RESUMO
The failures in Trichomonas vaginalis (TV) infection diagnosis leave more than half of cases unidentified. In this report, urine and vaginal discharge samples were analyzed by wet mount, culture examination, and real-time PCR by Allplex™ (Seegene®) kit, in a population assisted by the Brazilian Public Health System. From 747 samples, 2.81% were positive for TV in wet mount and culture, and 3.88% by Allplex™. Samples kept at - 80 ºC for 22 months did not impair the PCR technique. The sensitivity for wet mount, culture, and Allplex™ was 72, 100, and 100%, respectively. Allplex™ technique showed highest detection of TV.
Assuntos
Infecções Sexualmente Transmissíveis , Vaginite por Trichomonas , Trichomonas vaginalis , Feminino , Humanos , Trichomonas vaginalis/genética , Vaginite por Trichomonas/diagnóstico , Vaginite por Trichomonas/epidemiologia , Brasil/epidemiologia , Saúde Pública , Sensibilidade e Especificidade , Reação em Cadeia da Polimerase em Tempo Real , Infecções Sexualmente Transmissíveis/diagnóstico , Infecções Sexualmente Transmissíveis/epidemiologiaRESUMO
Se presentan 2 casos de bacteriemia por Helicobacter cinaedi. El primero se diagnosticó en un varón de 76 años y resultó secundario a la colocación de un acceso vascular; el segundo correspondió a un lactante febril de 37 días de vida, asociado a un cuadro de gastroen-terocolitis aguda. H. cinaedi es un microorganismo que presenta dificultad para desarrollarse en diferentes medios de cultivo y lograr su identificación a nivel de especie. En ambos casos fue fundamental la observación microscópica en fresco de las botellas de hemocultivo, la utilización de la espectrometría de masas y la posterior secuenciación del gen hsp60 para llegar a esa instancia. En los últimos anos se han informado infecciones por H. cinaedi con frecuencia creciente en otras partes del mundo. En este trabajo presentamos los primeros casos de bacteriemia por H. cinaedi documentados en Argentina.
Two cases of bacteremia caused by Helicobacter cinaedi are presented. The first case was diagnosed in a 76-year-old male patient, and was secondary to a vascular access device placement; the second case corresponded to a febrile infant of 37 days of life, and was associated with acute gastroenteritis. H. cinaedi is a microorganism difficult to grow in different culture media and also to identify to species level. In both cases, the microscopic observation of blood culture bottles, the use of mass spectrometry and the subsequent sequencing of the hsp60 gene were essential. In the recent literature, H. cinaedi infections are being reported more frequently. In this report we present the first documented cases of bacteremia caused by H. cinaedi in Argentina.
Assuntos
Humanos , Masculino , Lactente , Idoso , Infecções por Helicobacter/diagnóstico , Bacteriemia/diagnóstico , Argentina/epidemiologia , Espectrometria de Massas/métodos , Hemocultura/métodosRESUMO
Two cases of bacteremia caused by Helicobacter cinaedi are presented. The first case was diagnosed in a 76-year-old male patient, and was secondary to a vascular access device placement; the second case corresponded to a febrile infant of 37 days of life, and was associated with acute gastroenteritis. H. cinaedi is a microorganism difficult to grow in different culture media and also to identify to species level. In both cases, the microscopic observation of blood culture bottles, the use of mass spectrometry and the subsequent sequencing of the hsp60 gene were essential. In the recent literature, H. cinaedi infections are being reported more frequently. In this report we present the first documented cases of bacteremia caused by H. cinaedi in Argentina.
