RESUMO
In dogs, Rhipicephalus linnaei transmits pathogens such as Ehrlichia canis, Babesia vogeli, and Hepatozoon canis. The veterinary market has synthetic acaricides to ticks control. Esters derived from castor oil are efficient. However, there is little information about their effects on non-target organisms. This work consisted of a clinical (AST, ALT, and ALP) and histological and histochemical analysis (liver and spleen) of female rabbits exposed to these esters and afoxolaner. The rabbits were divided into three groups: control group (CG) received Bandeirante® rabbit feed; the afoxolaner treatment (TG1) received rabbit feed and two doses of afoxolaner; castor oil esters treatment (TG2) received rabbit feed enriched with esters (1.75 g esters/kg). No alterations were observed in the AST, ALT, and ALP enzymes in exposure to esters TG2. Rabbits from TG1 showed changes in AST. The liver of rabbits exposed to afoxolaner underwent histological and histochemical changes, such as steatosis and vacuolation, as well as poor protein labeling. Polysaccharides were intensely observed in the group exposed to esters. The spleen showed no changes in any of the exposure. Esters from castor oil caused fewer liver changes when incorporated into the feed and fed to rabbits than exposure to afoxolaner.
Assuntos
Acaricidas , Doenças do Cão , Rhipicephalus sanguineus , Rhipicephalus , Feminino , Coelhos , Animais , Cães , Óleo de Rícino/química , Óleo de Rícino/farmacologia , Óleo de Rícino/uso terapêutico , Acaricidas/farmacologia , Baço , Ésteres/farmacologia , Ésteres/uso terapêutico , Fígado , Doenças do Cão/parasitologiaRESUMO
OBJECTIVE: The main purpose of this study was to evaluate the characteristics of a vegetal polymeric biomaterial intended for bone substitution in horses and to investigate the responses of the equine third metacarpal bone to biomaterial implantation. MATERIALS AND METHODS: Six horses were submitted to osteotomy on the dorsal aspect of the left and right third metacarpal bones; one bone defect was randomly selected for treatment with biopolymer, while the other was left untreated and served as a control. Bone density was monitored radiographically after surgery and bone biopsy fragments were collected at the end of the 120-day follow-up period. Biopsy fragments were analysed using light and scanning electron microscopy. RESULTS: Mean bone density values (mmAL) were greater in control defects (16.33 ± 1.6) than in polymer-treated defects (14.17 ± 1.7) at 120 days (p = 0.027). Light microscopy revealed greater percentages of new bone formation in control defects (50.15 ± 14.8) than in polymer treated defects (26.94 ± 12.1) at 120 days (p < 0.0001). Scanning electron microscopy analysis suggested a similar quality of pre-existing bone and new bone formed in the presence of biomaterial. CLINICAL SIGNIFICANCE: The absence of adverse reactions supports biomaterial biocompatibility and osteoconducting capacity and suggests the castor oil polymer is a suitable bone substitute for the treatment of bone defects in horses.
Assuntos
Substitutos Ósseos/uso terapêutico , Óleo de Rícino/uso terapêutico , Ossos Metacarpais/cirurgia , Animais , Materiais Biocompatíveis/uso terapêutico , Densidade Óssea , Regeneração Óssea/efeitos dos fármacos , Cavalos/cirurgia , Masculino , Ossos Metacarpais/diagnóstico por imagem , Ossos Metacarpais/ultraestrutura , Microscopia Eletrônica de Varredura/veterinária , Osteotomia/veterinária , Polímeros/uso terapêutico , RadiografiaRESUMO
STATEMENT OF PROBLEM: The prevalence of complete edentulism remains high in the elderly, and previous data have shown that poor denture hygiene is common among patients with edentulism. PURPOSE: The purpose of this randomized crossover trial was to evaluate the efficacy of denture cleansers in terms of biofilm removal, antimicrobial action, and the remission of denture stomatitis. MATERIAL AND METHODS: Fifty denture wearers with denture stomatitis were instructed to brush their dentures (brush and soap) and to soak them (20 minutes/14 days) in 4 solutions, as follows: C (control), 0.85% saline; SH1, 0.1% sodium hypochlorite; SH2, 0.2% sodium hypochlorite; and RC, 8% Ricinus communis. The biofilm in the intaglio surface of maxillary dentures was stained, photographed, and quantified by software (Image Tool). It was then collected (brushed with saline solution), and the obtained suspension was diluted (100 to 10-3) and seeded (50 µL) in CHROMagar for Candida spp. After incubation, colony-forming units per milliliter values were calculated. Denture stomatitis remission was classified according to the Newton classification. Data were analyzed by Friedman (α=.05) and Wilcoxon tests and corrected by the Bonferroni test (α=.005). RESULTS: SH1 (mean rank [MR]=1.98) and SH2 (MR=1.64) showed lower biofilm coverage than C (MR=3.73) that was similar to RC (MR=2.92). SH1 (MR=2.43) and SH2 (MR=2.10) showed antimicrobial action for Candida spp, and RC (MR=3.36) showed similar results to C (MR=3.51) and baseline (MR=3.50). Clinical signs of denture stomatitis were reduced by SH1 (MR=2.44), while SH2 (MR=2.56) and RC (MR=2.74) showed intermediate results. CONCLUSIONS: The two sodium hypochlorite solutions were the most effective means of biofilm control. All tested solutions were effective in reducing the signs of denture stomatitis.
