RESUMO
The health benefits of nut consumption have been extensively demonstrated in observational studies and intervention trials. Besides the high nutritional value, countless evidences show that incorporating nuts into the diet may contribute to health promotion and prevention of certain diseases. Such benefits have been mostly and certainly attributed not only to their richness in healthy lipids (plentiful in unsaturated fatty acids), but also to the presence of a vast array of phytochemicals, such as polar lipids, squalene, phytosterols, tocochromanols, and polyphenolic compounds. Thus, many nut chemical compounds apply well to the designation "nutraceuticals," a broad umbrella term used to describe any food component that, in addition to the basic nutritional value, can contribute extra health benefits. This contribution analyses the general chemical profile of groundnut and common tree nuts (almond, walnut, cashew, hazelnut, pistachio, macadamia, pecan), focusing on lipid components and phytochemicals, with a view on their bioactive properties. Relevant scientific literature linking consumption of nuts, and/or some of their components, with ameliorative and/or preventive effects on selected diseases - such as cancer, cardiovascular, metabolic, and neurodegenerative pathologies - was also reviewed. In addition, the bioactive properties were analyzed in the light of known mechanistic frameworks.
Assuntos
Suplementos Nutricionais , Juglans , Nozes , Compostos Fitoquímicos , Pistacia , Nozes/química , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/farmacologia , Humanos , Suplementos Nutricionais/análise , Juglans/química , Pistacia/química , Lipídeos/análise , Valor Nutritivo , Anacardium/química , Macadamia/química , Corylus/química , Fitosteróis/análise , Carya/química , Prunus dulcis/química , Doenças Cardiovasculares/prevenção & controleRESUMO
This study aimed to develop and validate a multi-mycotoxin analysis method applied to cashew nuts by employing a miniaturized QuEChERS method followed by determination by ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Satisfactory recoveries for the concentrations 1, 10 and 30 ng g-1, ranging from 66% (fumonisin B1) to 110% (ochratoxin A) and relative standard deviations lower than 9% (fumonisin B2) were obtained for the target compounds. Limits of quantification ranged from 0.004 ng g-1 (sterigmatocystin) to 0.59 ng g-1 (alternariol). The applicability of the analytical method was verified by analyzing 30 cashew nut samples from the city of Rio de Janeiro, RJ, southeastern Brazil. Aflatoxins M1, G2, G1, B2, B1, ochratoxin A and sterigmatocystin were detected, respectively, in 27%, 10%, 17%, 30%, 30%, 30% and 50% of the analyzed samples, at maximum concentrations of 0.56, 0.67, 1.43, 2.02, 4.93, 4.81, and 0.35 ng g-1. The maximum limit established by Brazilian legislation for aflatoxins was not exceeded by any of the analyzed samples.
Assuntos
Anacardium , Contaminação de Alimentos , Micotoxinas , Nozes , Espectrometria de Massas em Tandem , Micotoxinas/análise , Anacardium/química , Cromatografia Líquida de Alta Pressão , Contaminação de Alimentos/análise , Nozes/química , Aflatoxinas/análise , Espectrometria de Massa com Cromatografia LíquidaRESUMO
The understanding of cancer immunity and antitumor factors generated by natural polysaccharides is not yet fully comprehended. Polysaccharides, like cashew gum (CG), can exhibit immunomodulatory action and may assist in the antitumor process and side effects relieve. This study aimed to determine the antitumor effect of CG alone or in combination with cyclophosphamide (CTX), and its interactions with immune cells, in a murine melanoma model, using the B16-F10 cell line. Tumor growth inhibition, hematological, histopathological, ELISA, flow cytometry, immunofluorescence, and qRT-PCR analyses were performed to elucidate the antitumor potential, involvement of immune cells, and potential toxic effects. CG showed significant tumor growth inhibition, reaching up to 42.9 % alone and 51.4 % in combination with CTX, with mild toxicity to organs. CG enhanced leukocyte count, even in the presence of CTX. Furthermore, CG influenced the activation of tumor-associated macrophages (TAM), characterized by an increase in Il4, as well as a reduction in Ifng, Il1b, Tgfb, and Il6 gene expression. Nevertheless, these effects did not compromise the antitumor activity of CG. In summary, the combination of CG with CTX is a promising approach for leukopenia, one of the most important side effects of cancer treatment and deserves further investigation.
