RESUMO
OBJECTIVE: Vascular endothelial growth factor A (VEGFA) and its receptors are essential proteins for the angiogenic activity of dental pulp. Angiogenesis fundamentally provides oxygen and nutrients to cells for root formation and defence mechanisms. The angiogenic potential of dental pulp should be understood and considered for the conservative and regenerative endodontics. The purpose of this research was to measure the VEGFA expression and its receptors such as vascular endothelial growth factor receptors 1, -2 (VEGFR1, VEGFR2) and Neuropilin 1 (NRP1) in human dental pulp from molars with immature and mature apexes. METHODS: VEGFA system mRNAs expressions were assessed in dental pulp obtained from freshly extracted human third molars divided into immature (n=8) and mature (n=8) apexes. RNAs were extracted from the samples. Each sample's cDNA was synthetized and the target genes VEGFA, VEGFR1, VEGFR2, NRP1 expression profiles obtained by RT2-PCR. Analysis was based on the Student's t-test comparing the replicate 2-ΔCt values for each gene. P values of <0.05 were considered significant. RESULTS: In teeth with mature apexes, VEGFA (P=0.0002), NRP1 (P=0.0001), VEGFR1 (P=0.0057) and VEGFR2 (P=0.018259) significantly increased statistically with respect to the immature apexes group. CONCLUSION: Within the limitation of the present investigation, it can be concluded that the angiogenic process seems to be a physiological process in the dental pulp due to the studied angiogenic growth factor are expressed in both immature and mature dental pulps. VEGFA and its receptors are expressed significantly higher in mature apex teeth than immature apex teeth.
Assuntos
Polpa Dentária , Fator A de Crescimento do Endotélio Vascular , Polpa Dentária/metabolismo , Expressão Gênica , Humanos , Dente Molar , Dente Serotino/metabolismo , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
The aim of the study was to evaluate the effect of experimental composites containing dicalcium phosphate dihydrate (DCPD) on remineralization of enamel lesions. Five resin-based composites containing equal parts (in mols) of bisphenol-A glycidyl dimethacrylate (BisGMA), triethylene glycol dimethacrylate (TEGDMA), and 60 vol % of fillers were manipulated. Filler phase was constituted by silanized barium glass and 0, 10, or 20 vol % of DPCD particles, either functionalized (F) or nonfunctionalized (NF) with TEGDMA. Artificial subsurface lesions were produced in human enamel fragments and divided according to the resin composite applied on the lesion (no DCPD, 20% NF, 20% F, 10% NF, 10% F) plus a group without composite build-up (nontreated, NT). Fragments were exposed to 16 days of pH cycling. Specimens were evaluated using transverse microradiography (TMR). Calcium and phosphate concentrations in pH-cycling solutions were determined by spectrophotometry. TMR and ionic concentrations were analyzed using one-way ANOVA/Tukey and Kruskal-Wallis/Dunn test, respectively (alpha: 0.05). All composite groups showed enamel remineralization (3%-23%). Higher mineral recovery in the middle (7%-11%) and bottom (2%-7%) thirds of the lesion was observed in groups with DCPD-containing composites compared to the "no DCPD" group (middle: 1%, bottom: -3%). Lesion depth was significantly reduced in groups using DCPD-containing composites compared to NT group. No noticeable increase in calcium and phosphate ions was observed in the pH-cycling solutions due to the presence of DCPD in the composites. In conclusion, composites with DCPD fractions as low as 10%, regardless of functionalization, were able to promote mineral recovery and reduce lesion depth of enamel lesions. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1542-1550, 2019.
Assuntos
Fosfatos de Cálcio/química , Resinas Compostas/química , Cárie Dentária/terapia , Esmalte Dentário/química , Metacrilatos/química , Polietilenoglicóis/química , Ácidos Polimetacrílicos/química , Compostos de Bário/química , Bis-Fenol A-Glicidil Metacrilato/química , Humanos , Íons/química , Teste de Materiais , Microrradiografia , Minerais/química , Dente Serotino/metabolismo , Silanos/química , Dióxido de Silício/química , Propriedades de Superfície , Remineralização DentáriaRESUMO
Teneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed in the central nervous system during development. All teneurins contain a bioactive peptide in their carboxyl terminal named teneurin C-terminal associated peptide (TCAP). The present study analyzed the detailed distribution of teneurin-2-like immunoreactive (Ten-2-LI) cells in developing and mature rat molar teeth, as well as in mature human dental pulps. Ten-2 and TCAP-2 genic expressions were also evaluated in rat and human dental pulps. Finally, Ten-2-LI cells were analyzed during the repair process after dentin-pulp complex injury in rat lower molar teeth. For this, histological sections of rat molar teeth and human dental pulps were submitted to immunohistochemical techniques, while total RNA from developing rat teeth and mature human dental pulps were submitted to conventional RT-PCR. Ten-2-LI cells were evident in the initial bell stage of rat molar teeth development, especially in ectomesenchymal cells of the dental papilla. Ten-2-LI odontoblasts showed strong immunoreactivity in rat and human mature teeth. Ten-2 and TCAP-2 genic expressions were confirmed in rat and human dental pulps. Dentin-pulp complex injury resulted in a decrease of Ten-2-LI odontoblasts after traumatic injury. Interestingly, Ten-2-LI cells were also evident in the pulp cell-rich zone in all postoperative days. In conclusion, Ten-2-LI presence in rat and human odontoblasts was demonstrated for the first time and Ten-2/TCAP-2 genic expressions were confirmed in rat and human dental pulps. Furthermore, it was revealed that Ten-2-LI rat odontoblasts can be modulated during the regenerative process.