Assuntos
Biofilmes/efeitos dos fármacos , Óleo de Rícino/uso terapêutico , Higienizadores de Dentadura/uso terapêutico , Desinfetantes/uso terapêutico , Ricinus , Hipoclorito de Sódio/uso terapêutico , Idoso , Idoso de 80 Anos ou mais , Estudos Cross-Over , Dentaduras/microbiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estomatite sob Prótese/prevenção & controle , Resultado do TratamentoRESUMO
Background: The use of antiseptics to inhibit or destroy microorganisms through synthetic or natural substances helps speed the healing process. Herbal derivatives of ricinoleic acid extracted from castor beans (Ricinus communis), is an important ally in the treatment of wounds of various animal species during the different stages of the healing process. This study investigates the use of a castor oil based ointment as an antiseptic and wound healing agent to treat an extensive lacerating wound in a senile horse. Case: A 17-year-old Quarter Horse presenting laceration located in the region of the semitendinosus and semimembranosus muscles of the right hindlimb. No changes were observed upon clinical examination while the haematological parameters creatine kinase and fibrinogen increased due to changes. The lesion initially was 35 cm long, and 19 cm wide and second intention healing was the treatment of choice after evaluation. The experimental use of the phytotherapic based on castor oil (Ricinus Assept®) was suggested. This choice was based on the horse owner request for a low-cost treatment. Following the decision, the treatment started with injury debridement by removing devitalized tissue followed by application of castor oil. This treatment was performed twice a day during 24 weeks, with curettage every 7 days to determine the progress of the healing process. The monitori...(AU)
Assuntos
Animais , Óleo de Rícino/uso terapêutico , Ricinus , Ferimentos e Lesões/terapia , Cavalos , Plantas Medicinais/químicaRESUMO
Background: The use of antiseptics to inhibit or destroy microorganisms through synthetic or natural substances helps speed the healing process. Herbal derivatives of ricinoleic acid extracted from castor beans (Ricinus communis), is an important ally in the treatment of wounds of various animal species during the different stages of the healing process. This study investigates the use of a castor oil based ointment as an antiseptic and wound healing agent to treat an extensive lacerating wound in a senile horse. Case: A 17-year-old Quarter Horse presenting laceration located in the region of the semitendinosus and semimembranosus muscles of the right hindlimb. No changes were observed upon clinical examination while the haematological parameters creatine kinase and fibrinogen increased due to changes. The lesion initially was 35 cm long, and 19 cm wide and second intention healing was the treatment of choice after evaluation. The experimental use of the phytotherapic based on castor oil (Ricinus Assept®) was suggested. This choice was based on the horse owner request for a low-cost treatment. Following the decision, the treatment started with injury debridement by removing devitalized tissue followed by application of castor oil. This treatment was performed twice a day during 24 weeks, with curettage every 7 days to determine the progress of the healing process. The monitori...
