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A ozonioterapia engloba o emprego do gás no setor saúde, advindo de suas características analgésicas, imunomoduladoras, antimicrobianas e imunoestimulantes, o que possibilita que ele atue no enfrentamento de inúmeras patologias. O uso do ozônio tem ocorrido nas áreas de Medicina e de Odontologia, advindo do fato de que esse gás é empregado sob concentrações baixas, o que caracteriza esse tipo de terapia como sendo um recurso seguro, que deve proceder por intermédio da aplicação efetuada por um profissional de saúde apto e capacitado para tal. O objetivo do presente artigo foi evidenciar como a ozonioterapia pode ser aplicada em âmbito odontológico. Em Odontologia, pode-se efetuar terapia com esse gás nas áreas de Periodontia, Dentística, Cirurgia e Endodontia. Sua aplicabilidade odontológica advém de suas propriedades antimicrobianas, curativas e imunometabólicas. A ozonioterapia corretamente aplicada e selecionada para uso odontológico pode possibilitar condições de obter-se um melhor desfecho no tratamento preconizado aos pacientes.
Ozone therapy encompasses the use of gas in the health sector, arising from its analgesic, immunomodulatory, antimicrobial and immunostimulant characteristics, which allows it to act in the fight against numerous pathologies. The use of ozone has occurred in the areas of Medicine and Dentistry, arising from the fact that this gas is used at low concentrations, which characterizes this type of therapy as a safe resource, which must proceed through the application carried out by a qualified and qualified health professional. The objective of this article was to show how ozone therapy can be applied in the dental field. In Dentistry, therapy with this gas can be carried out in the areas of Periodontics, Dentistry, Surgery and Endodontics. Its dental applicability comes from its antimicrobial, curative and immunometabolic properties. Ozone therapy correctly applied and selected for dental use can enable conditions to obtain a better outcome in the treatment recommended for patients.
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Terapias Complementares , Assistência Odontológica , Odontologia , OzonioterapiaRESUMO
The Metropolitan Area of São Paulo (MASP) in southern Brazil is impacted by high ozone levels posing significant threats to its urban forests and the Atlantic Forest remnants. These green areas, covering 540 km2 and constituting 30% of MASP's territory, necessitate an urgent assessment of air pollution impacts on their flora. Our study investigates the effects of atmospheric pollution on the morphoanatomical and physiological responses of four native tree species (Alchornea sidifolia, Casearia sylvestris, Guarea macrophylla, and Machaerium nyctitans) across two Atlantic Forest remnants in MASP. We examined visual and morphoanatomical changes in leaves, gas exchange, photosynthetic pigments, and plant volatile organic compounds to identify markers for biomonitoring urban environments. Our results reveal that MASP vegetation is adversely affected by tropospheric ozone. Species with porous mesophyll structures, such as M. nyctitans and G. macrophylla, exhibited greater visual and structural damage. In contrast, species with compact mesophyll, such as A. sidifolia and C. sylvestris, demonstrated higher tolerance. This suggests that anatomical architecture critically influences species' responses to atmospheric pollutants, such as tropospheric ozone. Additionally, we propose that ozone influx occurs through both stomatal pathways and as a result of direct and indirect injuries to the plant tissues. Additionally, our study identifies non-visual markers, including anatomical and physiological parameters and plant volatile organic compounds (e.g., presence of salicylates), as effective tools for monitoring plant species in urban environments. These insights highlight key anatomical and metabolic markers that help distinguish ozone-tolerant species from sensitive species, providing valuable information for monitoring air pollution in urban forests.
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Dissolving microneedles (DMNs) is a promising technology for transdermal and intradermal drug delivery. However, effective decontamination protocols are necessary to ensure safety and efficacy in clinical applications. The challenge is to use a technique that preserves mechanical properties, does not introduce chemicals, and can decontaminate DMNs without affecting the drug. With its potent antimicrobial properties and minimal residual effects, ozone presents a novel and safe method for decontaminating DMNs. Specifically, the present study assesses ozone's efficacy in decontaminating DMNs loaded with aminolevulic acid, intended for photodynamic therapy in skin cancer treatment. The results showed that it effectively decontaminates E. coli and S. aureus without compromising the polymer properties or promoting drug degradation. Overall, ozone represents an approach that can be adopted to decontaminate DMNs, offering a safer and effective strategy that enhances their potential to translate to clinical application.
