RESUMO
BACKGROUND: The immunogenic potential of dentin has been reported through dentin-reactive autoantibodies detection in human and animal model. This study aimed to investigate the formation and diagnostic value of immune complexes formation after autoantibodies production, and soluble dentin antigens levels associated to root resorption, in the course of orthodontic tooth movement, in rat experimental model. METHODS: Forty Wistar rats (n = 8 for each group) were submitted to orthodontic tooth movement, in which the maxillary right first molar was mesially moved by applying of 55 g of force for 3, 7, 14, or 21 days. Untreated group was used as control. Circulating autoantibodies to rat dentinal extract, immune complexes, and soluble dentinal antigen levels were determined by immunoenzyme assays. Additionally, dentinal antigens were analyzed by immunoblot. RESULTS: Higher serum dentin-reactive IgG and immune complex levels were detected in the 14- and 21-day groups (p < 0.05 and p < 0.001 respectively) but not in circulating dentinal antigen levels (p > 0.05), as compared to the control group. Reactivity was found to dentinal components with molecular mass (MM) ~120 and ~150 kDa, by immunoblot. CONCLUSION: This work represents the first evidence of immune complexes formation and circulating soluble dentin antigens associated to root resorption in orthodontic tooth movement. Immune complexes formation could be used to early diagnosis of external root resorption.
Assuntos
Complexo Antígeno-Anticorpo/sangue , Dentina/imunologia , Técnicas de Movimentação Dentária/métodos , Animais , Complexo Antígeno-Anticorpo/imunologia , Autoanticorpos/biossíntese , Imunoglobulina G/sangue , Incisivo/patologia , Masculino , Maxila/patologia , Modelos Animais , Dente Molar/patologia , Coelhos , Ratos , Ratos Wistar , Reabsorção da Raiz/patologia , Estresse MecânicoRESUMO
Anti-dentin autoantibodies are associated with inflammatory root resorption in permanent teeth and are modulated by dental trauma and orthodontic force. However, it is not known whether deciduous tooth trauma can stimulate the development of a humoral immune response against dentin. The aim of this study was to evaluate the levels of salivary SIgA reactivity against human dentin extract in young adults with a history of trauma in the primary dentition. A sample of 78 patients, aged 18 to 25, who had completed an early childhood (0 to 5 years old) caries prevention program years earlier at the Universidade Estadual de Londrina Pediatric Clinic, underwent radiographic examination and salivary sampling. Anti-dentin SIgA levels were analyzed by immunoenzymatic assay and Western blotting. Although dental trauma to deciduous teeth had occurred in 34 (43.6%) of the patients, no differences in SIgA levels were detected between individuals who had experienced trauma and those who had not (p > 0.05). Multivariate regression analysis showed no association between dental trauma and SIgA levels (p > 0.05). Patients with a history of deciduous trauma presented low levels of anti-dentin antibodies, associated with orthodontic root resorption (p < 0.05). Western blot analysis showed that salivary antibodies recognized a single band of approximately 45 kDa in dentin extract. We concluded that salivary SIgA recognizes a specific component of the dentin matrix and that anti-dentin antibodies were not triggered by trauma to primary teeth. However, trauma to deciduous teeth may down-modulate SIgA in response to orthodontic root response.