Assuntos
Animais , Cavalos , Ferimentos e Lesões/terapia , Ricinus , Óleo de Rícino/uso terapêutico , Plantas Medicinais/químicaRESUMO
PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
Assuntos
Matriz Óssea , Substitutos Ósseos/uso terapêutico , Óleo de Rícino/uso terapêutico , Durapatita/uso terapêutico , Células-Tronco Mesenquimais/efeitos dos fármacos , Poliuretanos/uso terapêutico , Alicerces Teciduais , Animais , Regeneração Óssea/efeitos dos fármacos , Adesão Celular , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Cães , Teste de Materiais , Células-Tronco Mesenquimais/citologia , Microscopia Eletrônica de Varredura , Osteogênese/efeitos dos fármacos , Propriedades de Superfície , Engenharia TecidualRESUMO
PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
Assuntos
Animais , Cães , Matriz Óssea , Substitutos Ósseos/uso terapêutico , Óleo de Rícino/uso terapêutico , Durapatita/uso terapêutico , Células-Tronco Mesenquimais/efeitos dos fármacos , Poliuretanos/uso terapêutico , Alicerces Teciduais , Regeneração Óssea/efeitos dos fármacos , Adesão Celular , Células Cultivadas , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Teste de Materiais , Microscopia Eletrônica de Varredura , Células-Tronco Mesenquimais/citologia , Osteogênese/efeitos dos fármacos , Propriedades de Superfície , Engenharia TecidualRESUMO
Root perforations may lead to a loss of integrity in the root and periodontium, violations of the biologic periodontal distance, and injuries to periodontal tissue. This study sought to analyze the effect of root canal biomechanical preparation on the microhardness and the marginal sealing ability of different materials used to treat root perforations. Standard root perforations were performed in 96 bovine incisors. The teeth were divided into four groups (n = 24), based on the material used to treat those teeth: Mineral trioxide aggregate (MTA) (Group 1), MTA protected with cyanoacrylate (Group 2), MTA protected with glass ionomer (GI) cement (Group 3), and castor oil bean (COB) cement (Group 4). After root perforations were closed, the root canals were prepared biomechanically and teeth were sectioned longitudinally. Microleakage and microhardness of sealed perforations were assessed; microleakage data were submitted to analysis of variance (ANOVA) testing, while microhardness data were submitted to Dunnet and Tukey tests (p < 0.05). Group 4 reported the lowest amount of microleakage (0.65 mm), followed by Group 3 (1.02 mm), Group 1 (1.14 mm), and Group 2 (1.30 mm); however, no difference was detected among the groups. Groups 1-3 demonstrated significantly higher microhardness values compared to COB. It was concluded that the chemical and mechanical agents used during root canal preparation did not affect the sealing procedures. Administering surface protection to MTA did not improve microhardness or sealing.
Assuntos
Colagem Dentária , Cavidade Pulpar/lesões , Materiais Restauradores do Canal Radicular/química , Compostos de Alumínio/química , Compostos de Alumínio/uso terapêutico , Animais , Fenômenos Biomecânicos , Compostos de Cálcio/química , Compostos de Cálcio/uso terapêutico , Óleo de Rícino/química , Óleo de Rícino/uso terapêutico , Bovinos , Cianoacrilatos/química , Cianoacrilatos/uso terapêutico , Cimentos Dentários/química , Cimentos Dentários/uso terapêutico , Infiltração Dentária/classificação , Combinação de Medicamentos , Corantes Fluorescentes , Cimentos de Ionômeros de Vidro/química , Cimentos de Ionômeros de Vidro/uso terapêutico , Dureza , Umidade , Teste de Materiais , Óxidos/química , Óxidos/uso terapêutico , Rodaminas , Materiais Restauradores do Canal Radicular/uso terapêutico , Preparo de Canal Radicular/métodos , Silicatos/química , Silicatos/uso terapêutico , Propriedades de Superfície , Temperatura , Fatores de TempoRESUMO
OBJETIVO: Determinar se a reação tecidual do implante retroperitoneal do polímero de óleo de mamona (Pm) é significativa ou não por meio de análise histopatológica, tendo como controle o implante de titânio (Ti). MÉTODOS: Estudo experimental, intervencionista e randomizado com 32 cobaias. Os animais foram divididos em quatro grupos iguais e eutanasiados com 7, 20, 30 e 40 dias após o ato cirúrgico. Foram confeccionadas lâminas em hematoxilina-eosina e em tricrômio de Masson. Na comparação dos tipos de material em relação a variáveis quantitativas, foi considerado o teste não-paramétrico de Wilcoxon. Em relação a essas variáveis, os grupos definidos pelo dia do sacrifício foram comparados usando-se o teste não-paramétrico de Kruskal-Wallis. Os resultados obtidos no estudo da fibrose foram expressos por frequências e percentuais. Para a comparação entre os grupos definidos pelo dia do sacrifício, em relação a variáveis qualitativas dicotômicas da fibrose, foi considerado o teste exato de Fisher. Para avaliar a diferença entre os materiais titânio e polímero em cada grupo, ainda em se tratando da fibrose, foi considerado o teste binomial. Valores de P<0,05 indicaram significância estatística. RESULTADOS: A análise das variáveis quantitativas não demonstrou diferença nas reações teciduais entre os materiais (P>0,05). A análise da variável qualitativa também não demonstrou diferença entre as reações teciduais dos materiais (P>0,05). CONCLUSÃO: Não foi encontrada significância estatística entre a reação tecidual do Pm e do Ti.