Assuntos
Anacardium , Ciclofosfamida , Melanoma Experimental , Animais , Ciclofosfamida/farmacologia , Camundongos , Melanoma Experimental/tratamento farmacológico , Melanoma Experimental/patologia , Anacardium/química , Gomas Vegetais/química , Gomas Vegetais/farmacologia , Linhagem Celular Tumoral , Modelos Animais de Doenças , Citocinas/metabolismo , Macrófagos Associados a Tumor/efeitos dos fármacos , Macrófagos Associados a Tumor/imunologiaRESUMO
This study evaluated the use of acerola (Malpighia glabra L., CACE), cashew (Anacardium occidentale L., CCAS), and guava (Psidium guayaba L., CGUA) fruit processing coproducts as substrates to promote the growth, metabolite production, and maintenance of the viability/metabolic activity of the probiotics Lactobacillus acidophilus LA-05 and Lacticaseibacillus paracasei L-10 during cultivation, freeze-drying, storage, and exposure to simulated gastrointestinal digestion. Probiotic lactobacilli presented high viable counts (≥8.8 log colony-forming units (CFU)/mL) and a short lag phase during 24 h of cultivation in CACE, CCAS, and CGUA. Cultivation of probiotic lactobacilli in fruit coproducts promoted sugar consumption, medium acidification, and production of organic acids over time, besides increasing the of several phenolic compounds and antioxidant activity. Probiotic lactobacilli cultivated in fruit coproducts had increased survival percentages after freeze-drying and during 120 days of refrigerated storage. Moreover, probiotic lactobacilli cultivated and freeze-dried in fruit coproducts had larger subpopulations of live and metabolically active cells when exposed to simulated gastrointestinal digestion. The results showed that fruit coproducts not only improved the growth and helped to maintain the viability and metabolic activity of probiotic strains but also enriched the final fermented products with bioactive compounds, being an innovative circular strategy for producing high-quality probiotic cultures.
Assuntos
Frutas , Probióticos , Probióticos/metabolismo , Frutas/microbiologia , Lactobacillus acidophilus/crescimento & desenvolvimento , Lactobacillus acidophilus/metabolismo , Lactobacillus acidophilus/fisiologia , Anacardium/microbiologia , Anacardium/crescimento & desenvolvimento , Psidium/crescimento & desenvolvimento , Psidium/microbiologia , Malpighiaceae/crescimento & desenvolvimento , Malpighiaceae/microbiologia , Liofilização , Viabilidade Microbiana , Lacticaseibacillus paracasei/crescimento & desenvolvimento , Lacticaseibacillus paracasei/metabolismo , Lacticaseibacillus paracasei/fisiologia , Fermentação , Manipulação de Alimentos/métodosRESUMO
The objective of the present study was to evaluate a blend of functional oils (FO) composed of copaiba and garlic essential oils, pepper oleoresin and cashew nut liquid to mitigate the effects of heat stress on productivity, egg quality, organ morphology of the gastrointestinal tract, serum biochemical profile, nutrient metabolism and body temperature of Japanese quail. A completely randomized design was used in a 3 × 2 factorial scheme (without additive; 300 and 500 mg FO/kg of feed x birds raised in a thermal comfort (TC) and heat stress (HS) environment), with 6 replicates of 8 birds per plot. The birds under HS had lower feed intake (P = 0.0000), egg mass (P = 0.0000), laying rate (P = 0.0000) and higher percentage of infertile non-commercial eggs (P = 0.0004), lower head temperature amplitude (P = 0.0000) and higher average of body temperature (P = 0.0312). HS worsened the external and internal quality of the eggs. Birds kept in HS showed higher values of cholesterol (P = 0.0000) and glutamic-pyruvic transaminase (P = 0.0272). The use of 300 and 500 g FO/ton of feed improved the feed conversion (P = 0.0000) and egg mass (P = 0.0000) of the quail bred under HS, respectively, by 8.4 and 7.2% and increased the relative weight of liver (P = 0.0064) and pancreas (P = 0.0492). The use of such additives also provided a higher % of yolk (P = 0.0018) and reduced the percentage of albumen (P = 0.0029) of the eggs produced in TC, in addition to reducing the amplitude of head temperature (P = 0.0484) of birds bred under HS. The breeding of Japanese quail under HS results in negative impacts on the production, physiological and qualitative aspects of the eggs. The use of 300 g/ton of FO in the diet of Japanese quail leads to improvements in feed conversion, increased liver capacity in nutritional metabolism and results in eggs with a higher proportion of yolks, being, therefore, an alternative for feeding quail in a hot climate.
