RESUMO

Pure cultures of Thiobacillus ferrooxidans and mixed cultures of Thiobacillus ferrooxidans and Leptospirillum ferrooxidans isolated from the Matahambre mine (Cuba) were used to fit immunodiffusion and immunoelectron microscopy to the study of iron oxidizing bacteria. The possibilities, advantages and limits of those techniques have been studied from both the identification and the serological characterization points of view. Finally, the efficiency of these methods was tested by applying them to the identification of microorganisms from acidic waters from the mine.

Assuntos

Imunodifusão , Ferro/metabolismo , Microscopia Imunoeletrônica , Microbiologia do Solo , Thiobacillus/classificação , Animais , Anticorpos Antibacterianos/imunologia , Bactérias Gram-Negativas Quimiolitotróficas/classificação , Bactérias Gram-Negativas Quimiolitotróficas/imunologia , Bactérias Gram-Negativas Quimiolitotróficas/isolamento & purificação , Bactérias Gram-Negativas Quimiolitotróficas/metabolismo , Coelhos , Thiobacillus/imunologia , Thiobacillus/isolamento & purificação , Thiobacillus/metabolismo