OBJECTIVE: The aim of the present investigation is to determine if the tissue reaction to the Riccinus communis (mamona) polymer has significant statistical difference compared to the tissue reaction provoked by the titanium implant. METHODS: Thirty two Cavia porcellus were divided into four groups containing eight animals each one. We implanted the two types of materials in the retroperitoneal space of all the animals. They were sacrificed at 7, 20, 30 and 40 days after surgery and the samples were submitted to histological study. RESULTS: The quantitative analysis did not show difference between the tissue reaction of the two materials (P>0.05). The analysis of the qualitative variable also did not show difference between the tissue reaction of the materials (P>0.05). CONCLUSION: Macroscopic and microscopic results showed that the castor oil polymer implant has no significant statistical difference compared to the titanium implant tissue reaction.
Assuntos
Animais , Cobaias , Masculino , Materiais Biocompatíveis/uso terapêutico , Óleo de Rícino/uso terapêutico , Coração Artificial , Polímeros/uso terapêutico , Ricinus , Titânio/uso terapêutico , Fibrose/patologia , Células Gigantes , Histiócitos , Implantes Experimentais , Linfócitos , Teste de Materiais , Modelos Animais , Neutrófilos , Distribuição Aleatória , Reprodutibilidade dos Testes , Resultado do TratamentoRESUMO
PURPOSE: To evaluate the biologic behavior of the castor polymer containing silica nanoparticles as a bone substitute in diafisary defect. METHODS: Twenty seven male rattus norvegicus albinus wistar lineage were submitted to bone defect filled with castor oil polymer. Three experimental groups had been formed with nine animals each: (1) castor oil polymer containing only calcium carbonate; (2) castor oil polymer with calcium carbonate and doped with 5 percent of silica nanoparticles; (3) castor polymer with calcium carbonate doped with 10 percent of silica nanoparticles; 3 animals of each group were submitted to euthanasia 15, 30 and 60 days after experimental procedure, and their femurs were removed to histological evaluation. RESULTS: there was bone growth in all the studied groups, with a greater tendency of growth in the group 1. After 30 days all the groups presented similar results. After 60 days a greater amount of fibroblasts, osteoblasts, osteocytes and osteoclasts in group 3 was observed, with integrated activity of 3 kinds of cells involved in the bone activation-reabsorption-formation. CONCLUSIONS: The castor polymer associated to the silica nanoparticles is biocompatible and allows osteoconduction. The presence of osteoprogenitors cells suggests silica osteoinduction capacity.
OBJETIVO: Avaliar o comportamento biológico do polímero de mamona contendo nanopartículas de sílica como substituto ósseo. MÉTODOS: Vinte e sete rattus norvergicus albinus, Wistar foram submetidos a defeito ósseo preenchido com polímero de mamona. Foram formados três grupos experimentais, com nove ratos cada: (1) Polímero com carbonato de cálcio; (2) Polímero com carbonato de cálcio dopado com 5 por cento de nanopartículas de sílica; (3) Polímero com carbonato de cálcio dopado com 10 por cento de nanopartículas de sílica; três animais de cada grupo foram submetidos à eutanásia 15, 30 e 60 dias após o procedimento experimental e os fêmures removidos e submetidos à avaliação histológica. RESULTADOS: Houve crescimento ósseo em todos os grupos estudados, com maior tendência de crescimento no grupo contendo polímero de mamona acrescido apenas por carbonato de cálcio. Aos 30 dias, todos os grupos apresentaram resultados semelhantes. Aos 60 dias, notou-se maior presença de fibroblastos, osteoblastos, osteócitos e osteoclastos no grupo 3, com persistência da atividade integrada dos três tipos de células envolvidas no processo de ativação-reabsorção-formação óssea. CONCLUSÕES: O polímero de mamona associado com nanopartículas de sílica é biocompatível e permite a osteocondução. A presença de células osteoprogenitoras nos implantes contendo 10 por cento de sílica indica sua capacidade osteoindutora.
