RESUMO
Chlorhexidine digluconate (CHX) is a gold standard drug in dentistry and is widely used as a reference in both
Gluconato de clorexidina (CHX) é um fármaco considerado padrão ouro, em Odontologia, amplamente usado como referência em estudos
Assuntos
Humanos , Antifúngicos/análise , Antifúngicos/farmacocinética , Antissépticos Bucais/análise , Antissépticos Bucais/farmacocinética , Produtos para Higiene Dental e BucalRESUMO
Plaque fluoride concentrations ([F]) are directly related to plaque calcium concentrations [Ca]. Attempts to increase plaque F uptake from dentifrices or rinses have used methods designed to increase plaque [Ca] but with inconsistent results. This double-blind, double-crossover study tested the effect of a 150 mM calcium lactate rinse used prior to brushing with placebo or fluoridated dentifrices (1030 p.p.m. as NaF) on plaque and salivary [F] and [Ca]. Sixteen children (8-10 yr of age) were randomly assigned to four different groups according to the four treatments (placebo dentifrice or fluoridated dentifrice preceded by calcium lactate or deionized water prerinses). Plaque and saliva were collected 1 and 12 h after brushing on day 7 after starting to use the dentifrices. F was determined using the electrode and Ca was determined using atomic absorption spectrometry. Plaque and salivary [Ca] were not significantly increased after use of the calcium lactate prerinse, except for plaque [Ca] 1 h after the use of the placebo dentifrice. A significant increase in salivary [F] was associated with the calcium lactate prerinse only at 1 h after the use of the fluoridated dentifrice. The the calcium lactate prerinse did not significantly affect plaque [F] under any condition.
Assuntos
Compostos de Cálcio/farmacocinética , Cariostáticos/uso terapêutico , Placa Dentária/prevenção & controle , Dentifrícios/uso terapêutico , Lactatos/farmacocinética , Antissépticos Bucais/farmacocinética , Fluoreto de Sódio/uso terapêutico , Cálcio/análise , Criança , Estudos Cross-Over , Placa Dentária/química , Placa Dentária/metabolismo , Método Duplo-Cego , Fluoretos/análise , Humanos , Modelos Lineares , Saliva/químicaRESUMO
The aim of this study was to quantify the amount of delmopinol recovered in different molecular weight ranges of salivary proteins in the supernatant and pellet of delmopinol-saliva mixes. Unstimulated whole saliva was collected from 5 subjects and mixed with radiolabeled delmopinol to obtain a final drug concentration of 9.7 mM. The salivary-delmopinol solutions were incubated and then centrifuged. The resulting pellets and supernatants were studied in an electrophoresis assay. Each individual sample was run in three different electrophoretic gel lanes. The first lane was stained with silver dyes; the second lane was used for electrophoretic blotting and autoradiography; the third lane was cut in standard slices that were dissolved and analyzed with scintillation counting. The scintillation results demonstrated that higher radioactivity levels were detected at high molecular weight ranges (700-600 kDa). Furthermore, pellet samples were found to contain the highest amounts of delmopinol. The autoradiography results confirmed that delmopinol was bound to proteins of high molecular weight (700-600 kDa).
Assuntos
Depósitos Dentários/metabolismo , Morfolinas/farmacocinética , Antissépticos Bucais/farmacocinética , Proteínas e Peptídeos Salivares/metabolismo , Adulto , Autorradiografia , Eletroforese em Gel de Poliacrilamida , Humanos , Pessoa de Meia-Idade , Peso Molecular , Ligação Proteica , Saliva/metabolismo , Contagem de CintilaçãoRESUMO
The aim of this study was to evaluate, using scanning electron microscopy (SEM), the calcium fluoride uptake by human enamel in situ after topical application using three fluoridated mouthrinses: a neutral pH solution (Fluordent), an acidulated solution (Fluorgard) and a solution associated with chlorhexidine (Duplak). Twenty-four samples from 6 third sound human molars were divided into two groups: 5-min treatment and 10-min treatment. In both the 5-min and 10-min treatment samples, those treated with the chlorhexidine-associated solution showed higher formation of CaF2-like material. In the 10-min treatment group, the samples treated with the neutral solution showed higher formation of CaF2. These results indicated that the products used deposited CaF2 on the enamel surface and may be recommended to aid the prevention and control of dental caries.
Assuntos
Fluoreto de Cálcio/metabolismo , Cariostáticos/farmacocinética , Esmalte Dentário/metabolismo , Antissépticos Bucais/farmacocinética , Adsorção , Humanos , Microscopia Eletrônica de Varredura , Dente Serotino , Propriedades de SuperfícieRESUMO
The clearance pattern of a specific substance is very important to estimate its oral availability. Devices or models that simulate clearance in the mouth are important to study the effects and retention time of foods and drugs. This report describes an efficient device which was assembled with low-cost materials in our laboratory and that can be used to study the clearance of cariogenic substrates, mouthwashes, programmed-release drugs as well as adsorption of drugs to enamel. The device can have up to three chambers with varying minimum and maximum volumes that can be eluted simultaneously at different flows. The simulated swallowed volumes are adjustable and the ratio between the maximum and minimum volumes can be programmed. We also present the results of an evaluation study using the device to determine the clearance of fluoride from a fluoride-containing mouthwash, the clearance of a 1% glucose solution and the programmed release of fluoride from a methacrylate bioadhesive using artificial saliva as eluent.