RESUMO
Os objetivos deste trabalho foram determinar o tamanho ótimo de parcela e o número de repetições para avaliar caracteres de feijão de porco (Canavalia ensiformis). Foram realizados seis ensaios de uniformidade de 5m×5m (25m2). Cada ensaio foi dividido em 25 unidades experimentais básicas (UEB) de 1m×1m, totalizando 150UEB. Foi pesada a massa verde de vagens (MVV) e a massa verde de parte aérea sem vagens (MVPASV) e calculada a massa verde de parte aérea (MVPA=MVV+MVPASV) das plantas de cada UEB. Foi determinado o tamanho ótimo de parcela e comparadas as médias entre os caracteres MVV, MVPASV e MVPA. Após, foi calculado o número de repetições. Os tamanhos ótimos de parcela para avaliar a massa verde de vagens, a massa verde de parte aérea sem vagens e a massa verde de parte aérea são, respectivamente, 8,59m2, 6,14m2 e 5,85m2. Quatro repetições, para avaliar até 50 tratamentos, no delineamento blocos ao acaso, são suficientes para identificar, como significativas a 5% de probabilidade, pelo teste de Tukey, diferenças entre médias de tratamentos de 55,48%, 39,66% e 37,78% das médias de massa verde de vagens, de massa verde de parte aérea sem vagens e de massa verde de parte aérea do experimento, respectivamente.(AU)
The objectives of this research were to determine the optimum plot size and the number of repetitions to evaluate characters of jack bean (Canavalia ensiformis). It was carried six uniformity assays of size 5m×5m (25m2). Each assay was divided in 25 basic experimental units (UEB) of 1m×1m, totaling 150UEB. The fresh mass of pods (MVV) and fresh mass of aerial part without pods (MVPASV) were weighed and fresh mass of the aerial part (MVPA=MVV+MVPASV) of plants of each UEB was calculated. The optimum plot size was determined and the means were compared, between characters MVV, MVPASV and MVPA. The number of replicates was determined. The optimum plot sizes to evaluate the fresh mass of pods, fresh mass of aerial part without pods and fresh mass of aerial part are, respectively, 8.59m2, 6.14m2 and 5.85m2. Four replications, to evaluate up to 50 treatments, in randomized block designs, are sufficient to identify, as significant at the 5% probability by Tukey test, differences between treatment means 55.48%, 39.66% and 37.78% of the average fresh mass of pods, fresh mass of aerial part without pods and fresh mass of aerial part of the experiment, respectively.(AU)
Assuntos
Canavalia/crescimento & desenvolvimento , Canavalia/fisiologiaRESUMO
Indole-3-acetic acid (IAA) bound is considered a storage molecule and is inactive. However, some studies have proposed an additional possible regulatory mechanism based on the ability of lectins to form complexes with IAA. We report the first crystal structure of ConM in complex with IAA at 2.15 Å resolution. Based on a tetrameric model of the complex, we hypothesize how the lectin controls the availability of IAA during the early seedling stages, indicating a possible new physiological role for these proteins. A free indole group is also bound to the protein. The ConM interaction with different forms of IAA is a strategy to render the phytohormone unavailable to the cell. Thus, this new physiological role proposed for legume lectins might be a novel mechanism by which IAA levels are decreased in addition to the destruction and formation of new complexes in the later stages of seed germination.