Assuntos
Animais , Masculino , Ratos , Regeneração Óssea/efeitos dos fármacos , Substitutos Ósseos/uso terapêutico , Óleo de Rícino/uso terapêutico , Nanopartículas/química , Poliuretanos/química , Dióxido de Silício/química , Regeneração Óssea/fisiologia , Óleo de Rícino/química , Teste de Materiais , Osteoblastos/efeitos dos fármacos , Ratos WistarRESUMO
PURPOSE: To evaluate the biologic behavior of the castor polymer containing silica nanoparticles as a bone substitute in diafisary defect. METHODS: Twenty seven male Rattus norvegicus albinus Wistar lineage were submitted to bone defect filled with castor oil polymer. Three experimental groups had been formed with nine animals each: (1) castor oil polymer containing only calcium carbonate; (2) castor oil polymer with calcium carbonate and doped with 5% of silica nanoparticles; (3) castor polymer with calcium carbonate doped with 10% of silica nanoparticles; 3 animals of each group were submitted to euthanasia 15, 30 and 60 days after experimental procedure, and their femurs were removed to histological evaluation. RESULTS: there was bone growth in all the studied groups, with a greater tendency of growth in the group 1. After 30 days all the groups presented similar results. After 60 days a greater amount of fibroblasts, osteoblasts, osteocytes and osteoclasts in group 3 was observed, with integrated activity of 3 kinds of cells involved in the bone activation-reabsorption-formation. CONCLUSIONS: The castor polymer associated to the silica nanoparticles is biocompatible and allows osteoconduction. The presence of osteoprogenitors cells suggests silica osteoinduction capacity.
Assuntos
Regeneração Óssea/efeitos dos fármacos , Substitutos Ósseos/uso terapêutico , Óleo de Rícino/uso terapêutico , Nanopartículas/química , Poliuretanos/química , Dióxido de Silício/química , Animais , Regeneração Óssea/fisiologia , Óleo de Rícino/química , Masculino , Teste de Materiais , Osteoblastos/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
OBJECTIVE: The aim of the present investigation is to determine if the tissue reaction to the Riccinus communis (mamona) polymer has significant statistical difference compared to the tissue reaction provoked by the titanium implant. METHODS: Thirty two Cavia porcellus were divided into four groups containing eight animals each one. We implanted the two types of materials in the retroperitoneal space of all the animals. They were sacrificed at 7, 20, 30 and 40 days after surgery and the samples were submitted to histological study. RESULTS: The quantitative analysis did not show difference between the tissue reaction of the two materials (P>0.05). The analysis of the qualitative variable also did not show difference between the tissue reaction of the materials (P>0.05). CONCLUSION: Macroscopic and microscopic results showed that the castor oil polymer implant has no significant statistical difference compared to the titanium implant tissue reaction.
Assuntos
Materiais Biocompatíveis/uso terapêutico , Óleo de Rícino/uso terapêutico , Coração Artificial , Polímeros/uso terapêutico , Ricinus , Titânio/uso terapêutico , Animais , Fibrose/patologia , Células Gigantes , Cobaias , Histiócitos , Implantes Experimentais , Linfócitos , Masculino , Teste de Materiais , Modelos Animais , Neutrófilos , Distribuição Aleatória , Reprodutibilidade dos Testes , Resultado do TratamentoRESUMO
UNLABELLED: Bone tissue lesions can be caused by congenital and acquired factors, and result in nasal deformities with cosmetic and functional repercussion. Surgical treatment in these cases frequently requires complex reconstructions and the use of biomaterials. The polyurethane derived from castor beans (Ricinus communis) has a favorable formulation in terms of ease of processing, flexibility, no emission of toxic vapors and low cost. Nonetheless, despite favorable results, studies about the use of castor beam polymer (Ricinus communis) assessing tissue reaction on the nasal dorsum are still missing in the literature. AIM: the goal of the present investigation is to histologically assess the Ricinus communis polymer implant biocompatibility with the nasal dorsum. STUDY DESIGN: experimental. MATERIALS AND METHODS: we used four Cebus appela monkeys, in which we created a nasal dorsal defect in all the animals and there we placed the aforementioned implant. The animals were sacrificed 270 days after surgery and the samples were submitted to histological study. RESULTS: in the histology analysis we did not observe the presence of foreign body granulomas or phagocytic cells. We also observed a progressive bone formation and maturation. CONCLUSION: macroscopic and microscopic results showed that the castor oil polymer implant was biocompatible.
Assuntos
Materiais Biocompatíveis/uso terapêutico , Substitutos Ósseos/uso terapêutico , Óleo de Rícino/uso terapêutico , Osso Nasal/cirurgia , Ricinus communis/química , Animais , Cebus , Teste de Materiais/métodos , Poliuretanos , Fatores de TempoRESUMO
Bone tissue lesions can be caused by congenital and acquired factors, and result in nasal deformities with cosmetic and functional repercussion. Surgical treatment in these cases frequently requires complex reconstructions and the use of biomaterials. The polyurethane derived from castor beans (Ricinus communis) has a favorable formulation in terms of ease of processing, flexibility, no emission of toxic vapors and low cost. Nonetheless, despite favorable results, studies about the use of castor beam polymer (Ricinus communis) assessing tissue reaction on the nasal dorsum are still missing in the literature. AIM: the goal of the present investigation is to histologically assess the Ricinus communis polymer implant biocompatibility with the nasal dorsum. STUDY DESING: experimental. MATERIALS AND METHODS: we used four Cebus appela monkeys, in which we created a nasal dorsal defect in all the animals and there we placed the aforementioned implant. The animals were sacrificed 270 days after surgery and the samples were submitted to histological study. RESULTS: in the histology analysis we did not observe the presence of foreign body granulomas or phagocytic cells. We also observed a progressive bone formation and maturation. CONCLUSION: macroscopic and microscopic results showed that the castor oil polymer implant was biocompatible.
