RESUMO
Humans and animals can be exposed to different chemical forms of mercury (Hg) in the environment. For example, methylmercury (MeHg)-contaminated fish is part of the basic diet of the riparian population in the Brazilian Amazon Basin, which leads to high total blood and plasma Hg levels in people living therein. Hg induces toxic effects mainly through oxidative stress. Different compounds have been used to prevent the damage caused by MeHg-induced reactive oxygen species (ROS). This study aims to investigate the in vivo effects of sub-chronic exposure to low MeHg levels on the mitochondrial oxidative status and to evaluate the niacin protective effect against MeHg-induced oxidative stress. For this purpose, Male Wistar rats were divided into four groups: control group, treated with drinking water on a daily basis; group exposed to MeHg at a dose of 100 µg/kg/day; group that received niacin at a dose of 50 mg/kg/day in drinking water, with drinking water being administered by gavage; group that received niacin at a dose of 50 mg/kg/day in drinking water as well as MeHg at a dose of 100 µg/kg/day. After 12 weeks, the rats, which weighed 500-550 g, were sacrificed, and their liver mitochondria were isolated by standard differential centrifugation. Sub-chronic exposure to MeHg (100 µg/kg/day for 12 weeks) led to mitochondrial swelling (p < 0.05) and induced ROS overproduction as determined by increased DFCH oxidation (p < 0.05), increased gluthatione oxidation (p < 0.05), and reduced protein thiol content (p < 0.05). In contrast, niacin supplementation inhibited oxidative stress, which counteracted and minimized the toxic MeHg effects on mitochondria.
Assuntos
Compostos de Metilmercúrio/toxicidade , Mitocôndrias Hepáticas/efeitos dos fármacos , Niacina/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Animais , Masculino , Compostos de Metilmercúrio/administração & dosagem , Mitocôndrias Hepáticas/patologia , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/metabolismoRESUMO
BACKGROUND: Exposure to vinylcyclohexene (VCH) and methylmercury (MeHg+) can induce oxidative stress and gene modulation. Several studies have been evaluating the effects of VCH and MeHg+, but little is known about interactive effects between them. This work aimed to assess the exposure and co-exposure effects of MeHg+ and VCH on oxidative stress and gene modulation in Drosophila melanogaster. METHODS: Reactive species production, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were evaluated after exposure and co-exposure to VCH (1 mM) and MeHg+ (0.2 mM) for one or three days in the head and body (thorax and abdomen) of flies. The expression of genes related to redox state and inflammatory response was evaluated after exposure and co-exposure to VCH and MeHg+ for three days. RESULTS: Survival decreased only in flies co-exposed to VCH and MeHg+ for three days. All treatments increased total reactive species production after one day of exposure. However, no significant changes were observed in the head after three days of exposure. One day of exposure to VCH caused an increase in the head GST activity, whereas MeHg+ induced an increase after three days of exposure. Regarding the body, all treatments increased GST activity after one day of exposure, but only the flies exposed to MeHg+ presented an increase in GST activity after three days of exposure. Treatments did not alter AChE activity in the head. As for gene expression, there was a significant increase in the Relish transcription factor gene in the flies' body, but Nrf2, Keap1, Jafrac1, TrxR1, and NF-κß were not altered. CONCLUSION: The results suggest that exposure to VCH and MeHg+ induce oxidative stress and activation of an inflammatory response in fruit flies.
Assuntos
Cicloexenos/toxicidade , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/efeitos dos fármacos , Expressão Gênica/efeitos dos fármacos , Compostos de Metilmercúrio/toxicidade , Acetilcolinesterase/genética , Acetilcolinesterase/metabolismo , Animais , Cicloexenos/administração & dosagem , Relação Dose-Resposta a Droga , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Sinergismo Farmacológico , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Compostos de Metilmercúrio/administração & dosagem , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/genéticaRESUMO
Despite the vast distribution among tissues, the central nervous system (CNS) represents the main target of methylmercury (MeHg) toxicity. However, few studies have evaluated the effects of MeHg exposure on the CNS at equivalent doses to human environmental exposure. In our study, we evaluated the motor cortex, an important area of motor control, in adult rats chronically exposed to MeHg in a concentration equivalent to those found in fish-eating populations exposed to mercury (Hg). The parameters evaluated were total Hg accumulation, oxidative stress, tissue damage, and behavioral assessment in functional actions that involved this cortical region. Our results show in exposed animals a significantly greater level of Hg in the motor cortex; increase of nitrite levels and lipid peroxidation, associated with decreased antioxidant capacity against peroxyl radicals; reduction of neuronal and astrocyte density; and poor coordination and motor learning impairment. Our data showed that chronic exposure at low doses to MeHg is capable of promoting damages to the motor cortex of adult animals, with changes in oxidative biochemistry misbalance, neurodegeneration, and motor function impairment.
