the community, indicating extensive fecalism. A high frequency of E. histolytica asymptomatic infection, higher than E. dispar infection (13.8% versus 9.6%), was detected by PCR. Anti-amebic antibody levels (IgG) in serum and saliva (IgA) samples were not associated with E. histolytica intestinal infection. These findings suggest a predominant distribution of E. histolytica strains of low invasive potential in this community.
Assuntos
Disenteria Amebiana/epidemiologia , Entamoeba histolytica , Abscesso Hepático Amebiano/epidemiologia , Animais , Anticorpos Antiprotozoários/sangue , Sequência de Bases , Primers do DNA , Entamoeba histolytica/genética , Entamoeba histolytica/isolamento & purificação , Família , Fezes/parasitologia , Humanos , México/epidemiologia , Reação em Cadeia da Polimerase , Fatores de Risco , População Rural , Abastecimento de ÁguaRESUMO
The genetic variability of Entamoeba dispar strains was investigated in 39 positive isolates on a survey of 1783 individuals from two different cities of Northeast Brazil (Recife and Macaparana) using two polymorphic species-specific loci (loci 1-2 and 5-6). A combinatory clustering analysis revealed no geographical correlation and remarkable genetic polymorphism among all the isolates examined. Nevertheless, a comparison of the frequency of eight individual PCR products, shared by both Recife and Macaparana populations, for the two loci, showed that only one product of locus 5-6 was significantly different between the two cities. These results suggested that the Macaparana population is infected by similar strains and that locus 5-6 shows potential in assaying questions related to the molecular epidemiology of this region.
Assuntos
Disenteria Amebiana/parasitologia , Entamoeba/genética , Enteropatias Parasitárias/parasitologia , Adolescente , Animais , Brasil/epidemiologia , Criança , Pré-Escolar , Análise por Conglomerados , DNA de Protozoário/química , DNA de Protozoário/genética , Disenteria Amebiana/epidemiologia , Entamoeba/isolamento & purificação , Fezes/parasitologia , Variação Genética , Humanos , Enteropatias Parasitárias/epidemiologia , Reação em Cadeia da Polimerase , Áreas de Pobreza , População Rural , População UrbanaRESUMO
OBJECTIVE: Determine the prevalence of intestinal parasitosis between January 1, 1997 and December 31, 1999 in the microbiology laboratory of the Fort de France University Hospital. METHOD: Retrospective study of the results of 4684 parasitological examinations of stools performed in 2704 patients between January 1, 1997 and December 31, 1999 in this laboratory. RESULTS: This survey showed the high prevalence of anguillulosis, found in 51.69% of infested patients and in 4.56% of the population studied, the ever high prevalence of non or scarcely pathogenic amoebas (Endolimax nanus, Dientamoeba fragilis, Entamoeba coli) found in 27.19% of infested patients and 1.88% of the population studied, together with that of hookworms (12.80% of infested patients and 1.13% of the population), and the persistence of lambliasis. This study also revealed the presence of cryptosporidies (7 cases) and microsporidies (4 cases) in the patients infected by the human immunodeficiency virus. DISCUSSION: These results confirm the trend of the past twenty Years and the results of surveys initiated by the national statistics board (INSERM) in 1978, 1988 and 1995-1995, with the regression of bilharziosis and the persistence of anguillulosis and hookworms. CONCLUSION: The improvement in living conditions and hygiene, the combined efforts of the health care workers and Authorities in Martinique over the past 30 Years in the fight against parasites have led to a great reduction in the prevalence of classical intestinal parasitosis. However, new parasites associated with HIV infection have appeared.