Assuntos
Ração Animal , Coturnix , Dieta , Óleos de Plantas , Animais , Coturnix/fisiologia , Ração Animal/análise , Dieta/veterinária , Óleos de Plantas/administração & dosagem , Óleos de Plantas/farmacologia , Feminino , Distribuição Aleatória , Suplementos Nutricionais/análise , Resposta ao Choque Térmico/efeitos dos fármacos , Óleos Voláteis/administração & dosagem , Óleos Voláteis/farmacologia , Anacardium/química , Temperatura Alta/efeitos adversos , Alho/química , Transtornos de Estresse por Calor/veterináriaRESUMO
Anthracnose caused by Colletotrichum is the most severe and widely occurring cashew disease in Brazil. Colletotrichum species are commonly found as pathogens, endophytes and occasionally as saprophytes in a wide range of hosts. The endophytic species associated with cashew trees are poorly studied. In this study, we report the Colletotrichum endophytic species associated with cashew trees in two locations in the state of Pernambuco, their prevalence in different plant organs (leaves, veins, branches and inflorescences), and compare the species in terms of pathogenicity and aggressiveness using different inoculation methods (wounded × unwounded). Six species of Colletotrichum were identified according to multilocus phylogenetic analyses, including Colletotrichum asianum, Colletotrichum chrysophilum, Colletotrichum karsti, Colletotrichum siamense, Colletotrichum theobromicola, and Colletotrichum tropicale. There were differences in the percentage of isolation in relation to the prevalence of colonized tissues and collection locations. C. tropicale was the prevalent species in both geographic areas and plant tissues collected, with no pattern of distribution of species between areas and plant tissues. All isolates were pathogenic in injured tissues of cashew plants. The best method to test the pathogenicity of Colletotrichum species was utilizing the combination of leaves + presence of wounds + conidial suspension, as it better represents the natural infection process. C. siamense was the most aggressive species.
Assuntos
Anacardium , Colletotrichum , Endófitos , Filogenia , Doenças das Plantas , Colletotrichum/genética , Colletotrichum/classificação , Colletotrichum/isolamento & purificação , Brasil , Anacardium/microbiologia , Endófitos/classificação , Endófitos/genética , Endófitos/isolamento & purificação , Doenças das Plantas/microbiologia , DNA Fúngico/genética , Tipagem de Sequências MultilocusRESUMO
In this study, two nanoemulsions were formulated with essential oil (EO) of Ocimum gratissimum with (EON) or without (EOE) cashew gum (CG). Subsequently, inhibition of melanosis and preservation of the quality of shrimp stored for 16 days at 4 ± 0.5 °C were evaluated. A computational approach was performed to predict the system interactions. Dynamic light scattering (DLS) and atomic force microscopy (AFM) were used for nanoparticle analysis. Gas chromatography and flame ionization detector (GC-FID) determined the chemical composition of the EO constituents. Shrimps were evaluated according to melanosis's appearance, psychrotrophic bacteria's count, pH, total volatile basic nitrogen, and thiobarbituric acid reactive substances. EON exhibited a particle size three times smaller than EOE. The shrimp treated with EON showed a more pronounced sensory inhibition of melanosis, which was considered mild by the 16th day. Meanwhile, in the other groups, melanosis was moderate (EOE) or severe (untreated group). Both EON and EOE treatments exhibited inhibition of psychrotrophic bacteria and demonstrated the potential to prevent lipid oxidation, thus extending the shelf life compared to untreated fresh shrimp. EON with cashew gum, seems more promising due to its physicochemical characteristics and superior sensory performance in inhibiting melanosis during shrimp preservation.