Assuntos
Compostos de Metilmercúrio/farmacologia , Córtex Motor/efeitos dos fármacos , Córtex Motor/fisiopatologia , Destreza Motora/efeitos dos fármacos , Degeneração Neural/induzido quimicamente , Degeneração Neural/patologia , Estresse Oxidativo/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Masculino , Compostos de Metilmercúrio/administração & dosagem , Córtex Motor/patologia , Ratos , Ratos WistarRESUMO
Methylmercury (MeHg) is a hazardous environmental pollutant, affecting Amazon basin communities by anthropogenic activities. The exact safe level of MeHg exposure is unclear, despite the efforts of health international societies to avoid mercury (Hg) poisoning. Central nervous system is severely impacted by Hg intoxication, reflecting on motor impairment. In addition, alcohol has been associated to an overall brain damage. According to lifestyle of Amazon riverside communities, alcohol intake occurs frequently. Thus, we investigated if continuous MeHg exposure at low doses during adolescence displays motor deficits (experiment 1). In the experiment 2, we examine if the co-intoxication (i.e. MeHg plus ethanol exposure) during adolescence intensify motor damage. In the experiment 1, Wistar adolescent rats (31 days old) received chronic exposure to low dose (CELD) of MeHg (40 µg/kg/day) for 35 days. For the experiment 2, five sessions of alcohol binge drinking paradigm (3ON-4OFF; 3.0 g/kg/day) were employed associated to MeHg intoxication. Motor behaviour was evaluated by the open field, pole test, beam walking and rotarod paradigms. CELDS of MeHg display motor function damage, related to hypoactivity, bradykinesia-like behaviour, coordination deficits and motor learning impairment. Co-intoxication of MeHg plus ethanol reduced cerebellar Hg content, however also resulted in motor behavioural impairment, as well as additive effects on bradykinesia and fine motor evaluation.
Assuntos
Intoxicação Alcoólica/fisiopatologia , Compostos de Metilmercúrio/toxicidade , Atividade Motora/efeitos dos fármacos , Adolescente , Animais , Consumo Excessivo de Bebidas Alcoólicas/fisiopatologia , Cerebelo/efeitos dos fármacos , Cerebelo/metabolismo , Poluentes Ambientais/toxicidade , Etanol/administração & dosagem , Etanol/toxicidade , Feminino , Humanos , Hipocinesia/induzido quimicamente , Mercúrio/administração & dosagem , Mercúrio/farmacocinética , Compostos de Metilmercúrio/administração & dosagem , Ratos Wistar , Testes de Toxicidade CrônicaRESUMO
Human exposure to relatively low levels of methylmercury is worrying, especially in terms of its genotoxicity. It is currently unknown as to whether exposure to low levels of mercury (below established limits) is safe. Genotoxicity was already shown in lymphocytes, but studies with cells of the CNS (as the main target organ) are scarce. Moreover, disturbances in the cell cycle and cellular proliferation have previously been observed in neuronal cells, but no data are presently available for glial cells. Interestingly, cells of glial origin accumulate higher concentrations of methylmercury than those of neuronal origin. Thus, the aim of this work was to analyze the possible genotoxicity and alterations in the cell cycle and cell proliferation of a glioma cell line (C6) exposed to a low, non-lethal and non-apoptotic methylmercury concentration. Biochemical (mitochondrial activity) and morphological (integrity of the membrane) assessments confirmed the absence of cell death after exposure to 3 µM methylmercury for 24 hours. Even without promoting cell death, this treatment significantly increased genotoxicity markers (DNA fragmentation, micronuclei, nucleoplasmic bridges and nuclear buds). Changes in the cell cycle profile (increased mitotic index and cell populations in the S and G2/M phases) were observed, suggesting arrest of the cycle. This delay in the cycle was followed, 24 hours after methylmercury withdrawal, by a decrease number of viable cells, reduced cellular confluence and increased doubling time of the culture. Our work demonstrates that exposure to a low sublethal concentration of MeHg considered relatively safe according to current limits promotes genotoxicity and disturbances in the proliferation of cells of glial origin with sustained consequences after methylmercury withdrawal. This fact becomes especially important, since this cellular type accumulates more methylmercury than neurons and displays a vital role protecting the CNS, especially in chronic intoxication with this heavy metal.
