RESUMO
This study assessed and compared different methods for vegetative propagation of a miniature ornamental pineapple hybrid (ORN-MUT), seeking to determine the best method for production of plantlets, as well as for removal of the PMWaV viral complex from plants cultured in vitro, for production of healthy parent plants. Pineapple wilt is a disease that can cause large economic and is caused by a viral complex called Pineapple mealybug wilt-associated virus (PMWaV). For this, four propagation methods were evaluated (conventional, stem sectioning, micropropagation and etiolation of nodal segments). The time necessary for each method and the number of plants formed were assessed. Stem tips (0.5 mm) were cultured and indexed for three PMWaV types. Conventional propagation produced 17 plantlets per plant in 566 days, stem sectioning produced 2.3 plantlets per stem in 591 days, while the conventional micropropagation technique produced 1,284 plants after four subcultures in 778 days. Stems etiolated for 60 days showed peak production in the second subculture, with 1,224 plants. This method required 883 days to obtain plants with ideal size for transplantation to the field. In turn, stems etiolated for 120 days produced 935 plants at the end of four subcultures, with peak output in the third subculture, in which the plants could be cultivated in the field after 943 days. Conventional micropropagation and etiolation for 60 days were the best methods for production of plantlets of the ORN-MUT hybrid. The results of this work showed that the cultivation of shoot tips is an efficient strategy to remove the PMWaV complex and obtain healthy mother plants and can be a useful tool for other varieties of pineapple.
Assuntos
Ananas/crescimento & desenvolvimento , Ananas/virologia , EstiolamentoRESUMO
This study assessed and compared different methods for vegetative propagation of a miniature ornamental pineapple hybrid (ORN-MUT), seeking to determine the best method for production of plantlets, as well as for removal of the PMWaV viral complex from plants cultured in vitro, for production of healthy parent plants. Pineapple wilt is a disease that can cause large economic and is caused by a viral complex called Pineapple mealybug wilt-associated virus (PMWaV). For this, four propagation methods were evaluated (conventional, stem sectioning, micropropagation and etiolation of nodal segments). The time necessary for each method and the number of plants formed were assessed. Stem tips (0.5 mm) were cultured and indexed for three PMWaV types. Conventional propagation produced 17 plantlets per plant in 566 days, stem sectioning produced 2.3 plantlets per stem in 591 days, while the conventional micropropagation technique produced 1,284 plants after four subcultures in 778 days. Stems etiolated for 60 days showed peak production in the second subculture, with 1,224 plants. This method required 883 days to obtain plants with ideal size for transplantation to the field. In turn, stems etiolated for 120 days produced 935 plants at the end of four subcultures, with peak output in the third subculture, in which the plants could be cultivated in the field after 943 days. Conventional micropropagation and etiolation for 60 days were the best methods for production of plantlets of the ORN-MUT hybrid. The results of this work showed that the cultivation of shoot tips is an efficient strategy to remove the PMWaV complex and obtain healthy mother plants and can be a useful tool for other varieties of pineapple.(AU)
Assuntos
Ananas/crescimento & desenvolvimento , Ananas/virologia , EstiolamentoRESUMO
O objetivo desse estudo foi avaliar a influência do ponto de colheita na qualidade sensorial e físico-química de abacaxi FRF632. Os frutos foram avaliados por 59 julgadores à cor, aroma, sabor, textura/firmeza, aceitação global e intenção de compra. Avaliou-se também a acidez titulável, o teor de sólidos solúveis e a relação sólidos solúveis/acidez titulável. Os consumidores gostaram dos frutos, com percentual de aprovação superior a 75% para todos os atributos. A doçura e a acidez dos frutos, nos estádios de maturação amarelo e colorido, foram consideradas ideias por um maior número de consumidores. Conclui-se que os consumidores preferiram consumir frutos colhidos nos estádios de maturação colorido e amarelo, pois os consideraram com melhor cor, sabor e aroma, doçura e acidez ideais, além de maior intenção de compra.
