RESUMO
A segurança em laboratório se fundamenta no conhecimento das caracteristícas físico-químicas, na toxicocinética e/ou principais açöes e efeitos tóxicos das substâncias mais comumente utilizadas, como diversos solventes orgânicos, o ácido sulfúrico, a amônia e o hidróxido de sódio. É importante também, conhecer-se quais säo os riscos e os cuidados que se deve tomar no armazenamento e manuseio destes materiais e as medidas de primeiros socorros nos casos de exposiçäo excessiva.
Assuntos
Ácidos Sulfúricos/toxicidade , Amônia/toxicidade , Primeiros Socorros , Hidróxido de Sódio/toxicidade , Laboratórios , Riscos Ocupacionais , Solventes/toxicidade , Toxicologia , 1-Propanol/toxicidade , Acetona/toxicidade , Benzeno/toxicidade , Tetracloreto de Carbono/toxicidade , Clorofórmio/toxicidade , Éteres/toxicidade , Etanol/toxicidade , Metanol/toxicidade , Cloreto de Metileno/toxicidade , Tolueno/toxicidade , Xilenos/toxicidadeRESUMO
The effect of methoxyflurane anesthesia on allyl alcohol-induced hepatotoxicity and the metabolism of allyl alcohol was studied in male rats. Hepatotoxicity was assessed by the measurement of serum alanine aminotransferase activity and histopathological examination. Allyl alcohol-induced hepatotoxicity was enhanced when allyl alcohol (32 mg/kg) was administered 4 hr before or up to 8 days after a single 10-min exposure to methoxyflurane vapors. The possibility that methoxyflurane increases alcohol dehydrogenase-dependent oxidation of allyl alcohol to acrolein, the proposed toxic metabolite, was evaluated by measuring the rate of acrolein formation in the presence of allyl alcohol and liver cytosol. The effect of methoxyflurane on alcohol dehydrogenase activity in liver cytosol was also assessed by measuring the rate of NAD+ utilization in the presence of ethyl alcohol or allyl alcohol. Alcohol dehydrogenase activity and rate of acrolein formation were elevated in methoxyflurane-pretreated rats. The results suggest that a modest increase in alcohol dehydrogenase activity and rate of acrolein formation markedly enhances allyl alcohol-induced hepatotoxicity.