Assuntos
Compostos de Metilmercúrio/toxicidade , Mutagênicos/toxicidade , Animais , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Dano ao DNA , Humanos , Compostos de Metilmercúrio/administração & dosagem , Testes de Mutagenicidade , Mutagênicos/administração & dosagem , Neuroglia/efeitos dos fármacos , Neuroglia/metabolismo , Neuroglia/patologia , RatosRESUMO
Organoseleno-compounds have been investigated for its beneficial effects against methylmercury toxicity. In this way, diphenyl diselenide (PhSe)2 was demonstrated to decrease Hg accumulation in mice, protect against MeHg-induced mitochondrial dysfunction, and protect against the overall toxicity of this metal. In the present study we aimed to investigate if co-treatment with (PhSe)2 and MeHg could decrease accumulation of Hg in liver slices of rats. Rat liver slices were co-treated with (PhSe)2 (0.5; 5 µM) and/or MeHg (25 µM) for 30 min at 37 °C and Se and Hg levels were measured by inductively coupled plasma mass spectrometry (ICP-MS) in the slices homogenate, P1 fraction, mitochondria and incubation medium. Co-treatment with (PhSe)2 and MeHg did not significantly alter Se levels in any of the samples when compared with compounds alone. In addition, co-treatment with (PhSe)2 and MeHg did not decrease Hg levels in any of the samples tested, although, co-incubation significantly increased Hg levels in homogenate. We suggest here that (PhSe)2 could exert its previously demonstrated protective effects not by reducing MeHg levels, but forming a complex with MeHg avoiding it to bind to critical molecules in cell.
Assuntos
Derivados de Benzeno/farmacologia , Fígado/química , Fígado/efeitos dos fármacos , Mercúrio/análise , Compostos de Metilmercúrio/farmacologia , Compostos Organosselênicos/farmacologia , Selênio/análise , Animais , Derivados de Benzeno/administração & dosagem , Masculino , Espectrometria de Massas , Compostos de Metilmercúrio/administração & dosagem , Mitocôndrias Hepáticas/química , Mitocôndrias Hepáticas/efeitos dos fármacos , Mitocôndrias Hepáticas/metabolismo , Compostos Organosselênicos/administração & dosagem , Ratos , Ratos WistarRESUMO
Methylmercury (MeHg) is a highly toxic environmental contaminant that produces neurological and developmental impairments in animals and humans. Although its neurotoxic properties have been widely reported, the molecular mechanisms by which MeHg enters the cells and exerts toxicity are not yet completely understood. Taking into account that MeHg is found mostly bound to sulfhydryl-containing molecules such as cysteine in the environment and based on the fact that the MeHg-cysteine complex (MeHg-S-Cys) can be transported via the L-type neutral amino acid carrier transport (LAT) system, the potential beneficial effects of L-methionine (L-Met, a well known LAT substrate) against MeHg (administrated as MeHg-S-Cys)-induced neurotoxicity in mice were investigated. Mice were exposed to MeHg (daily subcutaneous injections of MeHg-S-Cys, 10 mg Hg/kg) and/or L-Met (daily intraperitoneal injections, 250 mg/kg) for 10 consecutive days. After treatments, the measured hallmarks of toxicity were mostly based on behavioral parameters related to motor performance, as well as biochemical parameters related to the cerebellar antioxidant glutathione (GSH) system. MeHg significantly decreased motor activity (open-field test) and impaired motor performance (rota-rod task) compared with controls, as well as producing disturbances in the cerebellar antioxidant GSH system. Interestingly, L-Met administration did not protect against MeHg-induced behavioral and cerebellar changes, but rather increased motor impairments in animals exposed to MeHg. In agreement with this observation, cerebellar levels of mercury (Hg) were higher in animals exposed to MeHg plus L-Met compared to those only exposed to MeHg. However, this event was not observed in kidney and liver. These results are the first to demonstrate that L-Met enhances cerebellar deposition of Hg in mice exposed to MeHg and that this higher deposition may be responsible for the greater motor impairment observed in mice simultaneously exposed to MeHg and L-Met.
