RESUMO

Promoters of genes encoding superoxide dismutase (sodA) and peptide methionine sulfoxide reductase (msrA) from Cory-nebacterium glutamicum were cloned and sequenced. Promoter region analysis of sodA-msrA was unable to identify putative sites of fixed eventual regulators except for possible sites of fixed OxyR and integra-tion host factor. A study of the regulation of these genes was performed using the lacZ gene of Escherichia coli as a reporter placed under the control of sequences downstream of sodA and msrA. In silico analysis was used to identify regulators in the genome of C. glutamicum, which revealed the absence of homologs of soxRS and arcA and the presence of inactive oxyR and putative candidates of the homologs of ahpC, ohrR, integration host factor, furA, IdeR, diphtheria toxin repressor, and mntR.

Assuntos

Corynebacterium glutamicum/genética , Regulação Bacteriana da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Metionina Sulfóxido Redutases/genética , Estresse Oxidativo/fisiologia , Superóxido Dismutase/genética , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/genética , Corynebacterium glutamicum/metabolismo , Corynebacterium glutamicum/efeitos da radiação , Metionina Sulfóxido Redutases/biossíntese , Estresse Oxidativo/genética , Regiões Promotoras Genéticas , Estresse Fisiológico , Superóxido Dismutase/biossíntese