RESUMO
The aim of this study was to compare the number of CD57+ natural killer (NK) cells and CD8+ T lymphocytes between periapical granulomas (PGs) and radicular cysts (RCs). Twenty-fives cases of PGs and 25 of RCs were submitted to histological analysis and immunohistochemistry using anti-CD57 and anti-CD8 biomarkers. Positive cells were counted in 10 fields (400× magnification) and the median value was calculated for each case. Statistical tests were used to evaluate differences in the number of CD57+ NK cells and CD8+ T lymphocytes according to type of lesion, intensity of the infiltrate and thickness of the lining epithelium. The number of CD57+ NK cells and CD8+ T lymphocytes was higher in PGs than in RCs (p = 0.129 and p = 0.541, respectively). Comparison of the number of CD57+ NK cells in atrophic and hyperplastic epithelium revealed a larger number of cells in the atrophic epithelium (p = 0.042). A larger number of CD57+ NK cells and CD8+ T lymphocytes were observed in grade III infiltrates compared to grade I/II (p = 0.145 and p = 0.725, respectively). CD8+ T lymphocytes were more prevalent than CD57+ NK cells in most cases when PGs and RCs were analyzed separately or in combination (p < 0.0001). CD57+ NK cells and CD8+ T lymphocytes play a key role in antiviral defense and the presence of these cells supports evidence suggesting the participation of these microorganisms in the pathogenesis of PGs and RCs. The response mediated by CD8+ T lymphocytes was more frequent, indicating greater participation of the adaptive immunity in these chronic lesions.
Assuntos
Antígenos CD57/análise , Linfócitos T CD8-Positivos/patologia , Células Matadoras Naturais/patologia , Granuloma Periapical/patologia , Cisto Radicular/patologia , Adolescente , Adulto , Idoso , Biomarcadores/análise , Contagem de Células , Epitélio , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Granuloma Periapical/imunologia , Cisto Radicular/imunologia , Valores de Referência , Índice de Gravidade de Doença , Estatísticas não Paramétricas , Adulto JovemRESUMO
Peri-implantitis is an infectious disease characterized by inflammation of the tissues surrounding the implant, bleeding on probing with or without suppuration, and bone loss. Peri-implant lesions contain a leukocyte infiltrate of plasma cells, lymphocytes, macrophages and neutrophils. A survey of the literature did not show any studies reporting an association between hypoxia and peri-implantitis. The aim of the present cross-sectional study was to evaluate histological changes and immunostaining for CD15, CD57 and HIF-1α in the peri-implant mucosa of patients with and without peri-implantitis. Mucosal biopsies were obtained from 18 patients with peri-implantitis and 10 control subjects without peri-implantitis at a private health care center between 2010 and 2012. The sections were fixed in 10% buffered formalin, processed and embedded in paraffin for histopathological and immunohistochemical study. Acanthosis, spongiosis and exocytosis were observed in both groups, with no significant difference between them. The peri-implantitis group showed increased immunostaining for CD15, a neutrophil marker, and HIF-1α, a tissue hypoxia marker, but no significant difference in immunostaining for CD57, a Natural Killer cell marker. The increase in neutrophil (CD15) and hypoxia (HIF-1α) markers in patients with peri-implantitis suggests an active participation of neutrophils and hypoxia in the pathogenesis of this disease. Since the present study was the first to evaluate the expression of CD15, CD57 and HIF-1α in peri-implant tissues, further studies should be performed to better understand the role of these molecules in peri-implantitis.