Assuntos
Cerebelo/química , Cisteína/análogos & derivados , Poluentes Ambientais/toxicidade , Metionina/farmacologia , Compostos de Metilmercúrio/toxicidade , Atividade Motora/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Desempenho Psicomotor/efeitos dos fármacos , Animais , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Cerebelo/metabolismo , Cisteína/administração & dosagem , Cisteína/farmacocinética , Cisteína/toxicidade , Esquema de Medicação , Poluentes Ambientais/administração & dosagem , Poluentes Ambientais/farmacocinética , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Injeções Intraperitoneais , Masculino , Metionina/administração & dosagem , Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/farmacocinética , Camundongos , Fármacos Neuroprotetores/administração & dosagem , Distribuição AleatóriaRESUMO
Methylmercury (MeHg) is an environmental pollutant that is highly toxic to the central nervous system. As its effects on male reproductive system are poorly understood, this study was carried out to analyse the effects of MeHg on the rat prostate. To evaluate the MeHg toxicity on ventral prostate, three groups of adult male Wistar rats received oral doses of 0.5, 1.0 and 3.0 mg/kg MeHg, respectively, on a daily basis for 14 days. A fourth group was used as a control. The prostate weight was decreased in rats treated orally with 0.5 mg/kg MeHg compared to controls. Also, Hg concentration increased significantly in the prostate after treatments. There were reductions in serum testosterone levels and androgen receptor immunoreactivity in animals receiving 3.0 mg MeHg/kg. The stereological data showed changes in the prostatic epithelial, stromal and luminal compartments which varied according to the different doses. Histopathological alterations, such as chronic inflammation, stratified epithelial hyperplasia and epithelial inflammatory reactive atypia, were observed in the 0.5 mg/kg MeHg-treated group. Epithelial atrophy was observed in the 3.0 mg/kg MeHg-treated group. In conclusion, the MeHg affects prostatic homoeostasis resulting in histopathological changes that may be relevant in the pathogenesis of prostatic disease.
Assuntos
Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/toxicidade , Próstata/efeitos dos fármacos , Próstata/patologia , Administração Oral , Animais , Relação Dose-Resposta a Droga , Imuno-Histoquímica , Masculino , Tamanho do Órgão/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
Populations in the Amazon are exposed to organic mercury via consumption of contaminated foods. These ethnic groups consume a specific plant seed "annatto" which contains certain carotenoids. The aim of this study was to find out if these compounds (bixin, BIX and norbixin, NOR), protect against DNA-damage caused by the metal. Therefore, rats were treated orally with methylmercury (MeHg) and with the carotenoids under conditions that are relevant to humans. The animals were treated either with MeHg (30 µg/kg/bw/day), BIX (0.1-10 mg/kg/bw/day), NOR (0.01-1.0 mg/kg/bw/day) or combinations of the metal compound and the carotenoids consecutively for 45 days. Subsequently, the glutathione levels (GSH) and the activity of catalase were determined, and DNA-damage was measured in hepatocytes and leukocytes using single cell gel electrophoresis assays. Treatment with the metal alone caused a decrease in the GSH levels (35%) and induced DNA damage, which resulted in increased DNA migration after electrophoresis in liver and blood cells, whereas no effects were seen with the carotenoids alone. When BIX or NOR were given in combination with organic mercury, the intermediate and the highest concentrations of the carotenoids (1.0 and 10.0 mg/kg/bw/day BIX and 0.1 and 1.0 mg/kg/bw/day NOR) protected against DNA-damage. Furthermore, we found with both carotenoids, a moderate increase in the GSH levels in both metal-treated and untreated animals, while the activities of catalase remained unchanged. Our results indicate that consumption of BIX and NOR may protect humans against the adverse health effects caused by exposure to organic mercury.
