RESUMO
BACKGROUND: Prevention and early diagnosis have the greatest potential for public health and are the most effective method in the long-term to control oral cancer. The aim was to apply PAP staining together with AgNOR staining and morphometric analysis in oral exfoliative cytology, to determine the sensitivity and specificity of these methods in the detection of malignant changes for the purposes of both initial population monitoring and follow-up. METHODS: AgNOR, Papanicolau, and morphometric tests were conducted in samples of patients with oral cancer, oral potentially malignant disorders and controls (opposite side of lesions). Specificity and sensitivity values for each stain method and the curve under ROC area were estimated. RESULTS: The diagnostic variables which allowed greatest accuracy in identifying malignancy relative to the healthy control were cluster (76.92%), satellite (75.64%), and total (90%). The diagnosis was seen to be associated with PAP and total AgNOR, total AgNOR and PAP, total AgNOR and satellites and clusters, and total AgNOR nuclear area/cytoplasmic area ratio. CONCLUSIONS: The total number of AgNOR is a reliable marker for detecting neoplastic cells; this method increases sensitivity and specificity by decreasing the likelihood of false negatives or positives, as the accuracy obtained was 90%. It is also a low-cost, non-invasive, simple methodology that can be recommended to help the early detection of oral cancer and monitoring of patients with a first diagnosis of cancer.
Assuntos
Corantes , Citodiagnóstico/métodos , Detecção Precoce de Câncer/métodos , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos Nucleares/ultraestrutura , Área Sob a Curva , Núcleo Celular/ultraestrutura , Estudos Transversais , Citodiagnóstico/estatística & dados numéricos , Citoplasma/ultraestrutura , Detecção Precoce de Câncer/estatística & dados numéricos , Feminino , Humanos , Leucoplasia Oral/diagnóstico , Leucoplasia Oral/patologia , Líquen Plano Bucal/diagnóstico , Líquen Plano Bucal/patologia , Masculino , Pessoa de Meia-Idade , Região Organizadora do Nucléolo/ultraestrutura , Teste de Papanicolaou/estatística & dados numéricos , Curva ROC , Sensibilidade e Especificidade , Coloração pela Prata/estatística & dados numéricos , Adulto JovemRESUMO
This study was performed on oral squamous cell carcinomas (OSCC) in order to investigate the relation between the number of interphase silver-stained nucleolar organizer regions (AgNORs) and the immunolabeling of proliferation-associated markers, using antibodies to Ki-67 and proliferating cell nuclear antigen (PCNA). Fifteen consecutive cases of oral squamous cell carcinoma were used and a double staining technique was performed in order to quantify the number of NORs in PCNA-positive and -negative cells as well as in Ki-67-positive and -negative cells. Our results showed a higher mean number of AgNORs in PCNA- and Ki-67-positive cells than in PCNA- and Ki-67-negative cells. We concluded that there is an association between cell proliferation and AgNOR score in OSCC.
Assuntos
Carcinoma de Células Escamosas/metabolismo , Antígeno Ki-67/metabolismo , Neoplasias Bucais/metabolismo , Região Organizadora do Nucléolo/metabolismo , Antígeno Nuclear de Célula em Proliferação/metabolismo , Coloração pela Prata/métodos , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Soalho Bucal , Mucosa Bucal/metabolismo , Coloração pela Prata/estatística & dados numéricosRESUMO
Se describe una técnica sencilla y altamente sensible para la identificación inmunológica de proteínas en orina sin concentración previa, basada en la alta sensibilidad de las tinciones con metales pesados, como plata y oro, al estado coloidal (100 veces más sensible que el azul brillante de Coomassie (CBB) R y G 250). Posteriormente a la corrida electroforética convencional se efectuó una inmunofijación utilizando una cantidad de antisuero monoespecífico en el orden de 3,5 Al/cm² de gel. El contacto antígeno-antisuero se mantuvo durante 30 minutos y posteriormente se efectuaron lavados con sucesivos recambios de solución fisiológica y se aplicaron las coloraciones argénticas, áurica y con CBB R 250. Bajo las condiciones de trabajo utilizadas se lograron identificar satisfactoriamente las cadenas livianas k y ? monoclonales en proteinurias de Bence Jones (BJ) positivo, y la ß2 microglobulina en proteinurias de tipo tubular, con un límite de sensibilidad del orden de los 5 x 10-4ug de proteína/mm2 de gel. Esta técnica resultó de gran utilidad en el estudio del cuadro uroproteico y se presenta como un método simple, rápido, de alta sensibilidad y baja relación costo/beneficio, para el estudio de los distintos cuadros de proteinuria en muestras sin concentrar (AU)