Assuntos
Implantes Dentários/efeitos adversos , Peri-Implantite/imunologia , Estomatite/imunologia , Idoso , Biomarcadores/análise , Biópsia , Antígenos CD57/análise , Antígenos CD57/biossíntese , Estudos Transversais , Feminino , Fucosiltransferases/análise , Fucosiltransferases/biossíntese , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/análise , Subunidade alfa do Fator 1 Induzível por Hipóxia/biossíntese , Antígenos CD15/análise , Antígenos CD15/biossíntese , Masculino , Pessoa de Meia-IdadeRESUMO
TNF-α, IFN-γ, IL-10, IL-17, CD68 and CD57 were evaluated in biopsies of patients with American cutaneous leishmaniasis living in Sorocaba, Brazil. The analyses were performed considering the time of lesions from 23 patients with recent lesions (Group I) and 19 patients with late lesions (Group II). All patients were infected with Leishmania (Viannia) braziliensis. Immunostaining cells for CD68, CD57, TNF- α, IFN-γ, IL-10 and IL-17 were performed by immunohistochemistry. Except for CD68 and IL-17, the distribution of in situ for CD57, IL-10, TNF-α and IFN-γ showed that patients with recent lesions expressed higher levels than those with late lesions. The comparison of cytokine expression/group showed that IL-10 was significantly higher than IL-17 and IFN-γ (similar data were shown in IL-17 compared with TNF-α), suggesting an immunological balance between inflammatory-anti-inflammatory agents. This balance was similar for two groups of patients. In conclusion, these data suggested that (i) patients from Group I had recent lesions (in the beginning of chronic phase) compared to those from Group II and (ii) the modulation of inflammatory response in patients with recent American cutaneous leishmaniasis was correlated with IL-10 expression in skin lesions preventing the development of mucosal forms. The parasite treatment also prevented the evolution of severe forms.
Assuntos
Citocinas/imunologia , Leishmania braziliensis/fisiologia , Leishmaniose Cutânea/imunologia , Leishmaniose Cutânea/patologia , Adulto , Antígenos CD/análise , Antígenos CD/imunologia , Antígenos de Diferenciação Mielomonocítica/análise , Antígenos de Diferenciação Mielomonocítica/imunologia , Brasil , Antígenos CD57/análise , Antígenos CD57/imunologia , Citocinas/análise , Feminino , Humanos , Leishmaniose Cutânea/diagnóstico , Leishmaniose Cutânea/parasitologia , Masculino , Pessoa de Meia-IdadeRESUMO
Abstract: The aim of this study was to compare the number of CD57+ natural killer (NK) cells and CD8+ T lymphocytes between periapical granulomas (PGs) and radicular cysts (RCs). Twenty-fives cases of PGs and 25 of RCs were submitted to histological analysis and immunohistochemistry using anti-CD57 and anti-CD8 biomarkers. Positive cells were counted in 10 fields (400× magnification) and the median value was calculated for each case. Statistical tests were used to evaluate differences in the number of CD57+ NK cells and CD8+ T lymphocytes according to type of lesion, intensity of the infiltrate and thickness of the lining epithelium. The number of CD57+ NK cells and CD8+ T lymphocytes was higher in PGs than in RCs (p = 0.129 and p = 0.541, respectively). Comparison of the number of CD57+ NK cells in atrophic and hyperplastic epithelium revealed a larger number of cells in the atrophic epithelium (p = 0.042). A larger number of CD57+ NK cells and CD8+ T lymphocytes were observed in grade III infiltrates compared to grade I/II (p = 0.145 and p = 0.725, respectively). CD8+ T lymphocytes were more prevalent than CD57+ NK cells in most cases when PGs and RCs were analyzed separately or in combination (p < 0.0001). CD57+ NK cells and CD8+ T lymphocytes play a key role in antiviral defense and the presence of these cells supports evidence suggesting the participation of these microorganisms in the pathogenesis of PGs and RCs. The response mediated by CD8+ T lymphocytes was more frequent, indicating greater participation of the adaptive immunity in these chronic lesions.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Idoso , Adulto Jovem , Granuloma Periapical/patologia , Células Matadoras Naturais/patologia , Cisto Radicular/patologia , Linfócitos T CD8-Positivos/patologia , Antígenos CD57/análise , Granuloma Periapical/imunologia , Valores de Referência , Índice de Gravidade de Doença , Imuno-Histoquímica , Biomarcadores/análise , Cisto Radicular/imunologia , Contagem de Células , Estatísticas não Paramétricas , Epitélio , Pessoa de Meia-IdadeRESUMO
