RESUMO
Botanical drugs are complementary therapies in the management of diabetes mellitus. In this work, we studied the effects of chronic treatment of diabetic rats with A. indica (neem) on blood glucose, pancreatic islet histopathology, and oxidative status of the pancreas. Fifty-four Wistar rats (5-8 weeks old) were randomly assigned to 5 treatment groups. Hyperglycemia was induced in 34 fasted rats with a single i.p. injection of STZ (70 mg/kg bw/d). Ethanolic extract of A. indica leaves (500 mg/kg bw/d) was given orally to diabetic rats (n=12) for 50d. Glibenclamide was given (p.o) at 600 µg/ kg bw/d. In each group, blood glucose, islet histopathology, and pancreatic oxidative status, were assessed. All hyperglycemic rats in the neem-treated group had become normoglycemic at the end of week 2. By 50d, the number of viable b cells was highest in the neem-treated diabetic rats (compared with the diabetic and glibenclamide groups). Similarly, islet histology showed marked improvement in this group, in addition to improved oxidative stress. Our findings confirmed the hypoglycemic effect of neem. Besides, the improved islet morphology and oxidative status in neem-treated diabetic rats suggest the potential of this herb at improving lesions of the pancreatic islet in diabetes mellitus.
Los medicamentos a base de plantas son terapias complementarias en el manejo de la diabetes mellitus. En este trabajo se estudiaron los efectos del tratamiento crónico de ratas diabéticas con A. indica (Neem) sobre la glucosa de la sangre, la histopatología de los islotes pancreáticos, y el estado oxidativo del páncreas. Cincuenta y cuatro ratas Wistar (5-8 semanas de edad) fueron asignadas aleatoriamente a 5 grupos de tratamiento. La hiperglucemia fue inducida en 34 ratas en ayunas con una única inyección IP de STZ (70 mg/kg peso corporal/d). El extracto etanólico de hojas de A. indica (500 mg/kg de peso corporal/día) fue administrado por vía oral a ratas diabéticas (n=12) por 50d. Glibenclamida fue dada (PO) a 600 mg/kg peso corporal/d. En cada grupo, la glucosa en la sangre, la histopatología de los islotes, y el estado oxidativo de páncreas, se evaluaron. Todas las ratas de hiperglucemia en el grupo tratado con el Neem se habían convertido en normoglucémicas al final de la semana 2. Por 50d, el número de células b viables fue mayor en el Neem ratas tratadas con diabetes (en comparación con los grupos de diabéticos y glibenclamida). Del mismo modo, la histología de los islotes mostró una notable mejoría en este grupo, además de mejorar el estrés oxidativo. Nuestros resultados confirman el efecto hipoglucemiante de Neem. Además, la mejora de la morfología de los islotes y el estado de oxidación en el neem tratados con ratas diabéticas sugieren el potencial de esta hierba en la mejora de las lesiones de los islotes pancreáticos en la diabetes mellitus.
Assuntos
Animais , Ratos , Azadirachta/uso terapêutico , Diabetes Mellitus Experimental/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Ilhotas Pancreáticas , Azadirachta/farmacologia , Diabetes Mellitus/tratamento farmacológico , Extratos Vegetais/farmacologia , Glibureto/uso terapêutico , Glicemia , Hipoglicemiantes , Ilhotas Pancreáticas/patologia , Peróxido de Hidrogênio/análise , Peróxidos Lipídicos/análise , Peso Corporal , Ratos Wistar , Superóxido Dismutase , Fatores de TempoRESUMO
This study was carried out with two groups of bovines naturally infected with Boophilus microplus. One group was treated every week during a month with Neem's watery extract and the other group was treated only once (at the test onset) with amabectin pour-on. The results of the Kruskal-Wallis test showed no significant difference (P<0.0001) between both groups (Neem and amabectin) throughout the experiment. However, time of collection [(day0 = day15) Õ day30] and age brackets (cowsAssuntos
Animais
, Azadirachta/administração & dosagem
, Azadirachta/farmacologia
, Bovinos/parasitologia
, Controle de Ácaros e Carrapatos/métodos
, Plantas Medicinais
RESUMO
This study was carried out with two groups of bovines naturally infected with Boophilus microplus. One group was treated every week during a month with Neem's watery extract and the other group was treated only once (at the test onset) with amabectin pour-on. The results of the Kruskal-Wallis test showed no significant difference (P<0.0001) between both groups (Neem and amabectin) throughout the experiment. However, time of collection [(day0 = day15) Õ day30] and age brackets (cowsAssuntos
Animais
, Azadirachta/administração & dosagem
, Azadirachta/farmacologia
, Controle de Ácaros e Carrapatos/métodos
, Plantas Medicinais
, Bovinos/parasitologia
RESUMO
Effect of oral administration of crude aqueous neem extract on serum testosterone and other blood constituents was studied in the male Wistar rats for 10 weeks. The neem treatment resulted in significant decreases (p,0.01) in total testosterone, total bilirubin and K+ in serum. There were also increases (p<0.05) in packed cell volume, mean corpuscular haemoglobin concentration, red blood cell, white blood cell and lymphocyte counts without showing any cytotoxic effects in the body (AU)