Assuntos
Anacardium , Ocimum , Óleos Voláteis , Animais , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Ocimum/química , Anacardium/química , Penaeidae/química , Gomas Vegetais/química , Conservação de Alimentos/métodosRESUMO
BACKGROUND: Increased intestinal permeability and dysbiosis are related to obesity. Nuts can provide nutrients and bioactive compounds that modulate gut microbiota and inflammation, enhancing the beneficial effects of weight loss. OBJECTIVES: To evaluate the effect of consuming cashew nuts (Anacardium occidentale L.) and Brazil nuts (Bertholletia excelsa H.B.K) on intestinal permeability and microbiota, fecal SCFAs and pH, inflammation, and weight loss in energy restriction condition. METHODS: In this 8-week randomized controlled trial, 40 women with overweight or obesity were assigned to energy-restricted groups (-500 kcal/d): control group (free of nuts) or Brazilian nuts group (BN: 30 g of cashew nuts and 15 g of Brazil nuts per day). Permeability was analyzed by the lactulose/mannitol test and the microbiota by sequencing the 16S gene in the V3-V4 regions. Plasma concentrations of inflammatory cytokines (TNF, IL-6, IL-10, IL-8, IL-17A) and C-reactive protein were analyzed. RESULTS: In total, 25 women completed the intervention. Both groups lost weight without statistical differences. Lactulose excretion increased only in the control group (P < 0.05). The BN consumption increased fecal propionic acid and potentially beneficial bacteria, such as Ruminococcus, Roseburia, strains NK4A214 and UCG-002 from the Ruminococcaceae family, but also Lachnospiraceae family, Bacteroides, and Lachnoclostridium, when compared to the control group. Changes in intestinal permeability were correlated to a greater reduction in body fat (kg), and IL-8, and increases in Ruminococcus abundance. CONCLUSION: Our findings demonstrate a positive impact of BN consumption within an energy-restricted context, linked to the augmentation of potentially beneficial bacteria and pathways associated with body fat reduction. Besides, BN consumption mitigated increased intestinal permeability, although its capacity to diminish permeability or enhance weight loss proved limited. This trial was registered at the Brazilian Registry of Clinical Trials as ReBEC (ID: RBR-3ntxrm).
Assuntos
Anacardium , Bertholletia , Humanos , Feminino , Nozes/química , Anacardium/química , Sobrepeso , Brasil , Interleucina-8/análise , Lactulose , Obesidade , Inflamação , Redução de PesoRESUMO
The use of hydrogels helpsthe production of plants in drought-stress environments. Thus, this work evaluated using different hydrogels to minimize drought stress in soybean cultivation. The treatments employed two different hydrogels, one already commercialized and the other produced with cashew gum (Anacardium occidentale), five levels (0, 30, 60, 120, and 240 mg pot-1) of the hydrogels, and two levels of drought stress in sandy soil. The growth and yield of soybeans and the levels of macro- and micronutrients in soybeans were evaluated.growth. The use of CG hydrogel promoted 12% increase in protein content in the seeds in the when soybean plants were subjected to drought stress. The levels of 30 mg pot-1, corresponding to 7.5 kg ha-1, improved the 'morphological and productive parametersof the soybeans. The increasing levels of hydrogel promoted the increase in P, K, Ca, Mg, and Fe and reduced S and Cu on an exponential scale. The use of cashew gum hydrogel increased the K and Ca contents in soybean seeds compared to commercial hydrogel.
