RESUMO
AIM: To develop an endodontic sealer with amoxicillin-loaded microsphere and to evaluate its properties. METHODS: Experimental sealer was obtained by mixing 70wt% UDMA, 15wt% GDMA and 15wt% BISEMA. In addition, CQ, DHEPT and PB were incorporated at 1 mol% and 0.01wt% BHT and 10wt% YbF3. Microspheres were produced by drying in spray dryer. Two experimental groups (10 and 15wt% of microspheres) and one control group (without microspheres) were formulated. The sealers were evaluated for the degree of conversion (n=3), degradation in solvent (n=3), drug release profile (n=3), antimicrobial activity (n=3), flow (n=3), film thickness (n=3) and cytotoxicity (n=3). Data were analyzed by ANOVA and Tukey with significance level of 5%. RESULTS: Microespheres presented a mean size of 2.664µm. Immediate degree of conversion ranged from 51.73% to 55.13% and the 24h degree of conversion ranged from 60.79% to 73.80%. Upon solvent degradation 54.44% and 56.21% reduction in hardness were observed for 10% and 15% concentrations respectivelly. The drug release profile showed an average release of 73.76% of the drug in 96h. Significant reduction in antimicrobial activity was observed for 10% concentration after 24h, 48h and 96h compared to control. The flow and film thickness showed values in accordance to the ISO 6876. Cytotoxicity showed high cellular viability. CONCLUSION: The addition of up to 10% of microspheres containing amoxicillin presented antimicrobial activity and did not alter the properties of the experimental endodontic cement. CLINICAL SIGNIFICANCE: Amoxicillin microspheres with its antimicrobial activity in root canal sealers could reduce reintervations in endodontics when persistent bacteria or reinfection takes place in root canal system.
Assuntos
Amoxicilina/administração & dosagem , Amoxicilina/farmacologia , Resinas Epóxi/química , Resinas Epóxi/farmacologia , Microesferas , Materiais Restauradores do Canal Radicular/química , Materiais Restauradores do Canal Radicular/farmacologia , Análise de Variância , Anti-Infecciosos/farmacologia , Hidroxitolueno Butilado , Sobrevivência Celular , Contagem de Colônia Microbiana , Relação Dose-Resposta a Droga , Liberação Controlada de Fármacos , Fibroblastos/efeitos dos fármacos , Fluoretos , Dureza , Humanos , Teste de Materiais , Metacrilatos , Solventes , Fatores de Tempo , Itérbio , Cimento de Óxido de Zinco e Eugenol/administração & dosagem , Cimento de Óxido de Zinco e Eugenol/química , Cimento de Óxido de Zinco e Eugenol/farmacologiaRESUMO
El objetivo de este trabajo fue evaluar clínica y radiográficamente la efectividad de la pasta antibiótica CTZ en pulpotomías de molares primarios. Se realizó un ensayo clínico aleatorio controlado, en 40 molares primarios de 40 preescolares con edades entre 3 y 6 años. Los pacientes fueron seleccionados y asignados aleatoriamente a dos grupos: Formocresol (n=20), CTZ (Cloranfenicol-Tetraciclina-Oxido de Zinc Eugenol, n=20). Los dientes fueron restaurados con ionómero de vidrio y coronas metálicas de acero preformadas. La evaluación clínica y radiográfica fue realizada a los 3 y 6 meses. Se utilizó el programa SPSS v.17 para el análisis de los datos, y la aplicación del Test Exacto de Fisher al 5 %. El comportamiento clínico a los 3 meses mostró 75 % de éxito para los molares tratados con formocresol y 70 % para el CTZ. Radiográficamente se obtuvo 90 % de éxito para el grupo con formocresol y 100 % para la pasta CTZ. A los 6 meses el éxito clínico del formocresol fue de 85 % y del CTZ 80 %. El éxito radiográfico mostrado fue 65 % para los atendidos con formocresol y 80 % para el CTZ (p > 0,05). No se observaron diferencias significativas entre los grupos de tratamiento. La pasta CTZ es una alternativa en el tratamiento de pulpotomías de molares temporales. Ofrece un efecto antimicrobiano, estabilización del proceso de reabsorción radicular, sin ocasionar daños a la formación del diente permanente.
