RESUMO
The aim of this work was to isolate fungal strains from phytotoxic agricultural soils, screen them, categorize the most tolerant fungi to three fungicides, and identify them by a molecular approach. In this study, 28 fungal strains were isolated from phytotoxic agricultural soil with intensive use of pesticides. The capacity of fungi to resist and degrade different concentrations of carbendazim, captan, and zineb was determined by an exploratory multivariate analysis. Actinomucor elegans LBM 239 was identified as the most tolerant fungus to these fungicides, degrading a 86.62% of carbendazim after 7 days of treatment. In conclusion, A. elegans LBM 239 demonstrated the highest tolerance and capacity to biodegrade carbendazim, becoming a potential candidate for bioremediation of contaminated soils with carbendazim, captan, or zineb.
Assuntos
Fungicidas Industriais , Poluentes do Solo , Zineb , Captana/análise , Fungicidas Industriais/farmacologia , Solo , Microbiologia do Solo , Poluentes do Solo/metabolismoRESUMO
An analytical method has been developed that can be used to determine the potential dermal exposure (PDE) of workers to the pesticide captan in small-scale horticultural production units. The methodology is based on the whole body dosimetry technique, using a cotton coverall and cotton gloves as sampling media, with protective clothing worn beneath the cotton media to protect the operator. The quantitative determination of captan was done by gas chromatography-electron capture detector (GC-ECD), with the analytical method validated by measuring limits of detection and quantification, linear ranges, sample recovery and precision. Special emphasis is placed on factors that affected the stability of captan during chromatographic determination. The data generated for potential dermal exposure are presented separately for mixing/loading and application activities. These data are compared with values obtained with visible tracers using a similar field technique. Margin of safety (MOS) values are also calculated for the agricultural procedures studied.
Assuntos
Agricultura , Captana/análise , Cromatografia Gasosa/métodos , Derme/química , Monitoramento Ambiental/métodos , Fungicidas Industriais/análise , Exposição Ocupacional/análise , Argentina , Humanos , Roupa de Proteção , Medição de Risco/métodos , Espectrofotometria/métodos , VerdurasRESUMO
Resíduos de captan e clorotalonil foram quantificados em morangos "in natura" após três e sete dias de estocagem em geladeira -5oC . As amostras analisadas foram coletadas na CEASA de Campinas e também obtidas após cultivo em estaçäo experimental