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The reliance on agriculture in many nations has increased the use of treated wastewater for irrigation. However, reclaimed water still poses health risks from resistant pathogens like Cryptosporidium spp. Ozone, a strong disinfectant, has been used in water treatment. This study assessed the microbiological quality of treated wastewater for irrigation and evaluated ozone effectiveness in inactivating C. parvum oocysts. All samples contained Cryptosporidium spp., with 163 to 850 oocysts 100 L-1, and 50% contained viable oocysts. When C. parvum was exposed to different ozone residual concentrations (0.1, 0.8, and 1.3 mg L-1), oocyst viability reduction of 73%, 85%, and 99% and infectivity of 0.8, 1.36, and 2 Log10 was achieved. The predicted values for infectious oocysts were 4.19, 3.64, and 3.27, representing absolute counts of infective oocysts after ozone treatment. These findings demonstrate ozone's effectiveness in inactivating C. parvum in treated wastewater, supporting its potential for safe water reuse. PRACTITIONER POINTS: All wastewater samples contained Cryptosporidium spp., with 163 to 850 oocysts per 100 L. Wastewater had 50% contained viable oocysts. Ozone concentrations (0.1, 0.8, 1.3 mg/l) achieved oocyst viability of 73.33%, 85.0%, and 99.4%, respectively. The predicted values for infectious oocysts were 4.19, 3.64, and 3.27, respectively for each ozone concentration.
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Irrigação Agrícola , Cryptosporidium parvum , Desinfecção , Ozônio , Águas Residuárias , Purificação da Água , Ozônio/farmacologia , Cryptosporidium parvum/efeitos dos fármacos , Águas Residuárias/parasitologia , Águas Residuárias/química , Desinfecção/métodos , Purificação da Água/métodos , Oocistos/efeitos dos fármacos , Eliminação de Resíduos Líquidos/métodosRESUMO
The dairy industry faces challenges in controlling spoilage microorganisms, particularly Pseudomonas, known to form resilient biofilms. Conventional disinfection methods have limitations, prompting the exploration of eco-friendly alternatives like ozone. This study focused on Pseudomonas biofilms on polystyrene and polyethylene surfaces, evaluating ozone efficacy when incorporated into different water sources and applied under static and dynamic conditions. Biofilm formation and removal were assessed with conventional microbiological and microscopic techniques. Despite variations in physicochemical properties, ozonized water from different sources showed similar effectiveness in removing Pseudomonas biofilms. Dynamic ozone application was more efficient, achieving a 2.35 log CFU/coupon reduction on polyethylene surfaces, compared to a 1.05 log CFU/coupon reduction under static conditions. These findings highlight the potential of ozonized water for removing Pseudomonas biofilms, especially under dynamic application. This eco-friendly approach could serve as an effective strategy to mitigate biofilm-related challenges in the dairy industry.
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Biofilmes , Ozônio , Polietileno , Poliestirenos , Pseudomonas , Biofilmes/efeitos dos fármacos , Poliestirenos/química , Polietileno/química , Pseudomonas/fisiologia , Ozônio/farmacologia , Ozônio/química , Água/química , Desinfecção/métodos , Propriedades de SuperfícieRESUMO
OBJECTIVE: Studies have suggested that ozone therapy stimulates antioxidant enzyme expression and activity, regulates growth factor production, increases local blood flow and provides antibacterial protection. We sought to analyse the evidence related to the use of ozone therapy in the treatment of diabetic foot ulcers (DFUs). METHOD: A systematic review and meta-analysis was conducted using published clinical trials indexed in the PubMed, Embase and Web of Science databases up to December 2020. Outcomes analysed included: number of ulcers cured; ulcer area reduction; length of hospital stay; changes in blood glucose levels; antioxidant activity; and number of amputations. RESULTS: Initially, 61 studies were identified; however, only six met all of the eligibility criteria for inclusion in the final sample. It was found that patients receiving ozone therapy displayed higher rates of healing (i.e., healed ulcers), shorter hospital stays, increased catalase activity and reduced blood glucose levels. There was an observed increase in the number of ulcers healed; however, there was a considerable risk of bias in the studies evaluated. CONCLUSION: Although many studies have suggested ozone therapy as a promising candidate in the treatment of DFUs, further research is required, with a better scientific outline, to validate the results.
