RESUMO
Two outbreaks of sheeppox in sheep have occurred in Gansu Province, China. The P32, GPCR, and RPO30 genes were used as markers for differential diagnosis. We confirmed that the outbreaks were caused by sheeppox virus. Sequence and phylogenetic analysis of the P32, GPCR, and RPO30 genes revealed a close relationship between the 2 isolates and Chinese sheeppox viruses. Because ill sheep were imported from Jingyuan, another county of Gansu Province, our results strongly suggest the importance of veterinary surveillance prior to transportation.
Assuntos
Capripoxvirus/genética , Infecções por Poxviridae/veterinária , Doenças dos Ovinos/virologia , Proteínas Virais/genética , Sequência de Aminoácidos , Animais , Capripoxvirus/classificação , Capripoxvirus/isolamento & purificação , China , DNA Viral/genética , Surtos de Doenças , Marcadores Genéticos , Dados de Sequência Molecular , Filogenia , Filogeografia , Alinhamento de Sequência , Ovinos/virologiaRESUMO
An outbreak of sheep pox was investigated in the Ningxia Hui Autonomous Region in China. Through immunofluorescence testing, isolated viruses, polymerase chain reaction identification, and electron microscopic examination, the isolated strain was identified as a sheep pox virus. The virus was identified through sequence and phylogenetic analysis of the P32 gene, open reading frame (ORF) 095, and ORF 103 genes. This study is the first to use the ORF 095 and ORF 103 genes as candidate genes for the analysis of sheep pox. The results showed that the ORF 095 and ORF 103 genes could be used for the genotyping of the sheep pox virus.