RESUMO
TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1-like. Phylogenetic analysis of TFL1 homologous genes revealed that BoTFL1-like shared more than 90% identity with the TFL1 genes of other Gramineae. RT-PCR analysis showed that the expression level of BoTFL1-like in floral buds was almost 3.5 times higher than in vegetative buds. In 35S::BoTFL1-like transgenic Arabidopsis thaliana plants, the time of flowering was significantly delayed by 5 to 9 days, and development of floral buds and sepals was severely affected compared to wild type Arabidopsis plants. This suggests that the BoTFL1-like gene may play roles in flowering time and flower morphological structure in B. oldhamii. The BoTFL1-like gene driven by the 35S promoter almost fully rescued the phenotype of the tfl1 mutant apart from the number of rosette inflorescences, indicating that the function of BoTFL1-like was similar to TFL1 in Arabidopsis. We conclude the TFL1 gene function has been conserved between B. oldhamii and A. thaliana.
Assuntos
Arabidopsis/genética , Bambusa/genética , Expressão Ectópica do Gene , Flores/genética , Mutação , Fenótipo , Proteínas de Plantas/genética , Sequência de Aminoácidos , Bambusa/classificação , Sequência de Bases , Clonagem Molecular , Modelos Moleculares , Dados de Sequência Molecular , Filogenia , Proteínas de Plantas/química , Conformação Proteica , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
The Neotropical woody bamboo genus Otatea is one of five genera in the subtribe Guaduinae. Of the eight described Otatea species, seven are endemic to Mexico and one is also distributed in Central and South America. Otatea acuminata has the widest geographical distribution of the eight species, and two of its recently collected populations do not match the known species morphologically. Parametric and non-parametric methods were used to delimit the species in Otatea using five chloroplast markers, one nuclear marker, and morphological characters. The parametric coalescent method and the non-parametric analysis supported the recognition of two distinct evolutionary lineages. Molecular clock estimates were used to estimate divergence times in Otatea. The results for divergence time in Otatea estimated the origin of the speciation events from the Late Miocene to Late Pleistocene. The species delimitation analyses (parametric and non-parametric) identified that the two populations of O. acuminata from Chiapas and Hidalgo are from two separate evolutionary lineages and these new species have morphological characters that separate them from O. acuminata s.s. The geological activity of the Trans-Mexican Volcanic Belt and the Isthmus of Tehuantepec may have isolated populations and limited the gene flow between Otatea species, driving speciation. Based on the results found here, I describe Otatea rzedowskiorum and Otatea victoriae as two new species, morphologically different from O. acuminata.
Assuntos
Bambusa/classificação , Bambusa/anatomia & histologia , Bambusa/genética , Teorema de Bayes , DNA de Cloroplastos/genética , Ecossistema , Fluxo Gênico , Especiação Genética , Funções Verossimilhança , México , Filogenia , Análise de Sequência de DNA , Estatísticas não ParamétricasRESUMO
La presente nota da a conocer las especies de Poaceae (Gramineae) bambusiformes presentes en y alrededor de la concesión para conservación del Rió de Los Amigos en la cuenca del Madre de Dios, sur de la Amazonia peruana. Proporciona claves y material grafico para la identificación de los taxones presentes en la zona. (au)
This note offers to know the species of bambusiform Poaceae (Gramineae) present in and around the Los Amigos River Concession for Conservation, in the Madre de Dios, basin the southern part of the Peruvian Amazonia. It supply keys and illustrated material for identification of the taxa present within the area.