Lesões do tecido ósseo podem ser causadas por fatores congênitos e adquiridos e resultar em deformidade nasal com repercussão estética e funcional. O tratamento cirúrgico desses casos requer reconstruções complexas e frequentemente o uso de biomateriais. O poliuretano derivado do óleo da mamona apresenta uma fórmula com aspectos favoráveis de processabilidade, flexibilidade de formulação, ausência de emissão de vapores tóxicos e baixo custo. Entretanto, a despeito dos resultados favoráveis, estudos referentes ao uso do polímero de mamona, avaliando a reação tecidual no dorso nasal, ainda não foram realizados. OBJETIVO: O objetivo deste estudo consiste em avaliar histologicamente a biocompatibilidade do implante do polímero de mamona no dorso nasal. FORMA DE ESTUDO: Experimental. MATERIAL E MÉTODO: Foram utilizados quatro macacos-pregos da espécie Cebus apella. Um defeito ósseo foi realizado no osso nasal em todos os animais e colocado um implante de polímero de mamona. A eutanásia foi realizada com 270 dias de pós-operatório, e as amostras foram submetidas a estudo histológico. RESULTADOS: Na análise histológica não foi observada a presença de granuloma de corpo estranho ou células fagocitárias. Progressiva formação óssea e maturação foram observadas. CONCLUSÃO: Os resultados macroscópicos e microscópicos mostraram que o implante de polímero de mamona foi biocompatível.
Assuntos
Animais , Materiais Biocompatíveis/uso terapêutico , Substitutos Ósseos/uso terapêutico , Ricinus communis/química , Óleo de Rícino/uso terapêutico , Osso Nasal/cirurgia , Cebus , Teste de Materiais/métodos , Poliuretanos , Fatores de TempoRESUMO
Innocuous biocompatible materials have been searched to repair or reconstruct bone defects. Their goal is to restore the function of live or dead tissues. This study compared connective tissue and bone reaction when exposed to demineralized bovine bone matrix and a polyurethane resin derived from castor bean (Ricinus communis). Forty-five rats were assigned to 3 groups of 15 animals (control, bovine bone and polyurethane). A cylindrical defect was created on mandible base and filled with bovine bone matrix and the polyurethane. Control group received no treatment. Analyses were performed after 15, 45 and 60 days (5 animals each). Histological analysis revealed connective tissue tolerance to bovine bone with local inflammatory response similar to that of the control group. After 15 days, all groups demonstrated similar outcomes, with mild inflammatory reaction, probably due to the surgical procedure rather than to the material. In the polymer group, after 60 days, scarce multinucleated cells could still be observed. In general, all groups showed good stability and osteogenic connective tissue with blood vessels into the surgical area. The results suggest biocompatibility of both materials, seen by their integration into rat mandible. Moreover, the polyurethane seems to be an alternative in bone reconstruction and it is an inexhaustible source of biomaterial.
Assuntos
Materiais Biocompatíveis/uso terapêutico , Matriz Óssea/transplante , Regeneração Óssea/fisiologia , Substitutos Ósseos/uso terapêutico , Óleo de Rícino/uso terapêutico , Poliuretanos/uso terapêutico , Animais , Matriz Óssea/patologia , Bovinos , Colágeno , Tecido Conjuntivo/irrigação sanguínea , Tecido Conjuntivo/patologia , Masculino , Mandíbula/patologia , Doenças Mandibulares/patologia , Doenças Mandibulares/cirurgia , Osteoblastos/patologia , Osteoclastos/patologia , Osteócitos/patologia , Osteogênese/fisiologia , Ratos , Ratos Sprague-Dawley , Fatores de Tempo , Transplante HeterólogoRESUMO
OBJECTIVE: The goal of this study was to check whether leakage results of the same specimens measured by 2 different leakage models are similar. STUDY DESIGN: Canine root canals were prepared and filled with cold gutta-percha cones and 1 of 4 sealers (20 canals for each sealer). The 80 specimens were first connected to a fluid transport model where air-bubble movement was measured. The same specimens were later connected to a glucose penetration model where the concentration of glucose was measured. In both models, a headspace pressure of 30 kPa was used to accelerate leakage. RESULTS: In both models, 4 sealers ranked the same regarding the leakage they allowed, and a significant correlation between the results of the 2 models was confined (Spearman test coefficient = 0.65; P = .000001). CONCLUSION: Under the conditions of this study, leakage results of 80 specimens recorded in the fluid transport model and glucose penetration model were similar.