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Pé Diabético , Ozônio , Cicatrização , Ozônio/uso terapêutico , Humanos , Pé Diabético/terapia , Pé Diabético/tratamento farmacológico , Cicatrização/efeitos dos fármacos , Resultado do Tratamento , Tempo de Internação/estatística & dados numéricosRESUMO
This study aimed to identify whether there is an ideal concentration for applying ozonized oil (OZ) in the post-exodontic alveoli of senescent rats treated with zoledronate (ZOL). Thirty-five female rats, aged 18 months, were divided into five groups: ZOL; ZOL+OZ500; ZOL+OZ600; ZOL+OZ700; and SAL. The groups treated with ZOL, and other concentrations of OZ received applications at a dose of 100 µg/kg, while the SAL group received saline. After three weeks of ZOL application, the animals underwent extraction of the lower first molar. Subsequently, local therapies were initiated: group ZOL+OZ500 at 500 mEq/kg; ZOL+Z600 at 600 mEq/kg; and ZOL+OZ700 at 700 mEq/kg at baseline, and on days 2 and 4 post-operation. Euthanasia was performed on day 28. The microtomographic parameter of bone volume and histometric data on the area of neoformed bone (NFBT) showed the highest values for the ZOL+OZ600 group (p < 0.05). All OZ groups had smaller areas of non-vital bone than the ZOL group (p < 0.05). The clinical appearance of the operated region showed the alveoli covered with soft tissue, particularly in the OZ groups. All the tested concentrations of OZ were able to prevent and modulate MRONJ. As it presents a greater amount of NFBT, the concentration of 600 mEq/kg seems to be ideal.
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Peri-urban conserved natural or semi-natural areas provide several ecosystem services and assist in reducing air pollution in cities. The aim of this study is to assess the contribution to the improvement of air quality of a small area (<1 km2) adjacent to a city in the Metropolitan Region of Rio de Janeiro (Brazil), which is seriously affected by vehicular and industrial emissions of pollutants. Hydrocarbon (HC) and carbonyl compounds (CC) levels were determined, by employing TO-15 and TO-11A US EPA Methods, respectively, in both the urban and green areas. The results showed that the concentrations of anthropogenic HC were approximately 1.7-2.1 times higher in the urban area which confirms that the natural park assists in the dispersion and reduction of pollutants. In the case of the CC compounds, for samples that were collected in the morning, the total mean and median values were 1.3-1.6 times higher in the urbanized zone, while during the afternoon the green area showed values that were 1.5-1.9 times higher. These results suggest that in the green area, the emission or formation of CC compounds through photochemical processes is significant, particularly in the afternoon. Anyway, the ozone forming potential was found to be lower within the natural park in both periods, which confirms the positive role played by conserved natural areas outside or surrounding extensive metropolitan areas in the reduction of atmospheric pollution, in spite of the negative impact of anthropogenic emissions.
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This study sought to examine the effects of systemic ozone (O3) treatment on the healing of skin wounds induced on the dorsal surface of Wistar rats. The skin wounds were created using a 10 mm round punch following the sagittal medial plane in 72 rats. Then, the animals were randomly assigned to four groups, each receiving the following treatments: group C, which did not undergo treatment with the O3/O2 mixture; group OZ0.3, administered the O3/O2 mixture at a dose of 0.3 mg/kg; group OZ0.7, given the O3/O2 mixture at a dose of 0.7 mg/kg; and group OZ1.0, provided with the O3/O2 mixture at a dose of 1.0 mg/kg. Six animals from each group were euthanized at 7, 14, and 21 days postoperatively. Clinical, histological, histometric, and immunohistochemical (IHC) analyses were accomplished. Data from clinical and histometric assessments revealed that OZ0.7 and OZ1.0 demonstrated more favorable healing, with greater wound contraction observed in the OZ1.0 group at 14 and 21 days. Histologically, the OZ1.0 group exhibited aspects consistent with an accelerated tissue repair process. IHC analysis revealed greater vascular endothelial growth factor (VEGF) immunostaining in the OZ0.7 (7 days) and OZ1.0 (7 and 14 days) groups compared to the C group. Expression of transforming growth factor beta-1 was significantly increased in the OZ0.7 (14 days) and OZ1.0 (7 and 14 days) groups compared to the C group. In conclusion, our data suggest that systemic use of O3 enhanced tissue repair in cutaneous wounds in a dose-dependent manner, with concentrations of 1.0 mg/kg providing the most beneficial effects. Furthermore, the results of this study implicate the use of O3 for the treatment of skin wounds aiming at improving the healing process over time. Our findings suggest the use of O3 as a viable alternative to enhance wound healing and repair.
