RESUMO

This study described the hepatobiliary anatomopathological lesions associated with trematode Platynosomum illiciens parasitism in Neotropical primates kept in captivity. In the evaluated organs, we observed portal fibrosis, biliary epithelial hyperplasia, and inflammatory reaction with a predominance of lymphocytes and plasmocytes, and in some cases infiltration of eosinophils and neutrophils.

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RESUMO

The Dicrocoeliidae is a highly diverse and broadly distributed family of digeneans typically parasitic in the gall bladder and liver of their tetrapod hosts. So far, no study has reported dicrocoeliids, or any digeneans, from the ancient marsupial family Caenolestidae. Herein, we describe a new genus of dicrocoeliids (Otongatrema n. gen.) from Tate's shrew opossum Caenolestes fuliginosus and a new species of Metadelphis (Metadelphis cesartapiai n. sp.) from a phyllostomid bat Anoura peruana collected in Ecuador. Otongatrema can be readily distinguished from the morphologically closest dicrocoeliid genera Concinnum, Conspicuum and Canaania based on the position of the genital pore, distribution/position of the uterus and extent of vitellarium. Metadelphis cesartapiai can be easily differentiated from other Metadelphis spp. based on a combination of morphological characters including body shape and size, distribution of vitellarium, shape of the gonads as well as size of suckers and cirrus sac. In addition, we used newly generated partial sequences of the nuclear 28S rRNA gene and mitochondrial cox1 genes to examine phylogenetic affinities of the new taxa within the Dicrocoeliidae. Both the 28S and cox1 phylogenies confidently positioned Otongatrema as a sister/basal group to all other dicrocoeliids sequenced so far. The phylogenetic position of Otongatrema may be explained by a close co-evolutionary relationship with Caenolestidae, one of the most basal and most ancient groups of marsupials. In addition, our 28S phylogeny provides evidence that the complete or partial loss of intestinal structures has likely occurred independently at least 3 times in the evolutionary history of the Dicrocoeliidae.

Assuntos

RESUMO

Metadelphis tkachi n. sp. is described based on material from the gall-bladder of Molossus molossus (Pallas) (Chiroptera: Molossidae) in the Brazilian eastern Amazon. The main differentiating characters of the new species include lobed, tandem testes and elongated clusters of vitelline follicles commencing at the level of the seminal receptacle. The new species can be distinguished from Metadelphis lenti (Santos & Gibson, 1998) and Metadelphis apharyngotrema (Marshall & Miller, 1979) by the shape and position of the testes; from Metadelphis evandroi Travassos, 1944 by having tandem, lobed testes and shorter clusters of vitelline follicles; from Metadelphis compactus Travassos, 1955 by having tandem testes and more elongated clusters of vitelline follicles, and from Metadelphis alverangai Travassos, 1955 by having smaller testes and body, and vitellarium with large follicles. New morphological data are reported for M. lenti based on light and scanning electron microscopy; intraspecific variation was observed.

Assuntos

RESUMO

The enzyme adenosine deaminase (ADA) is critical for modulating the immune system, and in the presence of zinc, its activity is catalyzed. The aim of this study was to evaluate the ADA activity in pancreas of cattle naturally infected by Eurytrema coelomaticum in relation to the results of zinc levels, pathological findings and parasite load. For this study 51 slaughtered cattle were used. The animals were divided into two groups: Group A consisting of animals naturally infected by E. coelomaticum (n=33) and Group B of uninfected animals (n=18). Blood and pancreas were collected of each animal for analysis of zinc and ADA, respectively. Infected cattle showed a reduction on seric levels of zinc, and decreased ADA activity in the pancreas (P>0.05). A positive correlation between zinc levels and ADA activity was observed. Thus, high parasite load and severity of histopathologic lesions affect the ADA activity in pancreas, as well as the zinc levels in serum of infected animals (negative correlation between these variables). Therefore, we can conclude that cattle infected by E. coelomaticum have low ADA activity in pancreas, which can be directly related to zinc reduction, responsible for ADA activation and catalyzes.

Assuntos

RESUMO

This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.

Assuntos

RESUMO

This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.