Assuntos
Humanos , Ananas/crescimento & desenvolvimento , Ananas/química , Comportamento do Consumidor/estatística & dados numéricos , Produção Agrícola , Fatores de Tempo , Fenômenos Químicos , Fusarium , PercepçãoRESUMO
O objetivo desse estudo foi avaliar a influência do ponto de colheita na qualidade sensorial e físico-química de abacaxi FRF632. Os frutos foram avaliados por 59 julgadores à cor, aroma, sabor, textura/firmeza, aceitação global e intenção de compra. Avaliou-se também a acidez titulável, o teor de sólidos solúveis e a relação sólidos solúveis/acidez titulável. Os consumidores gostaram dos frutos, com percentual de aprovação superior a 75% para todos os atributos. A doçura e a acidez dos frutos, nos estádios de maturação amarelo e colorido, foram consideradas ideias por um maior número de consumidores. Conclui-se que os consumidores preferiram consumir frutos colhidos nos estádios de maturação colorido e amarelo, pois os consideraram com melhor cor, sabor e aroma, doçura e acidez ideais, além de maior intenção de compra.(AU)
Assuntos
Humanos , Ananas/química , Ananas/crescimento & desenvolvimento , Fatores de Tempo , 24444 , Comportamento do Consumidor/estatística & dados numéricos , Fenômenos Químicos , Percepção , FusariumRESUMO
There is a lack of information regarding the use of herbicides in pineapple crop, so the aim was to evaluate the growth and initial development of 'Smooth Cayenne' and 'Pérola' pineapple seedlings under application of diuron, sulfentrazone and ametryn, isolated and associated, in post-planting. The experiments were conducted in a greenhouse and the treatments consisted on application of herbicides after planting. Crop injury, leaf length,dry leaf mass and total aerial part were evaluated. The initial growth and development of the 'Smooth Cayenne' pineapple seedlings were not negatively influenced by the application of diuron. The initial growth and development of pineapple seedlings 'Pérola' was not negatively influenced by the application of diuron and ametryn, isolated or associated.
Há déficit de informações a respeito do uso de herbicidas no cultivo de abacaxi, assim objetivou-se avaliar o crescimento e desenvolvimento inicial de mudas de abacaxi Smooth Cayenne e Pérola sob aplicação de diuron, sulfentrazone e ametryn, isolados e em associações, em pós-plantio. Os experimentos foram conduzidos em casa de vegetação e os tratamentos foram compostos pela aplicação em pós-plantio dos herbicidas. Foram avaliados sintomas de injúria, comprimento de folha e massa seca de folha e parte aérea total. O crescimento e desenvolvimento inicial das mudas de abacaxi cultivar Smooth Cayenne não foram influenciados negativamente pela aplicação de diuron. O crescimento e desenvolvimento inicial das mudas de abacaxi cultivar Pérola não foi influenciado negativamente pela aplicação de diuron e ametryn, isolados ou associados entre si.
Assuntos
Ananas/crescimento & desenvolvimento , Ananas/parasitologia , Herbicidas/análiseRESUMO
There is a lack of information regarding the use of herbicides in pineapple crop, so the aim was to evaluate the growth and initial development of 'Smooth Cayenne' and 'Pérola' pineapple seedlings under application of diuron, sulfentrazone and ametryn, isolated and associated, in post-planting. The experiments were conducted in a greenhouse and the treatments consisted on application of herbicides after planting. Crop injury, leaf length,dry leaf mass and total aerial part were evaluated. The initial growth and development of the 'Smooth Cayenne' pineapple seedlings were not negatively influenced by the application of diuron. The initial growth and development of pineapple seedlings 'Pérola' was not negatively influenced by the application of diuron and ametryn, isolated or associated.(AU)
Há déficit de informações a respeito do uso de herbicidas no cultivo de abacaxi, assim objetivou-se avaliar o crescimento e desenvolvimento inicial de mudas de abacaxi Smooth Cayenne e Pérola sob aplicação de diuron, sulfentrazone e ametryn, isolados e em associações, em pós-plantio. Os experimentos foram conduzidos em casa de vegetação e os tratamentos foram compostos pela aplicação em pós-plantio dos herbicidas. Foram avaliados sintomas de injúria, comprimento de folha e massa seca de folha e parte aérea total. O crescimento e desenvolvimento inicial das mudas de abacaxi cultivar Smooth Cayenne não foram influenciados negativamente pela aplicação de diuron. O crescimento e desenvolvimento inicial das mudas de abacaxi cultivar Pérola não foi influenciado negativamente pela aplicação de diuron e ametryn, isolados ou associados entre si.(AU)
Assuntos
Ananas/crescimento & desenvolvimento , Ananas/parasitologia , Herbicidas/análiseRESUMO
Temporary Immersion Bioreactors (TIBs) are used for increasing plant quality and plant multiplication rates. These TIBs are actioned by mean of a pneumatic system. A failure in the pneumatic system could produce severe damages into the TIB. Consequently, the whole biological process would be aborted, increasing the production cost. Therefore, an important task is to detect failures on a temporary immersion bioreactor system. In this paper, we propose to approach this task using a contrast pattern based classifier. We show that our proposal, for detecting pneumatic failures in a TIB, outperforms other approaches reported in the literature. In addition, we introduce a feature representation based on the differences among feature values. Additionally, we collected a new pineapple micropropagation database for detecting four new types of pneumatic failures on TIBs. Finally, we provide an analysis of our experimental results together with experts in both biotechnology and pneumatic devices.