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Ozônio , Ratos Wistar , Pele , Cicatrização , Animais , Ozônio/farmacologia , Cicatrização/efeitos dos fármacos , Pele/efeitos dos fármacos , Pele/lesões , Pele/metabolismo , Pele/patologia , Ratos , Masculino , Imuno-Histoquímica , Fator A de Crescimento do Endotélio Vascular/metabolismo , Fator de Crescimento Transformador beta1/metabolismoRESUMO
Dementia encompasses a spectrum of neurodegenerative disorders significantly impacting global health, with environmental factors increasingly recognized as crucial in their etiology. Among these, ozone, has been identified as a potential exacerbator of neurodegenerative processes, particularly in Alzheimer's disease (AD). Ozone exposure induces the production of reactive oxygen species (ROS), which penetrate the BBB, leading to oxidative damage in neuronal cells. This oxidative stress is closely linked with mitochondrial dysfunction and lipid peroxidation, processes that are foundational to the pathology observed in dementia, such as neuronal death and protein aggregation. Furthermore, ozone triggers chronic neuroinflammation, exacerbating these neurodegenerative processes and perpetuating a cycle of CNS damage. Recent studies highlight the role of peripheral biomarkers like High Mobility Group Box 1 (HMGB1) and Triggering Receptor Expressed on Myeloid cells 2 (TREM2) in mediating ozone's effects. Disruption of these and other identified proteins by ozone exposure impairs microglial function and response to amyloid plaques, suggesting a novel pathway through which ozone may influence AD pathology via immune dysregulation. This review discusses the concept of a bidirectional lung-brain axis, illustrating that systemic responses to air pollutants like ozone may reflect and contribute to neurodegenerative processes in the CNS. By delineating these mechanisms, we emphasize the critical need for integrating environmental health management into strategies for the prevention and treatment of dementia.
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This study aimed to evaluate the effectiveness of ozonation in controlling Pseudomonas spp. biofilm in the food industry, and present possible parameters influencing this process. The study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The search was conducted in the PubMed, EMBASE, ScienceDirect, and Scopus databases. Eleven articles published between 1993 and 2023 were included in the study, indicating that the topic has been under investigation for several decades, gaining more prominence in recent years. Studies have demonstrated the antimicrobial effect of ozone under different experimental conditions, indicating that it is an effective strategy. Furthermore, they suggest that, in addition to ozone concentration and exposure time, other parameters such as the type of materials used in processing plants, hydrodynamic conditions, water temperature, and knowledge of commonly found microorganisms contribute to the effectiveness of the process aimed at reducing microbial counts. In conclusion, the available evidence suggests that ozonation in controlling Pseudomonas spp. can be considered a promising antimicrobial strategy. More efforts are needed to adapt the different methodologies according to each industrial reality.