Assuntos
Anacardium , Glycine max , Secas , Hidrogéis , SoloRESUMO
There has been a continuous interest in understanding the patterns of genetic diversity in natural populations because of the role of intraspecific genetic diversity as the basis of all evolutionary change and thus, its potential effects on population persistence when facing environmental changes. Here, we provided the first description of genetic diversity distribution and population structure of Anacardium occidentale L. (cashew) from the Brazilian Cerrado, one of the most economically important tropical crops in the world. We applied Bayesian clustering approaches (STRUCTURE and POPS) that allow predicting the effects of future climatic changes on the population genetic structure of A. occidentale. We identified distinct genetic groups corresponding to the southwestern, central, and northern regions of the species' range. The characterized genetic clusters will disappear under future climate change scenarios, leading to a homogenization of genetic variability across the landscape. Our findings suggest a high likelihood for the loss of genetic diversity, which in turn will reduce the evolutionary potential of the species to cope with predicted future climatic changes. Results from this study may help develop management strategies to conserve the genetic diversity and structure of natural cashew populations.
Assuntos
Anacardium , Mudança Climática , Brasil , Teorema de Bayes , Ecossistema , Genética Populacional , Variação GenéticaRESUMO
Anthropogenic fires are an increasing threat to tropical savannas and their plant populations. In the Brazilian Cerrado, human-made fires at the end of the dry season are replacing natural fires at the beginning of the dry season. Critically, these late burns occur under more intense climate variables. Here, we aimed to understand the potential role of fire seasonality on individuals of Anacardium humile, a widespread Cerrado species of cultural and economic importance. We conducted two prescribed burnings, one at the beginning of the dry season (early burning) and one at the end of the dry season (late burning) when climate variables were remarkably different. We assessed the reproductive responses of A. humile individuals over 4 years and compared individuals from the fire treatments with those from an unburned area (control). The reproductive phenology of A. humile varied over time and was influenced by climate variables. The seasons of different burning had similar impacts on the reproductive phenology of A. humile, and this impact lasted for at least 4 years. While A. humile populations do not depend on fire for reproduction, they produced more flowers and fruits for up to 2 years with the fire treatments. We provide empirical evidence of the role of climate variables on the phenology of A. humile and demonstrate the importance of considering the role of time after fire events. The similar responses of A. humile to fire seasonality show that Cerrado fire management can be more complex than previously thought.
Assuntos
Anacardium , Ecossistema , Humanos , Pradaria , Plantas , ReproduçãoRESUMO
This study aimed to evaluate the effect of cashew nut consumption on anxiety-like behavior in dyslipidemic rats. The groups formed were: Control (CONT), Dyslipidemic (DL) and Dyslipidemic cashew nuts (DLCN). Tests to assess anxiety parameters were performed after the treatment period. Brain fatty acid profiles were analyzed. The animals in the DLCN group showed more rearing than DL, without differing from the CONT and less grooming than either the DL and CONT in the Open Field. In the Elevated Plus Maze, DLCN spent more time on the open arms and in the central area compared to the other groups. As for brain fatty acids, there was a reduction in polyunsaturated fatty acids for the DLCN compared to the other groups. The cashew nut, rich in fatty acids, phenolic and flavonoid compounds, reduced the anxiogenic-like behavior caused by dyslipidemia in rats without altering brain fatty acids.
Assuntos
Anacardium , Ansiolíticos , Dislipidemias , Animais , Ratos , Dieta Hiperlipídica/efeitos adversos , Nozes , Ácidos GraxosRESUMO
Six new ether phospholipid analogues encompassing constituents from cashew nut shell liquid as the lipid portion were synthesized in an effort to valorize byproducts of the cashew industry toward the generation of potent compounds against Chagas disease. Anacardic acids, cardanols, and cardols were used as the lipid portions and choline as the polar headgroup. The compounds were evaluated for their in vitro antiparasitic activity against different developmental stages of Trypanosoma cruzi. Compounds 16 and 17 were found to be the most potent against T. cruzi epimastigotes, trypomastigotes, and intracellular amastigotes exhibiting selectivity indices against the latter 32-fold and 7-fold higher than current drug benznidazole, respectively. Hence, four out of six analogues can be considered as hit-compounds toward the sustainable development of new treatments for Chagas disease, based on inexpensive agro-waste material.