Assuntos
Colagem Dentária/métodos , Infiltração Dentária/classificação , Teste de Materiais/métodos , Materiais Restauradores do Canal Radicular/uso terapêutico , Ar , Bismuto/uso terapêutico , Óleo de Rícino/uso terapêutico , Dente Canino/anatomia & histologia , Cimentos Dentários/uso terapêutico , Cavidade Pulpar/anatomia & histologia , Combinação de Medicamentos , Resinas Epóxi/uso terapêutico , Glucose , Guta-Percha/uso terapêutico , Humanos , Pressão , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular/instrumentação , Prata/uso terapêutico , Hipoclorito de Sódio/uso terapêutico , Espectrofotometria , Fatores de Tempo , Titânio/uso terapêuticoRESUMO
Innocuous biocompatible materials have been searched to repair or reconstruct bone defects. Their goal is to restore the function of live or dead tissues. This study compared connective tissue and bone reaction when exposed to demineralized bovine bone matrix and a polyurethane resin derived from castor bean (Ricinus communis). Forty-five rats were assigned to 3 groups of 15 animals (control, bovine bone and polyurethane). A cylindrical defect was created on mandible base and filled with bovine bone matrix and the polyurethane. Control group received no treatment. Analyses were performed after 15, 45 and 60 days (5 animals each). Histological analysis revealed connective tissue tolerance to bovine bone with local inflammatory response similar to that of the control group. After 15 days, all groups demonstrated similar outcomes, with mild inflammatory reaction, probably due to the surgical procedure rather than to the material. In the polymer group, after 60 days, scarce multinucleated cells could still be observed. In general, all groups showed good stability and osteogenic connective tissue with blood vessels into the surgical area. The results suggest biocompatibility of both materials, seen by their integration into rat mandible. Moreover, the polyurethane seems to be an alternative in bone reconstruction and it is an inexhaustible source of biomaterial.
Assuntos
Animais , Bovinos , Masculino , Ratos , Materiais Biocompatíveis/uso terapêutico , Matriz Óssea/transplante , Regeneração Óssea/fisiologia , Substitutos Ósseos/uso terapêutico , Óleo de Rícino/uso terapêutico , Poliuretanos/uso terapêutico , Matriz Óssea/patologia , Colágeno , Tecido Conjuntivo/irrigação sanguínea , Tecido Conjuntivo/patologia , Mandíbula/patologia , Doenças Mandibulares/patologia , Doenças Mandibulares/cirurgia , Osteoblastos/patologia , Osteoclastos/patologia , Osteócitos/patologia , Osteogênese/fisiologia , Ratos Sprague-Dawley , Fatores de Tempo , Transplante HeterólogoRESUMO
The aim of this study is to compare polyurethanes containing castor oil (soft segment) in granular form compared to cancellous bone autograft applied to a segmental bone defect. Norfolk adult female rabbits - approximately 13 months of age with a mean body weight of 4.5 kg - are used. In both radial diaphyses, 1 cm osteoperiosteal segmental defects are created. The defect in the left radius is filled with the castor-oil-based polyurethane, and the right one, filled with cancellous bone autograft, collected from the left proximal humerus. The rabbits are euthanazed at 15, 30, 60, and 120 days postsurgery (5 animals/ period), for histological analyses. By radiographic analyses, at these time points, the bone regeneration is more evident and accelerated in the bone defects treated with the cancellous bone autograft. At 120 days postsurgery, the segmental bone defects treated with the cancellous bone autograft are totally reconstituted and remodeled, while the bone defects treated with polyurethane polymer have bone formation of 79%. Histological study shows that the polyurethane acts as a space filler, minimizing the local production of fibrous tissue. No granule degradation, resorption or any inflammatory reaction is detected. Thus, it is possible to conclude that the castor-oil-plant-based polyurethane - in the granule presentation - is biocompatible and osteointegrated, but does not show the same bone regeneration capacity as the cancellous bone autograft.