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Biofilmes , Indústria Alimentícia , Ozônio , Pseudomonas , Ozônio/farmacologia , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Pseudomonas/efeitos dos fármacos , Pseudomonas/fisiologia , Indústria Alimentícia/métodosRESUMO
BACKGROUND: Stroke is a leading cause of death worldwide, with oxidative stress and calcium overload playing significant roles in the pathophysiology of the disease. Ozone, renowned for its potent antioxidant properties, is commonly employed as an adjuvant therapy in clinical settings. Nevertheless, it remains unclear whether ozone therapy on parthanatos in cerebral ischemia-reperfusion injury (CIRI). This study aims to investigate the impact of ozone therapy on reducing parthanatos during CIRI and to elucidate the underlying mechanism. METHODS: Hydrogen peroxide (H2O2) was utilized to mimic the generation of reactive oxygen species (ROS) in SH-SY5Y cell reperfusion injury in vitro, and an in vivo ischemic stroke model was established. Ozone saline was introduced for co-culture or intravenously administered to mice. Apoptosis and oxidative stress were assessed using flow cytometry and immunofluorescence. Western blotting was utilized to examine the expression of parthanatos signature proteins. The mechanism by which ozone inhibits parthanatos was elucidated through inhibiting PPARg or Nrf2 activity. RESULTS: The findings demonstrated that ozone mitigated H2O2-induced parthanatos by either upregulating nuclear factor erythroid 2-related factor 2 (Nrf2) or activating peroxisome proliferator-activated receptorg (PPARg). Furthermore, through the use of calcium chelators and ROS inhibitors, it was discovered that ROS directly induced parthanatos and facilitated intracellular calcium elevation. Notably, a malignant feedback loop between ROS and calcium was identified, further amplifying the induction of parthanatos. Ozone therapy exhibited its efficacy by increasing PPARg activity or enhancing the Nrf2 translation, thereby inhibiting ROS production induced by H2O2. Concurrently, our study demonstrated that ozone treatment markedly inhibited parthanatos in stroke-afflicted mice. Additionally, ozone therapy demonstrated significant neuroprotective effects on cortical neurons, effectively suppressing parthanatos. CONCLUSIONS: These findings contribute valuable insights into the potential of ozone therapy as a therapeutic strategy for reducing parthanatos during CIRI, highlighting its impact on key molecular pathways associated with oxidative stress and calcium regulation.
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Modelos Animais de Doenças , AVC Isquêmico , Estresse Oxidativo , Ozônio , Espécies Reativas de Oxigênio , Ozônio/farmacologia , Ozônio/uso terapêutico , Animais , AVC Isquêmico/tratamento farmacológico , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Traumatismo por Reperfusão , Masculino , Peróxido de Hidrogênio/metabolismo , Humanos , Fator 2 Relacionado a NF-E2/metabolismo , Apoptose/efeitos dos fármacos , Camundongos Endogâmicos C57BL , Cálcio/metabolismoRESUMO
Background: Drug therapies have been widely applied for pain management, however, there are important side effects such as those related to corticosteroids and opioids. Recent studies demonstrated promising results using medical ozone as a safe, effective, and low-cost intervention for pain control. Objective: to review and critically analyze clinical studies that used ozone therapy for musculoskeletal pain. Methods: a literature search of various databases was performed to identify relevant studies. From a total of 249 records, 27 studies were included. Quality indicators, human and device factors that strongly influence the generation of evidence were considered, such as study design and device safety. We also mitigated biases, considering the safety and efficacy of the intervention itself. Results: Regarding safety, 77 (8%) of studies reported no adverse effects; concerning efficacy outcomes, medical ozone shows to be an effective intervention on musculoskeletal pain control. Important information about used devices were missing. Conclusions: medical ozone shows to be safe and effective; qualification of health professionals as well as the device safety are mandatory. However, there is a lack of requirements to identify the best therapeutic scheme; further longer, clinical and rigorous trials are needed.
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Dor Musculoesquelética , Ozônio , Manejo da Dor , Humanos , Ozônio/uso terapêutico , Dor Musculoesquelética/tratamento farmacológico , Manejo da Dor/métodos , Ensaios Clínicos como Assunto , Indicadores de Qualidade em Assistência à SaúdeRESUMO
Ambient air pollution is a significant environmental risk factor for adverse pregnancy outcomes, including preterm birth. However, the impact of different pollutants across various regions and trimesters of pregnancy has not been fully investigated in Brazil. This study aimed to examine the associations between exposure to PM2.5, NO2, and O3 during different trimesters of pregnancy and the risk of preterm birth across five regions of Brazil. We used logistic regression models to estimate the odds ratios (OR) of preterm birth associated with PM2.5, NO2, and O3 adjusting for potential confounders such as maternal age, education, and socioeconomic status. Our study included over 9.9 million live births from 2001 to 2018, with data obtained from the Ministry of Health in Brazil. On average, for each 1-µg/m3 increase in PM2.5, we estimated a 0.26â¯% (95â¯% CI: 0.08-0.44â¯%) increase in the risk of preterm birth nationally in the first trimester. For NO2, each 1ppb increase was associated with a percentage increase in preterm birth risk of 7.26â¯% (95â¯% CI: 4.77-9.74â¯%) in the first trimester, 8.05â¯% (95â¯% CI: 5.73-10.38â¯%) in the second trimester, and 7.48â¯% (95â¯% CI: 5.25-9.72â¯%) in the third trimester. For O3, each 1ppb increase was associated with a percentage increase in preterm birth risk of 1.24â¯% (95â¯% CI: 0.29-2.18â¯%) in the first trimester, 1.51â¯% (95â¯% CI: 0.60-2.41â¯%) in the second trimester, and 0.72â¯% (95â¯% CI: -0.18-1.62â¯%) in the third trimester. This study highlights the significant impact of ambient air pollution on preterm birth risk in Brazil, with significant regional variations. Our findings underscore the need for targeted public health interventions to mitigate the effects of air pollution on pregnancy outcomes, particularly in the most affected regions.
