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The experiments reported in this research paper address the effects of replacing ground corn (GC) with full-fat corn germ (FFCG) on nutrient intake and digestibility, nitrogen utilization efficiency, performance, and predicted methane production in dairy cows fed cactus cladodes and sugarcane. We hypothesized that the inclusion of FFCG in the diet would not alter the performance of lactating cows but would reduce the predicted methane production in vivo. Ten multiparous Holstein cows at 90 ± 10 d of lactation and yielding 24.2 ± 3.5 kg milk/d were assigned to dietary treatments consisting of different levels of replacement of GC by FFCG (0; 25; 50; 75 and 100% of diet dry matter) in a replicated 5 × 5 Latin square design with 21-d periods. Methane production was predicted using an automated gas in vitro production system. Except for ether extract intake, which increased, the intake of all nutrients decreased linearly with the replacement of GC by FFCG. The digestibility of dry matter, organic matter and neutral detergent fiber reduced, whereas the digestibility of ether extract increased linearly with FFCG. There were no changes in the digestibility of crude protein. The nitrogen intake and daily excretion in urine and feces decreased, while nitrogen use efficiency increased linearly. There was no significant effect of diets on nitrogen balance or microbial protein synthesis and efficiency. The yield of protein, lactose and total solids in milk showed a quadratic behavior. On the other hand, milk fat yield and energy-corrected milk yield decreased linearly with the replacement of GC by FFCG. No effect on pH or ammonia nitrogen was observed. The production of methane (CH4, g/kg DM) and total CH4 (g/d), and CH4 intensity decreased linearly with the replacement of GC by FFCG. In conclusion, FFCG has been shown to be an effective source of fat to reduce methane production in dairy cows, partially supporting our initial hypothesis. However, as it decreases milk fat production, it is not recommended to replace more than 50% of GC by FFCG for lactating cows fed cactus cladodes and sugarcane.

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The objective of this study was to evaluate the inclusion of coffee pulp silage (CPS) on the performance and profitability of crossbred cows in the middle tropics. The research took place at the Villa Marina Experimental Farm of the University of Pamplona, Pamplonita, Colombia. Four lactating multiparous bovines with a body weight of 380 ± 10 kg, 6 ± 0.03 years of age and a body condition of 3.8 on a scale of 1 to 5. The animals were distributed in a balanced 4 × 4 Latin square for residual effect. The experiment consisted of four subperiods of 21 days each, being the first 14 days of adaptation to the experimental diet and 7 days for taking samples. The base diet consisted of Pennisetum sp. forage, concentrated in a proportion of 0.2% of body weight, water, and mineralized salt at will. The inclusion of the CPS was included in levels of 4, 8, and 12% of the dry matter (DM) intake. DM intake parameters, nutrient digestibility, milk production and composition, blood parameters, and economic analysis were evaluated. Treatments were compared using orthogonal contrasts; contrasts were constructed in order to evaluate the effects of inclusion of CPS, and the linear and quadratic effects of level inclusion in the DM of the diet. For the variables which did not present inclusion of CPS effect but a linear or quadratic effect was significant, a Dunnett's test was performed to identify whether a supplemented treatment differed from the control. Significance was considered at P < 0.05 and tendencies when 0.05 < P ≤ 0.10. The inclusion of CPS in the diet increases (P < 0.05) the voluntary intake in kg day-1 of DM, organic matter (OM), CP, ethereal extract (EE), non-fiber carbohydrates (NFC), digested OM (DM), neutral detergent fiber corrected for ash and protein (NDFap), DNDF and dietary NTD concentration, the total digestibility of DM and OM, and dietary DOM content. The production and chemical composition of milk was not affected (P > 0.10) by the inclusion of EPC in the diet. The inclusion of CPS increased (P < 0.05) the concentration of albumin and globulins in the blood. The inclusion of CPS in the diet improved the productive efficiency of dairy cattle. The inclusion of 4% CPS in the DM of the diet is a nutritional strategy, which improves the nutritional characteristics and the concentration of albumin and globulins in the blood of crossed dairy cows in the middle tropics and benefit: cost ratio without affecting milk production.

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Soil contamination caused by improper waste disposal can affect plant growth. Tropical forage plants have rapid growth, high biomass production and vigorous regrowth. Chromium (Cr) is one of the most common contaminants in the environment. Sulfur (S) is a nutrient involved in various cell detoxification processes. The objective was to assess the effects of excess Cr on biomass production and concentrations of this metal, S and cationic micronutrients, under conditions of varying supply of S for Tanzania guinea grass. The experiment was conducted in randomized complete blocks in a 3 × 4 factorial scheme, corresponding to three levels of S (0.1, 1.9 and 3.7 mmol L-1) and four levels of Cr (0.0, 0.5, 1.0 and 2.0 mmol L-1) in three replicates. Root surface area, shoot and root biomass production, concentrations of total S, sulfate-S, Cr and cationic micronutrients, and Cr transport factor were determined. S level of 1.9 mmol L-1 reduced the toxicity by Cr in Tanzania guinea grass, expressed by the higher shoot and root dry weight production, greater stability in the cellular concentration of sulfate-S and higher concentrations of micronutrients. It was concluded that the high availability of S reduced the toxicity by Cr(VI) in Tanzania guinea grass, expressed by the higher shoot and root dry weight production, greater stability in the cellular concentration of sulfate-S and higher concentration of micronutrients in the grass shoots.