Assuntos
Anacardium , Doença de Chagas , Tripanossomicidas , Desenvolvimento Sustentável , Nozes , Doença de Chagas/tratamento farmacológico , Doença de Chagas/parasitologia , LipídeosRESUMO
The Brazilian Northeast region has considerable agricultural potential for corn and cashew nuts production. Residues from these cultures can be densified into pellets and used as heat generators in industries and homes. In this study, corn straw pellets (CSP) and cashew nut shells pellets (CNSP) were handmade, together with a variation using glycerol as a binder (CSGP and CNSGP). All pellets were subjected to chemical, thermal and exhaust gas analyses of their combustion. All analyses were based on two different scenarios: (i) the use of CSP and CSGP for energy supply in residential use and (ii) the use of CNSP and CNSGP for energy supply in industrial use. All pellets were subjected to chemical, thermal and exhaust gas analyses of their combustion. Chemical analysis involved the study of various fuel properties, comprehending moisture content (%U), bulk density (kg m-3), volatile materials (%V), ash content (%C) and fixed carbon (%FC), and all evaluated pellets met two or more international trading standards. The combustion process analyses in the residential scenario showed higher average temperatures and lower carbon monoxide (CO) and nitrogen oxide (NOx) concentrations obtained during CSP combustion than those of CSGP, and in the industrial scenario showed average similar temperatures and lower CO and NOx concentrations obtained during CNSP combustion than those of CNSGP. Ours results demonstrate the great potential of corn straw and cashew nut shells as crops to be integrated into the biomass supply chain for energy generation and agro-ecological development.
Assuntos
Anacardium , Zea mays , Zea mays/química , Nozes/química , Temperatura , Óxidos de Nitrogênio/análise , Óxido Nítrico , BiomassaRESUMO
Nuts are important sources of antioxidants that combat oxidative stress and improve lipid profile as well as vascular function. However, the intake of typical Brazilian nuts and its acute effect on cardiovascular health needs to be better understood. Thus, the present study aimed to evaluate the acute effect of a beverage containing cashew (Anacardium occidentale L.) and Brazil nuts (Bertholletia excelsa H.B.K.) on postprandial oxidative stress, lipemia, and blood pressure of adult women aged 20 to 55 years with cardiometabolic risk. This was an acute, randomized, parallel arm, controlled clinical trial. The participants received either a beverage containing nuts (30 g Brazil nuts + 15 g cashew nuts) or a beverage without nuts with similar macronutrient composition. Oxidative stress markers and lipid profiles were evaluated at fasting and 4 h after beverage consumption. Blood pressure was measured during fasting and after beverage intake (1, 2, 3, and 4 h). In the postprandial state, there was a greater reduction in malondialdehyde levels in the intervention group compared to the control group (-12.3 ± 0.59 vs. -10.7 ± 0.43 µmol/mL; p < 0.05), which was positively correlated with the concentrations of TG (r = 0.399; p < 0.05), VLDL (r = 0.399; p < 0.05), TG/HDL (r = 0.380; p < 0.05), and blood pressure (iAUC SBP r = 0.391; p < 0.05, iAUC DBP r = 0.409; p < 0.05). The remaining oxidative stress markers showed similar postprandial changes between groups. In women with cardiometabolic risk, a beverage containing Brazilian nuts promoted a significant acute reduction on postprandial malondialdehyde levels. The study was registered in the Brazilian Clinical Trials Registry-ReBEC (protocol: RBR-3ntxrm).
