RESUMO
Quantitative hepatobiliary scintigraphy (Q.H.S.), with 99m Tc-DISIDA was performed on 15 control subjects and 32 alcoholic cirrhotic patients (A.C.). We used a dynamic planar scintigraphy (30 sec/ frame, up to 45 min) technique following injection intravenously of 99m TC-DISIDA. Time/activity curves were obtained from the right upper lobe of the liver and the: 1) slope uptake, 2) half-time (T 1/2 min) uptake, 3) excretion half-time (T 1/2 min), were measured from the curve. The A.C. were divided in two groups, IIA (n = 32) and IIB (n = 6) if the excretory curve show negative slope or not respectively. Results: The mean value (+/- 1 D.S. 95 percent confidence interval) of the slope uptake of the A.C. IIB (1.2 +/- 0.40) was significantly slower than a.C. IIA (2.8 +/- 0.39) and control (4.5 +/- 1.17, p = 0,0001 respectively). The difference also was significantly when the mean of A.C. IIA was compared to control (p = 0.007). The mean of T 1/2 uptake of A.C.IIB (62.2 +/- 22.2) was significantly longer than A.C. IIA (28.4 +/- 4.4 p = 0.011) and control (17.9 +/- 3.87, p = 0.003) The mean T 1/2 excretory of the A.C. IIA (90.0 +/- 17.8) was also significant delayed compared to the mean of normal control (35.6 +/- 7.6 p = 0,001). In the A.C. IIB the excretion plateau curve was associated with visualization of the gallbladder and bowel activity suggesting that the excretion of the IDA preferentially came from the left hepatic lobe. We conclude that alcoholic cirrhotic patients have impaired the mechanism related with the uptake/excretion transport of organic anion, and suggest that noninvasive Q.H.S. with 99m TC-DISIDA, can be a useful clinical technique to be used for the quantification of hepatic function in cirrhotic alcoholic patients.(AU)
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Cirrose Hepática Alcoólica/diagnóstico por imagem , Fígado/diagnóstico por imagem , Compostos de Organotecnécio , Aminoácidos/diagnóstico , Cirrose Hepática Alcoólica/fisiopatologia , Cirrose Hepática Alcoólica/metabolismo , Fígado/fisiopatologia , Fígado/metabolismo , Fatores de Tempo , Vesícula Biliar/diagnóstico por imagemRESUMO
Un péptido sintético derivado de una región conservada de la glicoproteína de transmembrana gp41 compuesto por 12 aminoácidos fue evaluado abtígeno de fase sólida en un enzimoinmunoensayo. Se analizaron 3 diferentes paneles de suero de Suecia, la Argentina y Tanzania. Se encontró una especificidad del 97.7% y 97.2% para los sueros suecos y argentinos respectivamente, siendo la sensibilidad del 100% para ambos paneles. Para el panel africano la especificidad fue del 90.5% y la sensibilidad del 96.0%. Los resultados indican que este péptido es altamente reactivo con sueros positivos para HIVl y puede ser útil en ensayos de inmunodiagnóstico (AU)