Este estudo avaliou a ocorrência de Eurytrema spp. em bovinos por meio da análise da sequência parcial do gene 18S rRNA. Trematódeos procedentes de 44 pâncreas bovinos foram coletados e classificados com base em aspectos morfológicos típicos. A técnica de PCR e a análise da sequência de nucleotídeos dos produtos amplificados confirmaram que os trematódeos classificados como Eurytrema coelomaticum eram filogeneticamente distintos daqueles identificados como E. pancreaticum. Os resultados deste estudo representam a primeira caracterização molecular de E. coelomaticum nas Américas e disponibiliza um método eficiente para diferenciar trematódeos digenéticos em animais domésticos.

Assuntos

RESUMO

This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.

Este estudo avaliou a ocorrência de Eurytrema spp. em bovinos por meio da análise da sequência parcial do gene 18S rRNA. Trematódeos procedentes de 44 pâncreas bovinos foram coletados e classificados com base em aspectos morfológicos típicos. A técnica de PCR e a análise da sequência de nucleotídeos dos produtos amplificados confirmaram que os trematódeos classificados como Eurytrema coelomaticum eram filogeneticamente distintos daqueles identificados como E. pancreaticum. Os resultados deste estudo representam a primeira caracterização molecular de E. coelomaticum nas Américas e disponibiliza um método eficiente para diferenciar trematódeos digenéticos em animais domésticos.

Assuntos

RESUMO

Durante o estudo dos metazoários parasitos de Paroaria dominicana Linnaeus, 1758 (Passeriformes), quatro infrapopulações de Platynosomum illiciens (Brown, 1901) (Trematoda) foram coletadas nos ductos hepáticos, com intensidades parasitárias de 10, 11, 18 e 36 espécimes respectivamente. Diferenças entre os valores morfométricos médios e o número total de ovos das infrapopulações foram estatisticamente testadas. O peso do fígado não se correlacionou ao peso das aves, ao comprimento do corpo das aves e à abundância parasitária. Foram detectadas correlações positivas entre o tamanho das infrapopulações e o número de ovos produzidos e entre o tamanho do corpo dos parasitos e o tamanho dos ovos. Correlações negativas foram observadas entre a intensidade parasitária e o peso do fígado; entre o tamanho das infrapopulações e o tamanho dos indivíduos dessas infrapopulações; entre o número e o tamanho dos ovos e entre o tamanho do corpo dos parasitos e o número de ovos. Os resultados indicaram que as infrapopulações maiores se estabeleceram em aves com fígados menores e que quanto maiores as infrapopulações foram observados menor tamanho corporal e menor tamanho dos ovos, os que se apresentaram em maior quantidade.

During the study of the metazoan parasites of Paroaria dominicana Linnaeus, 1758 (Passeriformes), four infrapopulations of Platynosomum illiciens (Brown, 1901) (Trematoda), parasitic in the liver ducts were collected, with intensity of infection 10, 11, 18 and 36 specimens respectively. Differences between the means of morphometrical values and the total number of eggs were statistically tested. The liver weigh were not significantly correlated with avian total weigh and length, and with the total parasitic abundance. Positive correlations were observed between the size of infrapopulations and the total number of eggs, and between parasite body size and the eggs. size. Negative correlations were observed between parasitic intensity and liver weigh; between the size of infrapopulations and the parasite body size; between total number and the size of eggs and between parasite body size and total number of eggs. These results suggests that host with smaller liver had greater infrapopulations. These greater infrapopulations showed smaller parasite body size and higher size and number of eggs.

Assuntos

RESUMO

During the study of the metazoan parasites of Paroaria dominicana Linnaeus, 1758 (Passeriformes), four infrapopulations of Platynosomum illiciens (Brown, 1901) (Trematoda), parasitic in the liver ducts were collected, with intensity of infection 10, 11, 18 and 36 specimens respectively. Differences between the means of morphometrical values and the total number of eggs were statistically tested. The liver weigh were not significantly correlated with avian total weigh and length, and with the total parasitic abundance. Positive correlations were observed between the size of infrapopulations and the total number of eggs, and between parasite body size and the eggs. size. Negative correlations were observed between parasitic intensity and liver weigh; between the size of infrapopulations and the parasite body size; between total number and the size of eggs and between parasite body size and total number of eggs. These results suggests that host with smaller liver had greater infrapopulations. These greater infrapopulations showed smaller parasite body size and higher size and number of eggs.

Assuntos