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Poluentes Atmosféricos , Poluição do Ar , Exposição Materna , Nascimento Prematuro , Gravidez , Nascimento Prematuro/epidemiologia , Feminino , Brasil/epidemiologia , Humanos , Poluição do Ar/efeitos adversos , Poluição do Ar/estatística & dados numéricos , Poluentes Atmosféricos/análise , Adulto , Exposição Materna/estatística & dados numéricos , Exposição Materna/efeitos adversos , Material Particulado/análise , Adulto Jovem , Ozônio/análise , Dióxido de Nitrogênio/análiseRESUMO
The aim of this study was to evaluate whether a system involving ozonated water and ultrasound causes de-epithelization of the human amniotic membrane (HAM). The experiment protocol was carried out in four stages. Stage I was carried out to determine the duration of the experiment. Stage II comprised the first experiment, involving four groups of samples studied in triplicate: control/natural (IN), processed with ultrasound in a liquid medium (US), processed with ozonated water (O3), and processed with ozonated water combined with ultrasound (US_O3). Stage III was performed to confirm the results, following the same steps present in Stage II. Stage IV involved the use of oxygen to confirm the hypothesis. Histological analysis was carried out to verify whether the effects of O2 were similar to those of O3. The system was activated, and ozonation was carried out for 10 min, as in the previous experiment, reaching a concentration level of 3.0 mg/L. The samples were submerged and positioned in the reservoir and processed separately for 55 min. The biochemical properties were assessed using Fourier transform infrared spectroscopy, and the morphology was examined using histology and scanning electron microscopy. The spectra of the samples exhibited similarities; however, subtle changes were highlighted, such as smooth band shifts and intensity changes. The morphology indicated that ultrasound achieved more efficient HAM de-epithelialization compared to ultrasound combined with ozonated water and ozonated water alone. One plausible hypothesis for this observation is that cavitation represents the primary mechanism responsible for de-epithelialization. When ultrasound is combined with ozone, the bubbles generated by ozone gas reduce the cavitation effect. This study is pioneering as it demonstrates an ultrasound system capable of the efficient de-epithelialization of the HAM.
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Despite a growing literature for complex air quality models, scientific evidence lacks of the influences of varying exposure assessments and air quality data sources on the estimated mortality risks. This case-crossover study estimated cardiovascular mortality risks from fine particulate matter (PM2.5) and ozone (O3) exposures, using varying exposure methods, to aid understanding of the impact of exposure methods in the health risk estimation. We used individual-level cardiovascular mortality data in the city of Rio de Janeiro, 2012-2016. PM2.5 and O3 exposure levels (from the date of death to seven prior days [lag0-7]) were estimated at the individual level or district level using either the WRF-Chem modeling data or monitoring data, resulting in a total of 10 exposure methods. The exposure-response relationships were estimated using multiple logistic regressions. The changes in cardiovascular mortality were represented as an odds ratio (OR) and 95% confidence intervals (CIs) for an interquartile range (IQR) increase in the exposures. Results showed that socioeconomically more advantaged populations had lower access to the stationary monitoring networks. Higher variance in the estimated exposure levels across the 10 exposure methods was found for PM2.5 than O3. PM2.5 exposure was not associated with mortality risk in any exposure methods. WRF-Chem-based O3 exposure estimated for each individual of the entire population found a significant mortality risk (OR = 1.06, 95% CI: 1.01, 1.11), but not the other exposure methods. Higher risks for females and older populations were suggested for O3 estimates estimated for each individual using the WRF-Chem data. Findings indicate that decisions on exposure methods and data sources can lead to substantially varying implications for air pollution risks and highlight the need for comprehensive exposure and health impact assessments to aid local decision-making for air pollution and public health.