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The aim of this study was to evaluate the performance and meat quality of lambs fed sugarcane silage containing increasing levels of detoxified castor bean meal (DCBM). Twenty-four Santa Inês male sheep were used, with initial body weight of 25.34 ± 2.57 kg, distributed in a completely randomized design with four treatments (0, 70, 140, and 210 g/kg of DCBM in the sugarcane silage). Intake and digestibility of dry matter (DM) and crude protein (CP) were positively influenced (P < 0.05) by the levels of DCBM. There was a linear increase (P < 0.05) on the intake and digestibility of neutral detergent fiber with the inclusion of DCBM. The daily and total gains showed a quadratic effect (P < 0.05) with the inclusion of DCBM, with a maximum point of 241.64 g/d and 18.12 kg at the level of 161.9 g/kg of DCBM. There was a linear increase (P < 0.01) on the hot carcass with the inclusion of DCBM. The inclusion of DCBM did not influence (P > 0.05) the levels of total lipids (2.1%) in lamb meat, but the levels of oleic acid (C18:1 n-6c) and total monounsaturated fatty acids presented a quadratic effect (P < 0.05). It is recommended to include 160 g/kg of detoxified castor bean meal in sugarcane silage.

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Objetivou-se comparar os teores dos nutrientes do capim Andropogon gayanus obtidos no equipamento NIRS com os valores obtidos por análises de via úmida, para identificar se os modelos globais de calibração do equipamento NIRS são adequados para predizer a composição químico-bromatológica. As análises de via seca no NIRS foram executadas no Laboratório de Bromatologia e Nutrição Animal (Laban), pertencente à Faculdade de Medicina Veterinária (Famev) da Universidade Federal de Uberlândia (UFU), enquanto as análises de via úmida foram realizadas no Laboratório de Química Analítica de Plantas da Embrapa Cerrados, localizado em Brasília-DF. As amostras utilizadas foram de capim Andropogon gayanus, cultivar Planaltina, as quais, após corte de 20cm acima do solo, foram secas e moídas no tamanho de 1mm. Após a moagem, foram analisadas por via úmida para os teores de matéria seca (MS), matéria mineral (MM), proteína bruta (PB), fibra insolúvel em detergente neutro (FDN) e fibra insolúvel em detergente ácido (FDA), segundo metodologias propostas por Detmann et al. (2012). As amostras moídas foram colocadas em cubetas próprias do equipamento e escaneadas em espectrômetro de refletância no infravermelho próximo (NIRS) NIR, modelo Spectra Star 2600 XT series of Near Infrared Analyzers (Unity Scientific®), em duplicata. Para obtenção dos teores de MS, MM, PB, FDN e FDA, empregaram-se modelos de calibração do próprio equipamento NIRS, utilizando-se a curva de calibração própria do equipamento. As médias dos teores de MS, MM, PB, FDN e FDA obtidas pelas análises de bancada (via úmida) e pelo uso do equipamento NIRS foram comparadas pelo teste T (dados pareados), ao nível de 5% de probabilidade, empregando-se o delineamento em blocos ao acaso. Houve diferença significativa nos nutrientes preditos pelo NIRS e analisados por via úmida (P≤0,05). O NIRS superestimou os teores de matéria seca e matéria mineral, mas subestimou os teores de FDN, FDA e PB. Esse resultado mostra que, quando do uso de modelos globais de calibração, esse equipamento não é capaz de predizer corretamente a composição químico-bromatológica de forrageiras de clima tropical.

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Temperature and soil moisture strongly affect the nutritional value and digestibility of forage plants through changes in leaf chemical composition or the proportion of leaf blade tissues. In this study, we aimed to evaluate leaf blade anatomical modifications of two tropical forage species, Stylosanthes capitata (C3) and Megathyrsus maximus (C4) under warmed conditions (+2 °C) at well-watered and rainfed conditions and investigate the interactions between leaf anatomical alterations, leaf chemical composition, and leaf digestibility. Experiments were conducted under field conditions using a Temperature-free air-controlled enhancement (T-FACE) system. We observed that plants under elevated temperature produced leaves with smaller stomata and thinner mesophyll tissue and reduced total leaf thickness, potentially impacting gas exchange. On the other hand, reduced soil moisture increased stomatal density and thickness of the adaxial epidermis. In both species, leaf fibrous fractions concentration increased under warmed and non-irrigated conditions, while crude protein concentration and digestibility decreased. However, leaf digestibility was associated with leaf chemical composition rather than the proportion of different leaf blade tissues. We concluded that although both species developed leaf anatomical modifications to acclimate under future warming conditions, leaf nutritional value and digestibility will be reduced, potentially impacting future livestock production and methane emissions by ruminants.

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Pastures based on perennial monocotyledonous plants are the principal source of nutrition for ruminant livestock in tropical and subtropical areas across the globe. The Urochloa genus comprises important species used in pastures, and these mainly include Urochloa brizantha, Urochloa decumbens, Urochloa humidicola, and Urochloa ruziziensis. Despite their economic relevance, there is an absence of genomic-level information for these species, and this lack is mainly due to genomic complexity, including polyploidy, high heterozygosity, and genomes with a high repeat content, which hinders advances in molecular approaches to genetic improvement. Next-generation sequencing techniques have enabled the recent release of reference genomes, genetic linkage maps, and transcriptome sequences, and this information helps improve our understanding of the genetic architecture and molecular mechanisms involved in relevant traits, such as the apomictic reproductive mode. However, more concerted research efforts are still needed to characterize germplasm resources and identify molecular markers and genes associated with target traits. In addition, the implementation of genomic selection and gene editing is needed to reduce the breeding time and expenditure. In this review, we highlight the importance and characteristics of the four main species of Urochloa used in pastures and discuss the current findings from genetic and genomic studies and research gaps that should be addressed in future research.