Assuntos
Anacardium , Doenças Cardiovasculares , Hiperlipidemias , Adulto , Humanos , Feminino , Nozes , Pressão Sanguínea , Brasil , Estresse Oxidativo , Lipídeos , Doenças Cardiovasculares/prevenção & controle , MalondialdeídoRESUMO
Cashew tree gum is a polysaccharide material highly available in the Northeast region of Brazil. It has been explored for biocompatibility with human tissues. This research aimed to describe the synthesis and characterization of cashew gum/hydroxyapatite scaffold and evaluate the possible cytotoxicity in murine adipose-derived stem cells (ADSCs) cultures. ADSCs of the subcutaneous fat tissue of Wistar rats were collected, isolated, expanded, differentiated into three strains, and characterized immunophenotypically. The scaffolds were synthesized through chemical precipitation, lyophilized and characterized through scanning electron microscopy (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal analysis (TG and DTG), and mechanical testing. The scaffold presented a crystalline structure and pores with an average diameter of 94.45 ± 50.57 µm. By mechanical tests, the compressive force and modulus of elasticity were like the cancellous bone. The isolated adipose-derived stem cells (ADSCs) presented fibroblast morphology, adhesion capacity to plastic, differentiation in osteogenic, adipogenic and chondrogenic lineages, positive expression for the CD105 and CD90 markers and negative expression for the CD45 and CD14 markers. The MTT test showed increased cell viability, and the biomaterial showed a high level of hemocompatibility (<5 %). This study allowed the development of a new scaffold for future surgical applicability in tissue regeneration.
Assuntos
Anacardium , Materiais Biocompatíveis , Ratos , Camundongos , Humanos , Animais , Materiais Biocompatíveis/farmacologia , Alicerces Teciduais/química , Árvores , Durapatita , Ratos Wistar , Diferenciação Celular , Osteogênese , Técnicas de Cultura de Células , Engenharia Tecidual/métodosRESUMO
Insulin is one of the most important drugs in the clinical treatment of diabetes. There is growing interest in oral insulin administration as it mimics the physiological pathway and potentially reduces side effects associated with subcutaneous injection. In this study, a nanoparticulate system was developed using acetylated cashew gum (ACG) and chitosan by the polyelectrolyte complexation method, for oral administration of insulin. The nanoparticles were characterized by size, zeta potential and encapsulation efficiency (EE%). And they had a particle size of 460 ± 11.0 nm, PDI of 0.2 ± 0.021, zeta potential of 30.6 ± 0.48 mV, and an EE% of 52.5 %. Cytotoxicity assays were performed for HT-29 cell lines. It was observed that ACG and nanoparticles did not have a significant effect on cell viability, verifying their biocompatibility. Hypoglycemic effects of the formulation were analyzed in vivo, noting that the nanoparticles reduced blood glucose by 51.0 % of baseline levels after 12 h, not inducing signs of toxicity or death. Biochemical and hematological profiles were not clinically modified. Histological study indicated no signs of toxicity. Results showed that the nanostructured system presented itself as a potential vehicle for oral insulin release.
Assuntos
Anacardium , Quitosana , Diabetes Mellitus , Nanopartículas , Humanos , Insulina , Quitosana/química , Anacardium/química , Diabetes Mellitus/tratamento farmacológico , Nanopartículas/química , Portadores de Fármacos/química , Administração Oral , Tamanho da PartículaRESUMO
The industrial residue of cashew apple juice processing (MRC) was evaluated as an alternative medium for bacterial cellulose (BC) production by Komagataeibacter xylinus ATCC 53582 and Komagataeibacter xylinus ARS B42. The synthetic Hestrin-Schramm medium (MHS) was used as a control for growing and BC production. First, BC production was assessed after 4, 6, 8, 10, and 12 days under static culture. After 12 days of cultivation, K. xylinus ATCC 53582 produced the highest BC titer in MHS (3.1 g·L-1) and MRC (3 g·L-1), while significant productivity was attained at 6 days of fermentation. To understand the effect of culture medium and fermentation time on the properties of the obtained films, BC produced at 4, 6, or 8 days were submitted to infrared spectroscopy with Fourier transform, thermogravimetry, mechanical tests, water absorption capacity, scanning electron microscopy, degree of polymerization and X-ray diffraction. The properties of BC synthesized in MRC were identical to those of BC from MHS, according to structural, physical, and thermal studies. MRC, on the other hand, allows the production of BC with a high water absorption capacity when compared to MHS. Despite the lower titer (0.88 g·L-1) achieved in MRC, the BC from K. xylinus ARS B42 presented a high thermal resistance and a remarkable absorption capacity (14664 %), suggesting that it might be used as a superabsorbent biomaterial.