Assuntos
Reatores Biológicos , Falha de Equipamento , Reconhecimento Automatizado de Padrão/métodos , Ananas/crescimento & desenvolvimento , Área Sob a Curva , Bases de Dados como Assunto , Fatores de TempoRESUMO
Type of fertilizer and doses used are factors that influence the quality of pineapple fruit. This study aimed to evaluate the physico-chemical and sensorial quality of organically grown Pérola pineapple and recommend the effective dose for best fruit quality, especially regarding sensorial acceptance. Effect of doses of 10, 20, 30, 40 and 50 t ha-¹ of bokashi organic fertilizer on color, weight, pH, titratable acidity, soluble solids, soluble solids/titratable acidity ratio (SS/TA), vitamin C content, sensory acceptance and purchase intention was evaluated. Increasing doses of bokashi positively influenced weight and titratable acidity of the fruits and negatively affected the SS/TA ratio. Fruits produced with doses between 20t ha-¹ and 40t ha-¹. had good sensorial acceptance and were considered to have ideal acidity, sweetness and texture by most consumers. The authors recommend using 20 t ha-¹ of bokashi, as this dose promotes the production of high quality fruits, with satisfactory SS/TA ratio and high sensorial acceptance.(AU)
O tipo de adubo empregado e as doses ministradas são fatores que influenciam a qualidade do fruto de abacaxi. O estudo objetivou avaliar a qualidade físico-química e a aceitação sensorial de abacaxi Pérola orgânico e recomendar a dose de adubo que produza frutos de melhor qualidade. Avaliou-se o efeito de doses de 10, 20, 30, 40 e 50 t de fertilizante orgânico do tipo bokashi ha-¹ nas características cor, peso, acidez titulável, sólidos solúveis, ratio, vitamina C, aceitação sensorial e intenção de compra dos frutos. Doses crescentes de bokashi influenciaram positivamente a massa e a acidez titulável dos frutos e negativamente a relação sólidos solúveis/acidez titulável. Os frutos produzidos com doses entre 20t ha-¹ e 40 t ha-¹ tiveram boa aceitação sensorial e foram considerados com acidez, doçura e textura ideais pela maior parte dos consumidores. Recomenda-se o uso de 20 t de fertilizante orgânico do tipo Bokashi ha-¹, pois essa dose contribui para a produção de frutos de primeira, com relação sólidos solúveis/AT satisfatória e elevada aceitação sensorial.(AU)
Assuntos
Ananas/crescimento & desenvolvimento , Fertilizantes/análise , Frutas/efeitos dos fármacosRESUMO
Plant fiber is a renewable and biodegradable material that can be used effectively to reinforce various composites. Pineapple hybrids selected for their fiber quality are in the phase of agronomic validation in Brazil by the Embrapa Cassava and Fruits research unit. The selection of a hybrid for large-scale fiber production depends on obtaining a large number of seedlings. This study evaluated the morphogenetic response and propagation potential of eight hybrids of Ananas comosus var. erectifolius, for the purpose of producing high-quality seedlings on a large scale. Stem and crown buds were reduced and placed in MS nutritive medium supplemented with BAP at 0.5 mg L-1, NAA at 0.01 mg L-1 and Phytagel® at 2.5 g L-1. After 45 days, the number of oxidized, contaminated and surviving buds was determined. Swollen buds and plantlets were transferred to a multiplication medium containing MS sucrose, salts and vitamins. The propagation potential was evaluated based on the geometric growth rate among sub-cultures. The FIB-NEG hybrid presented the best results for the establishment phase (40.28%). The best propagative potential was obtained from crown buds with the highest values for FIB-EST (3.93), FIB-MIN (3.91) and FIB-BOY (3.91) hybrids.
