RESUMO
A cartilage matrix glycoprotein (CMGP), previously identified in human and bovine vitreous, now has been found in the vitreous body of rabbits aged 1-22 months by immunohistochemical techniques. Epithelial cells of the inner layer of the ciliary epithelium contain material that has immunologic cross-reactivity with a specific antibody to CMGP. These cells also secrete glycoproteins, as determined by autoradiography after intravitreal injection of [3H]fucose. Approximately 14 bands, representing intrinsic glycoproteins containing fucose residues, can be identified in fluorograms of SDS-polyacrylamide gels of vitreous bodies from 6- and 22-month-old rabbits. Fluorograms of gels of samples of vitreous and ciliary bodies from several time points after intravitreal injection of [3H]fucose reveal at least seven comigrating protein bands and also demonstrate turnover of the labeled ciliary body glycoproteins. These results suggest that the inner layer of the ciliary epithelium is the source of the glycoproteins of the vitreous body and that these glycoproteins undergo turnover, probably throughout the entire life of the animals.
Assuntos
Proteínas do Olho/análise , Corpo Vítreo/análise , Animais , Antígenos/análise , Autorradiografia , Corpo Ciliar/análise , Epitélio/análise , Feminino , Técnicas Imunoenzimáticas , Masculino , CoelhosRESUMO
The lens of the eye possesses a capsule which is a greatly hypertrophied basement membrane. To investigate the synthesis of glycoproteins destined for this capsule, 3H-fucose was injected into the vitreous body of intact rats weighing approximately 200 gm. The animals were killed from 10 min to 14.5 months later, and their lenses were processed for electron microscope radioautography. At 10 min after injection, more than 58% of the silver grains were localized to the Golgi apparatus of the lens epithelial cells. By day 1, the heaviest sites of reaction were the plasma membrane (more than 50% of total label), the basal cytoplasm, and the adjacent lens capsule, where a heavy band of reaction was seen. The remainder of the capsule exhibited a lighter diffuse reaction. In the lens fibers, the label was at first localized to clusters of vesicles but then migrated to the plasma membrane and to the region of the capsule adjacent to the basal surface of these fibers. Light microscope radioautographs of the lens capsule at later time intervals revealed that by 1 month after injection the diffuse reaction had disappeared, and only the strongly labeled band remained. By 14.5 months after injection, this band had migrated partially across the lens capsule, but the capsule itself had increased considerably in thickness. On the other hand, the distance between the labeled band and the free edge of the capsule had decreased from that seen at the time of injection.
Assuntos
Glicoproteínas/biossíntese , Cápsula do Cristalino/metabolismo , Cristalino/metabolismo , Animais , Autorradiografia , Epitélio/análise , Epitélio/metabolismo , Fucose , Glicoproteínas/análise , Cápsula do Cristalino/análise , Cápsula do Cristalino/citologia , Masculino , Proteínas de Membrana/análise , Microscopia Eletrônica/métodos , Ratos , Ratos Endogâmicos , Corpo Vítreo/análiseAssuntos
Cobre/análise , Ferro/análise , Corpo Vítreo/análise , Zinco/análise , Humanos , Espectrofotometria Atômica , VitrectomiaAssuntos
Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Corpo Vítreo/análise , Mudanças Depois da Morte , Potássio/análise , Exames Médicos , Fatores de TempoRESUMO
Various body fluids have been used in the diagnosis of diseases. Rapid changes occur in the blood and cerebrospinal fluid after death. Vitreous humor, being well protected, shows a more gradual change. It has been found to be a useful adjunct in determining the time of death in an individual and in diagnosing certain antemortem clinical conditions (AU)