Assuntos
Substitutos Ósseos/administração & dosagem , Óleo de Rícino/uso terapêutico , Consolidação da Fratura/fisiologia , Úmero/transplante , Poliuretanos/uso terapêutico , Fraturas do Rádio/diagnóstico por imagem , Fraturas do Rádio/terapia , Animais , Substitutos Ósseos/química , Óleo de Rícino/química , Feminino , Poliuretanos/química , Coelhos , Radiografia , Transplante Autólogo , Resultado do TratamentoRESUMO
PURPOSE: Morphological study comparing castor oil polyurethane and autogenous bone graft to repair bone defect in zygomatic bone of rabbits. METHODS: Twenty-four adult, male New Zealand rabbits were randomly distributed between two groups of twelve. Bone defects of 5mm in diameter were cut through the zygomatic bone and filled with polyurethane discs in the experimental group or autogenous bone harvested from the tibia in the control group. Animals were sacrificed after 30, 60 or 90 days, and the zygomatic bones were macro- and microscopically analyzed. Student's, Fisher's, chi-squared and McNemar's tests were used for statistical analysis. RESULTS: Both the castor oil polyurethane and the autograft adapted well to the defect, with no need for fixation. Fibrous connective tissue encapsulated the polyurethane, but no inflammation or giant cell reaction was observed. Acidophilic and basophilic areas were observed inside the micropores of the polyurethane, suggesting cell nuclei. After 90 days, bone repair with a lamellar pattern of organization was observed in the control group. CONCLUSION: The castor oil polyurethane was biocompatible and did not cause inflammation. It may be considered an alternative to fill bone defects.
OBJETIVO: Estudo morfológico comparativo do implante de poliuretana de mamona e enxerto ósseo autógeno em defeito ósseo padrão em osso zigomático de coelhos. MÉTODOS: Vinte e quatro coelhos Nova Zelândia, machos, adultos, foram distribuídos aleatoriamente em dois grupos de 12. Defeitos de 5mm de diâmetro, perenes, foram confeccionados em osso zigomático e preenchidos com discos pré-fabricados de poliuretana no grupo experimento ou osso autógeno extraído da tíbia no grupo controle. Os animais foram sacrificados após 30, 60 e 90 dias e as peças anatômicas foram avaliadas macro e microscopicamente. Foram utilizados os testes de Student, Fisher,qui-quadrado e McNemar para a análise estatística dos resultados. RESULTADOS: A poliuretana e o osso autógeno se adaptaram ao defeito sem necessidade de fixação. Houve formação de tecido conjuntivo fibroso envolvendo a poliuretana, sem reação inflamatória ou presença de células gigantes. Verificaram-se áreas acidófilas e basófilas nos poros do material implantado, sugestivas de núcleos celulares. No grupo controle, observou-se aos 90 dias o reparo ósseo de padrão lamelar clássico. CONCLUSÃO: A poliuretana de mamona foi biocompatível e não causou reação inflamatória deletéria. Pode ser uma alternativa para o preenchimento de defeitos ósseos.
Assuntos
Animais , Masculino , Coelhos , Materiais Biocompatíveis/uso terapêutico , Transplante Ósseo/métodos , Óleo de Rícino/uso terapêutico , Teste de Materiais , Poliuretanos/uso terapêutico , Zigoma/cirurgia , Peso Corporal , Substitutos Ósseos/uso terapêutico , Transplante Ósseo/patologia , Distribuição de Qui-Quadrado , Modelos Animais de Doenças , Distribuição Aleatória , Fatores de Tempo , Transplante Autólogo , Resultado do Tratamento , Tíbia/cirurgia , Zigoma/anormalidades , Zigoma/ultraestruturaRESUMO
PURPOSE: Morphological study comparing castor oil polyurethane and autogenous bone graft to repair bone defect in zygomatic bone of rabbits. METHODS: Twenty-four adult, male New Zealand rabbits were randomly distributed between two groups of twelve. Bone defects of 5mm in diameter were cut through the zygomatic bone and filled with polyurethane discs in the experimental group or autogenous bone harvested from the tibia in the control group. Animals were sacrificed after 30, 60 or 90 days, and the zygomatic bones were macro- and microscopically analyzed. Student's, Fisher's, chi-squared and McNemar's tests were used for statistical analysis. RESULTS: Both the castor oil polyurethane and the autograft adapted well to the defect, with no need for fixation. Fibrous connective tissue encapsulated the polyurethane, but no inflammation or giant cell reaction was observed. Acidophilic and basophilic areas were observed inside the micropores of the polyurethane, suggesting cell nuclei. After 90 days, bone repair with a lamellar pattern of organization was observed in the control group. CONCLUSION: The castor oil polyurethane was biocompatible and did not cause inflammation. It may be considered an alternative to fill bone defects.