A fibra vegetal é uma fonte renovável, biodegradável e de excelente desempenho como reforço em compósitos variados. Híbridos selecionados pela qualidade de suas fibras estão em fase de validação agronômica na Embrapa Mandioca e Fruticultura e sua adoção para produção de fibra em larga escala depende de um elevado número de mudas. Este trabalho teve como objetivo avaliar a resposta morfogenética e o potencial propagativo de oito híbridos de Ananas comosus var. erectifolius, com a finalidade de produzir mudas de qualidade em larga escala. Gemas do caule e coroa foram reduzidas, introduzidas em meio nutritivo MS suplementado com BAP a 0,5 mg L-1, ANA a 0,01 mg L-1 e Phytagel® a 2,5 g L-1. Aos 45 dias foram avaliados o número de gemas oxidadas, contaminadas e sobreviventes. Gemas intumescidas e plantas formadas foram transferidas para o meio de multiplicação contendo sacarose, sais e vitaminas MS. Avaliou-se o potencial propagativo a partir de uma taxa de crescimento geométrico entre subcultivos. O híbrido FIB-NEG (40.28%) apresentou os melhores resultados em porcentagem para a fase de estabelecimento. O melhor potencial propagativo foi obtido a partir de gemas de coroa, com os valores mais elevados registrados para os híbridos FIB-EST (3.93), FIB-MIN (3.91) e FIB-BOY (3.91).
Assuntos
Ananas/crescimento & desenvolvimento , Hibridização Genética , Melhoramento Vegetal/estatística & dados numéricos , Morfogênese , Modelos LinearesRESUMO
Plant fiber is a renewable and biodegradable material that can be used effectively to reinforce various composites. Pineapple hybrids selected for their fiber quality are in the phase of agronomic validation in Brazil by the Embrapa Cassava and Fruits research unit. The selection of a hybrid for large-scale fiber production depends on obtaining a large number of seedlings. This study evaluated the morphogenetic response and propagation potential of eight hybrids of Ananas comosus var. erectifolius, for the purpose of producing high-quality seedlings on a large scale. Stem and crown buds were reduced and placed in MS nutritive medium supplemented with BAP at 0.5 mg L-1, NAA at 0.01 mg L-1 and Phytagel® at 2.5 g L-1. After 45 days, the number of oxidized, contaminated and surviving buds was determined. Swollen buds and plantlets were transferred to a multiplication medium containing MS sucrose, salts and vitamins. The propagation potential was evaluated based on the geometric growth rate among sub-cultures. The FIB-NEG hybrid presented the best results for the establishment phase (40.28%). The best propagative potential was obtained from crown buds with the highest values for FIB-EST (3.93), FIB-MIN (3.91) and FIB-BOY (3.91) hybrids.(AU)
A fibra vegetal é uma fonte renovável, biodegradável e de excelente desempenho como reforço em compósitos variados. Híbridos selecionados pela qualidade de suas fibras estão em fase de validação agronômica na Embrapa Mandioca e Fruticultura e sua adoção para produção de fibra em larga escala depende de um elevado número de mudas. Este trabalho teve como objetivo avaliar a resposta morfogenética e o potencial propagativo de oito híbridos de Ananas comosus var. erectifolius, com a finalidade de produzir mudas de qualidade em larga escala. Gemas do caule e coroa foram reduzidas, introduzidas em meio nutritivo MS suplementado com BAP a 0,5 mg L-1, ANA a 0,01 mg L-1 e Phytagel® a 2,5 g L-1. Aos 45 dias foram avaliados o número de gemas oxidadas, contaminadas e sobreviventes. Gemas intumescidas e plantas formadas foram transferidas para o meio de multiplicação contendo sacarose, sais e vitaminas MS. Avaliou-se o potencial propagativo a partir de uma taxa de crescimento geométrico entre subcultivos. O híbrido FIB-NEG (40.28%) apresentou os melhores resultados em porcentagem para a fase de estabelecimento. O melhor potencial propagativo foi obtido a partir de gemas de coroa, com os valores mais elevados registrados para os híbridos FIB-EST (3.93), FIB-MIN (3.91) e FIB-BOY (3.91).(AU)
Assuntos
Ananas/crescimento & desenvolvimento , Morfogênese , Hibridização Genética , Melhoramento Vegetal/estatística & dados numéricos , Modelos LinearesRESUMO
BACKGROUND: Pineapple is the fruit of Ananas comosus var. comosus plant, being cultivated in tropical areas and has high energy content and nutritional value. Herein, 30 samples of pineapple cv. Vitória were analyzed as a function of the maturation stage (0-5) and their physico-chemical parameters monitored. In addition, negative-ion mode electrospray ionization mass spectrometry [ESI(-)FT-ICR MS] was used to identify and semi-quantify primary and secondary metabolites present in the crude and phenolic extracts of pineapple, respectively. RESULTS: Physico-chemical tests show an increase in the total soluble solids (TSS) values and in the TSS/total titratable acidity ratio as a function of the maturity stage, where a maximum value was observed in stage 3 (¾ of the fruit is yellow, which corresponds to the color of the fruit peel). ESI(-)FT-ICR MS analysis for crude extracts showed the presence mainly of sugars as primary metabolites present in deprotonated molecule form ([M - H]- and [2 M - H]- ions) whereas, for phenolic fractions, 11 compounds were detected, being the most abundant in the third stage of maturation. This behavior was confirmed by quantitative analysis of total polyphenols. CONCLUSION: ESI-FT-ICR MS was efficient in identifying primary (carbohydrates and organic acids) and secondary metabolites (13 phenolic compounds) presents in the crude and phenolic extract of the samples, respectively. © 2017 Society of Chemical Industry.