The objective of this study to evaluate the clinical and radiographic effectiveness of antibiotic paste CTZ in pulpotomy of primary molars. A randomized controlled clinical trial was performed, in 40 molars of 40 patients aged 3 to 6 years. Were selected and randomly assigned to two groups: Formocresol (n=20), CTZ (Chloramphenicol-Tetracycline-Zinc Oxide Eugenol, n=20). The teeth were restored with glass ionomer and performed steel metal crowns. Clinical and radiographic evaluation procedure was performed at 3 and 6 months. SPSS v.17 program for data analysis, and application of the Fisher exact test was used 5 %. The clinical behavior at 3 months showed 75 % success rate for molars treated with formocresol and 70 % for the CTZ. Radiographically 90 % success rate for the group with formocresol and 100 % for the CTZ paste was obtained. At 6 months formocresol clinical success was 85 % and 80 % CTZ. Radiographic success was shown 65 % for those treated with formocresol and 80 % for the CTZ (p>0.05). No significant differences in the results shown were observed. CTZ paste is an alternative in the treatment of pulpotomy of molars. It provides an antimicrobial effect, stabilizing the process of root resorption, without causing damage to the permanent tooth formation.
Assuntos
Humanos , Masculino , Feminino , Cloranfenicol/administração & dosagem , Pulpotomia/métodos , Tetraciclina/administração & dosagem , Cimento de Óxido de Zinco e Eugenol/administração & dosagem , Formocresóis/administração & dosagem , Dente Molar/diagnóstico por imagem , Radiografia Dentária , Dente Decíduo/diagnóstico por imagemRESUMO
INTRODUCTION: An evaluation was made of the connective tissue reaction in rats after subcutaneous implantation of methacrylate resin-based sealers (EndoREZ [Ultradent Products, Inc, South Jordan, UT] with a polymerization accelerator and RealSeal [Sybron Dental Specialties, Orange, CA]) and Pulp Canal Sealer (Sybron Dental Specialties), a zinc oxide and eugenol-based sealer used as the control. METHODS: Silicone tubes containing the test materials were implanted in 24 Wistar rats. Solid silicone rods of the same size served as the negative controls. After 10, 30, and 90 days, the animals (n = 8 per period) were euthanized and the implants with surrounding tissues dissected and processed for routine histological evaluation. A four-category evaluation system was used to measure and record the microscopic observations according to the thickness of a fibrous capsule, the vascular changes, and the various types of inflammatory cells. RESULTS: Initially, a severe inflammatory reaction was observed of the soft tissues in direct contact with both EndoREZ/Accelerator and Real Seal. The severity decreased over time and was resolved at the end of the experiment. Pulp Canal Sealer showed a severe tissue reaction for all observation periods. The negative controls showed an initial mild to moderate inflammatory reaction. After 30 days, healthy fibrous connective tissue was observed, which increased over time. After 10 days, no statistically significant differences between the experimental groups were observed. After 90 days, EndoREZ and RealSeal were statistically significantly less toxic than Pulp Canal Sealer (p > 0.05). CONCLUSIONS: After 90 days, both methacrylate resin-based sealers were considered biologically acceptable when implanted in subcutaneous connective tissues of the rat. Pulp Canal Sealer remained toxic for the duration of the study.
