RESUMO
Degradation of yard wastes by Coprinus truncorum growing in a vertical aereated bioreactor or in flasks was studied. There was a constant decay of reducing sugars in the medium that avoided their accumulation and their possible repression of degradative enzymes. Endoxylanase activity at first showed a similar pattern in both culture conditions, with maximal activity on the 12th day, but flasks maintained a high activity thereafter. Flasks also showed a higher endoglucanase activity with a peak on the 18th day, whereas the maximal value in the bioreactor was reached on the 26th day. No Mn-peroxidase and only low values of laccase activity were found. The measurements of pH and soluble proteins during the incubation period were suitable indicators of the degradation process by C. truncorum.
Assuntos
Reatores Biológicos , Coprinus/fisiologia , Micologia/métodos , Plantas , Eliminação de Resíduos/métodos , Biodegradação Ambiental , Metabolismo dos Carboidratos , Celulase/metabolismo , Celulose/metabolismo , Coprinus/enzimologia , Coprinus/crescimento & desenvolvimento , Endo-1,4-beta-Xilanases , Proteínas Fúngicas/metabolismo , Resíduos de Alimentos , Concentração de Íons de Hidrogênio , Lacase , Lignina/metabolismo , Oxirredução , Oxirredutases/metabolismo , Peroxidases/metabolismo , Solubilidade , Temperatura , Xilosidases/metabolismoRESUMO
Degradation of yard wastes by Coprinus truncorum growing in a vertical aereated bioreactor or in flasks was studied. There was a constant decay of reducing sugars in the medium that avoided their accumulation and their possible repression of degradative enzymes. Endoxylanase activity at first showed a similar pattern in both culture conditions, with maximal activity on the 12th day, but flasks maintained a high activity thereafter. Flasks also showed a higher endoglucanase activity with a peak on the 18th day, whereas the maximal value in the bioreactor was reached on the 26th day. No Mn-peroxidase and only low values of laccase activity were found. The measurements of pH and soluble proteins during the incubation period were suitable indicators of the degradation process by C. truncorum.(AU)