Assuntos
Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/parasitologia , Centros Médicos Acadêmicos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Doenças Transmissíveis Emergentes/epidemiologia , Doenças Transmissíveis Emergentes/parasitologia , Criptosporidiose/epidemiologia , Criptosporidiose/parasitologia , Disenteria Amebiana/epidemiologia , Disenteria Amebiana/parasitologia , Fezes/parasitologia , Feminino , Giardíase/epidemiologia , Giardíase/parasitologia , Infecções por Uncinaria/epidemiologia , Infecções por Uncinaria/parasitologia , Humanos , Lactente , Enteropatias Parasitárias/prevenção & controle , Masculino , Martinica/epidemiologia , Microsporidiose/epidemiologia , Microsporidiose/parasitologia , Pessoa de Meia-Idade , Vigilância da População , Prevalência , Estudos Retrospectivos , Esquistossomose/epidemiologia , Esquistossomose/parasitologia , Estrongiloidíase/epidemiologia , Estrongiloidíase/parasitologiaRESUMO
Objetivo: determinar la prevalencia de parásitos intestinales y de Entamoeba histolytica mediante la prueba ELISA de adhesina en heces en asentamientos temporales post-terromoto. Diseño: estudio de prevalencia de punto. Lugar: ocho asentamientos temporales post-terremoto de la ciudad de Armenia. Población: muestra representativa de la población entre 4 y 65 años de edad de asentamientos, en total 169 personas. Mediciones: coproscópico y prueba ELISA para adhesina de Entamoeba histolytica en heces. Resultados: Endolimax nana fue el parásito comensal más prevalente con 66 por ciento, seguido por Entamoeba coli 26 por ciento y Lodamoeba buschtlii 10 por ciento. Giardia lamblia fue la especie patógena más prevalente con 38 por ciento, seguida de Ascaris lumbricoides 10 por ciento, Strongyloides stercoralis 2 por ciento, Trichuris trichura 1.7 por ciento, Hymenolepsis nana 0.6 por ciento (1/169). Los quistes de Entamoeba histolytica/dispar fueron detectados por microscopía en 17.5 por ciento y la prueba de adhesina fue positiva en sólo una de las 169 muestras estudiadas (0.6 por ciento). Conclusiones: este estudio señala que E. histolytica es poco frecuente en asentamientos temporales post-terremoto y la necesidad en Colombia de realizar pruebas específicas, con el fin de determinar la instauración de un tratamiento antiamibiano, el cual no se puede basar en el resultado de la microscopía óptica dada la alta frecuencia de amibas no patógenas
Assuntos
Desastres Naturais , Disenteria Amebiana/epidemiologia , Entamoeba histolytica/isolamento & purificação , Adesinas Bacterianas , Fezes/microbiologia , Ensaio de Imunoadsorção EnzimáticaRESUMO
We investigated the risk of severe inflammatory diarrhoea associated with geohelminth parasites in 1746 children attending a rural hospital in Ecuador from December 2000 to July 2002. Infections with geohelminths were strongly protective against the risk of severe inflammatory diarrhoea. Our findings suggest that geohelminth infections have important protective effects against enteroinvasive infections in young children.
Assuntos
Diarreia/epidemiologia , Helmintíase/epidemiologia , Animais , Estudos de Casos e Controles , Criança , Pré-Escolar , Diarreia/parasitologia , Disenteria Amebiana/epidemiologia , Equador/epidemiologia , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Razão de Chances , Prevalência , Estudos Retrospectivos , Fatores de Risco , Saúde da População Rural , Tricuríase/epidemiologia , TrichurisRESUMO
In a slum community in northeastern Brazil 20% of a sample population was colonized with Entamoeba histolytica or Entamoeba dispar and 10.6% was colonized with E. histolytica alone. No correlation between seropositivity for anti-Ga1NAc lectin antibody and colonization was found. These results suggest that colonization does not necessarily produce immunity to reinfection.
Assuntos
Disenteria Amebiana/epidemiologia , Entamoeba histolytica , Entamoeba/isolamento & purificação , Entamebíase/epidemiologia , Acetilgalactosamina , Adolescente , Adulto , Animais , Anticorpos Antiprotozoários/sangue , Brasil/epidemiologia , Criança , Pré-Escolar , Disenteria Amebiana/classificação , Entamoeba histolytica/isolamento & purificação , Entamebíase/classificação , Fezes/parasitologia , Humanos , Lactente , Lectinas/imunologia , Pessoa de Meia-Idade , Prevalência , Infecções por Protozoários/diagnóstico , Infecções por Protozoários/epidemiologia , Proteínas de Protozoários/imunologiaRESUMO
Por la unidad epidemiológica que tiene el desarrollo de procedimientos que permitan estudios confiables de prevalencia de amebiosis intestinal se estudió la eficacia de ENZYMEBA, ensayo inmunoenzimático para la detección en heces de Entamoeba histolytica. ENZYMEBA, mediante el empleo de una sola muestra por individuo participante en el estudio, demostró infección por E. histolytica en 27 de los 686 casos estudiados. La observación microscópica de heces sólo igualó los resultados de ENZYMEBA cuando fueron analizados los resultados de ambos procedimientos sobre 3 muestras de cada uno de los individuos en el estudio. Por su alta sensibilidad, requerir de una sola muestra por persona encuestada y ser un inmunoensayo realizable en condiciones mínimas de laboratorio, consideramos que ENZYMEBA es una herramienta muy útil para estudios de prevalencia de amebiosis intestinal