Assuntos
Proteínas do Tecido Nervoso/metabolismo , Odontoblastos/metabolismo , Animais , Células Cultivadas , Polpa Dentária/citologia , Polpa Dentária/metabolismo , Polpa Dentária/patologia , Dentina/metabolismo , Dentina/patologia , Feminino , Humanos , Imuno-Histoquímica , Masculino , Microscopia Confocal , Dente Molar/crescimento & desenvolvimento , Dente Molar/metabolismo , Dente Molar/patologia , Dente Serotino/citologia , Dente Serotino/metabolismo , Dente Serotino/patologia , Proteínas do Tecido Nervoso/genética , Odontoblastos/citologia , Ratos , Ratos WistarRESUMO
La proteína C reactiva CRP (por sus siglas en inglés) es una proteína de fase aguda que se utiliza para el seguimiento de enfermedades inflamatorias tales como artritis reumatoidea, lupus eritematoso o vasculitis y procesos infecciosos tales como sepsis y septicemia; así como también, para evaluar la eficacia de las drogas antiinflamatorias y antimicrobianas indicadas en el tratamiento de estas patologías. Igualmente se ha asociado a daño tisular en diversas especialidades quirúrgicas. El objetivo de este estudio fue relacionar los niveles plasmáticos de CRP con la infección y el edema posterior a la cirugía de los terceros molares. A tal efecto se evaluaron 60 pacientes, distribuidos en 3 grupos A, B y C bajo antibioticoterapia profiláctica con Clindamicina (A: dosis única de 600 mg, B: 300 mg c/6h por 5 días y C: Placebo) y terapia analgésica y antiinflamatoria (Ibuprofeno 400mg c/6h por 3 días). A quienes se tomaron muestras de sangre antes y a las 72 horas de la odontectomía de los terceros molares y fotografías digitales para calcular el área de inflamación. No se demostró la relación de los niveles de CRP con infección ya que ningún paciente presentó proceso infeccioso pero si se demostró la relación cualitativa (sensibilidad) de CRP y cuantitativa mediante correlación de Spearman (p<0,05) ya que mientras mayor fue el área de la inflamación, mayores fueron los niveles plasmáticos de CRP.
The C reactive protein (CRP) is an unspecific acute phase reaction used for the follow-up of such inflammatory diseases such as rheumatoid arthritis, lupus, or vasculitis and such infectious processes like sepsis; as well as also, to evaluate the efficiency of the anti-inflammatory and antimicrobial drugs indicated in the treatment of this pathologies. Equally it has associated to tissue damage in diverse surgical specialties. The aim of this study was to evaluate the relation between CRP levels as indicator of postoperative infection and edema after third molar surgery. We evaluated 60 patients distributed in three groups A, B and C under antibiotic prophylaxis with Clindamycin (A: single dosis 600 mgs, B: 300 mgs each 6/h by 5 days and C: placebo) and analgesic and anti-inflammatory therapy with Ibuprofen 400 mg. each 6/h by 3 days. Who were taken blood samples to measure the CRP before and 72 hours after surgery and digital photographs to calculate the edema area. We did not demonstrated relation between CRP and infection because no one patient was infected in any group but we demonstrated (By Searman (p<0,05) the value of CRP as indicator of edema in the third molar surgery.
Assuntos
Humanos , Masculino , Feminino , Anti-Inflamatórios , Infecção da Ferida Cirúrgica , Proteína C , Dente Serotino/cirurgia , Dente Serotino/metabolismo , Anti-Infecciosos , Dente Molar , Cirurgia BucalRESUMO
O propósito deste estudo foi avaliar o efeito do tempo de contaminação salivar (TCS) e de recondicionamento ácido (TRA) na resistência da união ao cisalhamento (RC) do selante Fluroshield. Foram selecionados 45 terceiros molares. Os dentes foram seccionados no sentido mésio-distal e suas superfícies foram lixadas até a obtenção de uma área plana em esmalte. Em seguida, as amostras foram distribuídas aleatoriamente em 9 grupos (n=10), em função do TCS e TRA (segundos), respectivamente: G1 - controle; G2 - 30 e 0; G3 - 60 e 0; G4 - 30 e 2; G5 - 30 e 5; G6 - 30 e 15; G7 - 60 e 2; G8 - 60 e 5; G9 - 60 e 15. Em seguida, foi realizada a aplicação do selante de acordo com as instruções do fabricante. Os corpos-de-prova obtidos foram armazenados em água destilada a 37ºC por 72 horas, e então submetidos ao ensaio de cisalhamento à velocidade de 0,5mm/min. Os resultados foram submetidos à análise de variância (ANOVA), e indicaram que não houve diferença estatística significativa (p>0,05) entre todos os grupos. Observou-se que: 1 - quanto maior o TCS, menores os valores de RC (MPa); 2- quanto maior o TRA, maior o valor de RC (MPa). Pôde-se concluir que houve uma tendência para o menor TCS (30s) e os maiores TRA (5 e 15s) em retornar aos valores de RC do grupo controle.