RESUMO

The identification of chlorophyll molecules with peroxyl radical scavenger capacity in microalgae Phormidium autumnale was determined. The ultrasound-assisted extraction was utilized for obtaining the chlorophyll compounds from biomass. A total of eleven molecules were separated in microalgae chlorophyll extract, with pheophytin a' (371µg·g-1) and chlorophyll a (159.3µg·g-1) as the major ones. The chlorophyll extract was shown to be a potent scavenger of peroxyl radical, being almost 200 times more potent than α-tocopherol. These facts suggest the microalgae Phormidium autumnale as potential source of bioactive tetrapyrrole compounds.

Assuntos

Asphodelaceae/metabolismo , Clorofila/farmacologia , Manipulação de Alimentos/métodos , Sequestradores de Radicais Livres/farmacologia , Microalgas/metabolismo , Peróxidos/química , Ultrassom , Clorofila/isolamento & purificação , Clorofila A/isolamento & purificação , Clorofila A/farmacologia , Cromatografia Líquida de Alta Pressão , Sequestradores de Radicais Livres/isolamento & purificação , Microalgas/crescimento & desenvolvimento , Feofitinas/isolamento & purificação , Feofitinas/farmacologia , Espectrometria de Massas em Tandem , Asphodelaceae/crescimento & desenvolvimento , alfa-Tocoferol/farmacologia