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Int J Biol Macromol ; 187: 325-331, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34280448

RESUMO

Understanding nonionic surfactant-protein interactions is fundamental from both technological and scientific points of view. However, there is a complete absence of kinetic data for such interactions. We employed surface plasmon resonance (SPR) to determine the kinetic and thermodynamic parameters of bovine lactoferrin-Brij58 interactions at various temperatures under physiological conditions (pH 7.4). The adsorption process was accelerated with increasing temperature, while the desorption rate decreased, resulting in a more thermodynamically stable complex. The kinetic energetic parameters obtained for the formation of the activated complex, [bLF-Brij58]‡, indicated that the potential energy barrier for [bLF-Brij58]‡ formation arises primarily from the reduction in system entropy. [bLF-Brij58]○ formation was entropically driven, indicating that hydrophobic interactions play a fundamental role in bLF interactions with Brij58.


Assuntos
Cetomacrogol/metabolismo , Lactoferrina/metabolismo , Tensoativos/metabolismo , Temperatura , Adsorção , Cetomacrogol/química , Entropia , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Cinética , Lactoferrina/química , Ligação Proteica , Ressonância de Plasmônio de Superfície , Tensoativos/química
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