Assuntos
Resinas Compostas/toxicidade , Tecido Conjuntivo/efeitos dos fármacos , Reação a Corpo Estranho/induzido quimicamente , Implantes Experimentais , Materiais Restauradores do Canal Radicular/toxicidade , Cimento de Óxido de Zinco e Eugenol/toxicidade , Animais , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/toxicidade , Resinas Compostas/administração & dosagem , Seguimentos , Inflamação/induzido quimicamente , Masculino , Ratos , Ratos Wistar , Tela Subcutânea/efeitos dos fármacos , Testes de Toxicidade/métodos , Cimento de Óxido de Zinco e Eugenol/administração & dosagemRESUMO
The objective of this research was to evaluate, in vitro, the importance of the correct manipulation of endodontic sealers, correlating it with flow rate and with the consequent obturation of root canals. Twenty-four human canines were prepared, 1 mm from the apex, with K-files up to size 50, by means of the step-back technique. Six lateral canals were then drilled in each tooth, with size 10 file fixed to a low-speed handpiece. The teeth were randomly divided into 4 groups, and root canals were obturated either with the Endométhasoneregister mark or target sealer or Grossman sealer, prepared at ideal or incorrect clinical consistency. After obturation by means of the lateral condensation technique, the teeth were radiographed and evaluated as to the number of sealed lateral canals. Statistical analysis revealed significant differences (p < 0.001) between the tested sealers, and indicated the higher capacity of the well-manipulated Grossman sealer to fill lateral canals. It can be concluded that the flow rate of a sealer and its correct manipulation are very important for the satisfactory obturation of lateral canals.
Assuntos
Administração Tópica , Anti-Inflamatórios/administração & dosagem , Dexametasona/administração & dosagem , Formaldeído/administração & dosagem , Hidrocortisona , Materiais Restauradores do Canal Radicular , Obturação do Canal Radicular/métodos , Timol/análogos & derivados , Timol/administração & dosagem , Cimento de Óxido de Zinco e Eugenol/administração & dosagem , Dente Canino , Combinação de Medicamentos , Humanos , Estatísticas não ParamétricasRESUMO
El propósito de este estudio in vitro fue evaluar el efecto de los cementos temporales con eugenol (CTE) y sin eugenol (CTS) en la microfiltración de las resinas compuestas usando 2 sistemas adhesivos monocomponentes diferentes. Se utilizaron 48 premolares en las que se realizaron cavidades clase V. Las piezas se dividieron al azar en 2 grupos: 1) El grupo A, fue obturado en un primer momento con el CTE y el grupo B fue obturado en un primer momento con un CTS, para proceder a su retiro luego de 7 días; cada grupo se dividió en 2 subgrupos de 12 piezas cada uno restaurándose con dos diferentes sistemas adhesivos y sus respectivas resinas compuestas. Luego se sometieron a un Ciclaje Térmico por 500 veces. Para evaluar la microfiltración se sumergieron en azul de metileno baferado, y se observó por medio de un microscopio estereoscópico a 40X. En todos los subgrupos se encontró mejor comportamiento en oclusal, comparado con gingival al usar CTE y CTS previo a la colocación del sistema adhesivo y de la resina compuesta. No se encontraron diferencias estadísticamente significativas.
Assuntos
Cimento de Óxido de Zinco e Eugenol/administração & dosagem , Eugenol/administração & dosagem , MicropeneiramentoRESUMO
Avaliou-se histopatologicamente a resposta pulpar de dentes de cães submetidos a pulpotomia e tratamento do remanescente pulpar com misturas de paramonoclorofenol canforado (3,5 : 6,5) e (2,5 : 7,5) mais furacin e ou óxido de zinco e eugneol em períodos de 7, 45 e 90 dias. Concluiu-se que as misturas de paramonoclorofenol canfordo (3,5 : 6,5) e (2,5 : 7,5) mais furacin mostraram-se irritantes ao tecido conjuntivo pulpar, levando à morte pulpar em 86,75 por cento e 60,98 por cento, respectivamente. O óxido de zinco e eugenol mostrou melhor comportamento, observando-se vitalidade pulpar (89,42 por cento), irritação persistente e decrescente no decorrer dos períodos. Em todos os casos observou-se manutenção parcial ou total das vitalidades nas extensões conjuntivas do delta apical menos expressiva no Grupo PMCC (3,5 : 6,5) - furacin