BACKGROUND AND OBJECTIVE: The periodontal ligament is a specialized connective tissue, derived from dental follicle and originated from neural crest cells. Recently it has been suggested, based on animal models, that periodontal ligament could be a niche for neural crest stem cells. However, there is still little knowledge on this subject. The identification of neural crest adult stem cells has received much attention based on its potential in tissue regeneration. The objective of the present work was to verify the human periodontal ligament as a niche for neural crest stem cells. MATERIAL AND METHODS: Cells from human periodontal ligament were isolated from 10 teeth of seven individuals (periodontal ligament pool group) and also from four teeth of one individual (periodontal ligament single group), after enzymatic digestion. The cells were cultured in specific inductive medium. Analyses of protein and gene expression were performed through immunocytochemistry and reverse transcription-polymerase chain reaction techniques, respectively. RESULTS: Mesodermal phenotypes (adipogeneic, osteogenic and myofibroblastic) were identified after culture in inductive medium. Immunocytochemistry analyses showed the presence of the nestin marker of neural stem cells and also markers of undifferentiated neural crest cells (HNK1, p75). When cultured in inductive medium that allowed neural differentiation, the cells showed markers for beta-tubulin III, neurofilament M, peripherin, microtubule-associated protein 2 and protein zero. The results were similar between the two study groups (the periodontal ligament pool group and the periodontal ligament single group). CONCLUSION: This research provides evidence that human periodontal ligament, in addition to its mesodermal derivatives, produces neural crest-like cells. Such features suggest a recapitulation of their embryonic state. The human periodontal ligament revealed itself as a viable alternative source for possible primitive precursors to be used in stem-cell therapies.
Assuntos
Células-Tronco Adultas , Crista Neural/citologia , Ligamento Periodontal/citologia , Adolescente , Adulto , Antígenos CD57/análise , Linhagem da Célula , Células Cultivadas , Perfilação da Expressão Gênica , Humanos , Imuno-Histoquímica , Proteínas de Filamentos Intermediários/análise , Glicoproteínas de Membrana/análise , Proteínas Associadas aos Microtúbulos/análise , Proteína P0 da Mielina/análise , Proteínas do Tecido Nervoso/análise , Nestina , Proteínas de Neurofilamentos/análise , Ligamento Periodontal/química , Periferinas , Tubulina (Proteína)/análise , Adulto JovemRESUMO
This study evaluated the presence and density of natural killer (NK) cells as well as collagen density in chronic apical periodontitis lesions and tried to find any correlations with concomitant herpesvirus infection or histopathological status of the lesion. Surgical specimens of chronic apical periodontitis lesions were surveyed for the presence and density of NK cells by immunohistochemical analysis. Collagen density in these lesions was quantified by means of histochemistry. All specimens were positive for the presence of CD57-positive cells. Topographically, CD57-positive cells were found singly or forming clusters in the granulomatous tissue, as well as subjacent and within the cystic epithelium. No significant differences in the density of CD57-positive cells were found between nonepithelialized and epithelialized lesions or between herpesvirus-positive and herpesvirus-negative lesions. Significant differences were found in volumetric density of collagen when comparing nonepithelialized and epithelialized lesions, with the latter demonstrating higher values. When no distinction of lesion type was made, there was no significant difference in collagen density between herpesvirus-positive and herpesvirus-negative lesions. When comparing the collagen density in herpesvirus-positive and herpesvirus-negative specimens from the same lesion type, a significant difference was found in nonepithelialized lesions, with herpesvirus-positive lesions showing lower values. The presence of CD57-positive cells in all chronic apical periodontitis specimens may indicate that activated NK cells play a role in the pathogenesis of this disease, possibly by participating in innate immunity events involved in the control of virus infection. Collagen density may vary in function of the type of lesion and presence of herpesvirus infection.