Assuntos
Bixaceae/química , Carotenoides/química , Carotenoides/farmacologia , Dano ao DNA/efeitos dos fármacos , Poluentes Ambientais/toxicidade , Compostos de Metilmercúrio/toxicidade , Extratos Vegetais/química , Análise de Variância , Animais , Carotenoides/administração & dosagem , Catalase/metabolismo , Ensaio Cometa , Glutationa/metabolismo , Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/sangue , Estrutura Molecular , Oxirredução/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
OBJECTIVE: To assess the dependence on fish consumption of families and its impact on nutritional status and neurodevelopment of pre-school children. DESIGN: Cross-sectional study that measured children's hair mercury (HHg) as an indicator of family fish consumption, growth (anthropometric Z-scores, WHO standards) and neurological (Gesell developmental scores (GDS)) development. SETTING: Traditional living conditions among families residing in the area adjacent to the Samuel Dam (Western Amazon) hydroelectric reservoir. SUBJECTS: Two hundred and forty-nine pre-school children (1-59 months of age) from families transitioning from the traditional Amazonian lifestyle. RESULTS: Family fish consumption was significantly correlated with children's HHg concentration (Spearman's r=0.246, P<0.0001); however, HHg had no significant association with growth (Z-scores). Overall, the prevalence of severe malnutrition, i.e. stunting (height-for-age Z-score (HAZ)≤-3), underweight (weight-for-age Z-score (WAZ)≤-3) and wasting (weight-for-height Z-score (WHZ)≤-3) was 5.2% (n 13), 0% and 0.8% (n 2), respectively. The prevalence of moderate stunting (HAZ≥-3 to ≤-2), underweight (WAZ≥-3 to ≤-2) and wasting (WHZ≥-3 to ≤-2) was 8.8% (n 22), 2.4% (n 6) and 4.8% (n 12), respectively. Although 76% of the children showed adequate GDS (>85), multiple regression analysis showed that fish consumption (as HHg) had no impact on GDS, but that some variables did interact significantly with specific domains (motor and language development). CONCLUSIONS: The study showed that the families' shift in fish consumption had no negative impact on the growth of young children and that ensuing methylmercury exposure has not been a noticeable neurodevelopmental hindrance.
Assuntos
Desenvolvimento Infantil/efeitos dos fármacos , Transtornos da Nutrição Infantil/epidemiologia , Estilo de Vida , Compostos de Metilmercúrio , Alimentos Marinhos/análise , Animais , Brasil , Desenvolvimento Infantil/fisiologia , Pré-Escolar , Estudos Transversais , Exposição Ambiental/efeitos adversos , Exposição Ambiental/análise , Feminino , Peixes/metabolismo , Contaminação de Alimentos , Cabelo/química , Humanos , Lactente , Masculino , Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/efeitos adversos , Compostos de Metilmercúrio/análise , Estado Nutricional , Centrais Elétricas , Alimentos Marinhos/estatística & dados numéricosRESUMO
Methylmercury (MeHg) can cause deleterious effects in vertebrate tissues, particularly in the central nervous system. MeHg interacts with sulfhydryl groups from low and high molecular weight thiols in the blood, which can facilitate MeHg uptake into different tissues. The purpose of this study was to examine the effect of MeHg-Cysteine (MeHg-Cys) complex administration on Hg-uptake in cerebral areas (cortex and cerebellum), liver and kidney of adult mice. Animals were divided into four groups: control (1 mL/kg distilled water), MeHg (2 mg/kg), Cys (2 mg/kg) and MeHg-Cys complex (0.8 molar ratio). Mice received one intraperitoneal injection per day for 60 consecutive days. Treatment with MeHg significantly increased mercury concentrations in all tissues analysed when compared with the control group. The accumulation of mercury in brain and in liver was further increased in animals that received MeHg-Cys complex when compared with the MeHg alone group. However, renal Hg decreased in MeHg-Cys treated mice, when compared with the group treated only with MeHg. In summary, the transport of MeHg-Cys complex was tissue-specific, and we observed an increase in its uptake by liver and brain as well as a decrease in kidney.