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Poluentes Atmosféricos , Poluição do Ar , Doenças Cardiovasculares , Exposição Ambiental , Ozônio , Material Particulado , Humanos , Doenças Cardiovasculares/mortalidade , Brasil/epidemiologia , Material Particulado/análise , Exposição Ambiental/efeitos adversos , Ozônio/análise , Ozônio/efeitos adversos , Feminino , Masculino , Pessoa de Meia-Idade , Poluição do Ar/efeitos adversos , Poluição do Ar/análise , Poluentes Atmosféricos/análise , Idoso , Adulto , Cidades/epidemiologia , Adulto Jovem , Estudos Cross-Over , Medição de Risco , Idoso de 80 Anos ou maisRESUMO
OBJECTIVES: To assess the effectiveness of ozone therapy in guided bone regeneration (GBR) for critical size calvarial defects in rats. MATERIALS AND METHODS: 96 male Wistar rats were divided into four groups (n = 6 each). An 8 mm critical defect was created in the calvaria of each rat. The groups were: BIO (porcine collagen membrane, BioGide®), BIO + OZ (membrane with systemic ozone therapy every 2 days), COA + OZ (blood clot with ozone therapy), and COA (blood clot only). Evaluations at 7, 15, 30, and 60 days included histological, histomorphometric, inflammatory profile, Micro-CT, and immunohistochemical analyses. Statistical analysis involved two-factor ANOVA with Tukey's post-hoc test for general data, and one-factor ANOVA with Holm-Sidak post-hoc test for Micro-CT data. RESULTS: The BIO + OZ group demonstrated superior bone regeneration with well-organized, mature bone tissue and significant bone formation at 30 and 60 days. The COA + OZ group showed early angiogenesis and reduced inflammation, resulting in complete defect closure by 30 days. The BIO group had good regeneration, but less mature tissue compared to BIO + OZ. The COA group exhibited limited bone formation and higher porosity. CONCLUSION: Ozone therapy positively influences bone regeneration by enhancing cell proliferation and the healing response. CLINICAL RELEVANCE: Improving regenerative processes with auxiliary therapies like ozone therapy can be significant for advancing dental reconstructions.
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Regeneração Óssea , Regeneração Tecidual Guiada , Imuno-Histoquímica , Ozônio , Ratos Wistar , Crânio , Microtomografia por Raio-X , Animais , Ozônio/uso terapêutico , Ratos , Masculino , Regeneração Óssea/efeitos dos fármacos , Regeneração Tecidual Guiada/métodos , Colágeno , Membranas ArtificiaisRESUMO
This ex vivo study devised an analytical ex vivo method for infection/disinfection of simulated lateral canals located in the middle and apical segments of the root. The antibacterial effects of supplementary approaches were tested in this model. Extracted mandibular premolars had their main root canals enlarged and then two lateral canals (100 µm in diameter) were created in the root, one in the apical and the other in the middle portion. Micro-computed tomography was used for specimen selection and to confirm the quality of the simulated ramifications. The specimens were contaminated with a mixed bacterial culture from subgingival bacterial biofilm added to pure Enterococcus faecalis strain ATCC 29212 grown overnight, using special strategies to facilitate culture medium penetration within the lateral canals. The following procedures were tested for disinfection: NaOCl/passive ultrasonic irrigation (PUI), NaOCl/XP-endo Finisher, ozonated water/continuous ultrasonic irrigation (CUI), and NaOCl/conventional irrigation with 30-G needles (control). Bacteriological samples were taken from the main canal before (S1) and after (S2) each supplementary protocol, and also from each lateral canal after treatment (S3). DNA extracted from the samples was subjected to quantitative real-time polymerase chain reaction. All S1 main canal samples were positive for bacterial presence. Bacterial counts in the main root canal substantially decreased by 99.2% after PUI, 99.1% after ozone/CUI, 99% after XP-endo Finisher, and 96% in the control group (P < 0.01 for all groups). There were no significant differences between groups (P > 0.05). The same was observed when comparing the effects of the supplementary approaches in the apical and middle lateral canals (P > 0.05). Only a few lateral canals showed no detectable bacteria. The method proposed here proved effective for ex vivo infection/disinfection studies. All supplementary approaches induced a substantial bacterial reduction in the main canal, with no significant differences between them. However, in terms of lateral canal disinfection, none of the tested approaches showed significant effects when compared to the control group.