Assuntos
Ananas/crescimento & desenvolvimento , Aromatizantes/química , Frutas/química , Extratos Vegetais/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Ananas/química , Carboidratos/química , Cor , Frutas/crescimento & desenvolvimento , Polifenóis/químicaRESUMO
Asepsis, defined as the absence of microbial contamination, is one of the most important requirements of plant micropropagation. In long-term micropropagated cultures, there may occasionally occur scattered microorganism growth in the culture medium. These microorganisms are common plant components and are known as latent endophytes. Thus, the aim of this research was to investigate the presence of endophytic bacteria in asymptomatic pineapple and orchid microplants, which were cultivated in three laboratories for 1 year. Isolation and characterization of bacterial isolates, PCR-DGGE from total genomic DNA of microplants and ultrastructural analysis of leaves were performed. In the culture-dependent technique, it was only possible to obtain bacterial isolates from pineapple microplants. In this case, the bacteria genera identified in the isolation technique were Bacillus, Acinetobacter, and Methylobacterium. The scanning electron microscopy and transmission electron microscopy (SEM and TEM) analyses revealed the presence of endophytic bacteria in intracellular spaces in the leaves of pineapple and orchid microplants, independent of the laboratory or cultivation protocol. Our results strongly indicate that there are endophytic bacterial communities inhabiting the microplants before initiation of the in vitro culture and that some of these endophytes persist in their latent form and can also grow in the culture medium even after long-term micropropagation, thus discarding the concept of "truly axenic plants."
Assuntos
Ananas/microbiologia , Bactérias/isolamento & purificação , Endófitos/isolamento & purificação , Orchidaceae/microbiologia , Ananas/crescimento & desenvolvimento , Cultura Axênica , Bactérias/classificação , Bactérias/genética , Biodiversidade , DNA Bacteriano/genética , DNA Ribossômico/genética , Endófitos/classificação , Endófitos/genética , Orchidaceae/crescimento & desenvolvimento , Filogenia , RNA Ribossômico 16S/genéticaRESUMO
This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholderia silvatlantica UENF 11711, and Herbaspirillum seropedicae HRC 54), five levels of urea (0, 2, 5, 7, and 10g L-¹), and five evaluation periods (30, 60, 90, 120, and 150 days following the planting of plantlets in trays), and with four replications of each treatment. An increase in the dried weight of shoots and roots of micropropagated pineapple cv. 'Vitória' plants depended on inoculation with diazotrophic bacteria, and plantlets acclimatization could be reduced by up to 57 days, depending on the concentration of urea fertilizer.(AU)
O objetivo deste trabalho foi estudar o efeito da inoculação de bactérias diazotróficas endofíticas e adubação nitrogenada no crescimento de mudas micropropagadas de abacaxizeiro 'Vitória' durante o período de aclimatização. O delineamento experimental foi de blocos casualizados (DBC), em esquema fatorial (2x5x5), sendo os fatores representados por dois tipos de inóculos (ausência e presença de uma mistura de bactérias diazotróficas contendo Burkholderia sp. UENF 114111, Burkholderia silvatlantica UENF 117111 e Herbaspirillum seropedicae estirpe HRC 54), cinco concentrações de ureia (0, 2, 5, 7 e 10g L-¹) e em cinco épocas de avaliação do tempo de aclimatização (30, 60, 90, 120 e 150 dias após o plantio das mudas em bandejas), com quatro repetições. Verificou-se aumento na massa seca da parte aérea e massa seca da raiz de mudas micropropagadas de abacaxizeiro 'Vitória' em função da inoculação de bactérias diazotróficas. O tempo de aclimatização pode ser reduzida em até 60 dias em função da concentração de ureia.(AU)
Assuntos
Ananas/crescimento & desenvolvimento , Bactérias Fixadoras de Nitrogênio , Fertilização , Aclimatação , Fixação de Nitrogênio , Burkholderia , HerbaspirillumRESUMO
