RESUMO

Different anesthetic agents are commonly used during procedures performed in aquaculture to minimize stress and maximize fish welfare during handling. A Kinguio fish was treated with a history of a mass near the left eye. In the clinical evaluation, a pedunculated neoformation was found in the periocular region. The fish was submitted to anesthesia for surgical removal of the mass, which was later referred to histopathological evaluation, revealing myxoma. The anesthesia protocol used 1% propofol at a concentration of 5 mg/liter of water and morphine at a dose of 5 mg/kg, intramuscularly. Immediately after the end of the procedure, the fish was returned to the aquarium and its anesthetic recovery was observed. The objective of this report is to describe a surgical procedure in kinguio fish and to determine the efficacy of propofol associated with morphine in this species. As a result, complete immobilization of the fish was obtained with propofol, reaching the fourth stage of anesthesia and the administration of morphine suggested being able to provide effective and long-lasting analgesia. It is concluded that despite the positive results obtained, anesthesia in fish still needs to be extensively investigated to refine analgesia protocols during procedures that cause pain and stress.

Diferentes agentes anestésicos são usados durante procedimentos realizados na aquicultura para minimizar o estresse e maximizar o bem-estar dos peixes durante o processo de manejo. Um peixe kinguiu foi atendido com histórico de presença de uma massa próxima ao olho esquerdo. Na avaliação clínica, constatou-se uma neoformação pedunculada na região periocular. O peixe foi submetido à anestesia para remoção cirúrgica da massa, que posteriormente foi encaminhada para avaliação histopatológica, a qual revelou mixoma. Como protocolo de anestesia, utilizou-se propofol 1% diluído em água, na concentração de 5mg/litro, e morfina, na dose de 5mg/kg, na concentração de 10mg/mL, intramuscular. Imediatamente após o procedimento, o peixe foi devolvido ao aquário para observação da recuperação anestésica. O objetivo deste relato é descrever um procedimento cirúrgico em peixe kinguio e determinar a eficácia do propofol associado à morfina nessa espécie. Como resultados, obteve-se a imobilização completa do peixe com o uso do propofol, atingindo o quarto estágio da anestesia, e a administração da morfina sugeriu ser capaz de proporcionar analgesia eficaz e duradoura. Conclui-se que, apesar dos resultados positivos obtidos, a anestesia em peixes precisa ainda ser bastante investigada para refinar os protocolos de analgesia durante os procedimentos que causam dor e estresse.

Assuntos

RESUMO

This work investigated the effect of the H1 receptor blockade in the forebrain of ablated Carassius auratus in a simple stimulus-response learning task using a T-maze test with positive reinforcement. The goldfish were submitted to surgery for removal of both telencephalic lobes five days before beginning the experiment. A T-shaped glass aquarium was employed, with two feeders located at the extremities of the long arm. One of the two feeders was blocked. The experimental trials were performed in nine consecutive days. Each fish was individually placed in the short arm and confined there for thirty seconds, then it was allowed to swim through the aquarium to search for food for ten minutes (maximum period). Time to find food was analysed in seconds. Animals were injected intraperitoneally with chlorpheniramine (CPA) at 16 mg/kg of body weight or saline after every trial, ten minutes after being placed back in the home aquarium. The results show that all the training latencies of the A-SAL group were higher than the latencies of the S-SAL group. The S-SAL group had decreased latencies from the second trial on, while the S-CPA group showed decreased latencies after the fourth trial. The A-SAL group showed reduced latencies after the fifth trial, but the A-CPA group maintained the latencies throughout the experiment. This suggests that CPA impairs the consolidation of learning both on telencephalon ablated animals and in sham-operated ones through its action on mesencephalic structures of the brain and/or on the cerebellum in teleost fish.

Assuntos

RESUMO

This work investigated the effect of the Hj receptor blockade in the forebrain of ablated Carassius auratus in a simple stimulus-response learning task using a T-maze test with positive reinforcement. The goldfish were submitted to surgery for removal of both telencephalic lobes five days before beginning the experiment. A T-shaped glass aquarium was employed, with two feeders located at the extremities of the long arm. One of the two feeders was blocked. The experimental triáis were performed in nine consecutive days. Each fish was individually placed in the short arm and confined there for thirty seconds, then it was allowed to swim through the aquarium to search for food for ten minutes (máximum period). Time to find food was analysed in seconds. Animáis were injected intraperitoneally with chlorpheniramine (CPA) at 16 mg/kg of body weight or saline after every trial, ten minutes after being placed back in the home aquarium. The results show that all the training latencies of the A-SAL group were higher than the latencies of the S-SAL group. The S-SAL group had decreased latencies from the second trial on, while the S-CPA group showed decreased latencies after the fourth trial. The A-SAL group showed reduced latencies after the fifth trial, but the A-CPA group mainteined the latencies throughout the experiment. This suggests that CPA impairs the consolidation of learning both on telencephalon ablated animáis and in sham-operated ones through its action on mesencephalic structures of the brain and/or on the cerebellum in teleost fish.

Assuntos