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Cavidade Pulpar , Desinfecção , Enterococcus faecalis , Hipoclorito de Sódio , Cavidade Pulpar/microbiologia , Humanos , Desinfecção/métodos , Hipoclorito de Sódio/farmacologia , Enterococcus faecalis/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Irrigantes do Canal Radicular/farmacologia , Microtomografia por Raio-X , Preparo de Canal Radicular/métodosRESUMO
OBJECTIVES: To conduct a systematic review and meta-analysis to assess the effectiveness of ozone therapy in oral ulcers healing when compared to placebo or active treatments. MATERIALS AND METHODS: The search was carried out using PubMed, EMBASE, Scopus, and Lilacs databases. Clinical trials involving human participants were included. The Risk Ratio (RR) and the standardized mean difference (SMD) with 95%CI (confidence interval) were calculated. The ROBINS-I (risk of bias in non-randomized studies of interventions) and RoB2 (risk of bias tool for randomized trials) assessment tool was used to detect bias. RESULTS: After the selection process, 12 studies were included. The meta-analysis showed that ozone therapy helps to reduce the size of the traumatic and autoimmune ulcers (RR=-0.44; 95% CI -0.71,-0.17; I2=0%) in comparison to placebo. Regarding pain reduction, ozone was superior to placebo (RR = 1.29, 95% CI -1.6 to -0.95); I2=0%), and equivalent to topical corticosteroid and laser photobiomodulation (RR = 0.26, 95% CI -0.27,0.78, p = 0.34). CONCLUSION: Ozone therapy is an alternative for accelerating healing and reducing pain for both traumatic and autoimmune ulcers. However, the quality of evidence is limited. CLINICAL RELEVANCE: Oral ulcerations are usually painful and impact quality of life requiring different approaches to boost wound healing and reduce symptoms. For this purpose, ozone therapy is a promising strategy.
Assuntos
Ozônio , Cicatrização , Ozônio/uso terapêutico , Humanos , Cicatrização/efeitos dos fármacos , Úlceras Orais/tratamento farmacológico , Úlceras Orais/terapia , Mucosa Bucal/efeitos dos fármacosRESUMO
Reclaimed water poses environmental and human health risks due to residual organic micropollutants and pathogens. Ozonation of reclaimed water to control pathogens and trace organics is an important step in advanced water treatment systems for potable reuse of reclaimed water. Ensuring efficient pathogen reduction while controlling disinfection byproducts remains a significant challenge to implementing ozonation in reclaimed water reuse applications. This study aimed to investigate ozonation conditions using a plug flow reactor (PFR) to achieve effective pathogen removal/inactivation while minimizing bromate and N-Nitrosodimethylamine (NDMA) formation. The pilot scale study was conducted using three doses of ozone (0.7, 1.0 and 1.4 ozone/total organic carbon (O3/TOC) ratio) to determine the disinfection performance using actual reclaimed water. The disinfection efficiency was assessed by measuring total coliforms, Escherichia coli (E. coli), Pepper Mild Mottle Virus (PMMoV), Tomato Brown Rugose Fruit Virus (ToBRFV) and Norovirus (HNoV). The ozone CT values ranged from 1.60 to 13.62 mg min L-1, resulting in significant reductions in pathogens and indicators. Specifically, ozone treatment led to concentration reductions of 2.46-2.89, 2.03-2.18, 0.46-1.63, 2.23-2.64 and > 4 log for total coliforms, E. coli, PMMoV, ToBRFV, and HNoV, respectively. After ozonation, concentrations of bromate and NDMA increased, reaching levels between 2.8 and 12.0 µg L-1, and 28-40.0 ng L-1, respectively, for average feed water bromide levels of 86.7 ± 1.8 µg L-1 and TOC levels of 7.2 ± 0.1 mg L-1. The increases in DBP formation were pronounced with higher ozone dosages, possibly requiring removal/control in subsequent treatment steps in some potable reuse applications.