Assuntos
Colágeno/ultraestrutura , Infecções por Herpesviridae/diagnóstico , Células Matadoras Naturais/patologia , Periodontite Periapical/virologia , Antígenos CD57/análise , Contagem de Células , Doença Crônica , Infecções por Citomegalovirus/diagnóstico , Epitélio/patologia , Epitélio/virologia , Infecções por Vírus Epstein-Barr/diagnóstico , Infecções por Herpesviridae/imunologia , Infecções por Herpesviridae/patologia , Humanos , Imunidade Inata/imunologia , Imuno-Histoquímica , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/virologia , Granuloma Periapical/imunologia , Granuloma Periapical/patologia , Granuloma Periapical/virologia , Periodontite Periapical/imunologia , Periodontite Periapical/patologia , Tecido Periapical/patologia , Tecido Periapical/virologia , Cisto Radicular/imunologia , Cisto Radicular/patologia , Cisto Radicular/virologiaAssuntos
Biomarcadores Tumorais/análise , Neoplasias da Mama/patologia , Tumor de Células Granulares/patologia , Idoso , Antígenos CD/análise , Antígenos de Diferenciação Mielomonocítica/análise , Neoplasias da Mama/química , Antígenos CD57/análise , Calbindina 2 , Feminino , Tumor de Células Granulares/química , Humanos , Imuno-Histoquímica , Inibinas/análise , Antígeno Ki-67/análise , Mamografia/métodos , Proteínas Proto-Oncogênicas c-bcl-2/análise , Proteína G de Ligação ao Cálcio S100/análise , Proteínas S100/análise , Ubiquitina Tiolesterase/análiseRESUMO
OBJECTIVE: To investigate infiltrating cells in the liver of children with type 1 autoimmune hepatitis (AH-1). METHODS: liver biopsies from 24 untreated AH-1 patients (14 children, 10 adults), five patients with hepatitis C virus related chronic hepatitis (HCV), and 10 control liver specimens (CL) were processed for immunohistochemical cell characterisation. RESULTS: Two different cell distribution patterns were detected in the liver of patients with AH-1: (1) CD4(+) and CD20(+) cells were found in the central areas of the portal tracts (portal distribution); (2) CD8(+) cells were observed at the periphery of the portal space (periportal distribution). Some cell subsets, like CD56, CD57, Fas-L, and Bak, showed a non-defined distribution pattern. The presence of two well defined patterns of cell distribution was not observed in HCV and CL (CD4(+), CD20(+), and CD8(+) cells were uniformly distributed in the portal space). In AH-1 and CL, the NK markers CD56 and CD57 were found scattered throughout the liver parenchyma. However, in HCV biopsies, CD56(+) cells were also clearly increased in both the portal and the periportal areas. Biopsies of AH-1 and HCV patients showed a uniform distribution of Fas-L and Bak in the portal and periportal areas, with Bak staining also detected in the hepatic parenchyma. CONCLUSIONS: Despite clinical and genetic differences, there was a similar distribution of liver infiltrating mononuclear cells in children and adults with AH-1. These results raise the possibility of reclassifying cryptogenic chronic hepatitis by immunohistochemical analysis of infiltrating liver cells.
Assuntos
Hepatite Autoimune/imunologia , Leucócitos Mononucleares/imunologia , Fígado/imunologia , Linfócitos T CD4-Positivos/imunologia , Antígeno CD56/análise , Antígenos CD57/análise , Linfócitos T CD8-Positivos/imunologia , Estudos de Casos e Controles , Distribuição de Qui-Quadrado , Criança , Pré-Escolar , Proteína Ligante Fas , Feminino , Hepacivirus , Hepatite C Crônica/imunologia , Humanos , Imuno-Histoquímica/métodos , Imunofenotipagem/métodos , Masculino , Glicoproteínas de Membrana/análise , Fatores de Necrose Tumoral/análise , Proteína Killer-Antagonista Homóloga a bcl-2/análiseRESUMO
OBJECTIVE: The purpose of the present study was to evaluate the relationship between histological epithelial dysplasia, the immunolocalization of p53 and glutathione S-transferase pi on the immunolocalization of the CD57 antigen. MATERIALS AND METHODS: Seventy biopsies were included in the study and the streptavidin-biotin-peroxidase method was used to detect the antigens. RESULTS: The results demonstrated a significant relationship between p53, GST-pi positive staining with moderate/severe epithelial dysplasia. There was no relationship between p53 and GST-pi. The mean number of CD57+ lymphoid cells was higher in the lesions with increased epithelial dysplasia and positive for GST-pi. No difference was found regarding CD57 immunolocalization in leukoplakias positive and negative for p53. CONCLUSIONS: As the presence of CD57+ lymphoid cells are indicative of immunosuppression, our study suggests that the severity of epithelial dysplasia and positive immunolabelling for GST-pi are associated with local immune response alterations in oral leukoplakias. Our data also give support to the idea that GST-pi and p53 are not time-point related during oral cancer development.