Assuntos
Cisteína/análogos & derivados , Rim/metabolismo , Fígado/metabolismo , Compostos de Metilmercúrio/metabolismo , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Cisteína/administração & dosagem , Cisteína/metabolismo , Injeções Intraperitoneais , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Masculino , Compostos de Metilmercúrio/administração & dosagem , Camundongos , Distribuição TecidualAssuntos
Comportamento Alimentar , Peixes , Contaminação de Alimentos/análise , Cabelo/química , Mercúrio/análise , Adulto , Animais , Criança , Feminino , Humanos , Masculino , Mercúrio/toxicidade , Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/efeitos adversos , Gravidez , Medição de RiscoRESUMO
Pregnant rats were exposed to ethanol (EtOH) and/or methyl mercury (MeHg) during fetal brain development. Nitrergic activity was quantified by densitometric measurement of formazan deposits in the hippocampus, cerebellum and striatum of two-month-old offspring following histochemical assay for NADPH-diaphorase (NADPH-d) activity. Compared to control subjects, an increase in nitrergic activity was found in the molecular layer of dentate gyrus and in the lacunosum molecular and stratum radiatum of CA1 (cornus amoni 1) in the EtOH+MeHg group, whereas a single administration of EtOH increased the activity in all striatal segments. The cerebellum seems to be less sensitive at this time-point to intoxication, and presented an increase only at the molecular layer of EtOH-exposed animals when compared to the MeHg and EtOH+MeHg groups (ANOVA, one-way followed by Tukey's test, p<0.05 or p<0.01). Taken together, results suggest that developmental exposure to EtOH and MeHg, singularly or in combination, alters nitrergic activity in adult rat in different ways depending on the region and layer of the central nervous system (CNS), and that these alterations might be related to different local metabolic properties.
Assuntos
Encéfalo/efeitos dos fármacos , Etanol/administração & dosagem , Etanol/toxicidade , Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/toxicidade , NADPH Desidrogenase/metabolismo , Óxido Nítrico/metabolismo , Efeitos Tardios da Exposição Pré-Natal , Animais , Encéfalo/metabolismo , Feminino , Imuno-Histoquímica , Masculino , Gravidez , Ratos , Ratos WistarRESUMO
In this study, we examined the effects of low levels and sub-chronic exposure to methylmercury (MeHg) on butyrylcholinesterase (BuChE) activity in rats. Moreover, we examined the relationship between BuChE activity and oxidative stress biomarkers [delta-aminolevulinic acid dehydratase (delta-ALA-D) and malondialdehyde levels (MDA)] in the same animals. Rats were separated into three groups (eight animals per group): (Group I) received water by gavage; (Group II) received MeHg (30 microg/kg/day) by gavage; (Group III) received MeHg (100 microg/kg/day). The time of exposure was 90 days. BuChE and ALA-D activities were measured in serum and blood, respectively; whereas MDA levels were measured in plasma. We found BuChE and ALA-D activities decreased in groups II and III compared to the control group. Moreover, we found an interesting negative correlation between plasmatic BuChE activity and MDA (r = -0.85; p < 0.01) and a positive correlation between plasmatic BuChE activity and ALA-D activities (r = 0.78; p < 0.01), thus suggesting a possible relationship between oxidative damage promoted by MeHg exposure and the decrease of BuChE activity. In conclusion, long-term exposure to low doses of MeHg decreases plasmatic BuChE activity. Moreover, the decrease in the enzyme is strongly correlated with the oxidative stress promoted by the metal exposure. This preliminary finding highlights a possible mechanism for MeHg to reduce BuChE activity in plasma. Additionally, this enzyme could be an auxiliary biomarker on the evaluation of MeHg exposure.