A baixa oferta de mudas sadias e com qualidade tem sido um obstáculo para o crescimento da abacaxicultura no Brasil. A pesquisa foi realizada com o objetivo de avaliar a associação micorrízica, o crescimento e absorção de nutrientes em mudas micropropagadas de abacaxizeiro Imperial inoculadas com o fungo Piriformospora indica em cultivo com a aplicação de herbicidas. Foi utilizado o esquema fatorial 2 x 4, sendo os fatores duas inoculações: mudas inoculadas com o fungo P. indica e sem inoculação e quatro doses de herbicidas: 0; 1,5; 3,0 e 6,0 L ha-1 para o ametryn, 0; 1,6; 3,2; 6,4 L ha-1 para o diuron e 0; 0,4; 0,8 e 1,6 L ha-1 para o sulfametrazone, distribuídos no delineamento inteiramente casualizado, com três repetições. As doses dos herbicidas foram aplicadas ao substrato antes do plantio das mudas. A inoculação com o fungo P. indica foi feita no momento do plantio, colocando-se no substrato discos de micélio de 5 mm, contendo estruturas fúngicas. Aos 150 dias após a inoculação foi feita a análise da colonização micorrízica, dos teores de N, P e K e do crescimento das mudas. Os dados foram submetidos à análise de variância, regressão polinomial e comparação de médias, a 5% de probabilidade. Foi observada a associação do fungo P. indica com as mudas de abacaxizeiro Imperial com a aplicação de todos os herbicidas. As mudas associadas com o fungo apresentaram maiores...(AU)
The low supply of healthy and quality nursery plants has been an obstacle to the growth of pineapple crop in Brazil. The research was carried out with the aim of evaluating mycorrhizal association, growth and nutrient uptake of Imperial micropropagated pineapple plants inoculated with the fungus Piriformospora indica in cultivation with herbicide application. The 2 x 4 factorial scheme was used, being the factors the two inoculations: inoculated nursery plants with the fungus P. indica and without inoculation and four doses of herbicides: 0, 1.5, 3.0 and 6.0 L ha-1 for ametryn, 0, 1.6, 3.2, 6.4 L ha-1 for diuron and 0, 0.4, 0.8 and 1.6 L ha-1 for sulfametrazone distributed in a completely randomized design with three replications. The doses of herbicides were applied to substrate before planting. The inoculation with the fungus P. indica was made at the time of planting, placing in the substrate 5 mm mycelial discs containing fungal structures. At 150 days after inoculation evaluations were made by analysis of colonization, determination of N, P and K levels and of nursery plants growth. Data were subjected to analysis of variance and regression. The association of fungus P. indica with Imperial pineapple nursery plants was observed with the application of all herbicides. Imperial pineapple nursery plants associated with P. indica fungus showed higher levels of nutrients...(AU)
Assuntos
Ananas/crescimento & desenvolvimento , Ananas/parasitologia , Herbicidas/administração & dosagem , MicorrizasRESUMO
A baixa oferta de mudas sadias e com qualidade tem sido um obstáculo para o crescimento da abacaxicultura no Brasil. A pesquisa foi realizada com o objetivo de avaliar a associação micorrízica, o crescimento e absorção de nutrientes em mudas micropropagadas de abacaxizeiro Imperial inoculadas com o fungo Piriformospora indica em cultivo com a aplicação de herbicidas. Foi utilizado o esquema fatorial 2 x 4, sendo os fatores duas inoculações: mudas inoculadas com o fungo P. indica e sem inoculação e quatro doses de herbicidas: 0; 1,5; 3,0 e 6,0 L ha-1 para o ametryn, 0; 1,6; 3,2; 6,4 L ha-1 para o diuron e 0; 0,4; 0,8 e 1,6 L ha-1 para o sulfametrazone, distribuídos no delineamento inteiramente casualizado, com três repetições. As doses dos herbicidas foram aplicadas ao substrato antes do plantio das mudas. A inoculação com o fungo P. indica foi feita no momento do plantio, colocando-se no substrato discos de micélio de 5 mm, contendo estruturas fúngicas. Aos 150 dias após a inoculação foi feita a análise da colonização micorrízica, dos teores de N, P e K e do crescimento das mudas. Os dados foram submetidos à análise de variância, regressão polinomial e comparação de médias, a 5% de probabilidade. Foi observada a associação do fungo P. indica com as mudas de abacaxizeiro Imperial com a aplicação de todos os herbicidas. As mudas associadas com o fungo apresentaram maiores...