Assuntos
Dentina/imunologia , Imunoglobulina A Secretora/imunologia , Reabsorção da Raiz/imunologia , Reabsorção de Dente , Dente Decíduo/imunologia , Adolescente , Adulto , Pré-Escolar , Dentina/lesões , Feminino , Humanos , Imunoglobulina A Secretora/análise , Lactente , Recém-Nascido , Masculino , Reabsorção da Raiz/etiologia , Saliva/imunologia , Dente Decíduo/lesões , Adulto JovemRESUMO
Anti-dentin autoantibodies are associated with inflammatory root resorption in permanent teeth and are modulated by dental trauma and orthodontic force. However, it is not known whether deciduous tooth trauma can stimulate the development of a humoral immune response against dentin. The aim of this study was to evaluate the levels of salivary SIgA reactivity against human dentin extract in young adults with a history of trauma in the primary dentition. A sample of 78 patients, aged 18 to 25, who had completed an early childhood (0 to 5 years old) caries prevention program years earlier at the Universidade Estadual de LondrinaPediatric Clinic, underwent radiographic examination and salivary sampling. Anti-dentin SIgA levels were analyzed by immunoenzymatic assay and Western blotting. Although dental trauma to deciduous teeth had occurred in 34 (43.6%) of the patients, no differences in SIgA levels were detected between individuals who had experienced trauma and those who had not (p > 0.05). Multivariate regression analysis showed no association between dental trauma and SIgA levels (p > 0.05). Patients with a history of deciduous trauma presented low levels of anti-dentin antibodies, associated with orthodontic root resorption (p < 0.05). Western blot analysis showed that salivary antibodies recognized a single band of approximately 45 kDa in dentin extract. We concluded that salivary SIgA recognizes a specific component of the dentin matrix and that anti-dentin antibodies were not triggered by trauma to primary teeth. However, trauma to deciduous teeth may down-modulate SIgA in response to orthodontic root response.
Assuntos
Adolescente , Adulto , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Adulto Jovem , Dentina/imunologia , Imunoglobulina A Secretora/imunologia , Reabsorção da Raiz/imunologia , Reabsorção de Dente , Dente Decíduo/imunologia , Dentina/lesões , Imunoglobulina A Secretora/análise , Reabsorção da Raiz/etiologia , Saliva/imunologia , Dente Decíduo/lesõesRESUMO
Replacement dental resorption may be a consequence of trauma and may cause dental transplants or reimplants to fail. Previously, we demonstrated the participation of the immunopathological response in inflammatory dental resorption. The induction mechanisms of the two types of dental resorption are well known to be different. The aim of the present study was to observe the immune response of patients who suffered dental trauma with subsequent replacement dental resorption. Four patients with replacement radicular resorption and four healthy individuals with no evidence of radicular resorption participated in the study. The results of ELISA demonstrated that serum from patients with replacement dental resorption contained larger amounts of IgG and smaller amounts of IgM anti-total human-dentin extract and anti-fractions of extract than did serum from control individuals. These results signal the hypothesis that dentin is immunogenic and the serological profile of patients with replacement dental resorption may be identified through biochemical analysis of their blood. Precise screening by this method may allow early diagnosis of dental resorption before it becomes visible radiographically.
Assuntos
Formação de Anticorpos/imunologia , Reabsorção da Raiz/imunologia , Traumatismos Dentários/imunologia , Adolescente , Adulto , Estudos de Casos e Controles , Dentina/imunologia , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Masculino , Proteínas/imunologia , Reabsorção da Raiz/sangue , Traumatismos Dentários/sangueRESUMO
OBJECTIVE: The aim of this study was to characterize the effects of dentin extracts on cytokine, chemokine and nitric oxide (NO) production by primary rat bone cells. STUDY DESIGN: Osteoblastic bone marrow cultures were exposed to particulate (D-part), non-particulate (D-n-part) and demineralized dentin extracts and evaluated for proliferative activity, cell morphology, alkaline phosphatase activity and bone-like nodule formation. Cytokine production was assessed by enzyme-linked immunosorbent assay and NO release by the Griess method. RESULTS: The dentin extracts did not affect osteoblast numbering. Conversely, they up regulated in a dose-dependent manner the production by the osteoblasts of the pro-inflammatory interleukin-1beta (IL-1beta), tumor necrosis factor-alpha, IL-6, cytokine-induced neutrophil chemoattractant-1, and of the anti-inflammatory cytokine, IL-10. The NO production was stimulated only by D-n-part. CONCLUSION: These results demonstrate that dentin induces the production of inflammatory cytokines by osteoblasts and suggest that pro-resorptive pathways might be stimulated when dentin molecules come into contact with bone cells during pathological processes associated with dentin and bone matrix dissolution.