Assuntos
Amebíase/diagnóstico , Amebíase/imunologia , Células Clonais/análise , Células Clonais/imunologia , Células Clonais/ultraestrutura , Entamoeba histolytica/análise , Entamoeba histolytica/imunologia , Entamoeba histolytica/ultraestrutura , Ensaio de Imunoadsorção Enzimática/instrumentação , Técnicas In VitroAssuntos
Camundongos , Animais , Amoeba/classificação , Amoeba/patogenicidade , Amoeba/ultraestrutura , Anticorpos Monoclonais/análise , Anticorpos Monoclonais/imunologia , Células Clonais/análise , Quitinases/isolamento & purificação , Quitinases/farmacocinética , Eletroforese , Eletroforese/instrumentaçãoRESUMO
The relationship between acute-phase responses and bacterial properties was studied in a population of 88 children with their first known episode of acute pyelonephritis. One strain from each patient was included in the study. Eighty-four of the patients were infected with Escherichia coli, which was assigned a clonotype according to the O:K:H stereotype; 55 patients carried one of the 12 multiply occurring clones. Globotetraosylceramide-specific (globo+) adhesion was present in 90% of these 12 clones, compared with 62% in the remaining 29 singly occurring clones. The patients infected with globo+ strains had significantly increased inflammatory reactions compared with patients with globo- strains. The O1:K1:H7 strain was the single most frequent clone (n = 14) that always expressed globo+ adhesins. Patients infected with O1:K1:H7 had an inflammatory response similar to that of other globo+ infections, but had a shorter duration of symptoms before diagnosis, higher fever, and higher peripheral leukocyte count. These results demonstrate special virulence of the O1:K1:H7 clone, reflected by the acuteness of onset of infection.
Assuntos
Escherichia coli/genética , Pielonefrite/microbiologia , Criança , Pré-Escolar , Células Clonais/análise , Escherichia coli/patogenicidade , Feminino , Humanos , Lactente , Masculino , Estudos ProspectivosRESUMO
Inhibiçäo do crescimento de um subespécie de Leishmania por exometabólitos de outra subespécie, um fenômeno ainda näo notificado, é sugerido em nossas recentes observaçöes em experimentos de clonagem celular com Leishmania mexicana mexicana e Leishmania mexicana amazonensis. Os clones foram identificados usando a técnica de análise de esquizodemas. O fenômeno observado é claramente relevante em estudos de isolamento parasitário, metabolismo, imunidade cruzada e quimioterapia