The low supply of healthy and quality nursery plants has been an obstacle to the growth of pineapple crop in Brazil. The research was carried out with the aim of evaluating mycorrhizal association, growth and nutrient uptake of Imperial micropropagated pineapple plants inoculated with the fungus Piriformospora indica in cultivation with herbicide application. The 2 x 4 factorial scheme was used, being the factors the two inoculations: inoculated nursery plants with the fungus P. indica and without inoculation and four doses of herbicides: 0, 1.5, 3.0 and 6.0 L ha-1 for ametryn, 0, 1.6, 3.2, 6.4 L ha-1 for diuron and 0, 0.4, 0.8 and 1.6 L ha-1 for sulfametrazone distributed in a completely randomized design with three replications. The doses of herbicides were applied to substrate before planting. The inoculation with the fungus P. indica was made at the time of planting, placing in the substrate 5 mm mycelial discs containing fungal structures. At 150 days after inoculation evaluations were made by analysis of colonization, determination of N, P and K levels and of nursery plants growth. Data were subjected to analysis of variance and regression. The association of fungus P. indica with Imperial pineapple nursery plants was observed with the application of all herbicides. Imperial pineapple nursery plants associated with P. indica fungus showed higher levels of nutrients...
Assuntos
Ananas/crescimento & desenvolvimento , Ananas/parasitologia , Herbicidas/administração & dosagem , MicorrizasRESUMO
The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation.
Assuntos
Ananas/enzimologia , Ananas/crescimento & desenvolvimento , Bromelaínas/metabolismo , Técnicas de Cultura/métodos , Ensaios Enzimáticos/métodos , Ananas/metabolismo , Bromelaínas/biossíntese , Bromelaínas/isolamento & purificação , Meios de Cultura/química , Folhas de Planta/enzimologia , Caules de Planta/enzimologia , ProteóliseRESUMO
Many plant species grown under in vitro controlled conditions can be used as models for the study of physiological processes. Adult pineapple can display CAM physiology while in vitro it functions as a C3 plant. Ex vitro Ananas comosus has plastic morphology and physiology, both easy to modify from C3 to CAM by changing the environmental conditions. The yield of survival for a rentable propagation protocol of pineapple is closely related with the C3/CAM shift and the associated physiological characteristics. In the present work, ex vitro pineapple plants were divided in two sets and subjected to C3 and CAM-inducing environmental conditions, determined by light intensity and relative humidity, respectively, 40 µmol m(-2) s(-1)/85% and 260 µmol m(-2) s(-1)/50%. The results demonstrated that the stress imposed by the environmental conditions switched pineapple plants from C3 to CAM behavior. Comparing to CAM induced, C3-induced pineapple plants showed substandard growth parameters and morphological leaf characteristics but a better rooting process and a higher ABA production, a phenotype closer to adult plants, which are expected to produce fruits in a normal production cycle. We conclude that the upholding of these characteristics is conditioned by low light intensity plus high relative humidity, especially during the first 8 weeks of ex vitro growth. It is expected that the better understanding of pineapple acclimatization will contribute to the design of a protocol to apply as a rentable tool in the pineapple agronomic industry.