Assuntos
Anacardium , Gluconacetobacter xylinus , Malus , Malus/metabolismo , Celulose/química , Fermentação , Gluconacetobacter xylinus/metabolismo , Meios de Cultura/químicaRESUMO
The rising of diseases caused by multidrug-resistant bacteria has encouraged researchers to explore more antimicrobial substances, as well as chemicals capable of potentiating the action of existing ones against multidrug-resistant bacteria. Anacardium occidentale produces a fruit known as cashew nut, filled with a dark, almost black, caustic, and flammable liquid called cashew nutshell liquid (CNSL). The goal of the study was to evaluate the intrinsic antimicrobial activity of the major compounds present in CNSL, called anacardic acids (AA), as well as their possible modulatory action as an adjuvant of Norfloxacin against a Staphylococcus aureus strain overproducing the NorA efflux pump (SA1199B). Microdilution assays were performed to determine the minimum inhibitory concentration (MIC) of AA against different microbial species. Norfloxacin and Ethidium Bromide (EtBr) resistance modulation assays were performed in the presence or absence of AA against SA1199-B. AA showed antimicrobial activity against Gram-positive bacterial strains tested but no activity against Gram-negative bacteria or yeast strains. At subinhibitory concentration, AA reduced the MIC values for Norfloxacin and EtBr against the SA1199-B strain. Furthermore, AA increased the intracellular accumulation of EtBr in this NorA overproducer strain, indicating that AA are NorA inhibitors. Docking analysis showed that AA probably modulates Norfloxacin efflux by spatial impediment at the same binding site of NorA.
Assuntos
Anacardium , Infecções Estafilocócicas , Norfloxacino/farmacologia , Antibacterianos/farmacologia , Staphylococcus aureus , Anacardium/metabolismo , Ácidos Anacárdicos/farmacologia , Ácidos Anacárdicos/metabolismo , Proteínas Associadas à Resistência a Múltiplos Medicamentos , Infecções Estafilocócicas/microbiologia , Etídio/metabolismo , Etídio/farmacologia , Testes de Sensibilidade MicrobianaRESUMO
Free formaldehyde is a carcinogen whose emission reduction in particleboard has been studied recently to mitigate this environmental and human health problem. One alternative to reduce the emission of formaldehyde in particleboards is by using adhesives produced from natural sources. Cardanol-formaldehyde is an environmentally friendly adhesive made with cashew nut liquid, a byproduct from the cashew chain. This work aimed to produce particleboard using cardanol-formaldehyde in place of urea. In addition, different proportions of bean straw wastes were used to replace pine wood. The combination of eco-friendly adhesive and lignocellulosic waste particles could result in a product that meets market demands while being environmentally nonaggressive. Cardanol-formaldehyde promoted a higher modulus of elasticity (MOE) (1172 MPa) and modulus of rupture (MOR) (4.39 MPa) about panels glued with urea-formaldehyde, which presented a MOE of 764 MPa and MOR of 2.45 MPa. Furthermore, the cardanol-formaldehyde adhesive promoted a 93% reduction in formaldehyde emission, with a reduction from 16.76 to 1.09 mg/100 g oven-dry board for particleboards produced with cardanol-formaldehyde, indicating potential as an adhesive in the particleboard industry.