Assuntos
Bacteriemia/microbiologia , Infecções por Helicobacter/complicações , Helicobacter , Idoso , Argentina , Bacteriemia/diagnóstico , Infecções por Helicobacter/diagnóstico , Humanos , Lactente , MasculinoRESUMO
OBJECTIVES: Trichomonas vaginalis (TV) infection is the most common non-viral STI globally and can result in adverse pregnancy outcomes and exacerbated HIV acquisition/transmission. Nucleic acid amplification tests (NAATs) are the most sensitive diagnostic tests, with high specificity, but TV NAATs are rarely used in Brazil. We investigated the TV prevalence and compared the performance of the US Food and Drug Association-cleared Aptima TV assay with microscopy (wet mount and Gram-stained) and culture for TV detection in women in Pelotas, Brazil in an observational study. METHODS: From August 2015 to December 2016, 499 consecutive asymptomatic and symptomatic sexually active women attending a Gynaecology and Obstetrics Outpatient Clinic were enrolled. Vaginal fluid and swab specimens were collected and wet mount microscopy, Gram-stained microscopy, culture and the Aptima TV assay performed. RESULTS: The median age of enrolled women was 36.5 years (range: 15-77). The majority were white, had a steady sexual partner and low levels of education. The TV detection rate was 4.2%, 2.4%, 1.2% and 0% using the Aptima TV assay, culture, wet mount microscopy and Gram-stained microscopy, respectively. The sensitivity of culture and wet mount microscopy was only 57.1% (95% CI 36.5 to 75.5) and 28.6% (95% CI 13.8 to 50.0), respectively. CONCLUSIONS: A 4.2% positivity rate of T. vaginalis was found among women in Pelotas, Brazil and the routine diagnostic test (wet mount microscopy) and culture had low sensitivities. More sensitive diagnostic tests (NAATs) and enhanced testing of symptomatic and asymptomatic at-risk women are crucial to mitigate the transmission of TV infection, TV-associated sequelae and enhanced HIV acquisition and transmission.
Assuntos
Microscopia/normas , Kit de Reagentes para Diagnóstico/normas , Tricomoníase/diagnóstico , Vaginite por Trichomonas/diagnóstico , Trichomonas vaginalis/isolamento & purificação , Adolescente , Adulto , Idoso , Brasil/epidemiologia , Ensaio de Unidades Formadoras de Colônias/métodos , Ensaio de Unidades Formadoras de Colônias/normas , Erros de Diagnóstico , Feminino , Violeta Genciana , Humanos , Microscopia/métodos , Pessoa de Meia-Idade , Fenazinas , Prevalência , Sensibilidade e Especificidade , Tricomoníase/epidemiologia , Vaginite por Trichomonas/epidemiologia , Estados Unidos , United States Food and Drug Administration , Adulto JovemRESUMO
BACKGROUND: Trichomonas vaginalis (TV) is one of the most common pathogens causing sexually transmitted infections (STIs) in both men and women. Since there are no accurate prevalence data available on TV infection among women for Trinidad and Tobago and in the Caribbean region, there is a great need for research to study the dynamics of the infection and its transmission. OBJECTIVES: To determine the prevalence and diagnose TV infection in women attending the STI clinic and to provide recommendations for control and prevention of trichomoniasis in the community. STUDY DESIGN: A cross-sectional study was designed after obtaining the informed consent. Following a routine clinical examination, vaginal swabs were collected - one for wet mount preparation, one for InPouch culture, and other two for OSOM rapid test/and fluorescent antibody testing. Cotton swabs and Dacron swabs were used for testing. RESULTS: A prevalence of 16% of trichomoniasis out of 422 females was recorded. In all, 65 (65%) were in the age range of 15-29 years and peak of 30% in the age group of 20-24 years. Vaginal discharge was a common complaint (83%); 75.6% had foul odor and only 9.8% had typical fishy odor. The majority of them (87%) had whitish-yellow colored discharge. CONCLUSION: Prevalence of trichomoniasis is significant in the female population attending STI clinic. Targeting high-risk age group between 15 and 29 years for control and prevention would be beneficial. Prompt and accurate diagnosis using appropriate laboratory test is recommended.
RESUMO
OBJECTIVES: evaluating the level of information about the examination of uterine cervical cancer and its association with sociodemographic variables in women of a health care unit in the city of Bauru, São Paulo, Brazil. METHODS: we conducted a cross-sectional descriptive study with 370 women aged 25 to 59, through structured interviews in their own homes; we used descriptive statistics and the χ² test. RESULTS: 40.5% of the women had not undergone the Papanicolaou test at the recommended frequency; 58.2% incorrectly defined the test, and 69.5% did not know about the risk factors for the development of cervical cancer; the knowledge about the test showed statistically significant association with schooling and family income of the studied population. CONCLUSIONS: women present deficiencies on the proper practice of the Papanicolaou test, on knowledge about the test, risk factors and prevention methods. Therefore, it is necessary to develop primary health actions for the most vulnerable population...(AU)
OBJETIVOS: avaliar o nível de informação acerca do exame do câncer de colo de útero e sua associação com variáveis sócio-demográficas em mulheres de uma unidade de saúde do município de Bauru, São Paulo, Brasil. MÉTODO: realizou-se um estudo descritivo e transversal com 370 mulheres com idade entre 25 e 59 anos, por meio de entrevista estruturada nas próprias residências dos sujeitos e, foram utilizados a estatística descritiva e o teste χ². RESULTADOS: notou-se 40,5% não fizeram o exame Papanicolaou com a frequência recomendada; quanto ao conhecimento sobre o exame, 58,2% o definiram incorretamente e 69,5% não souberam relatar quais são os fatores de risco para o desenvolvimento do câncer de colo uterino; o conhecimento sobre o exame mostrou associação estatisticamente significativa com a escolaridade e a renda familiar da população estudada. CONCLUSÕES: as mulheres apresentam deficiências na prática adequada do exame de Papanicolaou, nos conhecimentos sobre o exame, os fatores de riscos e as formas de prevenção da doença e, diante disso, é necessário desenvolver ações primárias em saúde para a população mais vulnerável...(AU)
Assuntos
Humanos , Feminino , Mulheres , Neoplasias do Colo do Útero , Saúde da Mulher , Teste de Papanicolaou , Fatores de Risco , Atenção Primária à Saúde , Centros de Saúde , Serviços de Saúde da Mulher , Conhecimentos, Atitudes e Prática em Saúde , Estudos Transversais , Esfregaço Vaginal , Fatores SocioeconômicosRESUMO
OBJECTIVES: evaluating the level of information about the examination of uterine cervical cancer and its association with sociodemographic variables in women of a health care unit in the city of Bauru, São Paulo, Brazil. METHODS: we conducted a cross-sectional descriptive study with 370 women aged 25 to 59, through structured interviews in their own homes; we used descriptive statistics and the χ² test. RESULTS: 40.5% of the women had not undergone the Papanicolaou test at the recommended frequency; 58.2% incorrectly defined the test, and 69.5% did not know about the risk factors for the development of cervical cancer; the knowledge about the test showed statistically significant association with schooling and family income of the studied population. CONCLUSIONS: women present deficiencies on the proper practice of the Papanicolaou test, on knowledge about the test, risk factors and prevention methods. Therefore, it is necessary to develop primary health actions for the most vulnerable population...
OBJETIVOS: avaliar o nível de informação acerca do exame do câncer de colo de útero e sua associação com variáveis sócio-demográficas em mulheres de uma unidade de saúde do município de Bauru, São Paulo, Brasil. MÉTODO: realizou-se um estudo descritivo e transversal com 370 mulheres com idade entre 25 e 59 anos, por meio de entrevista estruturada nas próprias residências dos sujeitos e, foram utilizados a estatística descritiva e o teste χ². RESULTADOS: notou-se 40,5% não fizeram o exame Papanicolaou com a frequência recomendada; quanto ao conhecimento sobre o exame, 58,2% o definiram incorretamente e 69,5% não souberam relatar quais são os fatores de risco para o desenvolvimento do câncer de colo uterino; o conhecimento sobre o exame mostrou associação estatisticamente significativa com a escolaridade e a renda familiar da população estudada. CONCLUSÕES: as mulheres apresentam deficiências na prática adequada do exame de Papanicolaou, nos conhecimentos sobre o exame, os fatores de riscos e as formas de prevenção da doença e, diante disso, é necessário desenvolver ações primárias em saúde para a população mais vulnerável...