Assuntos
Butirilcolinesterase/efeitos dos fármacos , Compostos de Metilmercúrio/toxicidade , Animais , Biomarcadores/metabolismo , Butirilcolinesterase/sangue , Butirilcolinesterase/metabolismo , Relação Dose-Resposta a Droga , Masculino , Malondialdeído/metabolismo , Compostos de Metilmercúrio/administração & dosagem , Estresse Oxidativo/efeitos dos fármacos , Sintase do Porfobilinogênio/efeitos dos fármacos , Sintase do Porfobilinogênio/metabolismo , Ratos , Ratos Wistar , Fatores de TempoRESUMO
Studies involving alcohol and its interactions with other neurotoxicants represent the focus of several works of research due to the fact that the use of alcohol can sometimes leads to serious health problems. Fetal exposure to alcohol and mercury has a high incidence in some regions of Brazil, where there are pregnant women who are alcoholics and live in mining areas. This work was conducted to examine the effects of combined exposure to ethanol (EtOH) and methylmercury (MeHg) in rats during the development of the central nervous system (CNS). Experimental behavioral animal models/tests were used in order to examine locomotion, anxiety, depression and memory. Pregnant rats received tap water or EtOH 22.5% w/v (6.5 g/kg per day), by gavage) during pregnancy and breast-feeding. On the 15th day of pregnancy, some groups received 8 mg/kg of MeHg (by gavage). The groups were as follows: control, EtOH, MeHg and EtOH+MeHg. The experimental results showed that the EtOH, MeHg and EtOH+MeHg groups reduced the percentage of frequency and time spent in the open arms entries of the elevated plus-maze (EPM) test, when compared to the control group. This result suggests an anxiogenic behavioral response. The MeHg group increased locomotor activity in the arena and the immobility time in the forced swimming test, suggestive of depression-like behavior. The EtOH+MeHg group showed greater reductions in the percentages of frequency and time spent in the open arms entries in the EPM test, suggesting a sedative-behavior since the frequency of enclosed arm entries was affected. In the inhibitory avoidance task, the EtOH+MeHg group reduced the latency of the step-down response onto the grid floor, suggesting a cognitive and behavior dysfunctions. Taken together, the results suggest that EtOH and/or MeHg intoxication during the developing CNS may be a risk for deficits related to locomotor impairment, anxiety, depression and neurocognitive functions. There is a possibility that EtOH may prevent some of the MeHg responses, but the precise mechanism of action involved in this process needs to be considered for future research.
Assuntos
Sistema Nervoso Central/efeitos dos fármacos , Sistema Nervoso Central/embriologia , Etanol/toxicidade , Troca Materno-Fetal , Compostos de Metilmercúrio/toxicidade , Prenhez , Animais , Aprendizagem da Esquiva/efeitos dos fármacos , Interações Medicamentosas , Etanol/administração & dosagem , Feminino , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/análise , Atividade Motora/efeitos dos fármacos , Gravidez , Ratos , NataçãoRESUMO
The aim of the present report is to investigate in detail morphometric changes of axon terminals of area 17 of adult cat induced by methylmercury intoxication. Six adult male cats (Felix catus), with 12 h day-light cycle and ad libitum water and food regimen, received a single dose of MeHgCl (6.4 mg/kg) dissolved in milk, whereas control subjects (n=6) received only milk. After 30 days, biocytin iontophoretic injections were done into the area 17, (Horsley-Clark coordinates between AP 3.0-6.0) on the crown of the lateral gyrus, near the border with area 18. MeHg and inorganic Hg (Hgi) concentrations were measured in the brain parenchyma of intoxicated cats and corresponded on average to 1.39+/-0.3 and 6.79+/-0.6 ppm (mean+/-s.e.m.) respectively. Twenty four hours after iontophoresis, aldehyde fixed brain sections (200 microm thick), were processed to reveal biocytin labeled terminals. Axonal microscopic 3D reconstructions using Neurolucida software (Microbright Systems Inc.) allowed estimations of boutons, branching points and segment densities for each terminal. Cluster analysis of morphometric axonal features from control and intoxicated group revealed, two distinct axon families (Type I and II) as described elsewhere. Total density values of boutons, branching points and segment densities of intoxicated group, decreased 81, 59 and 91% respectively, as compared to the control group (ANOVA two-way, Bonferroni a priori test p<0.05). Altered axonal morphology associated with MeHg, appeared early in the disease (30 days after contamination), revealing new aspects of the neuronal pathology of the methylmercury intoxication in the visual cortex.