Assuntos
Aclimatação/fisiologia , Ananas/fisiologia , Carbono/metabolismo , Ácido Abscísico/análise , Ácido Abscísico/metabolismo , Irrigação Agrícola , Ananas/crescimento & desenvolvimento , Ananas/efeitos da radiação , Biomassa , Clorofila/metabolismo , Clorofila A , Meio Ambiente , Fluorescência , Umidade , Luz , Malatos/análise , Malatos/metabolismo , Estresse Oxidativo/fisiologia , Fotossíntese/fisiologia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/fisiologia , Folhas de Planta/efeitos da radiação , Temperatura , Ceras/análise , Ceras/metabolismoRESUMO
O abacaxizeiro ornamental Ananas comosus (L.) Merr. var. ananassoides (Baker) Coppens & F. Leal, também denominado de ananás do campo ou coroa verde, é uma planta ainda pouco explorada economicamente, apesar do potencial ornamental como "flor de corte". Estudos sobre a propagação do ananás do campo são escassos, neste sentido, a micropropagação desta, pode representar um avanço para a produção comercial da espécie. O objetivo do trabalho foi avaliar as diferentes combinações de 6-BAP e GA3 na multiplicação e desenvolvimento dos explantes de Ananas comosus (L.) Merr. var. ananassoides (Baker) Coppens & F. Leal. Para a condução do experimento foram utilizados brotos de abacaxizeiro ornamental pré-estabelecidos in vitro. O delineamento utilizado foi inteiramente casualizado, em esquema fatorial 4x4, constituído de quatro concentrações de 6-BAP (0; 0,25; 0,5 e 1,0 mg.L-1) e quatro concentrações GA3 (0; 0,5; 1,0 e 2,0 mg.L-1) combinadas. Os resultados obtidos indicaram que a interação 1,0 mg.L-1 de 6-BAP e 0,5 mg.L-1 de GA3 proporcionou maior número de brotações. A adição de 6-BAP propiciou uma redução do comprimento da parte aérea e do número de raízes. A adição de GA3 ocasionou um maior comprimento da parte aérea e não influenciou no enraizamento dos explantes.
The ornamental pineaple Ananas comosus (L.) Merr. var. ananassoides (Baker) Coppens & F. Leal, also denominated of pineapple of the field or it crowns green it is a plant still little explored economically in spite of the ornamental potential as " cut flower ". Studies about the propagation of the pineapple of the field are scarce, in this sense, the micropropagation of this can represent a progress for the commercial production of the species. The objective of the work was to evaluate the different combinations of 6-BAP and GA3 in the multiplication and development of the explants of Ananas comosus (L.) Merr. var. ananassoides (Baker) Coppens & F. Leal. The randomized experimental desing was used, with a factorial scheme 4x4 consisting of four concentrations of 6-BAP (0; 0,25; 0,5 and 1,0 mg.L-1) and four concentrations of GA3 (0; 0,5; 1,0 and 2,0 mg.L-1) combined. According to the results, the interaction 1,0 mg.L-1 of 6-BAP and 0,5 mg.L-1 of GA3 provided larger sprouts number. The addition of 6-BAP propitiated a reduction of the bud length and of the roots number of explants. The addition of GA3caused a larger length of the aerial part and it didn't influence in the rooting of the explants.
Assuntos
Reguladores de Crescimento de Plantas , Técnicas In Vitro , Ananas/crescimento & desenvolvimento , Desenvolvimento VegetalRESUMO
BACKGROUND: Symphylids (Hanseniella sp.) are polyphagous soilborne parasites. Today, symphylid populations on pineapple are monitored by observing root symptoms and the presence of symphylids at the bottom of basal leaves. The authors developed a reliable method with a bait and trap device to monitor symphylid populations in pineapple or fallow crops. The spatial distribution of the symphylid populations was evaluated using the variance/mean ratios and spatial analyses based on Moran's and Geary's indices. The method has been tested to monitor symphylid populations at different developmental stages of pineapple. RESULTS: Adding potato baits to the soil samples increased the trapping efficiency of symphylids when compared with 'soil only' and 'bait only' methods. The handling of the samples is also facilitated by the new device. Results showed that the vertical distribution of symphylids may be uniform deeply inside the soil profile under pineapple, up to 50 cm. Results showed that symphylid populations are highly aggregated, showing a spot area about 4-6 m wide for their development. CONCLUSION: The new method allows better and easier evaluation of symphylid populations. It may be very useful in the evaluation of new IPM methods to control symphylids under pineapple.
Assuntos
Ananas/parasitologia , Artrópodes/crescimento & desenvolvimento , Doenças das Plantas/parasitologia , Patologia Vegetal , Solo/parasitologia , Ananas/crescimento & desenvolvimento , AnimaisRESUMO
The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.