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ABSTRACT In order to estimate the base temperature (Bt) of growth through the appearance of leaves and calculate the phyllochron for kikuyu grass, three plots were established on three farms in the Provincia of Ubaté (Cundinamarca, Colombia) located at different altitudes (2560, 2640, 3143 m. a. s. l.). Measurements were made in four cycles in a period of eight months. The Bt was estimated by the least coefficient of variation method using a second order regression model and the model obtained was validated by the cross-validation method. The Bt values for the first, second, third and fourth leafwere 4.02, 3.68, 3.93, and 3.62 °C, respectively. For the appearance of the first leaf, the kikuyu required more thermal time (TT) (97.5 accumulated growing degree days (AGDD)) than for the second (74.2 AGDD), third (73.8 AGDD) and fourth leaf (76.0 AGDD) (p <0.05). There were no differences in TT among farms (p> 0.05). There was a tendency to a greater number of days required to reach each leaf stage in the farm located at higher altitude and with lower mean temperature. The validation showed an adequate adjustment (r2 = 0.94) and a substantial concordance (CCC = 0.97) between the observed values and the predicted values for the estimated TT with the Bt value obtained for each leaf stage. The results ofBt for kikuyu grass obtained, will allow to make more precise predictions about the phyllochron and generate growth models close to reality.

RESUMEN Con el fin de estimar la temperatura base (Tb) de crecimiento a través de la aparición de hojas y calcular el filocrono para el pasto kikuyo, se establecieron tres parcelas en tres fincas de la provincia de Ubaté (Cundinamarca, Colombia) ubicadas a diferente altitud (2560, 2640, 3143 m. s. n. m.). Se realizaron cuatro ciclos de mediciones en un tiempo total de ocho meses. La Tb se estimó por el método de mínimo coeficiente de variación utilizando un modelo de regresión de segundo grado y el modelo obtenido se validó por el método de validación cruzada. La Tb para la primera, segunda, tercera y cuarta hoja fue 4,02, 3,68, 3,93 y 3,62 °C, respectivamente. El kikuyo requirió mayor tiempo térmico (TT) para la aparición de la primera hoja (97,5 grados día de crecimiento acumulados (GDCA)) que para la segunda (74,2 GDCA), tercera (73,8 GDCA) y cuarta hoja (76,0 GDCA) (p<0,05). No hubo diferencias en el TT entre fincas (p > 0,05). Hubo tendencia a un mayor número de días calendario requeridos para alcanzar cada estado de hoja en la finca ubicada a mayor altitud y con menor temperatura media. La validación mostró un adecuado ajuste (r2 = 0,94) y una concordancia sustancial (CCC= 0,97) entre los valores observados y los valores predichos para el TT estimado con los valores de Tb obtenidos para cada estado de hoja. Los resultados de Tb de crecimiento del pasto kikuyo obtenidos permiten realizar predicciones más precisas sobre el filocrono y generar modelos de crecimiento cercanos a la realidad.

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This study evaluated the effect of energy supplementation with ground corn on the performance of crossbred dairy cows in BRS Kurumi elephant grass pastures managed under rotational stocking during the rainy season. Six Holstein × Gyr cows were used, with average milk production, body weight, body condition score, and days in milk of 18.0±2.89 kg day−1, 560±66 kg, 2.50±0.21, and 99±12, respectively. The experimental design used was the complete reversion (switchback), and the evaluations were conducted over three grazing cycles, with adaptation periods of 14 days and six days of sample collection. The concentrate supplement (ground corn) was supplied twice a day, at a rate of 2 kg cow−1 day−1 in the morning and 1 kg cow−1 day−1 in the afternoon (as-fed basis). A reduction of 23.4% was observed in the pasture dry matter (DM) intake (PDMI) in cows that received energy supplementation, which corresponds to 2.96 kg day-1 less of PDMI when compared to the group without supplementation. This corresponds to a substitution rate of 1.1 kg of pasture per kg of concentrate consumed (DM basis), which reduced the intake (kg cow−1 day−1) of neutral detergent fiber and crude protein by 18.9% and 13.9% in the cows that received ground corn. There were increases of 11.8%, 9.0%, and 10.1%, respectively, in the milk yield, the 3.5% fat-corrected milk yield, and the energy-corrected milk yield of the cows that received ground corn. The response to supplementation in kg of milk per kg of DM of concentrate consumed was 0.57, and the milk contents of fat, protein, and lactose did not differ between treatments. The energy supplementation with 3 kg cow−1 day−1 of ground corn resulted in an increase of 11.8% in the milk yield of Holstein × Gyr dairy cows grazed on BRS Kurumi elephant grass, as well as promoted increases in the daily protein, lactose, and total solids yields. In addition, the provision of 3 kg day−1 of ground corn for lactating cows grazing on BRS Kurumi increased the efficiency in the use of dietary nitrogen.(AU)

O objetivo do estudo foi avaliar o efeito da suplementação energética com milho moído sobre o desempenho de vacas em pastagem de capim-elefante BRS Kurumi manejado sob lotação rotacionada durante o período chuvoso. Foram utilizadas seis vacas Holandês x Gir, com produção média de leite, peso corporal, escore de condição corporal e dias em lactação de 18,0±2,89 kg dia−1, 560±66 kg, 2,50±0,21 e 99±12, respectivamente. O delineamento experimental utilizado foi o de reversão completa (switchback) e as avaliações foram realizadas durante três ciclos de pastejo, com períodos de adaptação de 14 dias, e seis dias de coletas de amostras. O suplemento concentrado (milho moído) foi fornecido duas vezes ao dia, à razão de 2 kg vaca−1 dia−1 pela manhã e 1 kg vaca−1 dia−1 à tarde (base da matéria natural). Observou-se redução de 23,4% no consumo de matéria seca (MS) de pasto nas vacas que receberam a suplementação energética, o que corresponde a menos 2,96 kg de MS de forragem dia-1, quando comparado ao grupo sem suplementação. Isto corresponde a uma taxa de substituição de 1,1 kg de pasto por kg de concentrado consumido (base da MS), o que reduziu o consumo (kg vaca−1 dia−1) de fibra em detergente neutro e proteína bruta em 18,9% e 13,9%, respectivamente, nas vacas que receberam milho moído. Foram observados aumentos de 11,8%, 9,0% e 10,1%, respectivamente, na produção de leite (PL), PL corrigida para 3,5% de gordura e PL corrigida para energia das vacas que receberam milho moído. A resposta à suplementação em kg de leite por kg de MS de concentrado consumido foi de 0,57, e os teores de gordura, proteína e lactose não diferiram entre tratamentos. A suplementação energética com 3 kg dia−1 de milho moído promoveu incremento de 11,8% na produção de leite de vacas Holandês x Gir em pastejo de capim-elefante BRS Kurumi, bem como aumento nas produções diárias de proteína, lactose e sólidos totais. Além disso, o fornecimento de 3 kg dia−1 de milho moído para vacas em lactação em pastejo de BRS Kurumi aumentou a eficiência na utilização do nitrogênio da dieta.(AU)

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To evaluate the influence of supplementation strategies on the average daily gain (ADG) of cattle in a rotational grazing system of Guinea grass (Panicum maximum cv. Coloniao) pastures during the rainy season. It was further evaluated the residual effects in the feedlot finishing phase. Seventy-five 8 month-old crossbred bull calves averaging 200.1 ± 2.5 kg of body weight (BW) were stratified and grouped in three into 25 blocks according to BW, and then randomly assigned to one of three supplementation treatments: control (no supplement), energy supplement [65 g crude protein (CP)/kg dry matter (DM)] or protein supplement (200 g CP/kg DM) both fed as 6 g/kg BW. All animals composed a single herd and were separated daily according to treatment groups for supplementation. After the grazing phase, all animals were moved to a feedlot and received the same diet up until slaughter. Guinea grass was subjected to rotational stocking with pre- and post-grazing heights of 76 and 43 cm, respectively. Energy and protein supplements provided similar ADG (P > 0.05; 0.94 kg/day) which was greater than for non-supplemented animals during the grazing phase (P ≤ 0.05; 0.74 kg/day), but there was no difference in the subsequent feedlot phase (P > 0.05; 1.45 kg/day). The protein supplement had no incremental effects over the energy supplement on ADG and carcass traits of growing crossbreed bulls during the grazing phase, indicating that either source could be used, and that an energy supplement would lead to more efficient nutrient use coming from the grass.(AU)

Objetivou-se avaliar estratégias de suplementação sobre o ganho médio diário (GMD) de bovinos pastejando capim-colonião (Panicum maximum) sob lotação rotacionada na estação chuvosa. Também foi avaliado o efeito residual na fase de terminação em confinamento. Setenta e cinco bezerros mestiços não-castrados de oito meses com peso corporal (PC) médio de 200,1 ± 2,5 kg foram estratificados e agrupados de acordo com PC, distribuídos em 25 blocos, e aleatorizados entre três tratamentos: controle (sem suplementação), suplemento energético [65 g de proteína bruta (PB)/kg matéria seca (MS)] ou suplemento proteico (200 g PB/kg MS) ambos fornecidos a 6 g/kg PC. Os animais compuseram um único rebanho sendo separados diariamente de acordo com o tratamento para suplementação. Após a fase de pastejo, esses foram movidos para um confinamento onde receberam a mesma dieta até o abate. O capim-colonião foi pastejado sob lotação rotacionada com altura média pré- e pós-pastejo de 76 e 43 cm, respectivamente. Os suplementos proporcionaram GMD semelhante (P > 0,05; 0,94 kg/dia) e superior ao dos animais não suplementados na fase de pastejo (P < 0,05; 0,74 kg/dia) e sem diferença na fase de confinamento (P > 0,05; 1,45 kg/dia). O suplemento proteico não proporciona ganho adicional sobre o energético, sem diferenças sobre o GMD e as características de carcaça de tourinhos mestiços durante a fase de pastejo, indicando que ambas as fontes de suplementação podem ser utilizadas baseado no custo e na disponibilidade, e indicando que o suplemento energético poderia proporcionar uso mais eficiente dos nutrientes contidos na forragem.(AU)

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The objective in this study was to evaluate the effects of supplementation levels on productive and nutritional performance and metabolic profile in suckling female calves under grazing. Forty female calves (averaging 3.5±0.06 months and 127.3±2.68 kg), and their respective dams were distributed in a completely randomized design with two treatments and twenty replicates. The treatments were 1) 4 g kg-1 body weight (BW) of supplement or 2) 6 g kg-1 BW of supplement. Forage and organic matter (OM) intake did not affect (P > 0.05) by levels of supplement, though crude protein and non-fibrous carbohydrates intake were greater (P < 0.05) by increasing supplementation level. There was no effect (P > 0.05) the supplementation levels on OM and CP digestibility. The metabolic profile of the animals was not affected (P > 0.05) by supplementation level. Average daily gain, longissimus dorsi area, fat thickness over rump of the animals did not affect (P > 0.05) by levels of supplement. However, there was trend of increasing (P=0.074) in fat thickness over loin by increase the supplementation level. Although the body growth of animals was similar (P > 0.05) between treatments, there was observed a trend of increase (P=0.064) in ratio BW:Height at the withers by increasing supplementation levels. In conclusion, increasing the supplementation level of 4 to 6 g kg-1 of BW, not improve the productive and nutritional performance and metabolic status in female calves under grazing on creep-feeding system.(AU)

O objetivo deste estudo foi avaliar os efeitos dos níveis de suplementação sobre o desempenho produtivo e nutricional e perfil metabólico de bezerras lactentes sob pastejo. Quarenta bezerras lactentes (média de 3,5 ± 0,06 meses e 127,3 ± 2,68 kg) e suas respectivas foram distribuídas em delineamento inteiramente casualizado com dois tratamentos e vinte repetições. Os tratamentos foram 1) 4 g kg-1 de peso corporal (PC) de suplemento ou 2) 6 g kg-1 PC de suplemento. O consumo de forragem e matéria orgânica (MO) não foi influenciado (P > 0,05) pelos níveis de suplemento, porém, o consumo de proteína bruta e carboidratos não-fibrosos foi aumentado (P < 0,05) pelo incremento do nível de suplemento. Não houve efeito (P > 0,05) dos níveis de suplementação sob a digestibilidade da MO e PB. O perfil metabólico dos animais não foi afetado pelo nível de suplementação (P > 0,05). O ganho médio diário, área de longissimus dorsi, espessura de gordura sobre a garupa dos animais não foram afetados (P > 0,05) pelos níveis de suplementação. No entanto, houve tendência de aumento (P=0,074) na espessura de gordura no lombo pelo aumento do nível de suplementação. Embora o crescimento corporal dos animais foi similar (P > 0,05) entre os tratamentos, observou-se uma tendência de aumento (P=0,064) na relação BW: Altura na cernelha, com o aumento do nível de suplementação, Concluindo, o aumento do nível de suplementação de 4 a 6 g kg-1 de PC, não melhora o desempenho produtivo e nutricional e estado metabólico em bezerras lactentes sob pastejo no sistema de creep feeding.(AU)

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The objective in this study was to evaluate the effects of supplementation levels on productive and nutritional performance and metabolic profile in suckling female calves under grazing. Forty female calves (averaging 3.5±0.06 months and 127.3±2.68 kg), and their respective dams were distributed in a completely randomized design with two treatments and twenty replicates. The treatments were 1) 4 g kg-1 body weight (BW) of supplement or 2) 6 g kg-1 BW of supplement. Forage and organic matter (OM) intake did not affect (P > 0.05) by levels of supplement, though crude protein and non-fibrous carbohydrates intake were greater (P 0.05) the supplementation levels on OM and CP digestibility. The metabolic profile of the animals was not affected (P > 0.05) by supplementation level. Average daily gain, longissimus dorsi area, fat thickness over rump of the animals did not affect (P > 0.05) by levels of supplement. However, there was trend of increasing (P=0.074) in fat thickness over loin by increase the supplementation level. Although the body growth of animals was similar (P > 0.05) between treatments, there was observed a trend of increase (P=0.064) in ratio BW:Height at the withers by increasing supplementation levels. In conclusion, increasing the supplementation level of 4 to 6 g kg-1 of BW, not improve the productive and nutritional performance and metabolic status in female calves under grazing on creep-feeding system.

O objetivo deste estudo foi avaliar os efeitos dos níveis de suplementação sobre o desempenho produtivo e nutricional e perfil metabólico de bezerras lactentes sob pastejo. Quarenta bezerras lactentes (média de 3,5 ± 0,06 meses e 127,3 ± 2,68 kg) e suas respectivas foram distribuídas em delineamento inteiramente casualizado com dois tratamentos e vinte repetições. Os tratamentos foram 1) 4 g kg-1 de peso corporal (PC) de suplemento ou 2) 6 g kg-1 PC de suplemento. O consumo de forragem e matéria orgânica (MO) não foi influenciado (P > 0,05) pelos níveis de suplemento, porém, o consumo de proteína bruta e carboidratos não-fibrosos foi aumentado (P 0,05) dos níveis de suplementação sob a digestibilidade da MO e PB. O perfil metabólico dos animais não foi afetado pelo nível de suplementação (P > 0,05). O ganho médio diário, área de longissimus dorsi, espessura de gordura sobre a garupa dos animais não foram afetados (P > 0,05) pelos níveis de suplementação. No entanto, houve tendência de aumento (P=0,074) na espessura de gordura no lombo pelo aumento do nível de suplementação. Embora o crescimento corporal dos animais foi similar (P > 0,05) entre os tratamentos, observou-se uma tendência de aumento (P=0,064) na relação BW: Altura na cernelha, com o aumento do nível de suplementação, Concluindo, o aumento do nível de suplementação de 4 a 6 g kg-1 de PC, não melhora o desempenho produtivo e nutricional e estado metabólico em bezerras lactentes sob pastejo no sistema de creep feeding.

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RESUMO

To evaluate the influence of supplementation strategies on the average daily gain (ADG) of cattle in a rotational grazing system of Guinea grass (Panicum maximum cv. Coloniao) pastures during the rainy season. It was further evaluated the residual effects in the feedlot finishing phase. Seventy-five 8 month-old crossbred bull calves averaging 200.1 ± 2.5 kg of body weight (BW) were stratified and grouped in three into 25 blocks according to BW, and then randomly assigned to one of three supplementation treatments: control (no supplement), energy supplement [65 g crude protein (CP)/kg dry matter (DM)] or protein supplement (200 g CP/kg DM) both fed as 6 g/kg BW. All animals composed a single herd and were separated daily according to treatment groups for supplementation. After the grazing phase, all animals were moved to a feedlot and received the same diet up until slaughter. Guinea grass was subjected to rotational stocking with pre- and post-grazing heights of 76 and 43 cm, respectively. Energy and protein supplements provided similar ADG (P > 0.05; 0.94 kg/day) which was greater than for non-supplemented animals during the grazing phase (P ≤ 0.05; 0.74 kg/day), but there was no difference in the subsequent feedlot phase (P > 0.05; 1.45 kg/day). The protein supplement had no incremental effects over the energy supplement on ADG and carcass traits of growing crossbreed bulls during the grazing phase, indicating that either source could be used, and that an energy supplement would lead to more efficient nutrient use coming from the grass.

Objetivou-se avaliar estratégias de suplementação sobre o ganho médio diário (GMD) de bovinos pastejando capim-colonião (Panicum maximum) sob lotação rotacionada na estação chuvosa. Também foi avaliado o efeito residual na fase de terminação em confinamento. Setenta e cinco bezerros mestiços não-castrados de oito meses com peso corporal (PC) médio de 200,1 ± 2,5 kg foram estratificados e agrupados de acordo com PC, distribuídos em 25 blocos, e aleatorizados entre três tratamentos: controle (sem suplementação), suplemento energético [65 g de proteína bruta (PB)/kg matéria seca (MS)] ou suplemento proteico (200 g PB/kg MS) ambos fornecidos a 6 g/kg PC. Os animais compuseram um único rebanho sendo separados diariamente de acordo com o tratamento para suplementação. Após a fase de pastejo, esses foram movidos para um confinamento onde receberam a mesma dieta até o abate. O capim-colonião foi pastejado sob lotação rotacionada com altura média pré- e pós-pastejo de 76 e 43 cm, respectivamente. Os suplementos proporcionaram GMD semelhante (P > 0,05; 0,94 kg/dia) e superior ao dos animais não suplementados na fase de pastejo (P 0,05; 1,45 kg/dia). O suplemento proteico não proporciona ganho adicional sobre o energético, sem diferenças sobre o GMD e as características de carcaça de tourinhos mestiços durante a fase de pastejo, indicando que ambas as fontes de suplementação podem ser utilizadas baseado no custo e na disponibilidade, e indicando que o suplemento energético poderia proporcionar uso mais eficiente dos nutrientes contidos na forragem.

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RESUMO

The aim of this study was to evaluate the effect of re-ensiling on the fermentability coefficient (FC), chemical composition and fermentation profile of untreated whole-crop sorghum silage after prolonged periods of environmental exposure. Treatments comprised eight times of exposure to the environment (0, 6, 12, 24, 48, 72, 96, and 120 h) and two procedures for conservation (conventional and re-ensiling) in a completely randomized design with two factors and three replicates. Experimental silos made of 12-L plastic buckets were used in trials. Silage nutritive value, fermentation profile, and dry mass (DM) losses were analyzed before and 90 days after re-ensiling. Regression analyses were performed, and ANOVA was used to compare means. The FC was higher than 45 even when the silage was exposed to air for 120 h before re-ensiling (59.2±2.54). Regression equations were fitted to the data with low accuracy (R2 < 0.47). Moreover, we observed that the main effect occurred between before and after re-ensiling, decreasing the contents of DM (42 to 37 %) and water-soluble carbohydrates (7.0 to 5.8 % DM), neutral detergent fiber (60.4 to 55.4 % DM), and acid detergent fiber (49.5 to 33.5 % DM), but increasing those of lactic acid (0.52 to 0.96 % DM) and ammoniacal nitrogen (1.58 to 2.51 % total N). The DM losses were linear with increasing times of air exposure; however, silage nutritive value and fermentation profile showed no disturbing changes for silage conservation and animal feeding.

O objetivo com este estudo foi avaliar o efeito da reensilagem sobre o coeficiente de fermentação (CF), bromatologia e fermentação de silagens de plantas inteiras de sorgo após períodos de exposição ao ambiente. Os tratamentos corresponderam a oito tempos de exposição ao ambiente (0, 6, 12, 24, 48, 72, 96 e 120 h) e dois procedimentos de conservação (silagem convencional e reensilada), no delineamento inteiramente casualizado com dois fatores e três repetições, utilizando-se silos experimentais de 12 L. Os silos foram abertos 90 dias após o fechamento e analisados o valor nutritivo, o perfil fermentativo e as perdas de massa seca (MS). Foram ajustadas regressões e a ANOVA foi utilizada para comparação entre médias. O CF foi maior do que 45, mesmo quando a silagem foi exposta ao ambiente por 120 h antes a reensilagem (59,2±2,54). As equações de regressão foram ajustadas com baixa precisão (R2 < 0,47) e foi observado que o principal efeito ocorreu entre os procedimento de conservação e não para os tempos de exposição, reduzindo o conteúdo de MS (42 para 37 %) e carboidratos solúveis em água (7,0 para 5,8 % da MS), fibras em detergente neutro (60,4 para 55,4 % da MS) e ácido (49,5 para 33,5 % da MS), e aumentando os teores de AL (0,52 para 0,96 % da MS) e N-NH3 (1,58 para 2,51 % do N total). As perdas de MS foram lineares com o tempo de exposição ao ambiente, no entanto, não foram observadas mudanças impactantes no valor nutritivo das silagens com o processo de reensilagem.

Assuntos

RESUMO

The aim of this study was to evaluate the effect of re-ensiling on the fermentability coefficient (FC), chemical composition and fermentation profile of untreated whole-crop sorghum silage after prolonged periods of environmental exposure. Treatments comprised eight times of exposure to the environment (0, 6, 12, 24, 48, 72, 96, and 120 h) and two procedures for conservation (conventional and re-ensiling) in a completely randomized design with two factors and three replicates. Experimental silos made of 12-L plastic buckets were used in trials. Silage nutritive value, fermentation profile, and dry mass (DM) losses were analyzed before and 90 days after re-ensiling. Regression analyses were performed, and ANOVA was used to compare means. The FC was higher than 45 even when the silage was exposed to air for 120 h before re-ensiling (59.2±2.54). Regression equations were fitted to the data with low accuracy (R2 < 0.47). Moreover, we observed that the main effect occurred between before and after re-ensiling, decreasing the contents of DM (42 to 37 %) and water-soluble carbohydrates (7.0 to 5.8 % DM), neutral detergent fiber (60.4 to 55.4 % DM), and acid detergent fiber (49.5 to 33.5 % DM), but increasing those of lactic acid (0.52 to 0.96 % DM) and ammoniacal nitrogen (1.58 to 2.51 % total N). The DM losses were linear with increasing times of air exposure; however, silage nutritive value and fermentation profile showed no disturbing changes for silage conservation and animal feeding.(AU)

O objetivo com este estudo foi avaliar o efeito da reensilagem sobre o coeficiente de fermentação (CF), bromatologia e fermentação de silagens de plantas inteiras de sorgo após períodos de exposição ao ambiente. Os tratamentos corresponderam a oito tempos de exposição ao ambiente (0, 6, 12, 24, 48, 72, 96 e 120 h) e dois procedimentos de conservação (silagem convencional e reensilada), no delineamento inteiramente casualizado com dois fatores e três repetições, utilizando-se silos experimentais de 12 L. Os silos foram abertos 90 dias após o fechamento e analisados o valor nutritivo, o perfil fermentativo e as perdas de massa seca (MS). Foram ajustadas regressões e a ANOVA foi utilizada para comparação entre médias. O CF foi maior do que 45, mesmo quando a silagem foi exposta ao ambiente por 120 h antes a reensilagem (59,2±2,54). As equações de regressão foram ajustadas com baixa precisão (R2 < 0,47) e foi observado que o principal efeito ocorreu entre os procedimento de conservação e não para os tempos de exposição, reduzindo o conteúdo de MS (42 para 37 %) e carboidratos solúveis em água (7,0 para 5,8 % da MS), fibras em detergente neutro (60,4 para 55,4 % da MS) e ácido (49,5 para 33,5 % da MS), e aumentando os teores de AL (0,52 para 0,96 % da MS) e N-NH3 (1,58 para 2,51 % do N total). As perdas de MS foram lineares com o tempo de exposição ao ambiente, no entanto, não foram observadas mudanças impactantes no valor nutritivo das silagens com o processo de reensilagem.(AU)

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RESUMO

Fungal endophytes of Brachiaria, a nonhost of Sclerotinia sclerotiorum, may harbor species with antagonistic effects against this plant pathogen. The objective of this work was to investigate the diversity of endophytic fungi associated with different Brachiaria species and hybrids and evaluate their potential to inhibit the plant pathogen S. sclerotiorum. Stem samples from 39 Brachiaria spp. plants were collected in pasture fields and experimental areas of three states of Brazil resulting in 74 endophytes isolated. Twenty-eight species were identified by sequences of the Internal Transcribed Spacer (ITS) and 18S rDNA regions. Paraconiothyrium sp. was the most abundant endophyte, accounting for 24 % (14 isolates) of total, and it was isolated from B. ruziziensis, B. decumbens, B. humidicola, and B. brizantha. Phoma sorghina was the second most abundant taxon, followed by Sarocladium strictum, and Plenodomus sp. In vitro analyses showed that Paraconiothyrium sp., Sarocladium kiliense, Acremonium curvulum, Setophoma terrestris, Dissoconium sp., and Cladosporium flabelliforme exhibited antagonistic activity against S. sclerotiorum, with percentages of growth inhibition ranging from 25 to 60 (p < 0.05). Paraconiothyrium sp. BBXE1 (60 %), BBPB4.1 (60 %), BCMT4.1 (54 %), and S. kiliense (54 %) showed the highest values of Antagonism Percentages (AP). Therefore, fungi with inhibitory activity against S. sclerotiorum such as Paraconiothyrium sp. are naturally endophytic in Brachiaria grasses.(AU)

Assuntos

RESUMO

Fungal endophytes of Brachiaria, a nonhost of Sclerotinia sclerotiorum, may harbor species with antagonistic effects against this plant pathogen. The objective of this work was to investigate the diversity of endophytic fungi associated with different Brachiaria species and hybrids and evaluate their potential to inhibit the plant pathogen S. sclerotiorum. Stem samples from 39 Brachiaria spp. plants were collected in pasture fields and experimental areas of three states of Brazil resulting in 74 endophytes isolated. Twenty-eight species were identified by sequences of the Internal Transcribed Spacer (ITS) and 18S rDNA regions. Paraconiothyrium sp. was the most abundant endophyte, accounting for 24 % (14 isolates) of total, and it was isolated from B. ruziziensis, B. decumbens, B. humidicola, and B. brizantha. Phoma sorghina was the second most abundant taxon, followed by Sarocladium strictum, and Plenodomus sp. In vitro analyses showed that Paraconiothyrium sp., Sarocladium kiliense, Acremonium curvulum, Setophoma terrestris, Dissoconium sp., and Cladosporium flabelliforme exhibited antagonistic activity against S. sclerotiorum, with percentages of growth inhibition ranging from 25 to 60 (p < 0.05). Paraconiothyrium sp. BBXE1 (60 %), BBPB4.1 (60 %), BCMT4.1 (54 %), and S. kiliense (54 %) showed the highest values of Antagonism Percentages (AP). Therefore, fungi with inhibitory activity against S. sclerotiorum such as Paraconiothyrium sp. are naturally endophytic in Brachiaria grasses.

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RESUMO

The opening and closing of stomata are controlled by the integration of environmental and endogenous signals. Here, we show the effects of combining elevated atmospheric carbon dioxide concentration (eCO 2; 600 µmol mol-1) and warming (+2°C) on stomatal properties and their consequence to plant function in a Stylosanthes capitata Vogel (C3) tropical pasture. The eCO 2 treatment alone reduced stomatal density, stomatal index, and stomatal conductance (gs ), resulting in reduced transpiration, increased leaf temperature, and leading to maintenance of soil moisture during the growing season. Increased CO2 concentration inside leaves stimulated photosynthesis, starch content levels, water use efficiency, and PSII photochemistry. Under warming, plants developed leaves with smaller stomata on both leaf surfaces; however, we did not see effects of warming on stomatal conductance, transpiration, or leaf water status. Warming alone enhanced PSII photochemistry and photosynthesis, and likely starch exports from chloroplasts. Under the combination of warming and eCO 2, leaf temperature was higher than that of leaves from the warming or eCO 2 treatments. Thus, warming counterbalanced the effects of CO2 on transpiration and soil water content but not on stomatal functioning, which was independent of temperature treatment. Under warming, and in combination with eCO 2, leaves also produced more carotenoids and a more efficient heat and fluorescence dissipation. Our combined results suggest that control on stomatal opening under eCO 2 was not changed by a warmer environment; however, their combination significantly improved whole-plant functioning.

RESUMO

This study was designed to evaluate the effects of different supplementation plans on the nutrient intake, apparent total-tract digestibility, grazing behavior, growth performance, and carcass characteristics of beef cattle under grazing conditions from ages 4 to 18 months old. The beef calves grazed Brachiaria decumbens in four seasons: rainy-dry transition, dry, dry-rainy transition, and rainy. Forty-four animals (11 per treatment) were randomly assigned to one of four nutritional plans of supplementation defined by the quantity of supplement offered: control, low, medium, and high supplementation. There was no difference (P > 0.10) in dry matter intake (DMI). However, animals receiving medium and high supplementation had decreased (P < 0.10) forage dry matter intake (FDMI) compared with those under non- and low supplementation. The DMI and FDMI were lower (P < 0.10) in the dry season. During the rainy season, the grazing time decreased (P < 0.10) for animals receiving supplementation compared with non-supplemented ones, but was similar between medium and high supplementation. The performance and carcass characteristics were greater (P < 0.10) for high and medium supplementation compared with low and control. In conclusion, increasing the supplementation plans for beef cattle in tropical pastures increases the nutrient intake but decreases FDMI. The performance also increases with the supplementation plan; however, the growth rate is affected by the grazing season.(AU)

Este estudo foi conduzido para avaliar os efeitos de diferentes planos de suplementação sobre o consumo de nutrientes, digestibilidade aparente total, comportamento ingestivo, crescimento e características de carcaça de bovinos de corte de 4 a 18 meses de idade, em pastejo. Os animais foram mantidos em pastagem com Brachiaria decumbens em quatro períodos: transição águas-seca, seca, transição seca-águas e águas. Quarenta e quatro animais (11 por tratamento) foram distribuídos aleatoriamente em um dos quatro planos nutricionais de suplementação definidos pela quantidade de suplemento ofertada: controle (somente mistura mineral), baixo, médio e alto plano de suplementação. Não houve diferença (P > 0,10) no consumo de matéria seca (CMS). No entanto, os animais que receberam médio e alto plano de suplementação reduziram (P < 0,10) o consumo de matéria seca de forragem (CMSF) comparado ao controle e baixo plano. O CMS e CMSF foram menores (P < 0,10) durante o período seco. Durante a estação chuvosa, o tempo de pastejo reduziu (P < 0,10) para os animais que receberam suplementação em comparação ao controle, mas foi semelhante entre os planos médio e alto. O desempenho e as características de carcaça foram maiores (P < 0,10) para o alto e médio plano comparados ao baixo e controle. Conclui-se que o aumento nas suplementações para bovinos de corte em pastagens tropicais aumenta a ingestão de nutrientes, mas diminui o consumo de forragem. O desempenho também aumenta com o plano de suplementação; no entanto, a taxa de crescimento é afetada pela época do ano.(AU)

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