Assuntos
Encéfalo/efeitos dos fármacos , Compostos de Metilmercúrio/toxicidade , Terminações Pré-Sinápticas/efeitos dos fármacos , Córtex Visual/efeitos dos fármacos , Administração Oral , Análise de Variância , Animais , Encéfalo/patologia , Gatos , Imageamento Tridimensional/métodos , Masculino , Compostos de Metilmercúrio/administração & dosagem , Microscopia Confocal , Terminações Pré-Sinápticas/patologia , Fatores de Tempo , Córtex Visual/patologiaRESUMO
BACKGROUND: In 2005, 84% of Wayana Amerindians living in the upper marshes of the Maroni River in French Guiana presented a hair mercury concentration exceeding the limit set up by the World Health Organization (10 microg/g). To determine whether this mercurial contamination was harmful, mice have been fed diets prepared by incorporation of mercury-polluted fish from French Guiana. METHODS: Four diets containing 0, 0.1, 1, and 7.5% fish flesh, representing 0, 5, 62, and 520 ng methylmercury per g, respectively, were given to four groups of mice for a month. The lowest fish regimen led to a mercurial contamination pressure of 1 ng mercury per day per g of body weight, which is precisely that affecting the Wayana Amerindians. RESULTS: The expression of several genes was modified with mercury intoxication in liver, kidneys, and hippocampus, even at the lowest tested fish regimen. A net genetic response could be observed for mercury concentrations accumulated within tissues as weak as 0.15 ppm in the liver, 1.4 ppm in the kidneys, and 0.4 ppm in the hippocampus. This last value is in the range of the mercury concentrations found in the brains of chronically exposed patients in the Minamata region or in brains from heavy fish consumers. Mitochondrial respiratory rates showed a 35-40% decrease in respiration for the three contaminated mice groups. In the muscles of mice fed the lightest fish-containing diet, cytochrome c oxidase activity was decreased to 45% of that of the control muscles. When mice behavior was assessed in a cross maze, those fed the lowest and mid-level fish-containing diets developed higher anxiety state behaviors compared to mice fed with control diet. CONCLUSION: We conclude that a vegetarian diet containing as little as 0.1% of mercury-contaminated fish is able to trigger in mice, after only one month of exposure, disorders presenting all the hallmarks of mercurial contamination.
Assuntos
Modelos Animais de Doenças , Peixes , Contaminação de Alimentos , Intoxicação por Mercúrio/etiologia , Compostos de Metilmercúrio/intoxicação , Compostos de Metilmercúrio/toxicidade , Adulto , Animais , Ansiedade/induzido quimicamente , Feminino , Guiana Francesa , Expressão Gênica , Humanos , Indígenas Sul-Americanos , Masculino , Intoxicação por Mercúrio/genética , Intoxicação por Mercúrio/metabolismo , Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/farmacocinética , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Mitocôndrias Musculares/efeitos dos fármacos , Mitocôndrias Musculares/metabolismo , Mutação , Consumo de Oxigênio/efeitos dos fármacosRESUMO
Methylmercury is a potent toxic present in Amazonian fish species due to gold mining activities. In the present work, we investigated the morphological effects of methylmercury in liver and kidney of Hoplias malabaricus feeding contaminated prey fish over 70 days. Two groups of nine mature fish (tested and control) were acclimatized for four weeks to laboratory conditions and then the tested group fed prey fish previously contaminated at an additional level of 0.075 microg MeHg g(-1) at 5-day intervals and over 14 successive intervals whereas control group fed uncontaminated fish. H. malabaricus specimens were then dissected for chemical and morphological analyses. The low and realistic level of MeHg in the prey fish induced a low increase of total mercury in liver (1.8-fold) and muscle (2.2-fold). The biomagnification factor (Hg in predator/Hg in prey) reached 142 in liver and 21 in muscle and was indicative of a relatively fast contamination of internal organs by dietary exposure. The liver of exposed individuals presented leukocyte infiltration, increased number of melano-macrophage centers, necrotic areas and lesions in Disse's space. Evident disorder and chaos in cytoskeleton organization suggest a strong toxic effect in hepatocytes, such as organelles positioning and movement, vesicles traffic and secretion. Head kidney showed large necrosis areas, increased number of melano-macrophages centers, phagocytic areas, intercellular space among parenquimal cells and atypical cells. Injuries and damages to tissues suggest too slow defense mechanisms to immobilize or eliminate ingested methylmercury, demonstrating that the sensitivity of fish cells to methylmercury exposure is higher than it has been previously described in the literature.
Assuntos
Peixes , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Compostos de Metilmercúrio/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Brasil , Dieta , Cadeia Alimentar , Contaminação de Alimentos , Rim/metabolismo , Rim/patologia , Fígado/metabolismo , Fígado/patologia , Compostos de Metilmercúrio/administração & dosagem , Compostos de Metilmercúrio/metabolismo , Fatores de Tempo , Clima Tropical , Poluentes Químicos da Água/administração & dosagem , Poluentes Químicos da Água/metabolismoRESUMO
Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration.
Assuntos
Peixes , Compostos de Metilmercúrio/toxicidade , Células Horizontais da Retina/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Eletrofisiologia , Compostos de Metilmercúrio/administração & dosagem , Células Horizontais da Retina/fisiologiaRESUMO
Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration.